scholarly journals Phytochemical screening and in vivo antimalarial activity of extracts from three medicinal plants used in malaria treatment in Nigeria

2015 ◽  
Vol 115 (1) ◽  
pp. 299-305 ◽  
Author(s):  
A. E. Bankole ◽  
A. A. Adekunle ◽  
A. A. Sowemimo ◽  
C. E Umebese ◽  
O Abiodun ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antimalarial Activity ◽  
Malaria Treatment ◽  
Phytochemical Screening

scholarly journals Phytochemical Screening and In Vivo Antimalarial Activity of Two Traditionally Used Medicinal Plants of Afar Region, Ethiopia, against Plasmodium berghei in Swiss Albino Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sibhatu Gebrehiwot ◽  
Mohammed Shumbahri ◽  
Amelework Eyado ◽  
Tilahun Yohannes
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Antimalarial Activity ◽  
Lethal Dose ◽  
Negative Control ◽  
Phytochemical Screening ◽  
Salvadora Persica ◽  
Swiss Albino Mice ◽  
Albino Mice

The objective of the present study was to investigate phytochemical components, antiplasmodial activity (in vivo) and evaluate the toxicity of two local medicinal plants, namely, Salvadora persica L. and Balanites rotundifolia (Van Tiegh.) used in Afar ethnomedicine for the treatment of malaria. In this study, phytochemical screening has been done using standard methods and the existence of antiplasmodial compounds was detected in these plant extracts. Four-day Peter’s test was used to determine parasite inhibition, PCV was determined by Wintrob’s method, and effects against loss of body weight and improvements on survival time were determined. LD50s of the crude extracts have been also done. Acute toxicity studies of the extracts were carried out in Swiss albino mice prior to antimalarial activity test. All extracts revealed no obvious acute toxicities on mice up to the highest (5000mg/kg) dose given. The crude extract was estimated to have oral median lethal dose higher than 5,000 mg/kg. With the 4-day suppressive test, both plant extracts demonstrated dose-dependent significant reduction in parasitemia level at all test doses compared to the negative control: in the extract of B. rotundifolia 500 mg/kg extract (60.59±3.25%), 350 mg/kg extract (48.1±1.4), and 200 mg/kg extract (41.33±1.1%) were found. And in case of S. Persica 500 mg/kg extract (50.6±4.01%), 350 mg/kg extract (35.85±0.89), and 200 mg/kg extract (27.69±1.14%) were found. The results of this study provide support for the traditional therapeutic value and the reported antimalarial activity.

In Vivo antimalarial activity of aqueous extracts from Kenyan medicinal plants and their Chloroquine (CQ) potentiation effects against a blood-induced CQ-resistant rodent parasite in mice

2007 ◽  
Vol 21 (4) ◽  
pp. 337-343 ◽  
Author(s):  
Francis W. Muregi ◽  
Akira Ishih ◽  
Tohru Suzuki ◽  
Hideto Kino ◽  
Teruaki Amano ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antimalarial Activity ◽  
Aqueous Extracts

scholarly journals Assessment of Antimalarial Activity againstPlasmodium falciparumand Phytochemical Screening of Some Yemeni Medicinal Plants

2009 ◽  
Vol 6 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Mohammed A. Alshawsh ◽  
Ramzi A. Mothana ◽  
Hassan A. Al-shamahy ◽  
Salah F. Alsllami ◽  
Ulrike Lindequist
Keyword(s):  
Medicinal Plants ◽  
Antimalarial Activity ◽  
Traditional Healers ◽  
Antiplasmodial Activity ◽  
World Health ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Cissus Rotundifolia ◽  
Health Organization

Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa,Azadirachta indica,Cissus rotundifolia,Echium rauwalfii,Dendrosicyos socotranaandBoswellia elongata) commonly used in Yemen by traditional healers for the treatment of malaria as well as other diseases, were collected from different localities of Yemen, dried and extracted with methanol and water successfully. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates ofPlasmodium falciparum. The selectivity parameters to evaluate the efficacy of these medicinal plants were measured byin vitromicro test (Mark III) according to World Health Organization (WHO) 1996 & WHO 2001 protocols of antimalarial drug tests. Among the investigated 12 extracts, three were found to have significant antiplasmodial activity with IC50values less than 4 µg/ml, namely the water extracts ofA. fruticosa,A. indicaandD. socotrana. Six extracts showed moderate activity with IC50values ranging from 10 to 30 µg/ml and three appeared to be inactive with IC50values more than 30 µg/ml. In addition, preliminary phytochemical screening of the methanolic and aqueous extracts indicated the presence of saponins, tannins, flavonoids, terpenoids, polysaccharides and peptides.

scholarly journals In Vitro Antimalarial Activity of Extracts of Some Indigenous Plant Species in Kebbi State

2020 ◽  
pp. 1-10
Author(s):  
Aisha Abdulrazak ◽  
Keyword(s):  
Medicinal Plants ◽  
Aqueous Extract ◽  
Antimalarial Activity ◽  
Antimicrobial Activities ◽  
Antiplasmodial Activity ◽  
Phytochemical Screening ◽  
Indigenous Plants ◽  
Traditional Use ◽  
Water As Solvent

The search for antimalarial compounds has been necessitated by the resistance of Plasmodium falciparum to almost all antimalarial drugs. The aim of this research was to determine in-vitro antimalarial activity of extracts of some indigenous plants species in Kebbi State. Plant extraction was carried-out by maceration using ethanol and water as solvent. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates of P. falciparum using WHO method of in-vitro micro test. Phytochemical screening was also carried out on the extract to deduce the active chemicals present in the plant extract. All plant extracts demonstrate dose dependent antimicrobial activities with IC50 Less than 50%. However highest growth inhibition of the P. falciparum was demonstrated by aqueous and ethanol extract of A. indica with IC50 7.4µg/ml and 8.6µg/ml respectively followed by ethanol and aqueous extract of C. occidentalis with IC50 15.3µg/ml and 18.0µg/ml respectively. Least antimalarial activity was demonstrated by aqueous extract of M. oleifera with IC50 33.5µg/ml while ethanolic extract of M. oleifera demonstrated IC50 of 20.50µg/ml. M. indica ethanolic and aqueous extract also demonstrated moderate antimalarial activity with IC50 18.8µg/ml and 24.5µg/ml. The phytochemical screening of medicinal plants showed the presence of tannins, saponins, alkaloids, flavonoid, phenol and cardiac glycosides in the extracts, which may be responsible for the antiplasmodial activity. This result justifies the traditional use of the plant in malaria treatment and further research is suggested to identify and characterize the active principles from the plants. Keywords: Antimalaria, Invitro, Medicinal Plants, Malaria, Kebbi

scholarly journals Medicinal Plants Used for Malaria Treatment in Gamba Village, North Region of Cameroon: Ethnopharmacological Survey; In Vivo Antimalarial Activity of Aqueous Extracts of Khaya Senegalensis Bark.

2021 ◽  
Author(s):  
Davy-Hyacinthe Anguechia Gouissi ◽  
Roselyne Teponging Nzangue ◽  
Josue Haskandi Kalaza ◽  
Willy Pabo ◽  
Siméon Pierre Fodouop Chegaing
Keyword(s):  
Medicinal Plants ◽  
Traditional Medicine ◽  
Aqueous Extract ◽  
Antimalarial Activity ◽  
Traditional Healers ◽  
Positive Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Khaya Senegalensis

