scholarly journals Assessment of Antimalarial Activity againstPlasmodium falciparumand Phytochemical Screening of Some Yemeni Medicinal Plants

2009 ◽  
Vol 6 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Mohammed A. Alshawsh ◽  
Ramzi A. Mothana ◽  
Hassan A. Al-shamahy ◽  
Salah F. Alsllami ◽  
Ulrike Lindequist
Keyword(s):  
Medicinal Plants ◽  
Antimalarial Activity ◽  
Traditional Healers ◽  
Antiplasmodial Activity ◽  
World Health ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Cissus Rotundifolia ◽  
Health Organization

Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa,Azadirachta indica,Cissus rotundifolia,Echium rauwalfii,Dendrosicyos socotranaandBoswellia elongata) commonly used in Yemen by traditional healers for the treatment of malaria as well as other diseases, were collected from different localities of Yemen, dried and extracted with methanol and water successfully. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates ofPlasmodium falciparum. The selectivity parameters to evaluate the efficacy of these medicinal plants were measured byin vitromicro test (Mark III) according to World Health Organization (WHO) 1996 & WHO 2001 protocols of antimalarial drug tests. Among the investigated 12 extracts, three were found to have significant antiplasmodial activity with IC50values less than 4 µg/ml, namely the water extracts ofA. fruticosa,A. indicaandD. socotrana. Six extracts showed moderate activity with IC50values ranging from 10 to 30 µg/ml and three appeared to be inactive with IC50values more than 30 µg/ml. In addition, preliminary phytochemical screening of the methanolic and aqueous extracts indicated the presence of saponins, tannins, flavonoids, terpenoids, polysaccharides and peptides.

scholarly journals In Vitro Antimalarial Activity of Extracts of Some Indigenous Plant Species in Kebbi State

2020 ◽  
pp. 1-10
Author(s):  
Aisha Abdulrazak ◽  
Keyword(s):  
Medicinal Plants ◽  
Aqueous Extract ◽  
Antimalarial Activity ◽  
Antimicrobial Activities ◽  
Antiplasmodial Activity ◽  
Phytochemical Screening ◽  
Indigenous Plants ◽  
Traditional Use ◽  
Water As Solvent

The search for antimalarial compounds has been necessitated by the resistance of Plasmodium falciparum to almost all antimalarial drugs. The aim of this research was to determine in-vitro antimalarial activity of extracts of some indigenous plants species in Kebbi State. Plant extraction was carried-out by maceration using ethanol and water as solvent. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates of P. falciparum using WHO method of in-vitro micro test. Phytochemical screening was also carried out on the extract to deduce the active chemicals present in the plant extract. All plant extracts demonstrate dose dependent antimicrobial activities with IC50 Less than 50%. However highest growth inhibition of the P. falciparum was demonstrated by aqueous and ethanol extract of A. indica with IC50 7.4µg/ml and 8.6µg/ml respectively followed by ethanol and aqueous extract of C. occidentalis with IC50 15.3µg/ml and 18.0µg/ml respectively. Least antimalarial activity was demonstrated by aqueous extract of M. oleifera with IC50 33.5µg/ml while ethanolic extract of M. oleifera demonstrated IC50 of 20.50µg/ml. M. indica ethanolic and aqueous extract also demonstrated moderate antimalarial activity with IC50 18.8µg/ml and 24.5µg/ml. The phytochemical screening of medicinal plants showed the presence of tannins, saponins, alkaloids, flavonoid, phenol and cardiac glycosides in the extracts, which may be responsible for the antiplasmodial activity. This result justifies the traditional use of the plant in malaria treatment and further research is suggested to identify and characterize the active principles from the plants. Keywords: Antimalaria, Invitro, Medicinal Plants, Malaria, Kebbi

