Therapeutic effects of cefpirome (HR 810) on experimental mixed infections with enterococcus faecalis and escherichia coli in mice

Infection ◽  
1990 ◽  
Vol 18 (3) ◽  
pp. 186-190 ◽  
Author(s):  
S. Arai ◽  
S. Hayashi
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Therapeutic Effects ◽  
Mixed Infections

scholarly journals Comparison of the bactericidal activities of piperacillin-tazobactam, ticarcillin-clavulanate, and ampicillin-sulbactam against clinical isolates of Bacteroides fragilis, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa.

1997 ◽  
Vol 41 (2) ◽  
pp. 435-439 ◽  
Author(s):  
M E Klepser ◽  
M N Marangos ◽  
Z Zhu ◽  
D P Nicolau ◽  
R Quintiliani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Bactericidal Activity ◽  
Clinical Isolates ◽  
Mixed Infections ◽  
Dosing Interval ◽  
Drug Concentrations ◽  
Bactericidal Activities ◽  
Dosing Intervals

Owing to the broad spectrum of activity afforded by beta-lactam-beta-lactamase inhibitor preparations, these agents are frequently selected as empiric therapy for the treatment of mixed infections such as intra-abdominal and diabetic foot infections, either alone or in combination with an aminoglycoside. Twelve healthy volunteers were enrolled in a randomized, open-label, four-way crossover trial comparing the bactericidal activities of piperacillin-tazobactam, ticarcillin-clavulanate, and ampicillin-sulbactam against microorganisms commonly isolated from mixed infections. Subjects received the following regimes: (i) 3.375 g of piperacillin-tazobactam intravenously (i.v.) every 6 h (q6h) (ii) 4.5 g of piperacillin-tazobactam i.v. q8h, (iii) 3.1 g of ticarcillin-clavulanate i.v. q6h, and (iv) 3.0 g of ampicillin-sulbactam i.v. q6h. Serum bactericidal titers were determined and used to calculate the duration of measurable bactericidal activity over the dosing interval of each of the regimens against two clinical isolates of Bacillus fragilis, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa. The percentage of the dosing interval over which drug concentrations in serum remained above the MIC for each organism was determined and compared with the observed duration of bactericidal activity was noted (r = 0.78; P < 0.001). All of the regimens demonstrated good activity against B. fragilis and E. coli. Against E. faecalis and P. aeruginosa, however, all of the regimens provided bactericidal activity for less than 50% of the respective dosing intervals. These data suggest that use of shorter dosing intervals or continuous-infusion regimens should be considered in combination with an aminoglycoside to improve the bactericidal profiles of these agents for E. faecalis and P. aeruginosa.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

scholarly journals Urinary Excretion and Bactericidal Activities of Gemifloxacin and Ofloxacin after a Single Oral Dose in Healthy Volunteers

2001 ◽  
Vol 45 (12) ◽  
pp. 3524-3530 ◽  
Author(s):  
Christoph K. Naber ◽  
Michaela Hammer ◽  
Martina Kinzig-Schippers ◽  
Christian Sauber ◽  
Fritz Sörgel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Urinary Tract ◽  
Urinary Excretion ◽  
Enterococcus Faecalis ◽  
Urinary Tract Infections ◽  
Parent Drug ◽  
Oral Dosage ◽  
Tract Infections ◽  
Cumulative Excretion

