Selection and proliferation of rapid growing cell lines from embryo derived cell cultures of yew tree (Taxus cuspidata Sieb. et Zucc)

Two hepatoma cell cultures were examined as in vitro models to be used in genotoxicity and cytotoxicity tests without the addition of bioactivating enzymes. The MH1C1, and HTC hepatoma lines were used in this study to establish their sensitivity to a number of xenobiotics, namely, cyclophosphamide (CP), the classical positive control in bioactivation tests; benzaldehyde (BA), a short-chain aldehyde; and 4-hydroxynonenal (HNE), a major toxic end-product of the peroxidative degradation of cell membrane lipids. As a first approach, we compared the following cytotoxicity tests: release of lactate dehydrogenase (LDH), and colony formation efficiency (CF). Colony-forming cells were exposed to the drugs according to different procedures, before or after the anchorage phase. The leakage of LDH into the medium following exposure of both cell lines to HNE, CP and BA for up to 24 hours was found not to be a good index of cytotoxicity. A better indicator of cytotoxicity was CF, as evaluated by exposure of the cells 24 hours after seeding. The effects were detectable at very low concentrations, corresponding to 10, 90 and 100μM for HNE, CP and BA, respectively. The impairment of CF efficiency was dose-dependent and time-dependent, and several differences between the two cell lines were observed.

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Differential heat shock response of primary human cell cultures and established cell lines

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(86)80332-1 ◽

1986 ◽

Vol 141 (1) ◽

pp. 46-52 ◽

Cited By ~ 11

Author(s):

Werner W. Richter ◽

Olaf-Georg Issinger

Keyword(s):

Heat Shock ◽

Cell Lines ◽

Cell Cultures ◽

Human Cell ◽

Heat Shock Response ◽

Differential Heat ◽

Established Cell Lines ◽

Human Cell Cultures ◽

Shock Response ◽

Established Cell

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Retroviral interleukin 5 gene transfer into interleukin 5-dependent growing cell lines results in autocrine growth and tumorigenicity

European Journal of Immunology ◽

10.1002/eji.1830201226 ◽

1990 ◽

Vol 20 (12) ◽

pp. 2699-2705 ◽

Cited By ~ 11

Author(s):

Thomas Blankenstein ◽

Weiqun Li ◽

Klaus Überla ◽

Zhihai Qin ◽

Akira Tominaga ◽

...

Keyword(s):

Gene Transfer ◽

Cell Lines ◽

Autocrine Growth ◽

Interleukin 5 ◽

Growing Cell

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Growth of Nosema cuniculi in established cell lines

Laboratory Animals ◽

10.1258/002367775780994835 ◽

1975 ◽

Vol 9 (1) ◽

pp. 61-68 ◽

Cited By ~ 9

Author(s):

T. Waller

Keyword(s):

Cell Lines ◽

Cell Cultures ◽

Growth Patterns ◽

Rabbit Kidney ◽

Kidney Cells ◽

Scale Production ◽

Simple Method ◽

Established Cell Lines ◽

Established Cell ◽

Canine Kidney

Growth patterns of Nosema cuniculi ( Encephalitozoon cuniculi) in cell cultures of bovine kidney, canine kidney, feline lung, and rabbit kidney were studied. All cell cultures used were easy to manage and the last 3 are commercially-available established cell lines. The dog kidney cells were the most suitable for large-scale production of Nosema. When grown in plastic flasks with a bottom area of 75 cm2, the weekly yield from Nosema-infected canine kidney cells during the 10th to 17th week after inoculation was between 4·1 x 107 and 9·9 x 107 spores per flask. An equilibrium was obtained between the Nosema infection and the kidney cells during this time. A simple method for estimating the numbel of harvested spores is also described.

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Antimigratory effect of berberine in T98G, U87MG and primary glioma cell culture.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e15045 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e15045-e15045

Author(s):

Irina V. Mezhevova ◽

Svetlana Yu. Filippova ◽

Sofia V. Timofeeva ◽

Anastasia O. Sitkovskaya ◽

Tatiana V. Shamova ◽

...