Natural products from the traditional Moroccan pharmacopoeia: A potential lead for the prevention and treatment of COVID-19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 1278-1285
Author(s):  
Mohamed Yafout ◽  
Amine Ousaid ◽  
Ibrahim Sbai El Otmani ◽  
Youssef Khayati ◽  
Amal Ait Haj Said
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Molecular Mechanisms ◽  
Scientific Literature ◽  
Treatment Protocol ◽  
World Health ◽  
The World ◽  
Health Organization ◽  
Promising Source

The new SARS-CoV-2 belonging to the coronaviruses family has caused a pandemic affecting millions of people around the world. This pandemic has been declared by the World Health Organization as an international public health emergency. Although several clinical trials involving a large number of drugs are currently underway, no treatment protocol for COVID-19 has been officially approved so far. Here we demonstrate through a search in the scientific literature that the traditional Moroccan pharmacopoeia, which includes more than 500 medicinal plants, is a fascinating and promising source for the research of natural molecules active against SARS-CoV-2. Multiple in-silico and in-vitro studies showed that some of the medicinal plants used by Moroccans for centuries possess inhibitory activity against SARS-CoV or SARS-CoV-2. These inhibitory activities are achieved through the different molecular mechanisms of virus penetration and replication, or indirectly through stimulation of immunity. Thus, the potential of plants, plant extracts and molecules derived from plants that are traditionally used in Morocco and have activity against SARS-CoV-2, could be explored in the search for a preventive or curative treatment against COVID-19. Furthermore, safe plants or plant extracts that are proven to stimulate immunity could be officially recommended by governments as nutritional supplements.

scholarly journals In VitroAnti-Plasmodial Activity ofTrigonella foenum–graecumL.

2010 ◽  
Vol 7 (4) ◽  
pp. 441-445 ◽  
Author(s):  
M. Palaniswamy ◽  
B. V. Pradeep ◽  
R. Sathya ◽  
J. Angayarkanni
Keyword(s):  
Ethanol Extract ◽  
Traditional Healers ◽  
Active Principle ◽  
Test Plant ◽  
Moderate Activity ◽  
Inhibition Assay ◽  
Phytochemical Screening ◽  
Solvent Systems ◽  
Fenugreek Leaves

Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. For the present study,Trigonella foenum-graecumL. (fenugreek) were collected from Coimbatore, Tamilnadu, India. The test plant has been used in India by traditional healers for the treatment of fever as well as other diseases. The active principle was extracted out in different solvent systems to assess the anti-plasmodial potential, with an aim that they can further be utilized to formulate drugs.In vitroanti-plasmodial assay of the extracted fractions of fenugreek leaves was carried out using laboratory adapted chloroquine sensitive and resistantPlasmodium falciparumisolates. Schizont maturation inhibition assay was adopted to analyze the potential of the extracts. Ethanol extract (50%) seemed to possess profound anti-plasmodial activity with IC50value of 8.75 ± 0.35 µg ml−1and 10.25 ± 0.35 µg ml−1against chloroquine sensitive and resistantP. falciparumisolates, respectively. Among the investigated six fractions of the plant extracts, two were found to have significant anti-plasmodial activity with IC50values <10 µg ml−1, namely ethanol and butanol extracts. Two extracts chloroform and ethyl acetate showed moderate activity with IC50values ranging from 10 to 20 µg ml−1, and the other two extracts, hexane and water appeared to be inactive with IC50values >85 µg ml−1. In addition, preliminary phytochemical screening of the various extracts indicated the presence of alkaloids, saponin, tannin like phenolic compounds, flavonoids and steroids.

scholarly journals Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: a systematic review of literature

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Elahe Tajbakhsh ◽  
Tebit Emmanuel Kwenti ◽  
Parya Kheyri ◽  
Saeed Nezaratizade ◽  
David S. Lindsay ◽  
...  
Keyword(s):  
Systematic Review ◽  
Medicinal Plants ◽  
Plant Species ◽  
Azadirachta Indica ◽  
Antiplasmodial Activity ◽  
Research Articles ◽  
Moderate Activity ◽  
Maytenus Senegalensis

