The specificity of the lymphocyte transformation test in a patient with hypersensitivity reactions to pyrazolone compounds. A 10-week follow-up study before and after rechallenge

1988 ◽  
Vol 35 (1) ◽  
pp. 39-45 ◽  
Author(s):  
N. W. Brattig ◽  
G. -J. Diao ◽  
P. A. Berg
Keyword(s):  
Lymphocyte Transformation ◽  
Lymphocyte Transformation Test ◽  
Hypersensitivity Reactions ◽  
Follow Up Study ◽  
Before And After

scholarly journals TO010A NEW IMMUN-TOXICOLOGICAL TEST TO DETECT POLYSULFONE HYPERSENSITIVITY IN HEMODIALYSIS PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Joachim Beige ◽  
Ralph Wendt ◽  
Despina Rüssmann ◽  
Karl-Peter Ringel
Keyword(s):  
False Negative ◽  
False Negative Rate ◽  
Lymphocyte Transformation ◽  
Clinical Problem ◽  
Lymphocyte Transformation Test ◽  
Laboratory Research ◽  
Humoral Immune ◽  
Before And After ◽  
Positive Results

Abstract Background and Aims Incompatibility of dialysis procedure due to hypersensitivity against dialyzer material which currently is mainly based on polysulfone and derivatives can not be assessed by routine laboratory tests. Although the frequency of such symptoms is suspected to be low (below 2%) such resembles an important clinical problem because dialysis procedures are frequently accompanied by symptoms of non-tolerability with reasons not being entirely clear while circulatory reasons are suspected to play a major role. Method To enlighten the role of polysulfone hypersensitivity, we adapted known standardized material immune-toxicological tests (lymphocyte transformation test, basophil degranulation test) to the specific conditions of dialysis and polysulfone material sensitivity. We developed a method of polysulfone micronisation and measured humoral immune response of isolated patient´s lymphocytes when incubated with polysulfone dispersion. Results 39 samples from 103 patients with suspected polysulfone hypersensitivity showed positive results for type 1 (n=19), type 4 (n=18) or both type (n=2) reactions. There were no significant differences in the level of stimulation measured for DI, SI and lymphogenesis before and after dialysis (average delta -0.4; -0.28; - 1.74, p = 0.71; 0.34; 0.37) and with different dialyzer materials (Tab. 1). Patients with pos. type 4 results (LTT and lymphogenesis) showed highly correlated results in either LTT or lymphogenesis test (Fig. 1, R=0.87, p<0.0001). 8 out of 8 samples from patients with repeated test on different PS showed positive results on either PS. One patient tested positive on PS showed no hypersensitivity with another non-PS (PMMA) material. Conclusion This is the first methodological report showing plausible in-vitro results of patients samples concerning polysulfone intolerance. On the first superficial view, a “false-negative” rate of 60% looks rather disappointing, because all samples derived from patients with suspicion of PS hypersensitivity. However, due to the clinical variability of intolerance symptoms and the high prevalence of any problems after HD initiation, mainly of circulatory origin after initiating extracorporeal circuit, this rate may obviously express the true frequency of isolated PS material hypersensitivity in suspected patients. Alternative pathophysiological pathways of material sensitivity like complement activation, remain to be elucidated and incorporated into a comprehensive future testing panel. Further clinical and laboratory research is needed to define true polysulfone hypersensitivity and to enlighten the field of hypothetic subclinical material incompatibility in patients with impaired dialysis tolerability.

scholarly journals Impact of COVID-19 on child malnutrition, obesity in women and household food insecurity in underserved urban settlements in Sri Lanka: a prospective follow up study

2021 ◽  
pp. 1-24
Author(s):  
Renuka Jayatissa ◽  
Himali Herath ◽  
Amila Gayan Perera ◽  
Thulasika Thejani Dayaratne ◽  
Nawmali Dhanuska De Alwis ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Food Insecurity ◽  
Child Malnutrition ◽  
Nutrition Status ◽  
Household Food Insecurity ◽  
Follow Up Study ◽  
Household Food ◽  
Before And After ◽  
Urban Settlements

