Changes in the protein content in hypothalamic neurons of neonatally castrated sexually mature male rats

1978 ◽  
Vol 86 (5) ◽  
pp. 1503-1506
Author(s):  
N. A. Borisova ◽  
K. Chandraseckhar
Keyword(s):  
Protein Content ◽  
Mature Male ◽  
Male Rats ◽  
Hypothalamic Neurons ◽  
Sexually Mature

Sperm protamine levels as indicators of fertilising potential in sexually mature male rats

Andrologia ◽  
2008 ◽  
Vol 40 (1) ◽  
pp. 29-37 ◽  
Author(s):  
M. Aleem ◽  
V. Padwal ◽  
J. Choudhari ◽  
N. Balasinor ◽  
M. K. Gill-Sharma
Keyword(s):  
Mature Male ◽  
Male Rats ◽  
Sexually Mature

scholarly journals Morphofunctional State of the Mature Rats Pituitary Gland under Readaptation Conditions to the Long Influence of Heavy Metal Salts Complex and Correction with L-Tocopherol

2021 ◽  
Vol 6 (6) ◽  
pp. 245-252
Author(s):  
N. B. Hryntsova ◽  
◽  
A. M. Romanyuk ◽  
O. O. Tymakova ◽  
D. R. Khrin
Keyword(s):  
Heavy Metal ◽  
Pituitary Gland ◽  
Mature Male ◽  
Metal Salts ◽  
Male Rats ◽  
Structural Components ◽  
Adaptive Processes ◽  
Heavy Metal Salts ◽  
Sexually Mature ◽  
Experimental Group

The purpose of the study was to study the morphofunctional rearrangements of the structural components of the adenohypophysis of sexually mature male rats under the condition of a 30-day period of readaptation to the long-term influence of salts of heavy metals (zinc, copper, iron, manganese, lead and chromium) and taking a corrector drug. Materials and methods. The experiment was carried out on 24 white sexually mature male rats, which were divided into 1 control and 1 experimental group. The experimental group consisted of rats that consumed ordinary drinking water for 30 days after 90 days of receiving water saturated with a combination of heavy metal salts: zinc (ZnSO4 7H2O) – 5 mg / l, copper (CuSO4 5H2O) mg / l, iron (FeSO4) – 10 mg / l, manganese (MnSO4 5H2O) – 0.1 mg / l, lead (Pb (NO3) 2) – 0.1 mg / l and chromium (K2Cr2O7) – 0.1 mg / l and L-tocopherol corrector. Results and discussion. With a 30-day period of readaptation to the action of a complex of salts of heavy metals, combined with the intake of a corrector drug L-tocopherol, morphological rearrangements of a nonspecific polymorphic nature developed in the structural components of the pituitary gland: compensatory hypertrophy and increased vascularization of the pituitary gland were observed, the state of rheological properties of the blood improved, the number of chromophiles increased and the number of chromophobes decreased, the processes of cyst formation disappeared, the stress from the adaptive processes in the glandulocytes of the adenohypophysis decreased, namely their secretory activity, the expression of Hsp90α receptors in the cytoplasm of adenocytes significantly increased. Negative rearrangements include an increased value of the fibrous component of the connective tissue of the intertrabecular spaces, increased collagenization of the wall of veins and capillaries, preservation of stromal edema, plethora of hemocapillaries, the presence of a small number of adenocytes with signs of vacuolization of the cytoplasm, balloon dystrophy. Conclusion. A comprehensive study of the structural components of the adenohypophysis of the test animals under the condition of a 30-day period of readaptation to long periods of consumption of heavy metal salts and correction with L-tocopherol certainly indicate a number of adaptive and regenerative morphofunctional changes aimed at reducing the stress of adaptive processes on the part of the adenohypophysis in response to cancel the action of the stress factor

scholarly journals Мorphofunctional Characteristics of the Male Reproductive System of White Rats Treated with Bisphenol A and Triclosan

2019 ◽  
Vol 8 (1) ◽  
pp. 77-81
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryukhin ◽  
S. N. Zav'yalov
Keyword(s):  
Bisphenol A ◽  
Leydig Cells ◽  
Statistical Processing ◽  
Mature Male ◽  
Male Rats ◽  
Total Count ◽  
White Rats ◽  
Comparison Groups ◽  
Negative Effect ◽  
Sexually Mature

