Trehalose synthesis in Saccharomycopsis fibuligera does not respond to stress treatments

2007 ◽  
Vol 74 (5) ◽  
pp. 1084-1091 ◽  
Author(s):  
L. K. Liang ◽  
X. K. Wang ◽  
K. L. Zhu ◽  
Z. M. Chi
Keyword(s):  
Saccharomycopsis Fibuligera ◽  
Trehalose Synthesis

scholarly journals Correlation of Intracellular Trehalose Concentration with Desiccation Resistance of Soil Escherichia coli Populations

2012 ◽  
Vol 78 (20) ◽  
pp. 7407-7413 ◽  
Author(s):  
Qian Zhang ◽  
Tao Yan
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
Potassium Acetate ◽  
Desiccation Stress ◽  
Desiccation Resistance ◽  
Content Type ◽  
Soil Desiccation ◽  
E Coli ◽  
Organic Solute ◽  
Trehalose Synthesis

ABSTRACTNaturalized soilEscherichia colipopulations need to resist common soil desiccation stress in order to inhabit soil environments. In this study, four representative soilE. colistrains and one lab strain, MG1655, were tested for desiccation resistance via die-off experiments in sterile quartz sand under a potassium acetate-induced desiccation condition. The desiccation stress caused significantly lower die-off rates of the four soil strains (0.17 to 0.40 day−1) than that of MG1655 (0.85 day−1). Cellular responses, including extracellular polymeric substance (EPS) production, exogenous glycine betaine (GB) uptake, and intracellular compatible organic solute synthesis, were quantified and compared under the desiccation and hydrated control conditions. GB uptake appeared not to be a specific desiccation response, while EPS production showed considerable variability among theE. colistrains. AllE. colistrains produced more intracellular trehalose, proline, and glutamine under the desiccation condition than the hydrated control, and only the trehalose concentration exhibited a significant correlation with the desiccation-contributed die-off coefficients (Spearman's ρ = −1.0;P= 0.02).De novotrehalose synthesis was further determined for 15E. colistrains from both soil and nonsoil sources to determine its prevalence as a specific desiccation response. MostE. colistrains (14/15) synthesized significantly more trehalose under the desiccation condition, and the soilE. colistrains produced more trehalose (106.5 ± 44.9 μmol/mg of protein [mean ± standard deviation]) than the nonsoil reference strains (32.5 ± 10.5 μmol/mg of protein).

Biochemical characterization of a glucoamylase from Saccharomycopsis fibuligera R64

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Dessy Natalia ◽  
Keni Vidilaseris ◽  
Pasjan Satrimafitrah ◽  
Wangsa Ismaya ◽  
Purkan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequence Identity ◽  
Biochemical Characterization ◽  
Saccharomycopsis Fibuligera ◽  
Sequence Identity ◽  
Ic50 Value ◽  
High Amino Acid ◽  
Ph Range

AbstractGlucoamylase from the yeast Saccharomycopsis fibuligera R64 (GLL1) has successfully been purified and characterized. The molecular mass of the enzyme was 56,583 Da as determined by mass spectrometry. The purified enzyme demonstrated optimum activity in the pH range of 5.6–6.4 and at 50°C. The activity of the enzyme was inhibited by acarbose with the IC50 value of 5 μM. GLL1 shares high amino acid sequence identity with GLU1 and GLA1, which are Saccharomycopsis fibuligera glucoamylases from the strains HUT7212 and KZ, respectively. The properties of GLL1, however, resemble that of GLU1. The elucidation of the primary structure of GLL1 contributes to the explanation of this finding.

