Effect of swimming exercise, insulin-associated or not, on inflammatory cytokines, apoptosis, and collagen in diabetic rat placentas

2022 ◽  
Author(s):  
Mayra Maria da Silva Pereira ◽  
Ismaela Maria Ferreira de Melo ◽  
Valeska Andrea Ático Braga ◽  
Álvaro Aguiar Coelho Teixeira ◽  
Valéria Wanderley-Teixeira
Keyword(s):  
Inflammatory Cytokines ◽  
Swimming Exercise ◽  
Diabetic Rat

Moderate swimming exercise and caffeine supplementation reduce the levels of inflammatory cytokines without causing oxidative stress in tissues of middle-aged rats

Amino Acids ◽  
2014 ◽  
Vol 46 (5) ◽  
pp. 1187-1195 ◽  
Author(s):  
José L. Cechella ◽  
Marlon R. Leite ◽  
Fernando Dobrachinski ◽  
Juliana T. da Rocha ◽  
Nelson R. Carvalho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Cytokines ◽  
Swimming Exercise ◽  
Aged Rats ◽  
Middle Aged

scholarly journals Therapeutic Potential of Tpl2 (Tumor Progression Locus 2) Inhibition on Diabetic Vasculopathy Through the Blockage of the Inflammasome Complex

2020 ◽  
Author(s):  
Wayne Huey-Herng Sheu ◽  
Keng-Hung Lin ◽  
Jun-Sing Wang ◽  
De-Wei Lai ◽  
Wen-Jane Lee ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Tumor Progression ◽  
Inflammatory Cytokines ◽  
Molecular Mechanisms ◽  
Therapeutic Potential ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Angiogenic Factors ◽  
Mass Spectrometry Analysis ◽  
Diabetic Rat

Objective: Diabetic retinopathy, one of retinal vasculopathy, is characterized by retinal inflammation, vascular leakage, blood-retinal barrier breakdown, and neovascularization. However, the molecular mechanisms that contribute to diabetic retinopathy progression remain unclear. Approach and Results: Tpl2 (tumor progression locus 2) is a protein kinase implicated in inflammation and pathological vascular angiogenesis. N Ɛ -carboxymethyllysine (CML) and inflammatory cytokines levels in human sera and in several diabetic murine models were detected by ELISA, whereas liquid chromatography–tandem mass spectrometry analysis was used for whole eye tissues. The CML and p-Tpl2 expressions on the human retinal pigment epithelium (RPE) cells were determined by immunofluorescence. Intravitreal injection of pharmacological inhibitor or NA (neutralizing antibody) was used in a diabetic rat model. Retinal leukostasis, optical coherence tomography, and H&E staining were used to observe pathological features. Sera of diabetic retinopathy patients had significantly increased CML levels that positively correlated with diabetic retinopathy severity and foveal thickness. CML and p-Tpl2 expressions also significantly increased in the RPE of both T1DM and T2DM diabetes animal models. Mechanistic studies on RPE revealed that CML-induced Tpl2 activation and NADPH oxidase, and inflammasome complex activation were all effectively attenuated by Tpl2 inhibition. Tpl2 inhibition by NA also effectively reduced inflammatory/angiogenic factors, retinal leukostasis in streptozotocin-induced diabetic rats, and RPE secretion of inflammatory cytokines. The attenuated release of angiogenic factors led to inhibited vascular abnormalities in the diabetic animal model. Conclusions: The inhibition of Tpl2 can block the inflammasome signaling pathway in RPE and has potential clinical and therapeutic implications in diabetes-associated retinal microvascular dysfunction.

scholarly journals Implication of glycogen synthase kinase 3 in diabetes-associated islet inflammation

2020 ◽  
Vol 244 (1) ◽  
pp. 133-148 ◽  
Author(s):  
Caterina Luana Pitasi ◽  
Junjun Liu ◽  
Blandine Gausserès ◽  
Gaëlle Pommier ◽  
Etienne Delangre ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Cytokines ◽  
Glycogen Synthase ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3 ◽  
Diabetic Rat ◽  
Gk Rats ◽  
Islet Inflammation ◽  
The Impact ◽  
Pro Inflammatory Cytokines