ABSTRACT In a randomized crossover study, 16 volunteers (8 men, 8 women) received single oral doses of 320 mg of gemifloxacin and 400 mg of ofloxacin on two separate occasions in the fasting state to assess the urinary excretion and urinary bactericidal titers (UBTs) at intervals for up to 144 h. Ofloxacin showed higher concentrations in urine compared with those of gemifloxacin. The median (range) cumulative excretion of gemifloxacin was 29.7% (8.4 to 48.7%) of the parent drug administered, and median (range) cumulative excretion of ofloxacin was 84.3% (46.5 to 95.2%) of the parent drug administered. The UBTs, i.e., the highest twofold dilutions (with antibiotic-free urine as the diluent) of urine that were still bactericidal, were determined for a reference strain and nine uropathogens for which the MICs of gemifloxacin and ofloxacin were as follows:Escherichia coli ATCC 25922, 0.016 and 0.06 μg/ml, respectively; Klebsiella pneumoniae, 0.03 and 0.06 μg/ml, respectively; Proteus mirabilis, 0.125 and 0.125 μg/ml, respectively; Escherichia coli, 0.06 and 0.5 μg/ml, respectively; Pseudomonas aeruginosa, 1 and 4 μg/ml, respectively; Staphylococcus aureus, 0.008 and 0.25 μg/ml, respectively; Enterococcus faecalis, 0.06 and 2 μg/ml, respectively;Staphylococcus aureus, 0.25 and 4 μg/ml, respectively;Enterococcus faecalis, 0.5 and 32 μg/ml, respectively; and Staphylococcus aureus, 2 and 32 μg/ml, respectively. Generally, the UBTs for gram-positive uropathogens were higher for gemifloxacin than for ofloxacin and the UBTs for gram-negative uropathogens were higher for ofloxacin than for gemifloxacin. According to the UBTs, ofloxacin-resistant uropathogens (MICs, ≥4 mg/liter) should also be considered gemifloxacin resistant. Although clinical trials have shown that gemifloxacin is effective for the treatment of uncomplicated urinary tract infections, whether an oral dosage of 320 mg of gemifloxacin once daily is also adequate for the treatment of complicated urinary tract infections has yet to be confirmed.

scholarly journals Atividade antibacteriana de extratos de folhas de Montrichardia linifera (Arruda) Schott (Araceae)

2015 ◽  
Vol 17 (4 suppl 3) ◽  
pp. 1142-1149 ◽  
Author(s):  
J.A.L MIRANDA ◽  
J.A. ROCHA ◽  
K.M. ARAÚJO ◽  
P.V. QUELEMES ◽  
S.J. MAYO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis

RESUMO O uso de plantas medicinais no tratamento de doenças é uma estratégia antiga utilizada por praticamente todas as populações do mundo, e, embora novos antibióticos tenham sido desenvolvidos para o controle de micro-organismos infecciosos, às vezes são ineficazes. Diversos extratos de plantas medicinais têm efeitos antimicrobianos, principalmente quando associados à antibióticos de uso clínico, representando alternativa terapêutica para doenças infecciosas. Montrichardia linifera, conhecida popularmente como aninga, é espécie macrófita, aquática emergente de hábito herbáceo, pertencente a família Araceae e ocorre em áreas alagáveis. A utilidade farmacológica desta espécie é diversificada tendo sido relatada como cicatrizante, antirreumático, antidiurético e expectorante. Devido à relevância no campo etnofarmacológico, ampla utilização na medicina popular e escassez de trabalhos relacionados à atividade antibacteriana desta espécie, objetivou-se com este trabalho avaliar a atividade antibacteriana de extratos alcoólicos de folhas de Montrichardia linifera, coletadas na margem do rio Igaraçu, Parnaíba-PI. O extrato foi testado em oito cepas de bactérias: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. Foram utilizadas as técnicas de verificação da formação de halos de inibição e determinação das concentrações inibitórias e bactericidas mínimas. Os testes antibacterianos evidenciaram como principais resultados que o extrato metanólico seco (EMS), extrato metanólico fresco (EMF), e o extrato etanólico seco (EES), apresentaram ação antibacteriana, enquanto o extrato etanólico fresco (EEF) não apresentou atividade para as bactérias testadas. O EMS foi o mais eficiente, inibindo o crescimento bacteriano na concentração de 200 μg/mL para E. faecalis, 400 μg/mL para S. aureus, 400 μg/mL para S. epidermidis e 2.000 μg/mL para P. aeruginosa. O EMF obteve CIM de 2.000 μg/mL para E. faecalis e EES obteve CIM de 250 μg/mL para E. faecalis. Os resultados demonstraram que M. linifera constitui fonte eficiente de compostos bioativos antibacterianos. Os estudos sobre as propriedades farmacológicas de plantas da família Araceae são escassos, e os resultados deste trabalho são pioneiros em relação a atividade antibacteriana desta espécie.

