scholarly journals Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings

2021 ◽  
Author(s):  
Marek Widera ◽  
Sandra Westhaus ◽  
Holger F. Rabenau ◽  
Sebastian Hoehl ◽  
Denisa Bojkova ◽  
...  
Keyword(s):  
Culture Media ◽  
Vero Cells ◽  
Heat Inactivation ◽  
The Novel ◽  
Safety Issues ◽  
Rapid Spread ◽  
Level 3 ◽  
Infected Cells ◽  
Novel Coronavirus ◽  
Plastic Surfaces

AbstractThe novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Laboratory work with SARS-CoV-2 in a laboratory setting was rated to biosafety level 3 (BSL-3) biocontainment level. However, certain research applications in particular in molecular biology require incomplete denaturation of the proteins, which might cause safety issues handling contaminated samples. In this study, we evaluated lysis buffers that are commonly used in molecular biological laboratories for their ability to inactivate SARS-CoV-2. In addition, viral stability in cell culture media at 4 °C and on display glass and plastic surfaces used in laboratory environment was analyzed. Furthermore, we evaluated chemical and non-chemical inactivation methods including heat inactivation, UV-C light, addition of ethanol, acetone-methanol, and PFA, which might be used as a subsequent inactivation step in the case of insufficient inactivation. We infected susceptible Caco-2 and Vero cells with pre-treated SARS-CoV-2 and determined the tissue culture infection dose 50 (TCID50) using crystal violet staining and microscopy. In addition, lysates of infected cells and virus containing supernatant were subjected to RT-qPCR analysis. We have found that guanidine thiocyanate and most of the tested detergent containing lysis buffers were effective in inactivation of SARS-CoV-2, however, the M-PER lysis buffer containing a proprietary detergent failed to inactivate the virus. In conclusion, careful evaluation of the used inactivation methods is required especially for non-denaturing buffers. Additional inactivation steps might be necessary before removal of lysed viral samples from BSL-3.

Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings

2020 ◽  
Author(s):  
Sandra Westhaus ◽  
Marek Widera ◽  
Holger F. Rabenau ◽  
Sebastian Hoehl ◽  
Denisa Bojkova ◽  
...  
Keyword(s):  
Culture Media ◽  
Heat Inactivation ◽  
The Novel ◽  
Safety Issues ◽  
Rapid Spread ◽  
Level 3 ◽  
Global Concern ◽  
The Stability ◽  
Novel Coronavirus ◽  
Uv C

SummaryThe novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Laboratory work with SARS-CoV-2 in a laboratory setting was rated to biosafety level 3 (BSL-3) biocontainment level. However, certain research applications in particular in molecular biology require incomplete denaturation of the proteins, which might cause safety issues handling contaminated samples. In particular, it is critical to provide proof of inactivation before samples can be removed from the BSL-3.In this study, the stability of the virus in cell culture media at 4°C and on touch panel surfaces used in laboratory environment was analyzed. In addition, we evaluated common lysis buffers that are used in molecular biological laboratories for their ability to inactivate SARS-CoV-2. We have found that guanidine thiocyanate and most of the tested detergent containing lysis buffers were effective in inactivation of SARS-CoV-2, however, the M-PER lysis buffer containing a proprietary detergent failed to inactivate SARS-CoV-2. Furthermore, we compared chemical and non-chemical inactivation methods including ethanol, acetone-methanol mixture, PFA, UV-C light, and heat inactivation.In conclusion, careful evaluation of the used inactivation methods are required and additional inactivation steps are necessary before removal of lysed viral samples from BSL-3.

scholarly journals Propagation, Inactivation, and Safety Testing of SARS-CoV-2

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 622 ◽  
Author(s):  
Alexander S. Jureka ◽  
Jesus A. Silvas ◽  
Christopher F. Basler
Keyword(s):  
Plaque Assay ◽  
Virus Infectivity ◽  
Safety Testing ◽  
Level 3 ◽  
Chinese Province ◽  
Infected Cells ◽  
Multiple Cell ◽  
Novel Coronavirus ◽  
Effective Procedures ◽  
Biosafety Level

