Dynamics of morphological and immunohistochemical changes in myocardium during hypothyroidism modeling in experiment

2022 ◽  
pp. 27-29
Author(s):  
V. S. Botasheva ◽  
N. A. Stadnik ◽  
A. B. Kubanova ◽  
N. I. Samoilov

An experimental model of hypothyroidism was obtained by thyroidectomy. The operation was carried out on 57 sexually mature male rats weighing 250–300 g. The experiment lasted 45 days. Laboratory animals were removed from the experiment on days 7th, 14th, 21st, 28th, 35th and 45th. As a control, 15 rats were used that were not operated on. For histological examination, pieces of rat myocardium were taken from the left and right ventricles. The pieces were fixed in 10% buffered formalin for 10 days. Histological sections were prepared in a standard manner. Sections were stained with hematoxylin and eosin, picrofuchsin according to van Gieson, toluidine blue, according to Mallory. Results of the study: In the myocardium of rats, hemodynamic disorders, edema, hydropic degeneration of cardiomyocytes, myocytolysis, fragmentation of muscle fibers, colliquation necrosis, compression and atrophy of muscle fibers were found. An immunohistochemical study revealed a decrease in the expression of desmin and sarcomeric actin.

2019 ◽  
pp. 19-22
Author(s):  
Lesya Mateshuk-Vatseba ◽  
Iryna Vilkhova ◽  
Anna Bekesevych ◽  
Yeugen Paltov ◽  
Elena Kantser

The aim of the study is the examination of the nephron tubules morphological changes at the early and late stages of the experimental chronic opioid effect. Varying degrees of the morphological changes of proximal convoluted tubules, loops of Henle and distal convoluted tubules under chronic nalbuphine effect in the dynamics have been detected. The materials of the study – kidneys of 56 sexually mature male-rats. The experimental animals received daily intramuscular nalbuphine injections for 98 days. From the first to the fourteenth day of the experiment the dose of nalbuphine was 0,212 mg/kg. Every next two weeks nalbuphine injections were done in increasing doses. For histological investigation was used commonly agreed method. The colouring was performed using hematoxylin and eosin as well as azan by Heidenhain's method. Varying degrees of morphological changes of proximal convoluted tubules, loops of Henle and distal convoluted tubules under continuous nalbuphine injection in the dynamics have been detected. The most vivid alterative changes can be observed in proximal convoluted tubules both: at the early and late stages of the experiment. At the early stages as of the 14th day of the experiment signs of granular dystrophy appear, as of the 28th day – necrotic changes, progressing further at the late stages of the experiment. The loops of Henle undergo sporadic destructive changes at the early stages and at the beginning of the late stage of the experiment. Distal convoluted tubules have been necrotic altered only at the late stages of the experiment.


Andrologia ◽  
2008 ◽  
Vol 40 (1) ◽  
pp. 29-37 ◽  
Author(s):  
M. Aleem ◽  
V. Padwal ◽  
J. Choudhari ◽  
N. Balasinor ◽  
M. K. Gill-Sharma

2017 ◽  
Vol 8 (2) ◽  
pp. 118-123 ◽  
Author(s):  
S. L. Popel’

This article reports the study of histo-ultrastructural changes of different structural components of the direct muscle of the thigh of sexually mature male rats over a prolonged period of hypokinesia and subsequent application of physical loading of average aerobic power. Using a light optical, electron microscope (for the exposure of structural transformations of muscle components) and histochemical (for determination of activity of succinate dehydrogenase according to the Nahlas method to identify muscle fibers with different phenotypes) methods, we studied the structural manifestations of adaptation of muscle fibres under prolonged (240 day) hypokinesia and 15–30 episodes of physical loading of average aerobic power in the posthypokinetic period among 55 sexually mature rats. Under prolonged hypokinesis we primarily observed changes in the intramuscular network and morphometric changes in the blood vessels. These data closely correlate with the progression of changes of the subcellular components responsible for energetic and flexible balance of muscle fibres. We found that fast oxygen-glycolytic muscle fibers and their peripheral nervous apparatus are the most sensitive to prolonged hypokinesia. As a result of application of physical loading of average aerobic power, reparative regeneration is intensified, which substantially shortens the period of recovery of structural-functional properties of skeletal muscles in the conditions of hypokinetic disorders. Thus, in prolonged hypokinesia, changes primarily affect the sources of blood supply to skeletal muscles, with the secondary development of reverse processes in muscle fibers and peripheral nervous apparatus with certain morphometric signs. 


2011 ◽  
Vol 26 (4) ◽  
pp. 279-284 ◽  
Author(s):  
Igor Eduardo Caetano Farias ◽  
Pedro Henrique Alves de Morais ◽  
Leonardo de Castro Durães ◽  
Fabiana Pirani Carneiro ◽  
Paulo Gonçalves de Oliveira ◽  
...  

PURPOSE: To evaluate the effects of increased intraperitoneal pressure caused by carbon dioxide pneumoperitoneum on the hepatic and renal morphology of rats. METHODS: Fifty-four adult male rats were randomly divided into three groups (P, PP and C) after anesthesia: P - in 18 animals, pneumoperitoneum was established for 30 minutes immediately before laparotomy; PP - in 18 animals, pneumoperitoneum was established for 60 minutes divided into 30 immediately before laparotomy and 30 after abdominal closure; control group (C) - 18 animals underwent laparotomy without pneumoperitoneum induction. The pneumoperitoneum was maintained at a pressure of 5 mm Hg. Nine animals in each group were killed on the 3rd and 7th postoperative days, when kidney and liver samples were collected for morphological analysis. The liver specimens were stained with hematoxylin and eosin (HE), and the kidney specimens, with HE and von Kossa. Blinded examiners analyzed the slides. RESULTS: No changes in renal morphology were found. Liver samples showed histological signs of degeneration in animals in the pneumoperitoneum groups killed on the 7th postoperative day (p=0.029). CONCLUSION: The CO2 pneumoperitoneum did not affect renal morphology but caused hydropic degeneration in the liver of animals killed on the 7th postoperative day.