Keyword(s):

Cell Culture ◽

Cell Lines ◽

Cell Cultures ◽

Glioma Cell ◽

Glioma Cells ◽

Surgical Removal ◽

Experimental Sample ◽

Wound Area ◽

Migratory Activity ◽

Primary Glioma

e15045 Background: Berberine is an alkaloid compound with a structure that is highly similar to that of intercalating agents. It affects numerous cell signaling pathways and is widely studied as potential anticancer drug. It is known that berberine affects cancer cells migration through metalloproteinase-2 inhibition, but this effect was never studied on glioma cells. Anti-migratory drugs are of special interest in brain cancer therapy since glioma's highly invasive nature makes total surgical removal of tumor practically impossible. The aim of the study was to evaluate berberine anti-migratory activity on glioma cells. Methods: Cell migration capacity of T98G and U87MG cell lines, as well as primary glioma cell culture established in our laboratory, was assessed via standard wound healing assay with automated image acquisition and analysis on Lionheart FX (BioTek) cell imager. Prior to assay setting up cell cultures were maintained in DMEM medium with L-glutamine (1 μM) (Gibco) and 10% FBS (Gibco) at 37C0 and 5.0% CO2. Cells were seeded at 250 000 cells per well on 24-well plates and incubated overnight in order to attach to plate bottom. After that a vertical wound was made manually in each well, and berberine was added to experimental wells to final concentration 50 mg/L. Plates with cells were continuously incubated and photographed in cell imager at 37C0 and 5.0% CO2. The extent of cells migration was measured as the percent of wound area decrease after 24 hours of incubation in relation to starting time point. Data are given as: Mean ± 95% confidence interval. Results: In our study we berberine exhibited anti-migratory activity in all cell cultures under study. In rather fast growing primary cell culture wound area decrease was 99.23%±0.62% in control sample and 91.75%±0.28% in experimental sample. The difference was small but significant at p < 0.001 level (df = 30). Popular permanent glioma cell lines T98G and U87MG showed more prominent decrease in studied parameter with higher degree of variance at the same time. In T98G wound area decrease was 71.6%±12.3% in control and 48.8%± 7.6% in experimental samples after 24 hours of cultivation in presence of 50 mg/L berberine. While U87MG demonstrated 60.28%±5.13% and 37.5%± 8.34% wound area decrease accordingly. The obtained difference between control and experimental groups in permanent cell cultures was statistically significant at the 0.05 level (df = 30). Conclusions: Our preliminary research proved berberine to be potent anti-migratory agent in glioma treatment. Further investigations are needed to evaluate its ability to inhibit glioma cell expansion in vivo.

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Non-neuronal cell cultures from dorsal root ganglia of the adult cat: production of schwann-like cell lines

Brain Research ◽

10.1016/0006-8993(81)90753-8 ◽

1981 ◽

Vol 229 (1) ◽

pp. 163-181 ◽

Cited By ~ 13

Author(s):

Jean R. Wrathall ◽

Donald D. Rigamonti ◽

Mark R. Braford ◽

Carl C. Kao

Keyword(s):

Cell Lines ◽

Cell Cultures ◽

Dorsal Root Ganglia ◽

Dorsal Root ◽

Neuronal Cell ◽

Neuronal Cell Cultures ◽

Adult Cat

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HL-T, a new cell line derived from HL-60 promyelocytic leukemia cell cultures expressing terminal transferase and secreting suppressor activity

Blood ◽

10.1182/blood.v70.4.1151.bloodjournal7041151 ◽

1987 ◽

Vol 70 (4) ◽

pp. 1151-1160 ◽

Cited By ~ 1

Author(s):

