Uptake of 32P from labelled organic matter, by mycorrhizal and non-mycorrhizal subterranean clover (Trifolium subterraneum L.)

1995 ◽  
Vol 172 (2) ◽  
pp. 221-227 ◽  
Author(s):  
Erik J Joner ◽  
Iver Jakobsen

2011 ◽  
Vol 62 (12) ◽  
pp. 1078 ◽  
Author(s):  
X. Li ◽  
R. L. Ison ◽  
R. C. Kellaway ◽  
C. Stimson ◽  
G. Annison ◽  
...  

A range of annual legume genotypes comprising one line of Trifolium subterraneum, four lines of T. michelianum, 11 of T. resupinatum var. resupinatum, and one line of T. resupinatum var. majus were grown in glasshouses under temperature regimes of 10−15°C and 16−21°C. Dry matter (DM) weights of stem, leaf, and flower tissues were measured when plants had six nodes, at first flower appearance, and at senescence. All samples were scanned by near-infrared reflectance spectroscopy (NIRS). One-third of the samples, covering the range of spectral characteristics, were analysed for in vitro digestible organic matter (DOMD), organic matter, crude protein (CP), neutral detergent fibre (NDF), lignin, cellulose, and the hemicellulosic polysaccharide monomers arabinose, xylose, mannose, galactose, and rhamnose. These data were used to develop calibration equations from which the composition of the remaining samples was predicted by NIRS. The higher temperature resulted in plants reaching respective phenological stages earlier, but did not affect either DM yields of total plant, stem, leaf, and petiole tissues or the proportions of each fraction. In vitro DOMD and arabinose and galactose levels decreased, while lignin, cellulose, NDF, xylose, mannose, and rhamnose levels increased with advancing maturity. In vitro DOMD was positively associated with contents of CP, arabinose, galactose, and the arabinose/xylose ratio and was negatively associated with contents of lignin, cellulose, NDF, xylose, mannose, and rhamnose. Lignin contents were highly correlated with levels of both xylose and mannose. Stems were more digestible than leaves in subterranean clover and T. resupinatum var. majus. The study also demonstrated that NIRS can be used routinely as a quick, inexpensive, and reliable laboratory technique to predict feed components of annual Trifolium legumes.



1986 ◽  
Vol 26 (6) ◽  
pp. 665 ◽  
Author(s):  
PER Ridley ◽  
HL Davies ◽  
IN Southey

Medium Peppin Merino wethers were fed, ad libitum, subterranean clover (cv. Woogenellup), rose clover (cv. Kondinin) and soft brome grass cut in September (spring), November (late spring) and January-February (summer). The nitrogen contents of the subterranean clover ranged from 1.89 to 3.13%, of the rose clover from 1.62 to 2.84% and of the soft brome from 0.42 to 2.86%. Mean daily organic matter intakes (kg) in spring, late spring and summer were respectively: for subterranean clover 0.78, 1.09 and 0.42; for rose clover 0.60, 0.87 and 0.62; for soft brome grass 0.87, 0.81 and 0.20. Organic matter digestibilities in spring, late spring and summer were, respectively: for subterranean clover 0.82 ,0.69 and 0.61; for rose lover 0.80, 0.62 and 0.54; for soft brome 0.83, 0.72 and 0.58. There were highly significant differences ( P < 0.001) in nitrogen balance between seasons. The estimated digestible energy intakes (MJ/day) in spring, late spring and summer were, respectively: for subterranean clover 12.7, 14.5 and 4.9; for rose clover, 9.2, 9.9 and 6.9; for soft brome, 13.6, 10.1 and 2.1. Within seasons there were significant (P < 0.05) differences in nitrogen balance between species. There were significant differences between species in mean retention time in the alimentary tract in late spring (P<0.001) and summer (P<0.001) but not in spring. There was a highly significant negative relationship between acid-detergent fibre content and both organic matter and fibre digestibilities. The superior digestible energy intakes of the 2 clovers explain at least some of the superior performance of sheep grazing clover compared with those grazing grass pastures.