The aimof this study was to analyze the morphofunctional state of the testes of mature male rats treated with bisphenol A (BPA) and triclosan (TCS).Material and methods.The work was performed on mature male rats (n=28). Experimental animals were divided into three groups – control (intact) and two experimental ones. For two months, rats of the experimental groups received daily bisphenol A and triclosan (Sigma-Aldrich, USA) with food in the amount of 200 mg/kg. The total count of spermatozoa in 1 ml of sperm was determined with the calculation of their atypical forms, and morphometric measurements were made (the total number and area of Leydig cells with their nuclearcytoplasmic ratio). The concentrations of bisphenol A and testicular triclosan were measured by gas chromatography with mass spectrometry (GC-MS). The obtained data was subjected to statistical processing using IBM SPSS Statistics v.21 (IBM Corp., Armonk, NY, USA).Results.Differences between the comparison groups in the concentration of bisphenol A and triclosan in testicular tissues were statistically significant (p <0.001). The endocrine disruptors studied reduced the mass of the testes. A group of male rats exposed to bisphenol A showed a decrease in the total number of spermatozoa (p=0.004) with an increase in their atypical forms (p=0.014) compared with a group of intact animals. Bisphenol A and triclosan caused a decrease in the total number of Leydig cells (p=0.001; p=0.001) respectively, and a statistically significant change in the nuclear-cytoplasmic ratio. Moreover, bisphenol A led to a decrease in the nuclear-cytoplasmic ratio of Leydig cells, whereas triclosal, on the contrary, increased its value in comparison with a group of intact animals.Conclusion.Bisphenol A and triclosan have a negative effect on the morphofunctional state of the male testes of sexually mature rats (decrease in testicular mass, total count of spermatozoa on the background of an increase in their atypical forms, as well as a decrease in the total number of Leydig cells with a change in their nuclear-cytoplasmic ratio).

scholarly journals COMPARATIVE CHARACTERISTICS OF THE MORPHOLOGICAL CHANGES OF NEPHRON TUBULES AT THE EARLY AND LATE STAGES OF THE CHRONIC OPIOID EFFECT

2019 ◽  
pp. 19-22
Author(s):  
Lesya Mateshuk-Vatseba ◽  
Iryna Vilkhova ◽  
Anna Bekesevych ◽  
Yeugen Paltov ◽  
Elena Kantser
Keyword(s):  
Morphological Changes ◽  
Mature Male ◽  
Male Rats ◽  
Histological Investigation ◽  
Early Stages ◽  
Hematoxylin And Eosin ◽  
Convoluted Tubules ◽  
Late Stages ◽  
Sexually Mature ◽  
Proximal Convoluted Tubules

The aim of the study is the examination of the nephron tubules morphological changes at the early and late stages of the experimental chronic opioid effect. Varying degrees of the morphological changes of proximal convoluted tubules, loops of Henle and distal convoluted tubules under chronic nalbuphine effect in the dynamics have been detected. The materials of the study – kidneys of 56 sexually mature male-rats. The experimental animals received daily intramuscular nalbuphine injections for 98 days. From the first to the fourteenth day of the experiment the dose of nalbuphine was 0,212 mg/kg. Every next two weeks nalbuphine injections were done in increasing doses. For histological investigation was used commonly agreed method. The colouring was performed using hematoxylin and eosin as well as azan by Heidenhain's method. Varying degrees of morphological changes of proximal convoluted tubules, loops of Henle and distal convoluted tubules under continuous nalbuphine injection in the dynamics have been detected. The most vivid alterative changes can be observed in proximal convoluted tubules both: at the early and late stages of the experiment. At the early stages as of the 14th day of the experiment signs of granular dystrophy appear, as of the 28th day – necrotic changes, progressing further at the late stages of the experiment. The loops of Henle undergo sporadic destructive changes at the early stages and at the beginning of the late stage of the experiment. Distal convoluted tubules have been necrotic altered only at the late stages of the experiment.

Dynamics of morphological and immunohistochemical changes in myocardium during hypothyroidism modeling in experiment

2022 ◽  
pp. 27-29
Author(s):  
V. S. Botasheva ◽  
N. A. Stadnik ◽  
A. B. Kubanova ◽  
N. I. Samoilov
Keyword(s):  
Immunohistochemical Study ◽  
Muscle Fibers ◽  
Mature Male ◽  
Male Rats ◽  
Laboratory Animals ◽  
Hydropic Degeneration ◽  
Standard Manner ◽  
Hematoxylin And Eosin ◽  
Left And Right ◽  
Sexually Mature

