Biofilm Formation on Implants and Prosthetic Dental Materials

2016 ◽  
pp. 991-1027
Author(s):  
Lia Rimondini ◽  
Andrea Cochis ◽  
Elena Varoni ◽  
Barbara Azzimonti ◽  
Antonio Carrassi
Keyword(s):  
Biofilm Formation ◽  
Dental Materials

scholarly journals Predictability of Microbial Adhesion to Dental Materials by Roughness Parameters

Coatings ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 456 ◽  
Author(s):  
Andrea Schubert ◽  
Torsten Wassmann ◽  
Mareike Holtappels ◽  
Oliver Kurbad ◽  
Sebastian Krohn ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Titanium Alloy ◽  
Biofilm Formation ◽  
Dental Materials ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Mean Deviation ◽  
Roughness Parameters ◽  
Microbial Adhesion

Microbial adhesion to intraoral biomaterials is associated with surface roughness. For the prevention of oral pathologies, smooth surfaces with little biofilm formation are required. Ideally, appropriate roughness parameters make microbial adhesion predictable. Although a multitude of parameters are available, surface roughness is commonly described by the arithmetical mean roughness value (Ra). The present study investigates whether Ra is the most appropriate roughness parameter in terms of prediction for microbial adhesion to dental biomaterials. After four surface roughness modifications using standardized polishing protocols, zirconia, polymethylmethacrylate, polyetheretherketone, and titanium alloy specimens were characterized by Ra as well as 17 other parameters using confocal microscopy. Specimens of the tested materials were colonized by C. albicans or S. sanguinis for 2 h; the adhesion was measured via luminescence assays and correlated with the roughness parameters. The adhesion of C. albicans showed a tendency to increase with increasing the surface roughness—the adhesion of S. sanguinis showed no such tendency. Although Sa, that is, the arithmetical mean deviation of surface roughness, and Rdc, that is, the profile section height between two material ratios, showed higher correlations with the microbial adhesion than Ra, these differences were not significant. Within the limitations of this in-vitro study, we conclude that Ra is a sufficient roughness parameter in terms of prediction for initial microbial adhesion to dental biomaterials with polished surfaces.

scholarly journals Biofilm formation on dental materials

2018 ◽  
Vol 34 (77) ◽  
pp. 1821-1831
Author(s):  
Jana Stanković-Pešić ◽  
Milena Kostić ◽  
Marko Igić ◽  
Vukica Đorđević
Keyword(s):  
Biofilm Formation ◽  
Dental Materials

Biofilm Formation on Implants and Prosthetic Dental Materials

2015 ◽  
pp. 1-37 ◽  
Author(s):  
Lia Rimondini ◽  
Andrea Cochis ◽  
Elena Varoni ◽  
Barbara Azzimonti ◽  
Antonio Carrassi
Keyword(s):  
Biofilm Formation ◽  
Dental Materials

Evaluation of an Adhesive Containing Calcium Salt of Acidic Monomers on Inhibition of Biofilm Formation of Bacteria Related to Root Caries

2020 ◽  
Vol 853 ◽  
pp. 41-45
Author(s):  
Sroisiri Thaweboon ◽  
Takashi Saito ◽  
Keiji Nagano ◽  
Boonyanit Thaweboon
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Calcium Salt ◽  
Dental Materials ◽  
The Elderly ◽  
Cell Counting ◽  
Tooth Surface ◽  
Bottom Surface ◽  
Root Caries ◽  
Flat Bottom

Root caries is a critical dental problem for the elderly people due to increased tooth retention and exposed root surfaces in aged society. The presence of a cariogenic biofilm and fermentable carbohydrates is the main etiologic factors. Streptococcus mutans, lactobacilli and Actinomyces spp. have been demonstrated to be associated with root caries. Currently, dental materials with multifunctional features have been combined into clinical adhesives. Calcium salt of acidic monomer (calcium salt of 4-methacryloxyethyl trimellitic acid or CMET) has been demonstrated to promote a significant remineralization and an adhesive containing CMET has been developed. However, there is no data regarding its antimicrobial potential. This study aimed to evaluate the property of an adhesive containing CMET and 10-methacryloyloxydecyl dihydrogen calcium phosphate (MDCP) on the biofilm formation of bacteria related to root caries. The adhesive was applied onto the flat-bottom surface of 96-well plate and LED light-cured. Then it was coated with sterile saliva at 37 °C for 60 min. The bacterial suspensions of 107 colony forming unit (CFU)/mL (Streptococcus mutans ATCC 25715, Lactobacillus casei ATCC 334 and Actinomyvces viscosus ATCC 19246) were added and incubated at 37°C in 5% CO2 atmosphere for 24 h to allow the biofilm formation. The amount of vital biofilm was determined by WST-8 Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA). All tests were carried out in triplicate and repeated three times. Student’s t-test was employed for the statistical analysis. The significant suppressive effects were clearly noticed on S. mutans compared with a control. The percentage of biofilm reduction was nearly 65%. A 2% biofilm reduction was found on L. casei and A. viscosus, though no statistical significant decreases of biofilm were observed compared with a control. In conclusion, an adhesive containing calcium salt of acidic monomers could significantly inhibit biofilm formation of S. mutans. Using this adhesive can be beneficial for the prevention of root caries on the tooth surface.