1962 ◽  
Vol 59 (3) ◽  
pp. 349-353 ◽  
Author(s):  
G. R. Pearce ◽  
J. E. Vercoe ◽  
D. E. Tribe

1. Recordings of faecal organic matter (O.M.), faecal N% (O.M. basis) and live weight, were made over 52 consecutive weekly periods on ten Corriedale wethers grazing a pasture consisting mainly of Wimmera rye-grass (Lolium rigidum Gaud.) with some subterranean clover (Trifolium subterraneum) in a Mediterranean-type environment. The amount of available O.M. and its N% were also recorded.2. By applying regressions of (i) the ratio of O.M. feed intake to O.M. faecal output on faecal N% and (ii) the N% in the O.M. intake on faecal N%, to the above observations it was possible to estimate the following quantities: O.M. and digestible organic matter (D.O.M.) intake, N and digestible N intake and O.M. digestibility percentage.3. The patterns of O.M. and D.O.M. intake, and N and digestible N intake are described. Similar patterns exist for each of these quantities. Two maxima, a large one in the spring and a smaller one in the autumn, and two minima, one in the summer and one in the winter, are exhibited. The values for these various maxima and minima are presented in the text.4. Attention is drawn to variations from the general pattern for these quantities and factors which could influence these patterns are mentioned.



1991 ◽  
Vol 31 (6) ◽  
pp. 785 ◽  
Author(s):  
AR Leys ◽  
B Plater ◽  
WJ Lill

The responses of vulpia [Vulpia bromoides (L.) S. F. Gray and V. myuros (L.) C. C. Gmelin] and subterranean clover (Trifolium subterraneum L.) to rate and time of application of simazine were compared on a red loam soil containing 1.8% organic matter and on sands containing 0.4 and 0.8% organic matter. In 1988 and 1989, applications of 0.50, 0.75 and 1.00 kg a.i./ha of simazine in late May-early June resulted in 96.4, 99.8 and 99.6% control of vulpia, respectively. Later applications in July-August, or earlier applications in April, were less effective. There were no differences in the levels of control obtained on the 2 soils, nor were there any differences in the susceptibility of V. bromoides or V. myuros to simazine. On the red loam soil simazine did not reduce subterranean clover plant density or dry matter production; however, severe reductions of both were recorded on the sandy soils. Reasons for the different responses, along with the potential for the use of simazine to control vulpia in subterranean clover pastures, are discussed in detail.



1957 ◽  
Vol 8 (2) ◽  
pp. 179 ◽  
Author(s):  
CH Williams ◽  
CM Donald

A further investigation has been made on soils examined by Donald and Williams (1955) in a survey of the influence of superphosphate and subterranean clover (Trifolium subterraneum L.) on podzolic soils formed on granodiorite in the Crookwell district of New South Wales. The soil organic matter was found to have approximately constant proportions of carbon, nitrogen, sulphur, and phosphorus in the ratio 155 : 10 : 1.4 : 0.68, and it is suggested that the rate of build-up of soil organic matter under the clover pastures may have been limited by the amounts of sulphur supplied in the superphosphate top-dressings. The soil organic matter was found to have a cation-exchange capacity a t pH 7.0 of about 220 m-equiv./100 g, and increases in soil organic matter have led to substantial increases in the cation-exchange capacity of the soil. About 75 per cent. of the total cation-exchange capacity of the unimproved soils was due to organic matter while, in the most improved soils, this figure approached 90 per cent. The increases in cation-exchange capacity included increases in exchangeable hydrogen and were accompanied by decreases in soil pH. There were increases equivalent to 6.5 lb of exchangeable potassium, 25.5 Ib of exchangeable calcium, and 5.2 lb of exchangeable magnesium per acre in the surface 4 in. of soil for each hundredweight of superphosphate applied per acre. Increases in soil organic matter also resulted in increases in the field capacity which may reflect improvements in soil structure. Results indicate that heavier rates of superphosphate application would increase the rate of fertility build-up.



1962 ◽  
Vol 59 (3) ◽  
pp. 343-348 ◽  
Author(s):  
J. E. Vercoe ◽  
G. R. Pearce ◽  
D. E. Tribe

1. Regression equations for the ratio of organic matter (O.M.) feed intake to O.M. faecal output on faecal N%, and the N% in the O.M. intake on faecal N% were established over a 52-week period by feeding caged Corriedale wethers on pasture cut from a Wimmera rye-grass (Lolium rigidum Gaud.), subterranean clover (Trifolium subterraneum) sward in a Mediterranean-type environment.2. The regression equations and their application to grazing sheep together with the relation between O.M. intake and faecal N, are discussed.