scholarly journals Efeito antimicrobiano do extrato de Cranberry sobre micro-organismos causadores de infecção urinária

2016 ◽  
Vol 11 (31) ◽  
pp. 113-122
Author(s):  
Carla Franco Porto Belmont Souza ◽  
Luiz Eduardo Souza da Silva Irineu ◽  
Renan Silva De Souza ◽  
Renato da Silva Teixeira ◽  
Ivina Sanches Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Enterococcus Faecium ◽  
Vaccinium Macrocarpon ◽  
Mueller Hinton

A resistência microbiana tem se mostrado um problema de proporções mundiais, causando estado de morbidade e mortalidade em diversos pacientes. Em vista disso, tem crescido a busca por métodos alternativos naturais de profilaxia. A investigação clínica sugere que o Extrato de Cranberry está entre as melhores propostas de prevenção natural. O Cranberry (Vaccinium macrocarpon) é um fruto que tem crescido comercialmente pelo sabor e propriedades benéficas à saúde. Dentre as formas comercializadas estão: o suco, o chá e as cápsulas contendo o extrato seco. A ação desta planta está relacionada ao tratamento de doenças do trato urinário, por possuir substâncias que inibem a adesão bacteriana ao epitélio do trato urinário, dificultando sua proliferação e reprodução. Dentre todas as infecções relacionadas à assistência a saúde, a Infecção do Trato Urinário é a mais frequentemente associada a procedimentos invasivos. Se não for tratada, pode resultar em complicações como pielonefrite aguda, bacteremia e pionefrose. Portanto, cranberry pode ser uma nova alternativa para o combate das infecções uroepiteliais, por ser um produto natural de preço acessível, e com formas de comercialização diversificada, ao contrário dos antimicrobianos convencionais, que por sua vez são caros e podem acabar causando resistência nos micro-organismos. Este trabalho teve como objetivo avaliar in vitro a atividade antimicrobiana do extrato de Cranberry, adquirido em farmácia de manipulação, sobre 8 micro-organismos isolados de infecções urinárias. As cepas utilizadas, adquiridas da coleção da FIOCRUZ, foram: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Serratia marscecens, Staphylococcus aureus, Enterococcus faecalis e Enterococcus faecium. No estudo, foram utilizados o caldo Mueller Hinton (MH), Extrato de Cranberry e as bactérias patogênicas. O ensaio foi realizado em triplicata, com o uso de um controle de crescimento dos micro-organismos e o experimento para avaliação do crescimento bacteriano na presença do extrato. A turbidez foi medida com o auxílio de um espectrofotômetro, no comprimento de onda de 600 nm, antes e após 24 horas de incubação à 37 ºC. O procedimento forneceu a Densidade Ótica, do qual possibilitou a identificação da inibição microbiana. Para análise estatística foi utilizado o Teste t de Student. O Extrato de Cranberry apresentou atividade antimicrobiana sobre as bactérias Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Serratia marscecens e Enterococcus faecalis (p < 0,05), confirmando seu efeito benéfico em infecções urinárias. No entanto, não teve efeito inibitório significativo sobre Pseudomonas aeruginosa, Proteus mirabilis e Enterococcus faecium (p > 0,05).