Abstract Background: In traditional medicine, the floral diversity permits the inhabitants of North Cameroon to use a great number of plants to fight against Malaria. The aim of this study was to identify plants used in traditional medicine to treat malaria, and to verify the scientific basis for the use of one of these plants in the locality of Gamba.Methods: An Ethnopharmacological survey was carried out on 15 traditional healers. We collected data on use of medicinal plants using questionnaires. Then in-vivo antimalarial activity of the decoctioned and macerated aqueous extracts of khaya senegalensis trunk bark was evaluated. The 4-day suppressive peters test was realised on mus musculus swiss albino mice. On day one, mice were infected with 107 plasmodium berghei parasitized red blood cells through intra-peritoneal inoculation. 2 hours after infestation, mice in batches of 6 were treated orally at a dose of 75, 150.300 mg/Kg for macerated aqueous extract and 65, 120.260 mg/Kg for decoctioned extract daily during 3 days at an administration volume of 10 ml/Kg. An extract was considered (% reduction): Highly active (between 100-90 %); moderate (between 90-50 %); weak (between 50-10 %); Inactive (between 0 %). P-values <0.05 were considered statistically significant.Results: A total of 18 plant species belonging to 12 families were identified for the preparation of 12 recipes. The decocted aqueous extract of khaya senegalensis showed moderate anti-plasmodial activity (% reduction = 52.46%) at the highest dose of 260 mg/kg with p<0.001 compared to the positive control group. The aqueous macerate at doses of 150 and 300mg/kg gave respectively a percentage reduction of parasitaemia of 59.42% and 71.80% and also showed moderate anti-plasmodial activity; with p<0.001 between the different extracts and the positive control (99.18%).Conclusion: In conclusion, extracts of khaya senegalensis showed moderate anti-plasmodial activity. It would therefore be necessary to evaluate the anti-malarial activity in-vivo and the toxicity of the aqueous extracts macerated using other solvents and also test the other plants listed.

scholarly journals In vivo Anti-plasmodial Activity of Crude Extracts of Three Medicinal Plants Used Traditionally for Malaria Treatment in Kenya

2018 ◽  
Vol 24 (4) ◽  
pp. 1-7
Author(s):  
Ruth Omole ◽  
Hamisi Malebo ◽  
Ramadhani Nondo ◽  
Shaban Katani ◽  
Husna Mbugi ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Malaria Treatment ◽  
Crude Extracts

scholarly journals Phytochemical screening and in vivo antipyretic activity of the aqueous extracts of three Moroccan medicinal plants

2017 ◽  
Vol 4 (4) ◽  
pp. 188 ◽  
Author(s):  
Ghizlane Hajjaj ◽  
Aziz Bahlouli ◽  
Karima Sayah ◽  
Mouna Tajani ◽  
Yahia Cherrah ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Antipyretic Effect ◽  
Antipyretic Activity

Objective: In this study, the antipyretic effect of different extracts obtained from Matricaria chamomilla L. (MC), Ormenis mixta L. (OM) and Pistacia atlantica DESF. (PA) was investigated experimentally in rats.Methods: Antipyretic activity of aqueous extracts was evaluated by yeast induced pyrexia method. The antipyretic effect was retained in all extracts tested and was comparable to that of paracetamol used as the standard drug negative control group (distilled water). The extracts were also phytochemically screened for alkaloids, tannins, saponins, flavonoids, terpenoids, quinones and anthraquinones.Results: Treatment with aqueous extracts at dose of 400 mg/kg showed a significant (p≤0.05) reaction of pyrexia in rodents. The results suggest that this Moroccan medicinal plants possesses potent antipyretic activity. Phytochemical screening of the plants showed the presence of flavonoids, terpenoids, saponins, tannins, alkaloids and quinons which may responsible for this activity. However, anthraquinones were absent in all plants and alkaloids from PA.Conclusions: This study might be the first formal report on antipyretic effects of these three plants in Morocco.

scholarly journals In vivo Antimalarial Activities of Five Ugandan Medicinal Plants on Plasmodium berghei in Mice

2020 ◽  
pp. 1-13
Author(s):  
Clement Olusoji Ajayi ◽  
Anthony Adebolu Elujoba ◽  
Hedmon Okella ◽  
Joseph Oloro ◽  
Atwine Raymond ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Drug Development ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Laboratory Report ◽  
Experimental Laboratory ◽  
Dose Dependent ◽  
Positive Controls