An experimental model of hypothyroidism was obtained by thyroidectomy. The operation was carried out on 57 sexually mature male rats weighing 250–300 g. The experiment lasted 45 days. Laboratory animals were removed from the experiment on days 7th, 14th, 21st, 28th, 35th and 45th. As a control, 15 rats were used that were not operated on. For histological examination, pieces of rat myocardium were taken from the left and right ventricles. The pieces were fixed in 10% buffered formalin for 10 days. Histological sections were prepared in a standard manner. Sections were stained with hematoxylin and eosin, picrofuchsin according to van Gieson, toluidine blue, according to Mallory. Results of the study: In the myocardium of rats, hemodynamic disorders, edema, hydropic degeneration of cardiomyocytes, myocytolysis, fragmentation of muscle fibers, colliquation necrosis, compression and atrophy of muscle fibers were found. An immunohistochemical study revealed a decrease in the expression of desmin and sarcomeric actin.

The first report of albinism in a Sundaland endemic rodent

Mammalia ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jonathan A. Nations ◽  
Ahmad Mursyid ◽  
Ryski Darma Busta ◽  
Sah Putra Adrian ◽  
Heru Handika ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Mature Male ◽  
Congenital Disorder ◽  
Domesticated Animals ◽  
First Report ◽  
West Sumatra ◽  
Sexually Mature

AbstractAlbinism, a congenital disorder that results in a lack of melanin deposition, is common in domesticated animals but rare in nature. Among the ∼2500 species of rodents worldwide, only 67 have published reports of albinism. Here we report the capture of an albino murid (Muridae: Rodentia) from Mt. Singgalang in West Sumatra, Indonesia. The specimen is an adolescent but sexually mature male Maxomys hylomyoides, a montane Sumatran endemic. To our knowledge, this specimen represents the first reported albino rodent from Indonesia and Sundaland, and only the second from Southeast Asia.

IMPACT OF EARLY LIFE STRESS ON ANTI-DEPRESSANT SENSITIVITY

2021 ◽  
pp. 123-132
Author(s):  
V.A. Vokina
Keyword(s):  
Open Field ◽  
Life Stress ◽  
Early Life ◽  
Early Life Stress ◽  
Anxiety Level ◽  
Male Rats ◽  
Porsolt Test ◽  
The Impact ◽  
Sexually Mature

Long-term consequences of impaired perinatal development are very significant. They appear during the neonatal period and in the first years of life, and persist during ontogenesis. There is little data on the impact of any prenatal factors on the sensitivity of a sexually mature organism to medications. The aim of the study is to assess the impact of early life stress on the development of individual antidepressant sensitivity. Materials and Methods. The authors conducted the experiments on sexually mature outbred male rats. To simulate the early life stress, a standard protocol was used. From the 2nd to 15th days of the postnatal period the pup rats were separated from their mother for 3 hours and kept in an incubator. The open-field test, Porsolt test and Sucrose consumption test were used to determine rat’s anxiety level as well as motor, orientation and exploratory activity at puberty. Then, for 14 days, the rats were intragastrically administered with a fluoxetine solution (10 mg/kg/daily), followed by their full examination. Statistical analysis of results was performed using the Mann-Whitney U-test to compare unrelated groups and Wilcoxon's test to compare related groups. Results. Fluoxetine did not have a pronounced antidepressant effect in animals that survived the early life stress. Such animals demonstrated passive floating during the Porsolt test, without any changes in immobility time. When testing in an open field, a sharp increase in the number of freezing behavior was observed, which was an indicator of an increased anxiety level in animals. Conclusion. The results obtained indicate that the long-term effects of neonatal stress may be associated with a change in antidepressant sensitivity or an increase in development of unwanted adverse reactions. Keywords: early life stress, depression, antidepressants, fluoxetine, rats. Отдаленные последствия нарушения перинатального развития весьма значительны и не только проявляются в период новорожденности и в первые годы жизни, но и сохраняются в период онтогенеза. Данные о влиянии каких-либо пренатальных факторов на чувствительность половозрелого организма к действию лекарственных веществ в доступной литературе представлены незначительно. Цель исследования – оценить роль стресса раннего периода жизни в формировании индивидуальной чувствительности к действию антидепрессантов. Материалы и методы. Эксперименты проведены на половозрелых беспородных крысах-самцах. Для моделирования стресса раннего периода жизни использовали стандартный протокол, подразумевающий отделение детенышей от матери со 2-го по 15-й дни постнатального периода на 3 ч в условиях инкубатора. В половозрелом возрасте проводили оценку уровня тревожности, двигательной и ориентировочно-исследовательской активности крыс в условиях теста открытого поля, теста Порсолта и теста «Потребление раствора сахарозы». Затем в течение 14 дней крысам внутрижелудочно вводили раствор флуоксетина (10 мг/кг/сут), после чего обследование повторяли в том же объеме. Статистический анализ результатов исследования проводили с использованием U-критерия Манна–Уитни для сравнения несвязанных групп и критерия Вилкоксона для сравнения связанных групп. Результаты. У животных, переживших стресс раннего периода жизни, флуоксетин не оказывал выраженного антидепрессантного действия. У данных животных в тесте Порсолта преобладало пассивное плавание, без изменения длительности иммобильности. При тестировании в открытом поле наблюдалось резкое повышение числа актов фризинга, что является показателем повышенного уровня тревожности у животных. Выводы. Полученные результаты свидетельствуют о том, что отдаленные последствия неонатального стресса могут быть связанны с изменением чувствительности к действию антидепрессантов или повышением риска развития нежелательных побочных реакций. Ключевые слова: стресс раннего периода жизни, депрессия, антидепрессанты, флуоксетин, крысы.