Abstract Objectives: To determine changes and factors associated with child malnutrition, obesity in women and household food insecurity before and after the first wave of COVID-19 pandemic. Design: A prospective follow up study. Setting: In 2019, the baseline Urban Health and Nutrition Study (UHNS-2019) was conducted in 603 households, which were selected randomly from 30 clusters to represent underserved urban settlements in Colombo. In the present study, 35% of households from the UHNS-2019 cohort were randomly selected for repeat interviews, one year after the baseline study and 6 months after COVID-19 pandemic in Sri Lanka. Height/length and weight of children and women were re-measured, household food insecurity was reassessed, and associated factors were gathered through interviewer administered questionnaires. Differences in measurements at baseline and follow-up studies were compared. Participants: A total of 207 households, comprising 127 women and 109 children were included. Results: The current prevalence of children with wasting and overweight was higher in the follow-up study than at baseline UHNS-2019 (18.3%vs13.7%;p=0.26 and 8.3%vs3.7%;p=0.12 respectively). There was a decrease in prevalence of child stunting (14.7%vs11.9%;p=0.37). A change was not observed in overall obesity in women, which was around 30.7%. Repeated lockdown was associated with a significant reduction in food security from 57% in UHNS-2019 to 30% in the current study (p<0.001). Conclusions: There was an increase in wasting and overweight among children while women had a persistent high prevalence of obesity. This population needs suitable interventions to improve nutrition status of children and women to minimise susceptibility to COVID-19.

scholarly journals Whether Keratectasia Area Shown in Corneal Topography Is Appropriate for Evaluating the Effect of Corneal Cross-Linking for Keratoconus: A 12-Month Follow-Up Study

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Jia Wang ◽  
Zhiwei Li ◽  
Huankai Zhang ◽  
Ning Gao ◽  
Guoying Mu
Keyword(s):  
Endothelial Cell ◽  
Linear Regression ◽  
Early Stage ◽  
Morphological Changes ◽  
Corneal Thickness ◽  
Corneal Topography ◽  
Cross Linking ◽  
Follow Up Study ◽  
Before And After

Purpose. To analyze the keratectasia area (KEA) shown in corneal topography before and after corneal cross-linking (CXL) in patients with progressive keratoconus (KC) and figure out whether KEA is appropriate for evaluating the effect of CXL. Methods. A retrospective analysis was conducted in 34 eyes from 24 progressive KC patients who have underwent CXL from 2015 to 2017. Area with K-value more than 47D shown in the corneal topography was marked and identified as KEA. Keratometry (K1, K2, and Kmax), KEA, thinnest corneal thickness (TCT), and endothelial cell density (ECD) were evaluated preoperatively or at months 3, 6, and 12 postoperatively. The changes of KEA before and after operation were evaluated. The relation of KEA and other parameters, including Kmax and TCT, was analyzed. Results. Linear regression model revealed the KEA, Kmax, K1, and K2 decreased after CXL in model y = 0.9622 -0.02408 x (P<0.05), y = 0.9982 -0.003469 x(P<0.05), y = 0.9977 + -0.001347 x(P<0.05), y = 0.9992 + -0.001779 x(P<0.05) (y represents KEA, Kmax, K1, or K2; x represents time (month)). The KEA is significantly decreased in early stage (before month 3) (P<0.05); however, the Kmax, K1, and K2 have no significant decrease in early stage (P= 0.09, 0.19, 0.32). Conclusions. The KEA is more sensitive than K-value in describing the morphological changes of cornea after CXL, especially in early stage after treatment.

scholarly journals EFFECTS OF PSYCHOTROPICS ON NEUTROPHIL-LYMPHOCYTE RATIO IN PSYCHIATRIC PATIENTS

2019 ◽  
pp. 210-213
Author(s):  
RAJESH VENKATARAMAN ◽  
MUHAMMED RASHID ◽  
AKHILA G ◽  
SHIJO DANIEL ◽  
AMALATHOMAS
Keyword(s):  
Bipolar Disorder ◽  
Complete Blood Count ◽  
Drug Effect ◽  
Psychiatric Patients ◽  
Newly Diagnosed ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Follow Up Study ◽  
Before And After

Objective: The objective of this study was to investigate whether psychotropic has any effect on neutrophils and lymphocyte ratio (NLR) in psychiatric patients. Methods: In this hospital-based prospective quasi-randomized follow-up study, we analyzed every 10 patients with newly diagnosed with depression, schizophrenia, and bipolar disorder for complete blood count before and after treatment with the selected psychotropic. Results: There was a significant increase in NLR of patients with depression after treatment with psychotropic (p=0.001), also in schizophrenia, there was a slight increase in NLR of patients after treatment with psychotropic (p=0.005), but in bipolar disorder, there was a significant decrease in NLR of patients after treatment with psychotropic (p=0.002). Conclusion: The present study confirmed that psychotropics have a statistically significant effect on the increase in NLR in patients with depression, a slight increase in patients with schizophrenia, and a decrease in patients with bipolar disorder. Further studies are needed for a better understanding of the mechanism of psychotropic drug effect on NLR.

