High-speed electro-fusion and electro-transfection of plant protoplasts by a continuous flow electro-manipulator

1988 ◽  
Vol 7 (3) ◽  
pp. 153-157 ◽  
Author(s):  
T. Hibi ◽  
H. Kano ◽  
M. Sugiura ◽  
T. Kazami ◽  
S. Kimura
Keyword(s):  
Continuous Flow ◽  
High Speed ◽  
Plant Protoplasts

Chemical and Clinical Evaluation of the Continuous-flow Analyzer "SMAC"

1974 ◽  
Vol 20 (8) ◽  
pp. 1062-1070 ◽  
Author(s):  
Morton K Schwartz ◽  
Victor G Bethune ◽  
Martin Fleisher ◽  
Gina Pennacchia ◽  
Celia J Menendez-Botet ◽  
...  
Keyword(s):  
Continuous Flow ◽  
High Speed ◽  
Total Bilirubin ◽  
Carbon Dioxide Content ◽  
Protein Matrix ◽  
Sodium Potassium ◽  
Calcium Phosphorus ◽  
Matrix Reference Material ◽  
Computer Controlled ◽  
Speed Computer

Abstract "SMAC" (Sequential Multiple Analyzer plus Computer) is a high-speed computer-controlled multitest analyzer. A 20-channel prototype SMAC (glucose, urea nitrogen, creatinine, carbon dioxide content, total bilirubin, calcium, phosphorus, cholesterol, iron, uric acid, chloride, sodium, potassium, total protein, albumin, creatine kinase, alkaline phosphatase, lactate dehydrogenase, and aspartate and alanine aminotransferases) has been evaluated for: (a) method precision during within-day runs and on a day-to-day basis over a period of time; (b) method linearity over a range established on a chemical basis and related to clinical requirements, with use of both aqueous standards and protein matrix reference material; and (c) correlation of SMAC values with those obtained by the methods routinely in use in our department.

Development and Evaluation of a High-Speed Continuous-Flow Analyzer

1972 ◽  
Vol 18 (9) ◽  
pp. 1013-1018
Author(s):  
M A Evenson ◽  
M A Olson
Keyword(s):  
Continuous Flow ◽  
High Speed ◽  
High Performance ◽  
Operating Characteristics ◽  
Raw Data ◽  
Automatic Computer ◽  
Carry Over ◽  
First Time

Abstract A high-speed, high-performance, continuous-flow analyzer is described that operates at two to three times the usual analysis rate without necessitating corrections of the raw data and with no decrease in accuracy or precision. At faster speeds (180-300 samples/h) inductive sample interaction (%Ii), opposite in direction to carry-over, is for the first time quantitatively measured. A correction equation for %Ii was developed, and when it is applied to raw data, the accuracy of the results are significantly improved. Operating characteristics of the high-speed analyzer are described and the desirability of automatic computer corrections is discussed for the high-speed system.

Design and Performance of a Miniaturized High-Speed Continuous-Flow Analyzer

1974 ◽  
Vol 20 (4) ◽  
pp. 424-427 ◽  
Author(s):  
William E Neeley ◽  
Stephen C Wardlaw ◽  
Helen C Sing
Keyword(s):  
Sample Size ◽  
Continuous Flow ◽  
High Speed ◽  
Clinical Laboratory ◽  
Small Animal ◽  
Sample Volume ◽  
Small Sample ◽  
Reagent Consumption ◽  
And Performance ◽  
Made In

Abstract Design features and performance of a miniaturized high-speed continuous-flow analyzer are described. Special emphasis is made in the design towards a system that is free from the operational and mechanical complexities found in most of today’s advanced systems. Depending on the particular analyses, sample size varies from 3 to 25 µl and reagent consumption is less than 180 µl per sample. Analyses are performed under steady-state conditions at sampling rates of 150 samples per hour with a 2:1 or 3:1 sample-to-wash ratio. The marked reduction in sample size makes the system ideal for microanalyses, especially in the pediatric clinical laboratory, in small animal research, and in any other cases where small sample volume is especially important.

Improved method for measurement of inorganic phosphate in serum with a centrifugal analyzer.

1976 ◽  
Vol 22 (2) ◽  
pp. 257-260 ◽  
Author(s):  
P W Wentz ◽  
J Savory ◽  
R E Cross
Keyword(s):  
Plasma Cell ◽  
Data Reduction ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
High Speed ◽  
Improved Method ◽  
Plasma Cell Dyscrasias

Abstract A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric and data-reduction capabilities of the centrifugal analyzer. Hemolysis, icterus, and moderate lipemia cause no interference. Grossly lipemic sera and sera from patients with plasma cell dyscrasias can be analyzed by incorporating appropriate blanking and dilution techniques. The method exhibits excellent sensitivity and precision and results correlate well with those from a continuous-flow procedure.