Abstract Background Malaria still constitutes a major public health menace, especially in tropical and subtropical countries. Close to half a million people mainly children in Africa, die every year from the disease. With the rising resistance to frontline drugs (artemisinin-based combinations), there is a need to accelerate the discovery and development of newer anti-malarial drugs. A systematic review was conducted to identify the African medicinal plants with significant antiplasmodial and/or anti-malarial activity, toxicity, as wells as assessing the variation in their activity between study designs (in vitro and in vivo). Methods Key health-related databases including Google Scholar, PubMed, PubMed Central, and Science Direct were searched for relevant literature on the antiplasmodial and anti-malarial activities of African medicinal plants. Results In total, 200 research articles were identified, a majority of which were studies conducted in Nigeria. The selected research articles constituted 722 independent experiments evaluating 502 plant species. Of the 722 studies, 81.9%, 12.4%, and 5.5% were in vitro, in vivo, and combined in vitro and in vivo, respectively. The most frequently investigated plant species were Azadirachta indica, Zanthoxylum chalybeum, Picrilima nitida, and Nauclea latifolia meanwhile Fabaceae, Euphorbiaceae, Annonaceae, Rubiaceae, Rutaceae, Meliaceae, and Lamiaceae were the most frequently investigated plant families. Overall, 248 (34.3%), 241 (33.4%), and 233 (32.3%) of the studies reported very good, good, and moderate activity, respectively. Alchornea cordifolia, Flueggea virosa, Cryptolepis sanguinolenta, Zanthoxylum chalybeum, and Maytenus senegalensis gave consistently very good activity across the different studies. In all, only 31 (4.3%) of studies involved pure compounds and these had significantly (p = 0.044) higher antiplasmodial activity relative to crude extracts. Out of the 198 plant species tested for toxicity, 52 (26.3%) demonstrated some degree of toxicity, with toxicity most frequently reported with Azadirachta indica and Vernonia amygdalina. These species were equally the most frequently inactive plants reported. The leaves were the most frequently reported toxic part of plants used. Furthermore, toxicity was observed to decrease with increasing antiplasmodial activity. Conclusions Although there are many indigenous plants with considerable antiplasmodial and anti-malarial activity, the progress in the development of new anti-malarial drugs from African medicinal plants is still slothful, with only one clinical trial with Cochlospermum planchonii (Bixaceae) conducted to date. There is, therefore, the need to scale up anti-malarial drug discovery in the African region.

scholarly journals Synthesis of Some Novel and Potent Anti-Plasmodial Aminoalkyl Chalcone Derivatives

2020 ◽  
Vol 10 (5) ◽  
pp. 6076-6081
Keyword(s):  
Antimalarial Activity ◽  
World Health ◽  
Mice Model ◽  
Aromatic Rings ◽  
Plasmodium Parasite ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Health Organization ◽  
Death Cases

Malaria remains to be a health and an economic burden to many people living in Sub-Sahara and Africa. According to World health Organization (WHO) in 2017, 219 million cases of malaria worldwide were documented. Its increase by 2 million from the year 2016 resulted in 435 thousand deaths every day among 1190 deaths of young children more than cases 5 per day have been reported. Among the different species of plasmodium parasite, Plasmodium falciparum is mainly responsible for causing malaria in Africa whereas Plasmodium vivax is the most prevalent in countries outside Africa. Africa suffers from malaria a lot. Almost half of the world’s population is at risk of contracting malaria and approximately 90% of the death cases of Malaria in the world appear from Africa. In our research group in past, we have established that chalcones with an aminoalkyl moiety on one of the aromatic rings have promising in vitro antimalarial activity. We successively enhanced the bioavailability from 3% to 25% in mice model with the derivatization of the potential leads with the help of substitutions of the function groups at 4- position from -F to -CF3.

scholarly journals Pharmacognostical, Phytochemical and Antibacterial Studies on the Leaves of Ocimum tenuiflorum Linn. in Cuba