Exteroceptive Factors, Sexual Maturation and Reproduction in the Female Rat

1979 ◽  
Vol 13 (3) ◽  
pp. 283-286 ◽  
Author(s):  
M. L. Norris ◽  
C. E. Adams
Keyword(s):  
Reproductive Performance ◽  
Sexual Maturation ◽  
Sexual Maturity ◽  
Mature Male ◽  
Female Rat ◽  
Vaginal Opening ◽  
Sexually Mature

Summary Keeping a sexually mature male with a weanling female rat advanced neither the time of vaginal opening nor that of 1st oestrus. In 2 of 3 experiments females kept singly after weaning reached sexual maturity significantly earlier than did grouped females. The reproductive performance of females mated at 1st oestrus was not significantly different from that of older primiparae. 26 rats gave birth to an average of 9·3 young at 59·5 days of age, and 22 of them reared 96% of the young to weaning.

scholarly journals Immunohistochemical localization of melatonin in the rat Harderian gland.

1976 ◽  
Vol 24 (11) ◽  
pp. 1173-1177 ◽  
Author(s):  
G A Bubenik ◽  
G M Brown ◽  
L J Grota
Keyword(s):  
Acinar Cells ◽  
Harderian Gland ◽  
Immunohistochemical Localization ◽  
Mature Male ◽  
Male Rats ◽  
Secretory Cells ◽  
Yellow Fluorescence ◽  
Double Antibody

Using fluorescence and double antibody techniques, melatonin was localized immunohistologically in the secretory cells of the Harderian gland of mature male rats. The presence and quantity of melatonin in the acinar cells seem to correlate with the amount of porphyrins inside the lumen. The specificity was proven by disappearance of yellow fluorescence after saturation of antibody with melatonin or after use of nonspecific antibody only.

Medullary bone in male budgerigars (Melopsittacus undulatus) with testicular neoplasms

2022 ◽  
pp. 030098582110691
Author(s):  
Nathan K. Hoggard ◽  
Linden E. Craig
Keyword(s):  
Bone Density ◽  
Interstitial Cell ◽  
Egg Laying ◽  
Cell Tumor ◽  
Mature Male ◽  
Testicular Neoplasms ◽  
Testicular Neoplasm ◽  
Medullary Bone ◽  
Sustentacular Cell ◽  
Sexually Mature

Medullary bone is a calcium-rich, labile bone normally occurring in female birds with each egg-laying cycle. The stimulus for formation of medullary bone is, in part, the cyclic increase in serum estrogens produced by preovulatory ovarian follicles. Increased bone density due to formation of medullary bone, particularly in pneumatic bones, has been termed polyostotic hyperostosis, even if physiologic. This study investigated the formation of medullary bone in nonpneumatic (femur) and pneumatic (humerus) bones in sexually mature male budgerigars submitted for autopsy. Of the 21 sexually mature male budgerigars submitted for autopsy, 7 (33%) had medullary bone in 1 or more bones examined. All 7 male budgerigars with medullary bone had a testicular neoplasm, which was morphologically consistent with a testicular sustentacular cell tumor, seminoma, or interstitial cell tumor. Medullary bone was not present in the 14 cases with other diseases. Medullary bone formation in pneumatic and nonpneumatic bones can occur in male budgerigars with testicular neoplasms. Radiographic increases in medullary bone density, particularly in the humerus, could provide antemortem indication of testicular neoplasia in male budgerigars.

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