Comparative efficacy of Annona squamosa and Azadirachta indica extracts against Boophilus microplus Izatnagar isolate

Extracts of eight plants local to Oman, namely Qarat (Acacia nilotica), Mustafal (Annona squamosa), Shereesh (Azadirachta indica), Luban (Boswellia sacra), Kheshkhash (Crotolaria juncea), Zebrot (Jatropha dhofarica) Yas, (Myrtus communis) and Suwwad (Suaeda aegyptiaca) were prepared by steeping shaded dried leaf/ seed powder of each plant in water and solvent (methanol or ethanol). The extracts were tested for their insecticidal and repellent properties against the pulse beetles, Callosobruchus chinensis. The extracts from the seeds of A. squamosa recorded l00% mortality of beetles within twenty and four hours of their exposure to methanol and ethanol extracts, respectively. The other extracts that caused high mortality were from A. nilotica, C. juncea, M. communis and S. aegzptiaca in methanol and B. sacra, J. dhofarica, S. aegptiaca and commercial neem in ethanol. Extracts of M. communis in methanol were highly repellent to the beetles compared to other extracts. Legume seeds treated with extracts of A. squamosa were not repellent, rather the beetles were attracted to them.

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Comparative efficacy of rHaa86 and rBm86 against Hyalomma anatolicum anatolicum and Rhipicephalus (Boophilus) microplus

Parasite Immunology ◽

10.1111/j.1365-3024.2012.01356.x ◽

2012 ◽

Vol 34 (6) ◽

pp. 297-301 ◽

Cited By ~ 14

Author(s):

B. KUMAR ◽

P. AZHAHIANAMBI ◽

D. D. RAY ◽

P. CHAUDHURI ◽

J. De La FUENTE ◽

...

Keyword(s):

Boophilus Microplus ◽

Hyalomma Anatolicum ◽

Comparative Efficacy ◽

Hyalomma Anatolicum Anatolicum

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The Effects of Plant Leaves Variants from The Philippines on Infective Oesophagustonum dentatum Larvae

Veterinary Biomedical and Clinical Journal ◽

10.21776/ub.vetbioclinj.2019.001.01.2 ◽

2019 ◽

Vol 1 (1) ◽

pp. 9-18

Author(s):

Maria Asuncion G. Beltran ◽

◽

Allan Robertson ◽

Richard J. Martin ◽

◽

...

Keyword(s):

Azadirachta Indica ◽

Room Temperature ◽

Moringa Oleifera ◽

Gliricidia Sepium ◽

The Philippines ◽

Ananas Comosus ◽

Aqueous Extracts ◽

Plant Leaves ◽

Annona Squamosa ◽

Chrysophyllum Cainito

Nine aqueous extracts of plant leaves from the Philippines were evaluated against Oesaphagustomum dentatum sensitive (ODSEns) larvae for their anthelmintic effects. The plant species evaluated were Ananas comosus, Chrysophyllum cainito,Gliricidia sepium, Annona squamosa, Moringa oleifera, Tinospora rumphii Boerl,Azadirachta indica, Trichantera gigantea and Leuacaena leucocephala. The leaves of the plants gathered from Camiling, Tarlac, Philippines, were dried under room temperature for 30 days and then ground. Aqueous extracts of these plant leaves were prepared using migration buffer. In vitro migration and inhibition assays were used to evaluate the anthelmintic activity against exsheathed third stage larvae of Oesophagustomum dentatum. The experiments were put in 24-well plates at room temperature with four replications per treatment using different concentrations of 1, 2.5, 5 and 10 mg/ml. About 120 larvae per well were deposited on a larval migration apparatus consisting of 20 μm nylon mesh filters and incubated 20 hours under different treatments and another 2 hours to allow the migration of active motile larvae. The evaluation criteria were based on the average numbers of inhibited larvae under the different treatments. The percentage inhibition were statistically analyzed using Two-Way ANOVA (P=≤.0001) of the Graph Pad Prizm software (V5 San Diego, Ca). The most effective leaves with their highest inhibition level were from Ananas comosus (pineapple, 86.50%), Gliricidia sepium (madre de cacao, 84.50%), Chrysophyllum cainito (star apple, 83.75%), Moringa oleifera (horseradish,58.75%), Annona squamosa (sweet sop, 56.00%), Tinospora rumphii Boerl ( \Makabuhay, 55.75%), and Azadirachta indica (Neem, 51.50%), whereby extremely significant (P˂0.001) effect against O. dentatum larvae. Two leaves extracts, from Trichantera gigantea and Leuacaena leucocephala have no significant effect on O. dentatum larvae. The larval migration assay allows initial studies on the anthelmintic effects of the herbs.

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Comparative study of neem leaf (Azadirachta indica) suspension and toltrazuril against coccidiosis in Sonali chicken

10.33109/bjvmjd19am1 ◽

2020 ◽

Vol 17 (2) ◽

Author(s):

R. Roy ◽

M. H. Hasan ◽

F. B. Aziz ◽

R. Islam ◽

S. Sarkar

Keyword(s):

Body Weight ◽

Azadirachta Indica ◽

Body Weight Gain ◽

Treated Group ◽

The Body ◽

Total Leukocyte Count ◽

Comparative Efficacy ◽

Blood Constituents ◽

Treatment Groups ◽

Neem Leaves

Background: Coccidiosis is recognized as the parasitic disease with the greatest economic impact on poultry industries worldwide. Sonali chicken are commonly affected by coccidiosis in Bangladesh. Recently, the concerns about possible drug resistance have aroused great caution in the usage of drug in the animal industry. As an alternative herbal extracts are used and Neem leaves (Azadirachta indica) have anti-coccidial property. This study was conducted to evaluate the comparative efficacy of Neem leaves suspension and toltrazuril on coccidial load, blood constituents, growth performance of Sonali chicken infected with Eimeria tenella. Methods: The study was conducted in the laboratory and experimental shed under the department of physiology and pharmacology, HSTU, Dinajpur during, 2nd January to 3rd February, 2019. Eighty (80) Sonali chicken of seven days old were randomly divided into four groups named T0, T1 T2 and T3 and each group contained 20 birds. All groups were supplied E. tenella orally except T0 group and after 3 days T2 group was treated with Neem leaves suspension (5%) for 15 days and T3 groups was treated with toltrazuril (topzuril ®) solution (1 ml/lit) for 2 days. Results: Results showed that protozoal load decreased significantly (P<0.01) in T2 & T3 groups whereas increased significantly (P<0.01) in T0 and T1 group. The total erythrocyte count and hemoglobin in different treatment groups were similar and the differences were non-significant (P>0.05) statistically except total leukocyte count (TLC). The body weight of T0, T1, T2 and T3 group at day 30 were 432.65, 469.81, 548.81, 489.22 gm respectively which is statistically significant (P<0.01) and highest body weight gain was recorded from Neem leaves suspension treated group. Conclusion: Neem leaves suspension have significant effects on protozoal load, body weight and no significant effect on haematological parameters except TLC.

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