Islet inflammation is associated with defective β cell function and mass in type 2 diabetes (T2D). Glycogen synthase kinase 3 (GSK3) has been identified as an important regulator of inflammation in different diseased conditions. However, the role of GSK3 in islet inflammation in the context of diabetes remains unexplored. In this study, we investigated the direct implication of GSK3 in islet inflammation in vitro and tested the impact of GSK3 inhibition in vivo, on the reduction of islet inflammation, and the improvement of glucose metabolism in the Goto-Kakizaki (GK) rat, a spontaneous model of T2D. GK rats were chronically treated with infra-therapeutic doses of lithium, a widely used inhibitor of GSK3. We analyzed parameters of glucose homeostasis as well as islet inflammation and fibrosis in the endocrine pancreas. Ex vivo, we tested the impact of GSK3 inhibition on the autonomous inflammatory response of non-diabetic rat and human islets, exposed to a mix of pro-inflammatory cytokines to mimic an inflammatory environment. Treatment of young GK rats with lithium prevented the development of overt diabetes. Lithium treatment resulted in reduced expression of pro-inflammatory cytokines in the islets. It decreased islet fibrosis and partially restored the glucose-induced insulin secretion in GK rats. Studies in non-diabetic human and rat islets exposed to inflammatory environment revealed the direct implication of GSK3 in the islet autonomous inflammatory response. We show for the first time, the implication of GSK3 in islet inflammation and suggest this enzyme as a viable target to treat diabetes-associated inflammation.

Swimming Impacts on Pancreatic Inflammatory Cytokines, miR-146a and NF-кB Expression Levels in Type-2 Diabetic Rats

2020 ◽  
Vol 16 (8) ◽  
pp. 889-894 ◽  
Author(s):  
Mohammad Reza Alipour ◽  
Nasibeh Yousefzade ◽  
Fariba Mirzaei Bavil ◽  
Roya Naderi ◽  
Rafighe Ghiasi
Keyword(s):  
Inflammatory Cytokines ◽  
Diabetic Group ◽  
Male Rats ◽  
Swimming Exercise ◽  
Control Group ◽  
Tissue Samples ◽  
Expression Levels ◽  
Tnf Α ◽  
Type 2 Diabetic

Background: Obesity-induced chronic inflammation is a key component in the pathogenesis of insulin resistance and type-2 diabetes Objective: This study aimed to evaluate the effect of swimming exercise on pancreatic expression levels of inflammatory cytokines, miR-146a and NF-кB in type-2 diabetic male rats. Method: Twenty- eight male Wistar rats were divided into four groups: control (Con), exercise, diabetes and diabetic exercise (n = 7). Diabetes induction performed by the combination of high-fat diet (HFD, 4 weeks) and streptozotocin (35 mg/kg. ip). After induction of diabetes, the rats swam in the exercise groups for 12 weeks. Then, blood and tissue samples were collected. Result: Our results indicated a significant increase in expression levels of miR-146, NF-κB and inflammatory cytokines (IL-6, TNF-α, and IL-1β) while a significant decrease in pancreatic expression levels of TRAF6 and IRAK1 in diabetic group as compared to the control group. In contrast, swimming exercise resulted in a significant decrease in expression levels of miR-146a, NF-кB and inflammatory cytokines and a significant increase in expression levels of TRAF6 and IRAK1 in the exercise-diabetic group compared to the diabetic group. Conclusion: Our results indicated a significant increase in expression levels of miR-146, NF-κB and inflammatory cytokines (IL-6, TNF-α, and IL-1β) while a significant decrease in pancreatic expression levels of TRAF6 and IRAK1 in diabetic group as compared to the control group. In contrast, swimming exercise resulted in a significant decrease in expression levels of miR-146a, NF-кB and inflammatory cytokines and a significant increase in expression levels of TRAF6 and IRAK1 in the exercise-diabetic group compared to the diabetic group.

