Metabolic changes in follicular fluids of patients treated with recombinant versus urinary human chorionic gonadotropin for triggering ovulation in assisted reproductive technologies: a metabolomics pilot study

2020 ◽  
Vol 302 (3) ◽  
pp. 741-751
Author(s):  
Maria Antonietta Castiglione Morelli ◽  
Assunta Iuliano ◽  
Sergio Crescenzo Antonio Schettini ◽  
Donatina Petruzzi ◽  
Angela Ferri ◽  
...  
Keyword(s):  
Pilot Study ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Metabolic Changes ◽  
Follicular Fluids

scholarly journals Effect of exogenous human chorionic gonadotropin on ovulation in mice

2020 ◽  
Vol 23 (8) ◽  
pp. 1006-1010
Author(s):  
S. Ya. Amstislavsky ◽  
S. V. Ranneva ◽  
D. S. Ragaeva ◽  
E. A. Chuyko ◽  
A. M. Popkova ◽  
...  
Keyword(s):  
Body Weight ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Polar Body ◽  
Reproductive Technologies ◽  
The Body ◽  
Hormonal Stimulation ◽  
Experimental Group

The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.

scholarly journals Relapse of Human Chorionic Gonadotropin-Induced Hyperthyroidism and Severe Hyperemesis Gravidarum Secondary to Twin-Twin Transfusion Syndrome, With Rapid Recovery Following Fetoscopic Laser Coagulation: Case Report

2021 ◽  
Vol 12 ◽  
Author(s):  
Faiza Lamine ◽  
Chiara Camponovo ◽  
David Baud ◽  
Dominique Werner ◽  
Laura Marino ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Twin Pregnancy ◽  
Assisted Reproductive Technologies ◽  
Hyperemesis Gravidarum ◽  
Reproductive Technologies ◽  
Laser Coagulation ◽  
Monochorionic Twin ◽  
Fetoscopic Laser Coagulation ◽  
Twin Pregnancies

BackgroundLimited data have shown that, compared to uncomplicated twin pregnancies, pregnancies complicated by twin-twin transfusion syndrome (TTTS), a life-threatening condition, are associated with higher maternal serum levels of both human chorionic gonadotropin (hCG) and thyroid hormones. With the continuing expansion of assisted reproductive technologies, the rate of twin pregnancies, including those complicated by TTTS and associated hyperemesis gravidarum, is expected to increase further. Therefore, detailed descriptions of the maternal and fetal clinical outcomes of maternal thyrotoxicosis linked to TTTS can be useful for timely diagnosis and management. However, such descriptions are currently lacking in the literature.Case PresentationWe report the case of a 30-year-old woman carrying a monochorionic twin pregnancy complicated by TTTS that induced a relapse of severe hyperemesis gravidarum with overt non-autoimmune hyperthyroidism at 17 weeks of gestation. Following fetoscopic laser coagulation (FLC), both hyperemesis and hyperthyroidism improved within 1 week.ConclusionsThe present experience contributes to the knowledge base on maternal thyrotoxicosis linked to TTTS and can be useful in the diagnosis and treatment of future cases; it also emphasizes the need for a high degree of clinical suspicion and for close collaboration between endocrinologists and obstetricians. Another key point is that TTTS-associated hyperemesis gravidarum and maternal hyperthyroidism should be considered in the differential diagnosis of refractory or relapsing hyperemesis gravidarum in women with monochorionic twin pregnancy, because this condition may require more stringent supportive treatment before and during the FLC procedure when the mother is overtly hyperthyroid.