2021 ◽  
Vol 6 (6) ◽  
pp. 245-252
Author(s):  
N. B. Hryntsova ◽  
◽  
A. M. Romanyuk ◽  
O. O. Tymakova ◽  
D. R. Khrin

The purpose of the study was to study the morphofunctional rearrangements of the structural components of the adenohypophysis of sexually mature male rats under the condition of a 30-day period of readaptation to the long-term influence of salts of heavy metals (zinc, copper, iron, manganese, lead and chromium) and taking a corrector drug. Materials and methods. The experiment was carried out on 24 white sexually mature male rats, which were divided into 1 control and 1 experimental group. The experimental group consisted of rats that consumed ordinary drinking water for 30 days after 90 days of receiving water saturated with a combination of heavy metal salts: zinc (ZnSO4 7H2O) – 5 mg / l, copper (CuSO4 5H2O) mg / l, iron (FeSO4) – 10 mg / l, manganese (MnSO4 5H2O) – 0.1 mg / l, lead (Pb (NO3) 2) – 0.1 mg / l and chromium (K2Cr2O7) – 0.1 mg / l and L-tocopherol corrector. Results and discussion. With a 30-day period of readaptation to the action of a complex of salts of heavy metals, combined with the intake of a corrector drug L-tocopherol, morphological rearrangements of a nonspecific polymorphic nature developed in the structural components of the pituitary gland: compensatory hypertrophy and increased vascularization of the pituitary gland were observed, the state of rheological properties of the blood improved, the number of chromophiles increased and the number of chromophobes decreased, the processes of cyst formation disappeared, the stress from the adaptive processes in the glandulocytes of the adenohypophysis decreased, namely their secretory activity, the expression of Hsp90α receptors in the cytoplasm of adenocytes significantly increased. Negative rearrangements include an increased value of the fibrous component of the connective tissue of the intertrabecular spaces, increased collagenization of the wall of veins and capillaries, preservation of stromal edema, plethora of hemocapillaries, the presence of a small number of adenocytes with signs of vacuolization of the cytoplasm, balloon dystrophy. Conclusion. A comprehensive study of the structural components of the adenohypophysis of the test animals under the condition of a 30-day period of readaptation to long periods of consumption of heavy metal salts and correction with L-tocopherol certainly indicate a number of adaptive and regenerative morphofunctional changes aimed at reducing the stress of adaptive processes on the part of the adenohypophysis in response to cancel the action of the stress factor


2019 ◽  
Vol 8 (1) ◽  
pp. 77-81
Author(s):  
S. V. Chigrinets ◽  
G. V. Bryukhin ◽  
S. N. Zav'yalov

The aimof this study was to analyze the morphofunctional state of the testes of mature male rats treated with bisphenol A (BPA) and triclosan (TCS).Material and methods.The work was performed on mature male rats (n=28). Experimental animals were divided into three groups – control (intact) and two experimental ones. For two months, rats of the experimental groups received daily bisphenol A and triclosan (Sigma-Aldrich, USA) with food in the amount of 200 mg/kg. The total count of spermatozoa in 1 ml of sperm was determined with the calculation of their atypical forms, and morphometric measurements were made (the total number and area of Leydig cells with their nuclearcytoplasmic ratio). The concentrations of bisphenol A and testicular triclosan were measured by gas chromatography with mass spectrometry (GC-MS). The obtained data was subjected to statistical processing using IBM SPSS Statistics v.21 (IBM Corp., Armonk, NY, USA).Results.Differences between the comparison groups in the concentration of bisphenol A and triclosan in testicular tissues were statistically significant (p <0.001). The endocrine disruptors studied reduced the mass of the testes. A group of male rats exposed to bisphenol A showed a decrease in the total number of spermatozoa (p=0.004) with an increase in their atypical forms (p=0.014) compared with a group of intact animals. Bisphenol A and triclosan caused a decrease in the total number of Leydig cells (p=0.001; p=0.001) respectively, and a statistically significant change in the nuclear-cytoplasmic ratio. Moreover, bisphenol A led to a decrease in the nuclear-cytoplasmic ratio of Leydig cells, whereas triclosal, on the contrary, increased its value in comparison with a group of intact animals.Conclusion.Bisphenol A and triclosan have a negative effect on the morphofunctional state of the male testes of sexually mature rats (decrease in testicular mass, total count of spermatozoa on the background of an increase in their atypical forms, as well as a decrease in the total number of Leydig cells with a change in their nuclear-cytoplasmic ratio).


Mammalia ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jonathan A. Nations ◽  
Ahmad Mursyid ◽  
Ryski Darma Busta ◽  
Sah Putra Adrian ◽  
Heru Handika ◽  
...  

AbstractAlbinism, a congenital disorder that results in a lack of melanin deposition, is common in domesticated animals but rare in nature. Among the ∼2500 species of rodents worldwide, only 67 have published reports of albinism. Here we report the capture of an albino murid (Muridae: Rodentia) from Mt. Singgalang in West Sumatra, Indonesia. The specimen is an adolescent but sexually mature male Maxomys hylomyoides, a montane Sumatran endemic. To our knowledge, this specimen represents the first reported albino rodent from Indonesia and Sundaland, and only the second from Southeast Asia.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.


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