Aim: Medicinal plants have played an important role in the treatment of different ailments including malaria in developing countries particularly in Africa. This study has evaluated the antimalarial activities of Azadirachta indica A. Juss (Meliaceae), Cymbopogon citratus Stapf. (Poaceae), Moringa oleifera Lam. (Moringaceae), Tithonia diversifolia (Hemsl) A. Grey (Asteraceae) and Vernonia amygdalina Del. (Asteraceae) which are commonly-used for malaria treatment in Uganda. Study Design: This is an experimental laboratory report on antimalarial activities of some Ugandan medicinal plants for subsequent profiling in an herbal pharmacopoeia and eventual drug development. Place and Duration of Study: The Animal Research Facility and the Clinical and Research Laboratory, Faculty of Medicine, Mbarara University of Science and Technology, Uganda, between July 2019 and March 2020. Methodology: The antimalarial activity of the hot infusion of each leaf was evaluated on chloroquine-sensitive Plasmodium berghei ANKA-infected mice using 4-day test at 100 – 400 mg/kg with chloroquine (10 mg/kg) and artemether-lumefantrine (4 mg/kg) as positive controls; and distilled water as negative control. The observed haematological responses of the animals were determined with an automated haematometer. Results: The results showed dose-dependent activities in the animals treated with the extract of each plant leaf in varying degrees. Thus, V. amygdalina and T. diversifolia showed the highest antimalarial activities with the chemosuppression values of 75% and 66% at 400 mg/kg, respectively. The results of V. amygdalina, T. diversifolia and M. oleifera, extracts gave the lowest ED50 of 141, 195 and 231 mg/kg, respectively being significantly different from A. indica (ED50 319 mg/kg) and C. citratus (ED50 346 mg/kg) with V. amygdalina as the most potent extract among the five plant leaves. Conclusion: The observed activities of the five plants have therefore supported their folkloric uses as antimalarial remedies by the Ugandan traditional medicine practitioners with obvious potentials for drug development.

scholarly journals In Vitro Antibacterial Activity, Preliminary Phytochemical Screening Profile, and In Vivo Toxicity of Seven Traditional Medicinal Plants in Ethiopia

2022 ◽  
Author(s):  
Eyerus Mekuriaw ◽  
Enat Mengistu ◽  
Ayana Erdedo ◽  
Hassen Mamo
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Phytochemical Content

The threat of antibiotic-resistance calls for novel antibacterial agents. This study was aimed at screening medicinal plants for their antibacterial properties, phytochemical content and safety. Leaves of Allophylus abyssinicus (Hochst.) Radlk., Dicliptera laxata C.B.Clarke, Ligustrum vulgare L., Solanecio gigas (Vatke) c. Jeffrey and Gymnanthemum myrianthum (Hook.f.) H.Rob.; leaf and stem-bark of Olinia rochetiana A. Juss. and the seed of Cucurbita pepo L. were used. Chloroform and ethanol were used to extract G. myrianthum, D. laxata and O. rochetiana; ethyl acetate and methanol for the rest, and water for all. The extracts were tested against clinical/standard strains of Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus by the agar-diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined. Acute toxicity to mice was checked and preliminary phytochemical screening was done. Thirteen extracts, out of 24, were active (inhibition zone >7 mm) at differing levels (9.67±0.33-25.66±0.57 mm) against at least one bacterial strain. The MICs and MBCs were 1.95-15.6 mg/mL and 7.8-125 mg/mL respectively. The aqueous extract of S. gigas, methanol extracts of L. vulgare and A. abyssinicus, and ethanol extract of O. rochetiana leaf were the most active (MIC 1.95mg/ml) against S. aureus. Ethyl acetate extracts of A. abyssinicus, L. vulgare and S. gigas; aqueous of C. pepo, O. rochetiana and G. myrianthum; and all D. laxata had no antibacterial activity. P. aeruginosa was the least susceptible to any extract, although the methanol and aqueous extracts of S. gigas performed better against it. Preliminary phytochemical screening of selected extracts for phenols, flavonoids, tannins, steroids, terpenoids, steroidal glycosides, alkaloids, saponins, resins and glycosides showed positivity at least for four of these phytochemicals with glycoside and terpenoids in nearly all extracts and resin in none. The plants were not toxic to mice at 2000 mg/kg. Further consideration of S. gigas, L. vulgare, A. abyssinicus and O. rochetiana is recommended in light of their promising potential and safety.

Sign in / Sign up

Export Citation Format

Share Document