P1838Thrombocytopenia under ECMO and Shear-induced shedding of platelet receptor Glycoprotein-(GP)Ialpha

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
F Vincent ◽  
A Rauch ◽  
M Desvages ◽  
H Spillemaeker ◽  
N Rousse ◽  
...  
Keyword(s):  
Platelet Count ◽  
Continuous Flow ◽  
High Speed ◽  
Time Dependent ◽  
Loop Model ◽  
High Shear ◽  
Significant Time ◽  
Platelet Receptor ◽  
Flow Device

Abstract Background Several mechanisms are suspected to thrombocytopenia under Extracorporeal Membrane Oxygenation (ECMO) such as platelet-consumption or sepsis. Shedding of glycoprotein-(GP)Ibα is a recently identified mechanism of platelet clearance. ECMO generates high shear stress forces that could impact GPIbα-shedding. We hypothesized that ECMO continuous-flow devices could directly induce thrombocytopenia through shear-induced GPIbα-shedding. Aims Determine if ECMO induce GpIb-shedding in vitro and in vivo and determinates the kinetic evolution of platelet-count and GpIb-shedding after patient's implantation. Methods Platelet GPIbα-shedding was first investigated in vitro using a high-shear pump loop model. Plasma with normal platelet count (plasma-NPC) was obtained by dilution of platelet-rich plasma obtained from healthy donors in fresh-frozen-plasma. Samples were collected before and after (5, 30, 60 and 180 min) perfusion at 37°C of plasma-NPC at intermediate and high speed (2.6 and 3.6 L min–1 respectively, n=4 each). Platelet count and GPIbα-shedding were next investigated in 20 ECMO patients before/after implantation (WITECMO trial) and in 20 healthy volunteers. The geometric mean-fluorescence-intensity (gMFI) of platelet GPIbα (PE-staining) and GPIX (FITC-staining) was measured with a Navios flow cytometer (Beckman Coulter, Miami, FL). Results are expressed as GPIbα/GPIX gMFI-ratio. Results A significant time-dependent loss of GPIbα/GPIX gMFI-ratio was already apparent after 30 min in vitro and was significantly more pronounced at high-speed compared to intermediate-speed (pANOVA<0.001 and p<0.01 at 180 min respectively). GPIbα/GPIX gMFI-ratio was significantly increased in ECMO patients compared to healthy subjects 1- and 24-hour after implantation (p<0.001). A significantly lower platelet count was observed 1 hour after ECMO implantation (−23% vs baseline, p<0.01) with a further significant decrease at 24-hours (−53% vs baseline, p<0.0001). Figure 1. A. Significant time-dependent loss of platelet GPIbα/GPIX gMFI-ratio (pANOVA <0.001) assessed by flow-cytometry after 30 min of perfusion at 3.6 L/min with a high-shear continuous-flow device in vitro. B. Representative experiment showing the apparition of a platelet sub-population with loss of GPIbα expression after 30 min of perfusion at 3.6 L/minwith a high-shear continuous-flow device in vitro.

Continuous-flow analysis for glucose, triglycerides, and ATP with immobilized enzymes in tubular form.

1977 ◽  
Vol 23 (9) ◽  
pp. 1556-1562 ◽  
Author(s):  
L P León ◽  
M Sansur ◽  
L R Snyder ◽  
C Horvath
Keyword(s):  
Continuous Flow ◽  
High Speed ◽  
Colorimetric Assay ◽  
Flow Analysis ◽  
Immobilized Enzymes ◽  
Free Enzyme ◽  
Assay Sensitivity ◽  
Biochemical Analyzer ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Continuous Use

Abstract Small-bore ("Autozyme") tubes with immobilized enzymes at the inner wall have been developed and studied for application in the Technicon "SMAC" high-speed continuous-flow biochemical analyzer. Tubes coated with glucose oxidase (D-glucose:oxygen oxidoreductase, EC 1.1.3.4) have been prepared for the assay of glucose, with colorimetric assay of the hydrogen peroxide produced; tubes coated with glycerol kinase (ATP:glycerol phosphotransferase, EC 2.7.1.30) for the enzymatic assay of triglycerides; tubes coated with hexokinase (ATP:D-hexose-6-phosphotransferase, EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NAD+ oxidoreductase EC 1.1.1.49) for the measurement of ATP, an intermediate product in assays for creatine kinase. With use of 10-15 cm lengths of Autozyme tube and SMAC hydraulics (150 samples per hour), assay sensitivity and carryover were similar to values for the corresponding free-enzyme methods. These immobilized enzymes were sufficiently stable for one to eight weeks of continuous use before replacemnt. We conclude that suitable bound-enzyme tubes can replace either single or multiple free-enzyme reagents in many continuous-flow assays at high sampling rates.

scholarly journals The so-called thermoid effect and the question of superheating of a plantinum-silver resistance used in continuous-flow calorimetry

1912 ◽  
Vol 86 (587) ◽  
pp. 330-332 ◽  
Keyword(s):  
Specific Heat ◽  
Continuous Flow ◽  
High Speed ◽  
Electric Heating ◽  
Flow Calorimeter ◽  
Heating Current ◽  
Liquid Moving ◽  
Absolute Measurements ◽  
Good Agreement ◽  
Made In