Religious Diversity and Women’s Attitudes Toward Using Assisted Reproductive Technologies—Insights from a Pilot Study in Germany

2020 ◽  
Vol 7 (1) ◽  
pp. 150-168
Author(s):  
Nadja Milewski ◽  
Sonja Haug
Keyword(s):  
Pilot Study ◽  
Assisted Reproductive Technologies ◽  
Religious Affiliation ◽  
Religious Diversity ◽  
Muslim Women ◽  
Reproductive Technologies ◽  
High Rate ◽  
Social Relevance ◽  
Christian Women ◽  
The Social

Abstract This study examines women’s attitudes toward the own use of assisted reproductive technologies (ART) by their religious affiliation in Germany. The social relevance of ART is increasing in Western countries due to overall low birth rates, a high rate of childlessness, and a gap between the desired and the actual numbers of children. Previous literature has been scarce, however, on attitudes toward ART, and religious diversity has not been included in studies on ART. Our analysis is based on data collected in a pilot study in 2014 and 2015. The sample includes 944 women aged 18 to 50 living in Germany. The results show that Muslim women were significantly more likely than Christian women to say they would consider using ART; having no religious affiliation was associated with the least open attitude toward ART usage.

scholarly journals Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development

2021 ◽  
Vol 22 (22) ◽  
pp. 12422
Author(s):  
Chiara Di Berardino ◽  
Alessia Peserico ◽  
Giulia Capacchietti ◽  
Martina Crociati ◽  
Maurizio Monaci ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Oocyte Development ◽  
Cultural Conditions ◽  
Low Costs ◽  
Equine Chorionic Gonadotropin ◽  
Set Up ◽  
Positive Effect

The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (ivF) may represent a valid option to convey the large source of immature oocytes in ART. Several efforts have been made to set up ivF cultural protocols in medium-sized mammals, starting with the identification of the most suitable gonadotropic stimulus. In this study, Equine Chorionic Gonadotropin (eCG) is proposed as an alternative to Follicle Stimulating Hormone (FSH) based on its long superovulation use, trans-species validation, long half-life, and low costs. The use of 3D ivF on single-ovine preantral (PA) follicles allowed us to compare the hormonal effects and to validate their influence under two different cultural conditions. The use of eCG helped to stimulate the in vitro growth of ovine PA follicles by maximizing its influence under FBS-free medium. Higher performance of follicular growth, antrum formation, steroidogenic activity and gap junction marker expression were recorded. In addition, eCG, promoted a positive effect on the germinal compartment, leading to a higher incidence of meiotic competent oocytes. These findings should help to widen the use of eCG to ivF as a valid and largely available hormonal support enabling a synchronized in vitro follicle and oocyte development.

Development of assisted reproductive technologies for Mus spretus†

2020 ◽  
Author(s):  
Ayumi Hasegawa ◽  
Keiji Mochida ◽  
Shogo Matoba ◽  
Kimiko Inoue ◽  
Daiki Hama ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Laboratory Mouse ◽  
Reproductive Technologies ◽  
Efficient Production ◽  
High Survival ◽  
Mus Spretus ◽  
High Genetic Diversity

Abstract The genus Mus consists of many species with high genetic diversity. However, only one species, Mus musculus (the laboratory mouse), is common in biomedical research. The unavailability of assisted reproductive technologies (ARTs) for other Mus species might be a major reason for their limited use in laboratories. Here, we devised ARTs for Mus spretus (the Algerian mouse), a commonly used wild-derived Mus species. We found that in vitro production of M. spretus embryos was difficult because of low efficacies of superovulation with equine chorionic gonadotropin or anti-inhibin serum (AIS) (5–8 oocytes per female) and a low fertilization rate following in vitro fertilization (IVF; 15.2%). The primary cause of this was the hardening of the zona pellucida but not the sperm’s fertilizing ability, as revealed by reciprocal IVF with laboratory mice. The largest number of embryos (16 per female) were obtained when females were injected with AIS followed by human chorionic gonadotropin and estradiol injections 24 h later, and then by natural mating. These in vivo-derived 2-cell embryos could be vitrified/warmed with a high survival rate (94%) using an ethylene glycol-based solution. Importantly, more than 60% of such embryos developed into healthy offspring following interspecific embryo transfer into (C57BL/6 × C3H) F1 female mice. Thus, we have devised practical ARTs for Mus spretus mice, enabling efficient production of embryos and animals, with safe laboratory preservation of their strains. In addition, we have demonstrated that interspecific embryo transfer is possible in murine rodents.

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