2000 ◽  
Vol 51 (3) ◽  
pp. 377 ◽  
Author(s):  
G. M. Lodge

Seedlings of 3 perennial grasses, Danthonia linkii Kunthcv. Bunderra, D. richardsonii Cashmore cv. Taranna(wallaby grasses), and Phalaris aquatica L. cv. Sirosa,were each grown in replacement series mixtures with seedlings ofTrifolium repens L. (white clover),Trifolium subterraneum L. var. brachycalycinum (Katzn.et Morley) Zorahy & Heller cv. Clare (subterraneanclover), and Lolium rigidum L. (annual ryegrass). Plantswere sown 5 cm apart in boxes (45 by 29 by 20 cm) at a density of 307plants/m2. Maximum likelihood estimates were usedto derive parameters of a non-linear competition model using the dry matterweights of perennial grasses and competitors at 3 harvests, approximately 168,216, and 271 days after sowing. Intra-plant competition was examined inmonocultures of each species, grown at plant spacings of 2, 5, and 8 cm apartwith plants harvested at the above times.Competition occurred in all perennial grass–competitor mixtures, exceptin those of each perennial grass with white clover and thephalaris–subterranean clover mixture (Harvest 1) and those withD. richardsonii and phalaris grown with white clover(Harvest 2). For D. richardsonii (Harvests 1 and 2) andD. linkii (Harvest 1 only) grown with white clover andthe phalaris–subterranean clover (Harvest 1), the two species in themixture were not competing. In the phalaris–white clover mixture, eachspecies was equally competitive (Harvests 1 and 2). These differences incompetition and aggressiveness reflected differences in individual plantweights in monocultures where there was an effect (P < 0.05) of species ondry matter weight per box, but no significant effect of plant spacing.These data indicated that for successful establishment,D. richardsonii and D. linkiishould not be sown in swards with either subterranean clover or white clover,or where populations of annual ryegrass seedlings are likely to be high.Phalaris was more compatible with both white clover and subterranean clover,but aggressively competed with by annual ryegrass.



2004 ◽  
Vol 94 (5) ◽  
pp. 433-439 ◽  
Author(s):  
S.N. Johnson ◽  
P.J. Gregory ◽  
P.J. Murray ◽  
X Zhang ◽  
I.M. Young

AbstractThis study investigated the ability of neonatal larvae of the root-feeding weevil, Sitona lepidus Gyllenhal, to locate white clover Trifolium repens L. (Fabaceae) roots growing in soil and to distinguish them from the roots of other species of clover and a co-occurring grass species. Choice experiments used a combination of invasive techniques and the novel technique of high resolution X-ray microtomography to non-invasively track larval movement in the soil towards plant roots. Burrowing distances towards roots of different plant species were also examined. Newly hatched S. lepidus recognized T. repens roots and moved preferentially towards them when given a choice of roots of subterranean clover, Trifolium subterraneum L. (Fabaceae), strawberry clover Trifolium fragiferum L. (Fabaceae), or perennial ryegrass Lolium perenneL. (Poaceae). Larvae recognized T. repens roots, whether released in groups of five or singly, when released 25 mm (meso-scale recognition) or 60 mm (macro-scale recognition) away from plant roots. There was no statistically significant difference in movement rates of larvae.



1956 ◽  
Vol 7 (2) ◽  
pp. 98 ◽  
Author(s):  
JN Black

Changes in the pre-emergence distribution of dry matter in subterranean clover (Trifolium subterraneum L.) variety Bacchus Marsh were followed at 21°C, using three sizes of seed and three depths of sowing, ½, 1¼, and 2 in. Decreasing seed size and increasing depth of sowing both reduce the weight of the cotyledons a t emergence. Seed of the three sizes were sown a t three depths in pot culture a t staggered intervals so that emergence was simultaneous. Dry weight in the early vegetative stage was proportional to seed size, and total leaf area and leaf numbers showed similar trends. Plants of each seed size grew at the same relative rate. No effect of depth of sowing could be detected, and this was shown to be due to the cotyledon area a t emergence being constant for any given seed size, regardless of varying depth of sowing and hence of cotyledon weight. It was concluded that seed size in a plant having epigeal germination and without endosperm is of importance: firstly, in limiting the maximum hypocotyl elongation and hence depth of sowing, and secondly, in determining cotyledon area. Cotyledon area in turn influences seedling growth, which is not affected by cotyledon weight. Once emergence has taken place, cotyledonary reserves are of no further significance in the growth of the plants.



Author(s):  
H. Neave ◽  
C.H. Hewlett

The information contained in this paper is principally first-hand knowledge extending over a period of six years gained from personal experience from our own "Oakleigh" farm.



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