scholarly journals FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

2021 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Antonio Carlos Pereira de Menezes Filho ◽  
Matheus Vinícius Abadia Ventura ◽  
Carlos Frederico de Souza Castro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Floral Organ ◽  
Salt Formation ◽  
Bacterial Strains ◽  
Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

scholarly journals Η επίδραση τοπικών αντιμικροβιακών παραγόντων σε εγκαύματα στην παιδική ηλικία

2011 ◽  
Author(s):  
Φωτεινή Μαυρίδη
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Enterobacter Cloacae ◽  
Staph Aureus

Σκοπός της μελέτης. Σκοπός της μελέτης ήταν να συγκρίνει την ιωδιούχο ποβιδόνη 10% και τον σουλφαδιαζινικό άργυρο 1%, όσον αφορά το βαθμό επιθηλιοποίησης, την πρόληψη των επιμολύνσεων, τις οργανικές επιπτώσεις εφαρμογής τους, καθώς και την ένταση του πόνου κατά την εφαρμογή τους σε παιδιατρικούς εγκαυματικούς ασθενείς. Πληθυσμός και μέθοδος. Το δείγμα αποτελέσαν 48 παιδιά ηλικίας έως 24 μηνών που εισήχθησαν για έγκαυμα μερικού πάχους από επαφή με θερμική πηγή ή καυτά υγρά. Κάθε παιδί που εκπλήρωνε τα κριτήρια εισόδου κατανεμόταν με τη μέθοδο της τυχαίας δειγματοληψίας α) στην ομάδα που εφαρμοζόταν το πρωτόκολλο θεραπείας για την ιωδιούχο ποβιδόνη 10% (Betadine), ή β) στην ομάδα που εφαρμοζόταν το πρωτόκολλο θεραπείας για το σουλφαδιαζινικό άργυρο 1% (Flamazine). Κατά την εισαγωγή καταγράφονταν δημογραφικά στοιχεία που αφορούσαν τη βαρύτητα του εγκαύματος και τις συνθήκες του συμβάντος. Την ώρα εισαγωγής (0) καθώς και τις ημέρες 1, 4, 7, 10, 14, 21, 30 καθώς και στους 3 ½ και 6½ μήνες: α) πραγματοποιούταν επισκόπηση της εγκαυματικής επιφάνειας και καταγραφή χρησιμοποιώντας κλίμακα από το 1-5 από δύο ανεξάρτητους παρατηρητές όσον αφορά την επιθηλιοποίηση, μόλυνση και επούλωση του τραύματος και β) λαμβάνονταν εργαστηριακές εξετάσεις (γενική αίματος, βιοχημικές και θυρεοειδικές εξετάσεις, ανοσοσφαιρίνες και συμπλήρωμα, γενική ούρων, καλλιέργειες εγκαυματικής επιφάνειας). Σε κάθε αλλαγή τραύματος και 3 ώρες μετά αξιολογούταν η ένταση του πόνου του παιδιού με τη βοήθεια της κλίμακας μέτρησης πόνου POCIS από τον ερευνητή-νοσηλευτή. Αποτελέσματα. Η μέση ηλικία των συμμετεχόντων ήταν 17,3 μήνες (±5,8 μήνες). Στο 54,2% των παιδιών (Ν=26) χρησιμοποιήθηκε ως τοπική θεραπεία Flamazine και στο υπόλοιπο 45,8% Betadine (Ν=22). Τα κυριότερα μικρόβια που εντοπίστηκαν στις καλλιέργειες εγκαυματικής επιφάνειας και στις δύο ομάδες ήταν ο staph. aureus (8,6%), ο enterococcus faecalis (3,9%), streptococcus sp (1,2%), το enterobacter cloacae (1,2%) και η escherichia coli (1,2%) οι διαφορές όμως μεταξύ των δύο ομάδων δεν υπήρξαν στατιστικά σημαντικές (p=0,100). Όσον αφορά τις καλλιέργειες αίματος στο 40% των περιπτώσεων στην ομάδα της ιωδιούχου ποβιδόνης υπήρξαν θετικές, σε αντίθεση με την ομάδα του σουλφαδιαζινικού αργύρου όπου καμία δε βρέθηκε θετική. Δεν παρατηρήθηκε στατιστικά σημαντική διαφορά στις ημέρες νοσηλείας μεταξύ των δύο ομάδων (12±6,1 ημέρες για την ομάδα ιωδιούχου ποβιδόνης και 13,7±8,8 ημέρες για την ομάδα σουλφαδιαζινικού αργύρου, p=0,453). Οι