In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandemic has instigated widespread research related to SARS-CoV-2 and the disease that it causes, COVID-19. Research into this new virus will be facilitated by the availability of clearly described and effective procedures that enable the propagation and quantification of infectious virus. As work with the virus is recommended to be performed at biosafety level 3, validated methods to effectively inactivate the virus to enable the safe study of RNA, DNA, and protein from infected cells are also needed. Here, we report methods used to grow SARS-CoV-2 in multiple cell lines and to measure virus infectivity by plaque assay using either agarose or microcrystalline cellulose as an overlay as well as a SARS-CoV-2 specific focus forming assay. We also demonstrate effective inactivation by TRIzol, 10% neutral buffered formalin, beta propiolactone, and heat.

scholarly journals The IMPDH inhibitor merimepodib provided in combination with the adenosine analogue remdesivir reduces SARS-CoV-2 replication to undetectable levels in vitro

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 361
Author(s):  
Natalya Bukreyeva ◽  
Rachel A. Sattler ◽  
Emily K. Mantlo ◽  
Timothy Wanninger ◽  
John T. Manning ◽  
...  
Keyword(s):  
Disease Burden ◽  
Vero Cells ◽  
The United States ◽  
The Novel ◽  
Infectious Titer ◽  
Adenosine Analogue ◽  
Pre Treatment ◽  
Novel Coronavirus ◽  
Dose Dependent

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the novel coronavirus responsible for the ongoing COVID-19 pandemic, which has resulted in over 2.5 million confirmed cases and 170,000 deaths worldwide as of late April 2020. The pandemic currently presents major public health and economic burdens worldwide. No vaccines or therapeutics have been approved for use to treat COVID-19 cases in the United States despite the growing disease burden, thus creating an urgent need for effective treatments. The adenosine analogue remdesivir (REM) has recently been investigated as a potential treatment option, and has shown some activity in limiting SARS-CoV-2 replication. We previously reported that the IMPDH inhibitor merimepodib (MMPD) provides a dose-dependent suppression of SARS-CoV-2 replication in vitro. Here, we report that a 4-hour pre-treatment of Vero cells with 2.5µM MMPD reduces the infectious titer of SARS-CoV-2 more effectively than REM at the same concentration. Additionally, pre-treatment of Vero cells with both REM and MMPD in combination reduces the infectious titer of SARS-CoV-2 to values below the detectable limit of our TCID50 assay. This result was achieved with concentrations as small as 1.25 µM MMPD and 2.5 µM REM. At concentrations of each agent as low as 0.31 µM, significant reduction of viral production occurred. This study provides evidence that REM and MMPD administered in combination might be an effective treatment for COVID-19 cases.

scholarly journals The effects of the clinical symptoms pneumonia-confirmation strategy of the COVID-19 epidemic in Wuhan, China

2020 ◽  
Author(s):  
Yanjin Wang ◽  
Pei Wang ◽  
Shudao Zhang ◽  
Hao Pan
Keyword(s):  
Turning Point ◽  
Clinical Symptoms ◽  
Transmission Rate ◽  
Basic Reproductive Number ◽  
Reproductive Number ◽  
The Novel ◽  
Wuhan City ◽  
Start Date ◽  
Rapid Spread ◽  
Novel Coronavirus

Abstract Motivated by the quick control in Wuhan, China, and the rapid spread in other countries of COVID-19, we investigate the questions that what is the turning point in Wuhan by quantifying the variety of basic reproductive number after the lockdown city. The answer may help the world to control the COVID-19 epidemic. A modified SEIR model is used to study the COVID-19 epidemic in Wuhan city. Our model is calibrated by the hospitalized cases. The modeling result gives out that the means of basic reproductive numbers are 1.5517 (95% CI 1.1716-4.4283) for the period from Jan 25 to Feb 11, 2020, and 0.4738(95% CI 0.0997-0.8370) for the period from Feb 12 to Mar 10. The transmission rate fell after Feb 12, 2020 as a result of China’s COVID-19 strategy of keeping society distance and the medical support from all China, but principally because of the clinical symptoms to be used for the novel coronavirus pneumonia (NCP) confirmation in Wuhan since Feb 12, 2020. Clinical diagnosis can quicken up NCP-confirmation such that the COVID-19 patients can be isolated without delay. So the clinical symptoms pneumonia-confirmation is the turning point of the COVID-19 battle of Wuhan. The measure of clinical symptoms pneumonia-confirmation in Wuhan has delayed the growth and reduced size of the COVID-19 epidemic, decreased the peak number of the hospitalized cases by 96% in Wuhan. Our modeling also indicates that the earliest start date of COVID-19 in Wuhan may be Nov 2, 2019.