Expression of Candidate Cold Stress and Metabolic Related Genes in Acidithiobacillus ferrivorans PQ33 Strain Using Ferrous Iron as Electron Donor

2017 ◽  
Vol 262 ◽  
pp. 368-371
Author(s):  
Gregory Guerra-Bieberach ◽  
Robert Ccorahua-Santo ◽  
Anika Eca ◽  
Jordan Bernaldo ◽  
Tito Sánchez ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Low Temperatures ◽  
Biofilm Formation ◽  
Iron Oxidation ◽  
Trehalose Synthase ◽  
Adaptation Mechanisms ◽  
Diguanylate Cyclases ◽  
Cold Adaptations ◽  
Trehalose Synthesis ◽  
Andean Mining

The identification of genes involved in cold adaptations of psychrotolerant bacteria Acidithiobacillus ferrivorans is important for biomining processes that take place at low temperatures like Andean mining installations in Peru. We have performed relative quantification RT-qPCR on candidate genes to have a role in adaptations at low temperature (5°C). The candidate genes analyzed were six: Two trehalose synthesis pathway genes, trehalose synthase (treS) and malto-oligosiltrehalose trehalohydrolase (treZ) showing no overexpression at 5°C. Two diguanylate cyclases genes related to exopolymer synthesis and biofilm formation (designated as dgc-I and dgc-II in this paper) were overexpressed at 21°C. The rusA and rusB genes involved in iron oxidation showed no significant change for rusA and no expression for rusB gene in any of both conditions. Genes rpoC, gyrB and alaS were validated as reference genes. These results show congruency with trancriptomics studies about gene expression of A. ferrivorans. Furthermore, the trehalose synthesis genes show no overexpression at low temperatures suggesting that other cold adaptation mechanisms are involved.

scholarly journals Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions

2020 ◽  
Vol 21 (2) ◽  
pp. 486 ◽  
Author(s):  
Zhuoheng Zhong ◽  
Takashi Furuya ◽  
Kimitaka Ueno ◽  
Hisateru Yamaguchi ◽  
Keisuke Hitachi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Proteomic Analysis ◽  
Millimeter Waves ◽  
Sugar Metabolism ◽  
Immunoblot Analysis ◽  
Glutathione Metabolism ◽  
Soybean Seeds ◽  
Soybean Seedlings ◽  
Promotive Effect ◽  
Trehalose Synthesis

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.

scholarly journals Trehalose recycling promotes energy-efficient mycomembrane reorganization in nutrient-limited mycobacteria

2019 ◽  
Author(s):  
Amol Arunrao Pohane ◽  
Caleb R. Carr ◽  
Jaishree Garhyan ◽  
Benjamin M. Swarts ◽  
M. Sloan Siegrist
Keyword(s):  
Energy Efficient ◽  
De Novo ◽  
Cell Envelope ◽  
Bacterial Pathogens ◽  
Atp Depletion ◽  
Redox Stress ◽  
Mycobacterial Cell ◽  
Resource Limited ◽  
Trehalose Synthesis ◽  
Long Chain

AbstractThe mycomembrane layer of the mycobacterial cell envelope is a barrier to environmental, immune and antibiotic insults. We find that there is mycomembrane remodeling along the periphery of nutrient-starved, non-replicating mycobacterial cells. Remodeling is supported by recycling of trehalose, a non-mammalian disaccharide that shuttles long-chain mycolate lipids to the mycomembrane. In the absence of trehalose recycling, mycomembrane synthesis continues but mycobacteria experience ATP depletion, enhanced respiration and redox stress. Redox stress from depletion of the trehalose pool is suppressed in a mutant that lacks the OtsAB de novo trehalose synthesis pathway. Our data suggest that trehalose recycling alleviates the energetic burden of mycomembrane remodeling. Loss of trehalose salvage is known to attenuate M. tuberculosis during infection and render the bacterium more susceptible to a variety of drugs. Recycling pathways are emerging targets for sensitizing resource-limited bacterial pathogens to host and antibiotic stress.

Trehalose Synthesis and Hydrolysis in Ascaris suum

1965 ◽  
Vol 51 (1) ◽  
pp. 76 ◽  
Author(s):  
C. F. Feist ◽  
C. P. Read ◽  
F. M. Fisher
Keyword(s):  
Ascaris Suum ◽  
Trehalose Synthesis
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