2016 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
Eva Salas Olivet ◽  
José González ◽  
Armando Cuéllar ◽  
Max Monan ◽  
Yuliamny Adames ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Herbal Remedy ◽  
Diagnostic Value ◽  
World Health ◽  
Phytochemical Screening ◽  
Antibacterial Studies ◽  
The World ◽  
Ocimum Tenuiflorum ◽  
Health Organization ◽  
Free Aminoacids

Towards authentication and quality assurance of medicinal plants, pharmacognostic, physicochemical, phytochemical and preliminary antibacterial studies of the leaves of Ocimum tenuiflorum Linn, were carried out. The macroscopic evaluation revealed characters that are of diagnostic value and useful in authentication of the plant. The physicochemical analyses reveals values for moisture content (12.46 %), alcohol extractive (0.5 %), and total ash (11.5 %) which are within the World Health Organization (WHO) standards for crude drug from medicinal plants, except the last one. Phytochemical screening for secondary metabolites revealed the presence of fats, alkaloids, saponins, triterpens/steroids, reductants sugars, flavonoids, free aminoacids or amines, quinones, anthocyanins and mucilage, and phenolic compounds, while resins and balsams, coumarins and tannins were absent. Information obtained from these studies can be used as markers in the identification and standardization of this plant as a herbal remedy and also towards monograph development on the plant.

scholarly journals Antiplasmodial activity of Anthocleista djalonensis leaves extracts against clinical isolates of Plasmodium falciparum and multidrug resistant K1 strains

2020 ◽  
Vol 8 (3) ◽  
pp. 130-138
Author(s):  
Brice Kouakou Bla ◽  
Oléfongo Dagnogo ◽  
Rolland Gueyraud Kipré ◽  
Opportune Gogo Ballé ◽  
Jonhson David Trébissou ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Multidrug Resistant ◽  
Traditional Healers ◽  
Antiplasmodial Activity ◽  
Clinical Isolates ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Primary Health ◽  
Ethanolic Extracts

Information collected from nine (09) traditional healers in the Moronou village in the Department of Toumodi revealed that Anthocleista djanlonensis is regularly used by the population for primary health care in the processing of malaria. Evaluation of the In vitro antiplasmodial activity showed that the aqueous extracts inhibit growth of clinical isolates and chloroquinoresistant strains (K1) with IC50 of 8.29 µg/mL and 10.23 µg/mL while the ethanolic extracts had IC50 of 37.65 µg/mL and 46.07 µg/mL on the same strains respectively. Results of the In vitro antimalarial bioassay showed that aqueous extracts have promising antiplasmodial effects on clinical isolates and on Plasmodium falciparum multidrug resistant K1 strain (3 µg/mL <IC50 <15 µg/mL). Phytochemical screening revealed that the extracts contain mainly alkaloids, polyphenols, polyterpenes and flavonoids

scholarly journals An Overview on Ethnopharmacology of Different Medicinal Plants of Odisha State of India in the Management of Diabetes Mellitus

2021 ◽  
Vol 33 (11) ◽  
pp. 2589-2598
Author(s):  
Deepankar Rath ◽  
Gurudutta Pattnaik ◽  
Biswakanth Kar
Keyword(s):  
Diabetes Mellitus ◽  
Medicinal Plants ◽  
Ricinus Communis ◽  
Cocos Nucifera ◽  
Aloe Vera ◽  
Traditional Healers ◽  
World Health ◽  
Herbal Remedies ◽  
Synthetic Drugs ◽  
Health Organization