scholarly journals Inhibitory effect of a gel paste containing surface pre-reacted glass-ionomer (S-PRG) filler on the cariogenicity of Streptococcus mutans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryota Nomura ◽  
Takahiro Kitamura ◽  
Saaya Matayoshi ◽  
Jumpei Ohata ◽  
Yuto Suehiro ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Bacterial Growth ◽  
Biofilm Formation ◽  
Dental Materials ◽  
Self Care ◽  
Dependent Manner ◽  
Glass Ionomer ◽  
Inhibitory Effects ◽  
Concentration Dependent Manner

AbstractSurface pre-reacted glass-ionomer (S-PRG) filler is a bioactive functional glass that releases six different ions. Although several dental materials containing S-PRG filler have been developed, few self-care products containing S-PRG filler have been reported. We investigated the inhibitory effects of PRG gel paste containing S-PRG filler on Streptococcus mutans, a major pathogen of dental caries. PRG gel paste inhibited bacterial growth of S. mutans in a concentration-dependent manner, and all S. mutans were killed in the presence of ≥ 1% PRG gel paste. Additionally, it was difficult for S. mutans to synthesize insoluble glucan from sucrose in the presence of 0.1% PRG gel paste. A biofilm formation model was prepared in which slices of bovine enamel were infected with S. mutans after treatment with or without PRG gel paste. Biofilm formation was inhibited significantly more on the enamel treated with PRG gel paste than on enamel without PRG gel paste (P < 0.001). The inhibitory effects on bacterial growth and biofilm formation were more prominent with PRG gel paste than with S-PRG-free gel paste, suggesting that PRG gel paste may be effective as a self-care product to prevent dental caries induced by S. mutans.

scholarly journals Silver Nanoparticles in Dental Biomaterials

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Juliana Mattos Corrêa ◽  
Matsuyoshi Mori ◽  
Heloísa Lajas Sanches ◽  
Adriana Dibo da Cruz ◽  
Edgard Poiate ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Biofilm Formation ◽  
Dental Materials ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Filler Level ◽  
Over Time

Silver has been used in medicine for centuries because of its antimicrobial properties. More recently, silver nanoparticles have been synthesized and incorporated into several biomaterials, since their small size provides great antimicrobial effect, at low filler level. Hence, these nanoparticles have been applied in dentistry, in order to prevent or reduce biofilm formation over dental materials surfaces. This review aims to discuss the current progress in this field, highlighting aspects regarding silver nanoparticles incorporation, such as antimicrobial potential, mechanical properties, cytotoxicity, and long-term effectiveness. We also emphasize the need for more studies to determine the optimal concentration of silver nanoparticle and its release over time.

scholarly journals Antimicrobial Poly (methyl methacrylate) with Silver Nanoparticles for Dentistry: A Systematic Review

2020 ◽  
Vol 10 (11) ◽  
pp. 4007 ◽  
Author(s):  
Flores-Arriaga Juan Carlos ◽  
García-Contreras Rene ◽  
Villanueva-Sánchez Germán ◽  
Acosta-Torres Laura Susana
Keyword(s):  
Silver Nanoparticles ◽  
Methyl Methacrylate ◽  
Biofilm Formation ◽  
Dental Materials ◽  
Etiologic Factor ◽  
Poly Methyl Methacrylate ◽  
Oral Infections ◽  
Dental Prostheses ◽  
Antibacterial And Antifungal ◽  
Dental Applications

Poly(methyl methacrylate) (PMMA) is a widely used polymer for dental applications, and it is mainly used in the fabrication of dental prostheses. In an increasing number of these applications, the risk of suffering bacterial or fungal infection is higher than 60% among oral-prosthesis users. Some authors have reported the failure of other implants in the human body due to biofilm formation on the surface (mainly for total hip implants). In the dental field, the formation of bacterial and fungal biofilms on prosthesis’s surface is the etiologic factor for stomatitis, mainly caused by Candida albicans and bacteria such as Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa and Enterococcus faecalis, as well as many others. The antibacterial and antifungal properties of silver nanoparticles (AgNPs) have been widely reported, and their use in dental materials can prevent oral infections, such as candidiasis and stomatitis, and promote better oral health in dental-prosthesis users. They can even be used in other biomedical applications that require controlling biofilm formation on surfaces. In this review, the reported studies that use composites of PMMA and AgNPs (PMMA-AgNPs) for dental applications are listed and checked, with the aim of gaining a wider perspective of the use and application of this approach in the dental field.