scholarly journals Knowledge Hiding: One of the Primary Reasons behind the Rapid Spread of the Novel Coronavirus COVID-19

2021 ◽  
Vol 24 (2) ◽  
pp. 173-177
Author(s):  
Abraham Cyril Issac
Keyword(s):  
World Health ◽  
The Novel ◽  
Rapid Spread ◽  
The World ◽  
Human Tendency ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Cordon Sanitaire ◽  
Knowledge Hiding ◽  
Different Sources

Abstract The world is battling out the pandemic of Covid-19. The World Health Organization (WHO) is jointly acting upon the same daily, which is evident from the ‘situation reports.’ The pandemic, which saw its origin in Wuhan, has spread across the world within a short span of under two months. While the pandemic has effectively instilled a situation of cordon sanitaire across the globe, the virus seems to show no respite. This study collates different sources and establishes the human tendency of knowledge hiding as the prime reason for the spread of such colossal magnitudes. The study underlines the notion by examining some of the critical cases and situations that have unfolded in the very recent past.

scholarly journals Political Ideology and the Outbreak of COVID-19 in the United States

2020 ◽  
Author(s):  
Daniel L. Rosenfeld
Keyword(s):  
United States ◽  
Political Ideology ◽  
State Level ◽  
The United States ◽  
The State ◽  
The Novel ◽  
Unique Role ◽  
Rapid Spread ◽  
Novel Coronavirus

At the state level within the United States, did political ideology predict the outbreak of the novel coronavirus (COVID-19)? Throughout March 2020, the United States became the epicenter of the COVID-19 pandemic, recording the most cases of any country worldwide. The current research found that, at the state level within the United States, more conservative political ideology predicted delayed implementation of stay-at-home orders and more rapid spread of COVID-19. Effects were significant across two distinct operationalizations of political ideology and held over and above relevant covariates, suggesting a potentially unique role of political ideology in the United States’ COVID-19 outbreak. Considering political ideological factors may offer valuable insights into epidemiological processes surrounding COVID-19.

scholarly journals Analysis of the stochastic model for predicting the novel coronavirus disease

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
Ndolane Sene
Keyword(s):  
Stochastic Model ◽  
Deterministic Model ◽  
Reproduction Number ◽  
Equilibrium Points ◽  
The Novel ◽  
Stochastic Perturbations ◽  
Numerical Investigations ◽  
Rapid Spread ◽  
The World ◽  
Novel Coronavirus

Abstract In this paper, we propose a mathematical model to predict the novel coronavirus. Due to the rapid spread of the novel coronavirus disease in the world, we add to the deterministic model of the coronavirus the terms of the stochastic perturbations. In other words, we consider in this paper a stochastic model to predict the novel coronavirus. The equilibrium points of the deterministic model have been determined, and the reproduction number of our deterministic model has been implemented. The asymptotic behaviors of the solutions of the stochastic model around the equilibrium points have been studied. The numerical investigations and the graphical representations obtained with the novel stochastic model are made using the classical stochastic numerical scheme.

scholarly journals A comprehensive review on epidemiology, aetiopathogenesis, diagnosis and treatment of the novel coronavirus syndrome – COVID-19

2020 ◽  
Vol 2 (2) ◽  
pp. 110-123
Author(s):  
Kulvinder Kochar Kaur ◽  
◽  
Gautam Allahbadia ◽  
Mandeep Singh ◽  
◽  
...  
Keyword(s):  
Respiratory Illness ◽  
World Health ◽  
The Novel ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Rapid Spread ◽  
Polymerase Chain ◽  
Novel Coronavirus ◽  
Health Organization ◽  
Severe Respiratory Illness

