scholarly journals Phytochemical screening and in vivo antipyretic activity of the aqueous extracts of three Moroccan medicinal plants

2017 ◽  
Vol 4 (4) ◽  
pp. 188 ◽  
Author(s):  
Ghizlane Hajjaj ◽  
Aziz Bahlouli ◽  
Karima Sayah ◽  
Mouna Tajani ◽  
Yahia Cherrah ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Antipyretic Effect ◽  
Antipyretic Activity

Objective: In this study, the antipyretic effect of different extracts obtained from Matricaria chamomilla L. (MC), Ormenis mixta L. (OM) and Pistacia atlantica DESF. (PA) was investigated experimentally in rats.Methods: Antipyretic activity of aqueous extracts was evaluated by yeast induced pyrexia method. The antipyretic effect was retained in all extracts tested and was comparable to that of paracetamol used as the standard drug negative control group (distilled water). The extracts were also phytochemically screened for alkaloids, tannins, saponins, flavonoids, terpenoids, quinones and anthraquinones.Results: Treatment with aqueous extracts at dose of 400 mg/kg showed a significant (p≤0.05) reaction of pyrexia in rodents. The results suggest that this Moroccan medicinal plants possesses potent antipyretic activity. Phytochemical screening of the plants showed the presence of flavonoids, terpenoids, saponins, tannins, alkaloids and quinons which may responsible for this activity. However, anthraquinones were absent in all plants and alkaloids from PA.Conclusions: This study might be the first formal report on antipyretic effects of these three plants in Morocco.

scholarly journals In-vivo Antipyretic Effect of Eel (Anguilla bicolor bicolor) Oil on Yeast-induced Fever on Mice

2019 ◽  
Vol 15 (2) ◽  
pp. 219
Author(s):  
Heru Sasongko ◽  
Aulia Ayu Rahmawati ◽  
Yeni Farida ◽  
Sugiyarto Sugiyarto
Keyword(s):  
Body Weight ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Antipyretic Effect ◽  
Antipyretic Activity ◽  
Epa And Dha

<p>Fish oil has been studied for medicinal purposes, including its antipyretic properties. Eel (<em>Anguilla bicolor bicolor</em>) oil, which contains vitamins and fatty acids, including Omega-3 (EPA and DHA), is also expected to have the antipyretic effect. This research aimed to examine the antipyretic activity of eel oil on white mice (<em>Mus musculus</em> L.). An in-vivo study was done on thirty Swiss-Webster strain males mice that previously got 20% yeast-induced fever. Six treatments were applied including normal group (untreated), a negative control group (yeast-treated), a positive control group treated with acetaminophen (1.764 mg/20 g body weight), and three groups treated with eel oil (0.048, 0.096 and 0.192 g/20 g body weight, respectively). The data was analyzed statistically using one way ANOVA then was continued with LSD post hoc test. The results showed that eel oil has significantly reduced yeast-induced hyperthermia on mice five hours after application at doses 0.096 and 0.192 g/20 g body weight. Our finding suggests that eel oil possess antipyretic properties when was applied in certain doses, and this effect is presumably attributed to its high content of fatty acid, including EPA and DHA.</p>

scholarly journals Anti-Oxidant and Hepatoprotective Effects of Senecio biafrae on CCl4-induced Liver Damage in Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 31-35
Author(s):  
Israel Oghenevwodoko Okoro ◽  
◽  
Helen Ejiro Kadiri ◽  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Distilled Water ◽  
Standard Drug ◽  
Anti Oxidant

Background: The present study was performed to explore whether the aqueous extract of Senecio biafrae (S. biafrae) roots provide any in vivo protective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Methods: Rats (150-200 grams) were grouped into five groups (A-E) of six rats each and were treated orally for twelve days with 72 hourly administration of CCl4 (1 mL/kg) as follows: Group A received distilled water only (negative control), Group B was administered distilled water plus CCl4 (positive control), Group C was administered 400 mg/kg extract and CCl4, Group D received 200 mg/extract and CCl4, while Group E was administered standard drug (Silymarin 25mg/kg, PO). Results: Pre-treatment with the extract of S. biafrae (200 or 400mg/kg) or Silymarin (25mg/kg) caused significant restoration in the biomarkers as evaluated by reducing the levels of malondialdehyde, transaminases and elevating the levels of superoxide dismutase, catalase and glutathione peroxidase activities, which were altered by CCl4 toxicity. The extract at a dose of 400mg/kg demonstrated similar activities comparable to the standard drug (Silymarin). Conclusion: The results of this study indicate that the root extract of S. biafrae possesses hepatoprotective and anti-oxidant properties which may be due to the presence of phytochemicals in it.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

In Vivo Anti-Anemic Effect of an Aqueous Root Extract of Phyllanthus Muellerianus (Kuntze) Exell in Model Rats.