E Paietta ◽

RJ Stockert ◽

T Calvelli ◽

P Papenhausen ◽

SV Seremetis ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Cell Line ◽

Cell Lines ◽

Cell Cultures ◽

Germ Line ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Chromosome 9 ◽

Terminal Transferase

A cell line with immature blast cell morphology was isolated from HL-60 promyelocytic leukemia cell cultures and designated HL-T. This new cell type is biphenotypic, expressing terminal transferase (TdT) together with myelomonocytoid immunologic features. TdT enzymatic activity, undetectable in HL-60, was determined to be 140 to 180 units/10(8) HL-T cells by the dGTP-assay, approximately 20% of the activity found in lymphoblastoid cell lines. HL-T predominantly synthesize the known 58- kDa TdT-protein plus a minor 54/56-kDa doublet. The 58-kDa steady state form is nonglycosylated and is phosphorylated. Precursor antigens S3.13 and MY-10, absent on HL-60, are expressed by HL-T; however, the cells are negative for HLA-Dr. Southern blot analysis by hybridization with immunoglobulin heavy chain (JH) and T cell-receptor chain gene (T beta) probes shows JH to be in the germ-line configuration in both cell lines and the T beta gene to be in germ-line in HL-60 but to be rearranged in HL-T. Truncation of the gene encoding the granulocyte-macrophage-colony- stimulating factor (GM-CSF), as found in HL-60, is not observed in HL- T. HL-T are resistant to differentiation-induction by retinoic acid and 1,25-dihydroxyvitamin D3. Cytogenetically HL-T share with HL-60 a deletion of the short arm of chromosome 9 at breakpoint p13, an aberration frequently found in patients with T cell leukemia. In addition, HL-T display t(8;9)(p11;p24) and trisomy 20. Tetraploidy is observed in 80% of HL-T metaphases with aberrations identical to those in the diploid karyotype. Like HL-60, the new line shows some surface- antigenic-T cell characteristics. Despite an antigenic pattern most consistent with that of helper-inducer T cells (T4+, D44+/-, 4B4+, 2H4- , TQ1+/-), HL-T cells and their conditioned culture medium suppress antigen, mitogen, and mixed-leukocyte-culture-mediated lymphocyte proliferation.

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Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines

F1000Research ◽

10.12688/f1000research.20135.2 ◽

2020 ◽

Vol 8 ◽

pp. 1597

Author(s):

Adriana E. Kajon ◽

Xiaoxin Li ◽

Gabriel Gonzalez ◽

Susan Core ◽

Helga Hofmann-Sieber ◽

...

Keyword(s):

Guinea Pig ◽

Cell Lines ◽

Cell Cultures ◽

Sequence Data ◽

Respiratory Illness ◽

Epithelial Cell Line ◽

Cavia Porcellus ◽

Diagnostic Assays ◽

Tracheal Epithelial

Background: The lack of adequate in vitro systems to isolate and propagate guinea pig adenovirus (GPAdV), a prevalent cause of respiratory illness of varaible severity in laboratory guinea pig colonies worldwide, has precluded its formal characterization to allow for the development of comprehensive diagnostic assays, and for the execution of complex pathogenesis and basic virology studies. Methods: Two strains of GPAdV were isolated in guinea pig (Cavia porcellus) cell cultures from frozen archival infected animal tissue originated from colony outbreaks of pneumonia in Australia and the Czech Republic in 1996. Results: Commercially available guinea pig cell lines from colorectal carcinoma (GPC-16), fetal fibroblast (104-C1) and lung fibroblast (JH4 C1), and the tracheal epithelial cell line GPTEC-T developed in this study were able to support viral infection and early propagation. Sufficient viral DNA was recovered from cell cultures to PCR-amplify and obtain sequence data for the complete hexon gene and partial DNA polymerase and penton base genes. Phylogenetic analysis for the three regions of the genome provided strong evidence confirming GPAdV as a unique species in the genus Mastadenovirus. Conclusions: This study demonstrated the feasibility of propagating GPAdV in cultures of immortalized lines of GP cells of a variety of types, thus establishing a critical foundation for the development of a robust culture platform for virus stock production and titration. The generation and analysis of whole GPAdV genome sequences will provide additional data for a comprehensive description of the genetic organization of the viral genome and for a better assessment of genetic diversity between the two isolated strains.

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CONTINUOUS CULTIVATION AND SUSCEPTIBILITY TO BOVINE VIRUSES OF CELL LINES DERIVED FROM BOVINE EMBRYONIC TISSUES

Canadian Journal of Microbiology ◽

10.1139/m58-052 ◽

1958 ◽

Vol 4 (5) ◽

pp. 487-492 ◽

Cited By ~ 4

Author(s):

Andrew S. Greig

Keyword(s):

Cell Lines ◽

Continuous Cultivation ◽

Bovine Embryo ◽

Virus Growth ◽

Skin Cells ◽

Culture Cells ◽

Growing Cell ◽

Growth Habits ◽

Bovine Viruses

Two cell lines, one derived from bovine embryo kidney, the other from bovine embryo skin, have been established in continuous in vitro culture. The cells of each line are predominantly of epithelial type in young cultures and become more spindle-shaped in older cultures. The kidney cells have progressed through 45 passages without change in morphology or growth habits, but the skin cells experienced a period of growth recession between the third and seventh passages, followed by the emergence of a new rapidly growing cell type. Each line of cells was shown to support the growth of the virus of infectious bovine rhinotracheitis and the agent of coital exanthema of cattle. In each case virus growth was accompanied by degenerative changes leading to death of the culture cells.

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