Since the outbreak of the novel Coronavirus in December 2019 in Wuhan China, this novel Coronavirus disease(COVID-19) has spread worldwide taking not only epidemic proportions but with its rapid spread world health organization(WHO) was forced to declare it as a pandemic. The Severe Acute respiratory syndrome (SARS)-Coronavirus (CoV2) virus is responsible for clusters of severe respiratory illness that simulates acute respiratory syndrome that was what was initially. It is thought although that it is equivalent to the high altitude pulmonary oedema (HAPE), showing glass ground opacities in lungs. More experience is getting acquired with changes in treatment approaches from PEEP to avoid intubation and just ensure oxygen levels maintained. Human to human transmission through droplets, contaminated hands as well as surfaces, has been revealed with an incubation period varying from 2-14 days. Early diagnosis using reverse transcription polymerase chain reaction (RT-PCR) or computed tomography (CT) scan chest, quarantine, as well as supportive treatment are necessary for getting a cure. In this review we have tried to analyze the epidemiology, diagnosis, isolation, and treatment, including antiviral drugs like remdesivir, favipiravir, chloroquine and hydroxychloroquine, corticosteroids, antibiotics, and ivermectin. With 3 successful cases of convalescent plasma achieved in USA, trials going on in India along with vaccines are also detailed in this article.

scholarly journals Making Sense of Pandemic COVID-19 and its Affect(abilities) in the Indian Context

2021 ◽  
Vol 20 (1) ◽  
pp. 115-133
Author(s):  
Abhisek Ghosal
Keyword(s):  
The Novel ◽  
Self Monitoring ◽  
The Third ◽  
Making Sense ◽  
Indian Context ◽  
Rapid Spread ◽  
The Government ◽  
To Come ◽  
Novel Coronavirus ◽  
Restrictive Measures

This article seeks to reflect on pandemic COVID-19 and its diverse affect(abilities) in the context of India. After the outbreak of the novel coronavirus in India, the government resorted to a number of restrictive measures including quarantine, lockdown, self-isolation, and self-monitoring in order to contain the rapid spread of the new virus. This article argues that the pandemic has rendered “historical ruptures” to the world at large, and seeks to examine how it has affected the ‘usual’ ways of living of marginalised people in India, including how migrant labourers have had to strive to come to terms with the dreadful consequences of the pandemic. Furthermore, this article puts into focus how certain governmental measures are brought into effect to check the affect(abilities) of COVID-19. In order to elaborate on these affect(abilities) certain critical philosophical standpoints are drawn. In the opening section, bio-philosophical nuances of illness are expounded. These are followed, in the second section, by a discussion of neuroeconomical aspects of these affect(abilities). In the third section, theoretical notions of potentiality, singularity, and transpolitical becomings are examined through Continental philosophies. Finally, particularities of the Indian context are critically elucidated in the context of affect(abilities) of pandemic COVID-19.

scholarly journals Economic impact of COVID-19 on the Indian healthcare sector: an overview

2020 ◽  
Vol 8 (1) ◽  
pp. 489
Author(s):  
Divyanshu Shukla ◽  
Arvind Pradhan ◽  
Parul Malik
Keyword(s):  
Healthcare Sector ◽  
The Novel ◽  
Indian Government ◽  
Marginalized Communities ◽  
Financial Centre ◽  
Rapid Spread ◽  
Novel Coronavirus ◽  
Long Term Impact ◽  
Research Centres

A fearsome health calamity- the novel coronavirus of 2019 struck the world and created havoc with its rapid spread. Various countries got affected in terms of economic and other losses and the extent of impact depended on the pre-existing financial arrangement of the country and various other factors. Due to this outbreak, healthcare sector was one of the worst affected. Also, it needed most investment and had to respond quickly and effectively to manage this crisis. Among the countries affected, India- a financial centre with significant worldwide connectivity was impacted directly, leading to an imminent breakdown of economical markets. This paper tries to understand the different aspects of economic challenges faced by the Indian healthcare system and devise the possible measures to overcome the effect of COVID-19 pandemic in India. It analyses the short term and possible long-term impact of this pandemic on the health care sector of India in terms of efficiency and equity. The Indian Government had acted rapidly to allocate funds, resources and manpower. It announced relief packages for the marginalized communities and covered costs. Specific research centres in the country need to work to quickly mitigate any further damage.

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