2021 ◽  
Author(s):  
James Nyirenda ◽  
Gershom B. Lwanga ◽  
Kaampwe M. Muzandu ◽  
David K. Chuba ◽  
Gibson M. Sijumbila
Keyword(s):  
Plant Extract ◽  
Hematological Parameters ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Aqueous Root Extract

Abstract Ethnopharmacological relevanceAnemia is a very serious condition in Zambia. One of the plants that has been used traditionally is Phyllanthus muellerianus where different parts of shrub are used to treat a number of diseases in Zambian folklore medicine. Earlier studies have investigated medicinal properties of its aqueous root extracts. This study evaluated the effect of P. muellerianus roots on the hematological indices of albino rats and determined its phytochemical profile. Aim of the studyTo carry out phytochemical screening of the root extract and assess the ant-anemic effect of the aqueous extract on laboratory rats with tail-bled induced anemia Materials and MethodsThirty-six male albino rats placed in six groups were used for the study. The groups comprised the 100, 200, and 400 mg/kg plant extract, Ranferon (200 mg/kg) positive control, anemic non treated control and a normal (non-anemic) control. Anemia, induced through bleeding of the rats, was defined as hemoglobin (Hb) levels less than 12 g/dL. The anti-anemic potential of the plant was determined by comparing its effect on the hematological parameters of rats on treatment to that of the control group.ResultsAfter treatment, rats on the 400 mg/kg plant extract dose showed the greatest increase in the mean values for Hb, Packed cell volume (PCV) and RBC count were 43.3±1.2%, 15.4±0.3 g/dL and 6.3±0.3 x106 /mL respectively, when compared to the negative control group (P < 0.05). Phytochemical screening revealed positive results for alkaloids, flavonoids, saponins, glycosides, steroids, triterpenoids and tannins with varying amounts.Conclusions. The aqueous root extract of P. muellerianus was efficacious against anemia in a dose-dependent manner. The phytochemical compositions seem to be responsible for its hematopoietic properties. Thus, the root decoction of P. muellerianus is useful in alleviating anemia and the results lend credence to its use in traditional medicine in the management of anemia.

scholarly journals Antiproliferation Effects of Nanophytosome-Loaded Phenolic Compounds From Fruit of Juniperus Polycarpos Against Breast Cancer in Mice Model: Synthesis, Characterization and Therapeutic Effects

2021 ◽  
Author(s):  
Soheila Moeini ◽  
Ehsan Karimi ◽  
Ehsan Oskoueian
Keyword(s):  
Breast Cancer ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mice Model ◽  
Fruit Extract ◽  
Phenolic Fraction ◽  
Juniperus Polycarpos

Abstract Background: This research was performed to synthesize nanophytosomes-loaded high phenolic fraction (HPF) from Juniperus polycarpos fruit extract and investigate its antiproliferation effects against breast cancer in mice model. Results: The nanophytosomes-loaded HPF from Juniperus polycarpos fruit extract was synthesized. The mice trial was conducted to determine the possible toxic effects of the synthesized nanophytosomes. The anticancer, pro-apoptotic, and antioxidative activities of the nanophytosomes were determined. The nanophytosomes-loaded HPF had a spherical structure with a size of 176 nm and a polydispersity index coefficient of 0.24. The in-vivo study manifested that nanophytosomes-loaded HPF significantly improved weight gain and food intake compared to the negative control group (p<0.05). The nanophytosomes-loaded HPF significantly enhanced the expression of bax (3.4-fold) and caspase-3 (2.7-fold) genes but reduced bcl2 (3.6-fold) gene expression in tumor cells. The average tumor size was significantly decreased in mice treated with nanophytosomes-loaded HPF (p<0.05). The expression of GPX (2.3-fold) and SOD (2.7-fold) antioxidants in the liver of mice supplemented with nanophytosomes-loaded HPF was significantly developed compared to the negative control (p<0.05). The nanophytosomes-loaded HPF did not show toxicity on normal cells. Conclusion: Our results indicated that nanophytosomes-loaded HPF might be a potential anticancer agent for the breast cancer treatment.

scholarly journals Medicinal Plants Used for Malaria Treatment in Gamba Village, North Region of Cameroon: Ethnopharmacological Survey; In Vivo Antimalarial Activity of Aqueous Extracts of Khaya Senegalensis Bark.