Depression and monocyte dysfunction: a follow-up study

1994 ◽  
Vol 9 (6) ◽  
pp. 293-298 ◽  
Author(s):  
S Cervera-Enguix ◽  
A Rodriguez-Rosado
Keyword(s):  
Immune Response ◽  
Depressive Symptoms ◽  
Phagocytic Activity ◽  
Longitudinal Design ◽  
Opioid Activity ◽  
Follow Up Study ◽  
Hla Dr ◽  
Before And After ◽  
Vimentin Filaments

SummarySeveral studies have reported immune cellular and humoral dysfunction during depression. We specifically focused on the study of the monocyte as it has a key role in the activation of the immune response. To examine the association between severity of depressive symptoms and values of monocyte parameters (HLA-DR, CD35, phagocytic activity and vimentin filaments), we used a longitudinal design and assessed monocyte markers at intake and at follow-up 12 weeks after discharge from the hospital in 49 depressed patients. Seventy percent of patients showed pretreatment a marked monocyte dysfunction (82.5% had at least one parameter altered). After treatment, alterations in immunological variables were significantly associated (P < 0.05) with depression scores higher than 15. The findings indicate that the monocyte dysfunction is temporally associated with the state of depression. Before and after treatment the immunoreactive vimentin filaments significantly increased (P < 0.01) after incubation of monocytes with naloxone, suggesting that an increased opioid activity might account for the monocyte dysfunction.

Protein and amino acid status before and after bariatric surgery: A 12-month follow-up study

2013 ◽  
Vol 9 (6) ◽  
pp. 1008-1012 ◽  
Author(s):  
Carolina Ferreira Nicoletti ◽  
Marcia Varella Morandi Junqueira-Franco ◽  
Jose Ernesto dos Santos ◽  
Julio Sergio Marchini ◽  
Wilson Salgado Junior ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Amino Acid ◽  
Follow Up Study ◽  
Before And After ◽  
Acid Status

scholarly journals Improving the Recording of Diagnoses in Primary Care with Team Incentives: A Controlled Longitudinal Follow-Up Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Tuomo Lehtovuori ◽  
Timo Kauppila ◽  
Jouko Kallio ◽  
Anna M. Heikkinen ◽  
Marko Raina ◽  
...  
Keyword(s):  
Primary Care ◽  
Interrupted Time Series ◽  
Interrupted Time Series Analysis ◽  
Follow Up Study ◽  
Primary Care Teams ◽  
Before And After ◽  
Quasi Experimental ◽  
Clinically Significant ◽  
Made In

Introduction. We studied whether primary care teams respond to financial group bonuses by improving the recording of diagnoses, whether this intervention leads to diagnoses reflecting the anticipated distribution of diseases, and how the recording of a significant chronic disease, diabetes, alters after the application of these bonuses. Methods. We performed an observational register-based retrospective quasi-experimental follow-up study with before-and-after setting and two control groups in primary healthcare of a Finnish town. We studied the rate of recorded diagnoses in visits to general practitioners with interrupted time series analysis. The distribution of these diagnoses was also recorded. Results. After group bonuses, the rate of recording diagnoses increased by 17.9% (95% CI: 13.6–22.3) but not in either of the controls (−2.0 to −0.3%). The increase in the rate of recorded diagnoses in the care teams varied between 14.9% (4.7–25.2) and 33.7% (26.6–41.3). The distribution of recorded diagnoses resembled the respective distribution of diagnoses in the former studies of diagnoses made in primary care. The rate of recorded diagnoses of diabetes did not increase just after the intervention. Conclusions. In primary care, the completeness of diagnosis recording can be, to varying degrees, influenced by group bonuses without guarantee that recording of clinically significant chronic diseases is improved.

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