παράμετροι «οίδημα», «εκκρίσεις», «δυσοσμία», «κνησμός», «ερυθρότητα» και «φυσαλίδες» βρέθηκαν να συσχετίζονται ανεξάρτητα μόνο με το βάθος του εγκαύματος (p<0,001). Όσον αφορά την παράμετρο «επιθηλιοποίηση» βρέθηκε ότι το βάθος του εγκαύματος μπορεί να μειώσει το ρυθμό επιθηλιοποίησης (p<0,001), ενώ οι παράμετροι «ποιότητα κοκκιώδους ιστού» και «ουλώδης ιστός» ουσιαστικά δε βρέθηκε να επηρεάζονται από το βάθος και την έκταση του εγκαύματος (p=0,651 και p=0,649 αντίστοιχα) . Επιπλέον, ο βαθμός επιθηλιοποίησης των παιδιών με επιπολής εγκαύματα ήταν σημαντικά χαμηλότερος στην ομάδα Betadine σε σύγκριση με την ομάδα Flamazine (p=0,022). Αντίθετα, ο βαθμός επιθηλιοποίησης των παιδιών με εν τω βάθει 2ου βαθμού εγκαύματα δεν διέφερε σημαντικά μεταξύ των 2 ομάδων (p=0,783). Δεν παρατηρήθηκε σημαντική διαφορά μεταξύ των δύο ομάδων στο σκορ έντασης του πόνου τόσο κατά τη διάρκεια όσο και μετά την εφαρμογή (p=0,230). Σχετικά με τα αποτελέσματα των μονομεταβλητών αναλύσεων έχοντας σαν εξαρτημένες μεταβλητές τις εργαστηριακές εξετάσεις και σαν ανεξάρτητη την ομάδα θεραπείας οι παράμετροι που βρέθηκαν να είναι σημαντικά διαφορετικές μεταξύ των δύο ομάδων ήταν τα λευκά αιμοσφαίρια (p<0,001), τα αιμοπετάλια (p=0,035), οι τρανσαμινάσες (p=0,01 και p< 0,001 αντίστοιχα) και η Τ4 (p=0,025). Αντιθέτως, το βάθος του εγκαύματος φαίνεται να παίζει ρόλο στις τιμές των λευκών (p=0,008) και των ερυθρών αιμοσφαιρίων (p=0,030). Όσον αφορά την πορεία των εργαστηριακών μετρήσεων συνολικά στο χρόνο υπήρξε μείωση των λευκών (p=0,002), η οποία όμως δεν ήταν σημαντικά διαφορετική μεταξύ των ομάδων (p>0,05). Όσον αφορά τις τιμές των τρανσαμινασών και της γGT συνολικά στο χρόνο υπάρχει σημαντική μεταβολή μόνο της SGΟT (p=0,050), η οποία όμως δεν είναι σημαντικά διαφορετική μεταξύ των ομάδων (p>0,05). Συνολικά στο χρόνο δεν παρατηρείται σημαντική μεταβολή των ολικών λευκωμάτων (p=0,203), όμως η μεταβολή τους βρέθηκε να είναι σημαντικά διαφορετική μεταξύ των ομάδων (p=0,047). Οι τιμές των Τ3, Τ4 δεν παρουσίασαν σημαντική μεταβολή κατά τη διάρκεια των μελετώμενων ημερών, με εξαίρεση την TSH της οποίας η μεταβολή στο χρόνο υπήρξε σημαντική (p=0,011) όχι όμως και μεταξύ των ομάδων (p>0,05). Στο χρόνο σημειώθηκε σημαντική μεταβολή του IgG (p=0,006) και του IgM (p=0,006), ενώ δεν παρατηρήθηκε σημαντική μεταβολή του IgA (p=0,178). Οι μεταβολές αυτές δε βρέθηκαν να είναι σημαντικά διαφορετικές μεταξύ των ομάδων (p>0,05). Τέλος, δε βρέθηκε σημαντική διαφορά στις γενικές εξετάσεις ούρων των παιδιών μεταξύ των δύο ομάδων. Επιπλέον οι τιμές ουρίας και κρεατινίνης του ορού δεν σημείωσαν σημαντική μεταβολή ούτε συνολικά κατά τη διάρκεια της μελέτης, ούτε μεταξύ των δύο ομάδων (p=0,369 και p=0,187 αντίστοιχα). Συμπεράσματα. Σε παιδιά με μέτριας βαρύτητας εγκαύματα, όπου η διάρκεια έκθεσης στους μελετώμενους παράγοντες είναι περιορισμένη, η εφαρμογή των μελετώμενων τοπικών αντιμικροβιακών ουσιών είναι ασφαλής. Η χρήση του σουλφαδιαζινικού αργύρου φαίνεται να υπερέχει όσον αφορά τις αντιμικροβιακές του ιδιότητες και το ρυθμό επιθηλιοποίησης του εγκαυματικού τραύματος σε παιδιατρικούς εγκαυματικούς ασθενείς.