2021 ◽  
Author(s):  
Davy-Hyacinthe Anguechia Gouissi ◽  
Roselyne Teponging Nzangue ◽  
Josue Haskandi Kalaza ◽  
Willy Pabo ◽  
Siméon Pierre Fodouop Chegaing
Keyword(s):  
Medicinal Plants ◽  
Traditional Medicine ◽  
Aqueous Extract ◽  
Antimalarial Activity ◽  
Traditional Healers ◽  
Positive Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Khaya Senegalensis

Abstract Background: In traditional medicine, the floral diversity permits the inhabitants of North Cameroon to use a great number of plants to fight against Malaria. The aim of this study was to identify plants used in traditional medicine to treat malaria, and to verify the scientific basis for the use of one of these plants in the locality of Gamba.Methods: An Ethnopharmacological survey was carried out on 15 traditional healers. We collected data on use of medicinal plants using questionnaires. Then in-vivo antimalarial activity of the decoctioned and macerated aqueous extracts of khaya senegalensis trunk bark was evaluated. The 4-day suppressive peters test was realised on mus musculus swiss albino mice. On day one, mice were infected with 107 plasmodium berghei parasitized red blood cells through intra-peritoneal inoculation. 2 hours after infestation, mice in batches of 6 were treated orally at a dose of 75, 150.300 mg/Kg for macerated aqueous extract and 65, 120.260 mg/Kg for decoctioned extract daily during 3 days at an administration volume of 10 ml/Kg. An extract was considered (% reduction): Highly active (between 100-90 %); moderate (between 90-50 %); weak (between 50-10 %); Inactive (between 0 %). P-values <0.05 were considered statistically significant.Results: A total of 18 plant species belonging to 12 families were identified for the preparation of 12 recipes. The decocted aqueous extract of khaya senegalensis showed moderate anti-plasmodial activity (% reduction = 52.46%) at the highest dose of 260 mg/kg with p<0.001 compared to the positive control group. The aqueous macerate at doses of 150 and 300mg/kg gave respectively a percentage reduction of parasitaemia of 59.42% and 71.80% and also showed moderate anti-plasmodial activity; with p<0.001 between the different extracts and the positive control (99.18%).Conclusion: In conclusion, extracts of khaya senegalensis showed moderate anti-plasmodial activity. It would therefore be necessary to evaluate the anti-malarial activity in-vivo and the toxicity of the aqueous extracts macerated using other solvents and also test the other plants listed.

scholarly journals ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

2018 ◽  
Vol 11 (4) ◽  
pp. 81
Author(s):  
Hanafis Sastra Winata ◽  
Rosidah Rosidah ◽  
Panal Sitorus
Keyword(s):  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Garcinia Xanthochymus ◽  
Anti Inflammatory

 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

scholarly journals THE PROTECTIVE ROLE OF OMEGA-3 AGAINST GENOTOXICITY AND REPRODUCTIVE TOXICITY OF COBALT OXIDE NANOPARTICLES ACUTE TREATMENT IN MALE MICE

2018 ◽  
Vol 11 (5) ◽  
pp. 423
Author(s):  
Nahed A Hussien ◽  
Hanan R. H. Mohamed
Keyword(s):  
Cobalt Oxide ◽  
Negative Control Group ◽  
Protective Role ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes

Objective: Cobalt nanoparticles (NPs), especially cobalt oxide NPs (Co3O4 NPs) are attracting unique shaped NPs that are used in different biomedical applications and medicine. Different in vitro studies report their toxic and carcinogenic effect but limited in vivo studies were present on its genotoxic potential. The present study was aimed to evaluate the genotoxic potential of Co3O4 NPs on bone marrow cells and sperms and the protective role of omega-3 in male albino mice.Methods: Animals were segregated into four groups that were orally treated for 3 consecutive days, Group 1: Negative control; Group 2: Omega-3 (250 mg/kg); Group 3: Co3O4 NPs (20 mg/kg); and Group 4: Combined group (250 mg/kg Omega-3 and Co3O4 NPs 20 mg/kg).Results: The present results show that Co3O4 NPs administration significantly increased number of micronucleated polychromatic erythrocytes (PCEs)/1000 PCEs, sperm abnormalities, and DNA damage, significantly decreased sperm motility and concentration in comparison to negative control group. However, Omega-3 administration in the combined group modulates the genotoxic potential of Co3O4 NPs in comparison to Co3O4 NPs group.Conclusion: The present study reports the genotoxic potential of Co3O4 NPs in vivo and assesses the protective role of Omega-3 administration due to its antioxidant effect.

scholarly journals Study of antiurolithiatic activity of a formulated herbal suspension.