In a recent paper by Glazebrook, Bousfield, and Smith some doubt has been east on the accuracy of my absolute measurements of the specific heat of water by the continuous-flow calorimeter. It is stated that an error of as much as 6 parts in 10,000 might have occurred in the superheating of the oil-stirred platinurn-silver resistances from which the values of the electric heating current were obtained. On the other band, it is also stated that this error might have been as small as 2.5 parts in 10,000. It was with some surprise that I read this statement, inasmuch as the authors could not have been aware of the rapidity of oil circulation which I used. It has been shown by Osborne Reynolds and others that the heat loss from a surface immersed in a liquid moving in turbulent motion is directly proportional to the velocity of flow. The degree of superheating of a wire immersed in oil will depend then directly on the rate of circulation, bully realising this fact, the resistances which I used were designed by Prof. Callendar and myself to be immersed in oil which could be circulated with great rapidity, These resistances are described in our papers, where the illustration shows approximately to scale the relative sizes of the stirrer and resistances. The paddle was rotated at a high speed by a powerful water motor, and the oil was thrown down with such force that a considerable depression was made in the surface. The oil, thrown sideways, passed up around the bars wires, which were wound loosely on the mica frames. I do not know by what standard Glazebrook, Bousfield, and Smith decide what is "normal stirring" or "very vigorous stirring," but it was evident to me at ones that I must have bad much more rapid circulation than anything used by these authors. In testing the accuracy of my experiments special attention was taken of possible superheating, and tests of stirring were made at the time. The good agreement of the various determinations of the specific heat, made with such different values for the heating current and flow of water, make evident that no large superheating error could have existed without being detected.

scholarly journals Simulation Research of Hydraulic Stepper Drive Technology Based on High Speed On/Off Valves and Miniature Plunger Cylinders

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 438
Author(s):  
Hanyu Qiu ◽  
Qi Su
Keyword(s):  
Mathematical Model ◽  
Continuous Flow ◽  
High Speed ◽  
Single Step ◽  
Performance Parameters ◽  
State Control ◽  
Excellent Performance ◽  
Simulation Research ◽  
Step Step ◽  
Flow Medium

The technology for stepper drive that can achieve accurate motion in the hydraulic field has always been urgently needed in the industry. This paper proposes a hydraulic stepper drive based on five high speed on/off valves and two miniature plunger cylinders. The stepper drive discretizes the continuous flow medium into fixed small steps through the miniature plunger cylinder and realizes the state control of the drive through the logic action of the high speed on/off valve. This paper improves the current stepper drive and establishes a mathematical model to analyze the error of the drive and calculate the position of the actuator. In addition, through simulation research, the performance parameters such as the single-step step characteristic and pressure characteristic of the stepper drive are studied. The results show that, compared with the technology of current stepper drive, this stepper drive can effectively remove the “post step” phenomenon, greatly improve the stepper accuracy of the stepper drive, and have a more excellent performance.

Interference by tubing material in continuous-flow systems.

1978 ◽  
Vol 24 (12) ◽  
pp. 2170-2171 ◽  
Author(s):  
E B de Jong ◽  
H M Goldschmidt ◽  
J A Loog ◽  
C C van Alphen
Keyword(s):  
Stainless Steel ◽  
Continuous Flow ◽  
High Speed ◽  
Anomalous Behavior ◽  
Flow Systems ◽  
Tubing Material

Abstract An anomalous behavior in the measurement of calcium with the smac multi-channel high-speed analyzer was eliminated by taking the diluted serum directly from the dilution loop of the instrument before it enters the stainless-steel riser.

Development of a High-Pressure, High-Temperature, Optically Accessible Continuous-Flow Vessel for Fuel-Injection Experiments

2014 ◽  
Vol 136 (9) ◽  
Author(s):  
Kemar C. James ◽  
Jin Wang ◽  
Michael C. Maynard ◽  
Zackery B. Morris ◽  
Brian T. Fisher
Keyword(s):  
High Pressure ◽  
Continuous Flow ◽  
High Speed ◽  
Fuel Injection ◽  
Light Emitting Diode ◽  
Cone Angle ◽  
High Speed Camera ◽  
Light Emitting ◽  
High Pressure High Temperature ◽  
Spray Visualization

A vessel has been designed for nonreacting fuel-injection experiments with continuous flow of sweep gas at pressures up to 1380 kPa and temperatures up to 200 °C. Four orthogonal windows provide optical access for high-speed spray-visualization using a fast-pulsed light emitting diode (LED) and a high-speed camera. Initial experiments have been conducted to determine spray characteristics of n-heptane. At room conditions, liquid length and cone angle were 170 mm and 14.5 deg, respectively. With air flow in the chamber at 690 kPa and 100 °C, liquid length was considerably shorter at 92 mm and cone angle was wider at 16.5 deg.

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