Therapeutic Effects of High Molecular Weight Hyaluronic Acid in Severe Pseudomonas Aeruginosa Pneumonia in Ex Vivo Perfused Human Lungs

2021 ◽  
Author(s):  
Xiwen Zhang ◽  
Shinji Sugita ◽  
Airan Liu ◽  
Yoshifumi Naito ◽  
Wonjung Hwang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Pseudomonas Aeruginosa ◽  
Hyaluronic Acid ◽  
High Molecular Weight ◽  
Bacterial Pneumonia ◽  
Ex Vivo ◽  
Therapeutic Effects ◽  
Human Lungs ◽  
Protein Permeability

Introduction: We previously reported that extracellular vesicles (EVs) released during Escherichia coli bacterial pneumonia were inflammatory, and administration of high molecular weight hyaluronic acid (HMW HA) suppressed several indices of acute lung injury (ALI) from Escherichia coli pneumonia by binding to these inflammatory EVs. The current study was undertaken to study the therapeutic effects of HMW HA in ex vivo perfused human lungs injured with Pseudomonas aeruginosa (PA)103 bacterial pneumonia. Methods: For lungs with baseline alveolar fluid clearance (AFC)<10%/h, HMW HA 1 or 2 mg was injected intravenously after 1 h (N = 4-9), and EVs released during PA pneumonia were collected from the perfusate over 6 h. For lungs with baseline AFC>10%/h, HMW HA 2 mg was injected intravenously after 1 h (N = 6). In vitro experiments were conducted to evaluate the effects of HA on inflammation and bacterial phagocytosis. Results: For lungs with AFC<10%/h, administration of HMW HA intravenously significantly restored AFC and numerically decreased protein permeability and alveolar inflammation from PA103 pneumonia but had no effect on bacterial counts at 6 h. However, HMW HA improved bacterial phagocytosis by human monocytes and neutrophils and suppressed the inflammatory properties of EVs released during pneumonia on monocytes. For lungs with AFC>10%/h, administration of HMW HA intravenously improved AFC from PA103 pneumonia but had no significant effects on protein permeability, inflammation or bacterial counts. Discussion: In the presence of impaired alveolar epithelial transport capacity, administration of HMW HA improved the resolution of pulmonary edema from Pseudomonas PA103 bacterial pneumonia.

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