2015 ◽  
Vol 61 (2) ◽  
pp. 41-49 ◽  
Author(s):  
Nishi Saxena ◽  
Ameeta Argal
Keyword(s):  
Urinary Tract ◽  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Negative Control Group ◽  
Negative Control ◽  
Crystal Nucleation ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Standard Drug ◽  
Dose Levels

Summary Urolithiasis is the process of formation of stone in the urinary tract by crystal nucleation, aggregation and retention in the urinary tract. Traditional medicinal plants have been successfully used to overcome urolithiasis. Hence, herbal formulation containing a mixture of plant extracts was prepared and evaluated for the antiurolithiatic activity. This formulation contained alcoholic extracts of fruit of Tribulus terrestris, root of Boerhavia diffusa and leaves of Azadirachta indica Studies were performed in ethylene glycol-induced urolithiasis model using Cystone as a standard drug. Ethylene glycol increases the level of calcium, oxalate and phosphate which are responsible for urolithiasis. The herbal suspension decreased the level of calcium, oxalate and phosphate significantly at doses of 200 and 300 mg/kg when compared to the negative control group. Creatine, uric acid and urea were also decreased significantly at all dose levels. Histopathology has supported these results. The level of LD50 was found to be higher than 2000 mg/kg. Therefore, the prepared formulation has appreciable significant antiurolithiatic activity and is safe for use.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK MENIRAN (Phyllantus niruri L.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN YANG DIINDUKSI VAKSIN DPT-HB

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivana Jansen ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Distilled Water ◽  
Phyllanthus Niruri ◽  
Antipyretic Effect ◽  
Flower Extract ◽  
Hb Vaccine

Abstract: The aim of this study is to investigate the antipyretic effect of leaf-flower extract (Phyllanthus niruri L.) in Wistar rats induced by DPT-HB vaccine. Samples were 15 Wistar rats as test animals and were divided into five groups of three in each. The negative control group was given orally distilled water, positive control group was given paracetamol and 3 experimental groups were given ethanolic leaf-flower extracts (100 mg, 200 mg and 300 mg /200 g body weight of rats). Pyrexia was induced in rats by intramuscular injection of 0.2 ml DPT-HB vaccine. Antipyretic activity was measured at 0, 30, 60, 90, 120, 150, 180 minutes after administration of leaf-flower extract, paracetamol and distilled water. The results showed that leaf-flower extract at a dose of 300 mg /200 g BW decreased the rectal temperature greater than with doses of 100 and 200 mg /200 g BW for 180 minutes of measurement. Conclusion: Leaf-flower extract has antipyretic effect in Wistar rats.Keywords: antipyretic, phyllanthus niruri, DPT-HB, wistar ratAbstrak: Penelitian ini bertujuan untuk menguji efek antipiretik ekstrak meniran (Phyllanthus niruri L.) pada tikus Wistar yang diinduksi vaksin DPT-HB. Metode: Hewan uji yang digunakan adalah 15 ekor tikus Wistar yang dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 3 ekor tikus. Kelompok kontrol negatif diberikan aquades, kelompok kontrol positif diberikan parasetamol, dan 3 kelompok eksperimental diberikan ekstrak tanaman meniran masing-masing dengan dosis 100mg, 200mg dan 300 mg/200 grBB tikus. Induksi demam pada hewan uji menggunakan vaksin DPT-HB 0,2ml secara intramuskular. Pengukuran suhu rektal dilakukan sebelum dan sesudah pemberian vaksin dan setelah pemberian bahan uji yaitu pada menit ke-30, 60, 90, 120, 150 dan 180. Hasil penelitian memperlihatkan pemberian ekstrak meniran dengan dosis 300 mg/200 grBB menunjukkan penurunan suhu rektal lebih besar dibanding dengan dosis 100 dan 200 mg/200 grBB selama 180 menit pengukuran. Simpulan: Ekstrak meniran memiliki efek antipiretik pada tikus Wistar.Kata kunci: antipiretik, phyllanthus niruri, DPT-HB, tikus wistar

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