scholarly journals Swimming Exercise Ameliorates Symptoms of MOG-Induced Experimental Autoimmune Encephalomyelitis by Inhibiting Inflammation and Demyelination in Rats

2020 ◽  
Vol 24 (Suppl 1) ◽  
pp. S39-47 ◽  
Author(s):  
Ji-Youn Kim ◽  
Eun-Surk Yi ◽  
Hyunju Lee ◽  
Jun-Su Kim ◽  
Yong-Seok Jee ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Weight Loss ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Motor Function ◽  
Inflammatory Cytokines ◽  
Swimming Exercise ◽  
Interferon Β ◽  
Autoimmune Encephalomyelitis ◽  
Experimental Autoimmune ◽  
Pro Inflammatory Cytokines

Purpose: Multiple sclerosis is an autoimmune disease that affects the central nerve system, resulting in cumulative loss of motor function. Multiple sclerosis is induced through multiple mechanisms and is caused by inflammation and demyelination. This study aims to evaluate the neuroprotective effect of swimming exercise in experimental autoimmune encephalomyelitis (EAE) rats, an animal model of multiple sclerosis.Methods: EAE was induced by an intradermal injection of 50-μg purified myelin oligodendrocyte glycoprotein 33–55 (MOG<sub>33-55</sub>) dissolved in 200-μL saline at the base of the tail. The rats in the swimming exercise group were made to swim for 30 minutes once pert a day for 26 consecutive days, starting 5 days after induction of EAE. To compare the effect of swimming exercise with interferon-β, a drug for multiple sclerosis, interferon-β was injected intraperitoneally into rats of the EAE-induced and interferon-β-treated group during the exercise period.Results: Injection of MOG<sub>33-55</sub> caused weight loss, decreased clinical disability score, and increased level of pro-inflammatory cytokines and inflammatory mediators in the lumbar spinal cord. Loss of motor function and weakness increased demyelination score. Swimming exercise suppressed demyelination and expression of pro-inflammatory cytokines and inflammatory mediators. These changes promoted recovery of EAE symptoms such as body weight loss, motor dysfunction, and weakness. Swimming exercise caused the same level of improvement as interferon-β treatment.Conclusions: The results of this experiment suggest the possibility of swimming exercise in urological diseases that are difficult to treat. Swimming exercises can be considered for relief of symptom in incurable multiple sclerosis.

Swimming exercise and diphenyl diselenide-supplemented diet affect the serum levels of pro- and anti-inflammatory cytokines differently depending on the age of rats

Cytokine ◽  
2015 ◽  
Vol 71 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Marlon R. Leite ◽  
José L. Cechella ◽  
Anderson C. Mantovani ◽  
Marta M.M.F. Duarte ◽  
Cristina W. Nogueira ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Serum Levels ◽  
Swimming Exercise ◽  
Diphenyl Diselenide ◽  
Anti Inflammatory

Cytokine synergy, collagenases and cartilage collagen breakdown

2003 ◽  
Vol 70 ◽  
pp. 125-133 ◽  
Author(s):  
Tim E. Cawston ◽  
Jenny M. Milner ◽  
Jon B. Catterall ◽  
Andrew D. Rowan
Keyword(s):  
Matrix Metalloproteinases ◽  
Inflammatory Cytokines ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
And Cartilage ◽  
Pro Inflammatory Cytokines

We have investigated proteinases that degrade cartilage collagen. We show that pro-inflammatory cytokines act synergistically with oncastatin M to promote cartilage collagen resorption by the up-regulation and activation of matrix metalloproteinases (MMPs). The precise mechanisms are not known, but involve the up-regulation of c-fos, which binds to MMP promoters at a proximal activator protein-1 (AP-1) site. This markedly up-regulates transcription and leads to higher levels of active MMP proteins.

Sign in / Sign up

Export Citation Format

Share Document