scholarly journals Bio-Interactive Zwitterionic Dental Biomaterials for Improving Biofilm Resistance: Characteristics and Applications

2020 ◽  
Vol 21 (23) ◽  
pp. 9087
Author(s):  
Utkarsh Mangal ◽  
Jae-Sung Kwon ◽  
Sung-Hwan Choi
Keyword(s):  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Dental Materials ◽  
Causative Factor ◽  
Oral Biofilm ◽  
Zwitterionic Polymers ◽  
Oral Biofilms ◽  
Protein Adhesion ◽  
Microbial Environment ◽  
Significant Attention

Biofilms are formed on surfaces inside the oral cavity covered by the acquired pellicle and develop into a complex, dynamic, microbial environment. Oral biofilm is a causative factor of dental and periodontal diseases. Accordingly, novel materials that can resist biofilm formation have attracted significant attention. Zwitterionic polymers (ZPs) have unique features that resist protein adhesion and prevent biofilm formation while maintaining biocompatibility. Recent literature has reflected a rapid increase in the application of ZPs as coatings and additives with promising outcomes. In this review, we briefly introduce ZPs and their mechanism of antifouling action, properties of human oral biofilms, and present trends in anti-biofouling, zwitterionic, dental materials. Furthermore, we highlight the existing challenges in the standardization of biofilm research and the future of antifouling, zwitterated, dental materials.

scholarly journals Comparison of Fungal Biofilm Formation on Three Contemporary Denture Base Materials

2015 ◽  
Vol 4 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Russell Wicks ◽  
Jegdish Babu ◽  
Franklin Garcia-Godoy ◽  
Vinay Jain
Keyword(s):  
Biofilm Formation ◽  
Dental Materials ◽  
Dry Weight ◽  
Xtt Assay ◽  
Denture Base ◽  
Viability Assay ◽  
Base Materials ◽  
Tissue Responses ◽  
Weight Analysis ◽  
Relative Potential

ABSTRACT Statement of problem: Modern polyamide ‘flexible’ denture base materials have increased in popularity for use in removable partial dentures in the last several years. The introduction of these newer products warrants investigation of their relative potential to develop fungal biofilms. Purpose The purpose of this study was to investigate the potential of three denture base materials to support fungal biofilm formation. Materials and methods Specimens of two ‘flexible’ nylon type materials and one traditional heat processed, methyl methacrylate resin material were studied (both polished and unpolished surfaces). The specimens were coated with saliva and evaluated for fungal (Candida albicans) biofilm formation. The fungal biofilm mass formed on denture substrates were evaluated by dry weight analysis and by determining the number of viable fungal cells in the biofilm by MTT viability assay. Alteration in fungal metabolic function following the treatment of the biofilm C. albicans with nystatin and fluconazole was determined by XTT assay. Results In general, the unpolished surfaces of the denture disks favored the fungal biofilm, the most being on polyamide specimen, Valplast. Significantly, less biofilm was formed on Duraflex and Lucitone surfaces. Biofim on C. albicans was also found to be resistant to antifungal agents. As compared to freshly incubated (grown) planktonic cells, biofilm fungal cells required significantly higher concentrations of nystatin and fluconazole in order to obtain 50% reduction in metabolic activity. Conclusion This study demonstrated the differences in denture materials to support fungal biofilm formation, and also difference between polished and unpolished denture material surfaces. The results demonstrated that one of the polyamide materials (duraflex) had lesser potential to biofilm formation than the others. Clinical significance Unfavorable tissue responses can ensue from the presence of fungal biofilms on dental prosthetics. Resistance to biofilm formation is a factor for dental materials in their selection and usage. This study helps to quantify, evaluate and compare biofilm formation on polished and unpolished surfaces of three commonly used denture base materials. The results of this study helped to identify materials, which may, therefore, be better indicated in clinical applications. Evaluations for the newer denture base materials, specific to these testing methods, appear to be novel in the scientific literature. How to cite this article Jain V, Babu J, Ahuja S, Wicks R, Garcia-Godoy F. Comparison of Fungal Biofilm Formation on Three Contemporary Denture Base Materials. Int J Experiment Dent Sci 2015;4(2):104-108.

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