Diabetes mellitus, commonly known as diabetes, is a group of metabolic disorder associated with elevated blood glucose level. World health organization recommended the traditional and herbal remedies for the diabetic management. The application of herbal remedies is extremely increased worldwide in the last three decades. Most of the synthetic drugs were discovered from the plant source out of different regions of the world to meet the demand. Several medicinal plants like Gymnema sylvestre, Pterocarpus marsupium, Catharanthus roseus, Trigonella foenum, Annona squamosa, Aegle marmelos, Withania somnifera, Boerhavia diffusa, Boerhavia erecta, Momordica charantia, Cocos nucifera, Ricinus communis, Azadira chtaindica and Aloe vera have been reported to have varying level of hypoglycemic property. One of the factors involved in the evolution of diabetic convolutions is the impairment due to free radicals and hence a compound with antioxidant and antidiabetic potential would be more effective. The present review article was designed to provide an absolute data on these medicinal plant based remedies by using the traditional healers of Odisha state, India.

Effective Antimalarial Activities of α-Hydroxy Diynes Isolated from Ongokea gore

Planta Medica ◽  
2018 ◽  
Vol 84 (11) ◽  
pp. 806-812 ◽  
Author(s):  
Joséphine Ntumba ◽  
Christian Tshiongo ◽  
Michel Mifundu ◽  
Raphäel Robiette ◽  
Kalulu Taba
Keyword(s):  
Seed Oil ◽  
Antimalarial Activity ◽  
World Health ◽  
Diphenyltetrazolium Bromide ◽  
The World ◽  
Health Organization ◽  
Structure Assignment ◽  
Drug Reference

AbstractThree diynes, octadec-17-ene-9,11-diynoate ethyl (1), 8-hydroxy-octadeca-13,17-diene-9,11-diynoate ethyl (2), and 8-hydroxy-octadec-13-ene-9,11-diynoate ethyl (3), were isolated from Ongokea gore seed oil. The structure assignment of these three compounds was based according to chemical and spectroscopic data. They were screened against Plasmodium falciparum, the parasite that causes malaria. In vitro micro-test (Mark III, supported by the World Health Organization) was developed to assess the response of P. falciparum to the isolated three compounds, and statistical analysis were performed for determination of the concentration that inhibits 50% of the parasite maturation (IC50). Two of the three diynes (2 and 3) showed a very effective in vitro antimalarial activity with an IC50 of 4.5 and 1.7 µM, respectively. Compound 3 exhibited better activity than quinine (IC50 1.9 µM), the drug reference, while compound 1 had no antimalarial activity (IC50 > 125 µM). In the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity screening, all compounds showed no toxicity (mean IC50 of 90 µM for each compound).

scholarly journals In Vitro Antiplasmodial Activity and Cytotoxicity of Extracts of Selected Medicinal Plants Used by Traditional Healers of Western Cameroon

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Denis Zofou ◽  
Mathieu Tene ◽  
Moses N. Ngemenya ◽  
Pierre Tane ◽  
Vincent P. K. Titanji
Keyword(s):  
Lactate Dehydrogenase ◽  
Medicinal Plants ◽  
Eucalyptus Globulus ◽  
Stem Bark ◽  
Traditional Healers ◽  
Antiplasmodial Activity ◽  
Vernonia Amygdalina ◽  
Remote Areas ◽  
Dacryodes Edulis

Medicinal plants play a key role in malaria control in Africa, especially in remote areas where health facilities are limited. In order to assess their acclaimed potentials, eleven extracts were prepared from seven selected plants commonly used in Western Cameroon, and tested both for their antiplasmodial activity and cytotoxicity. The antiplasmodial activity was assessed using Lactate Dehydrogenase Assay (pLDH) and the cytotoxicity estimated on LLC-MK2 monkey kidney epithelial cells. Seven extracts from five different plants were significantly active, with very weak or no cytotoxicity. The Dacryodes edulis leaves showed the highest activity (IC50 of 6.45 μg/mL on 3D7 and 8.2 μg/mL on DD2) followed by the leaves of Vernonia amygdalina (IC50 of 8.72 and 11.27 μg/mL on 3D7 and DD2 resp.) and roots of V. amygdalina (IC50 of 8.72 μg/mL on 3D7), Coula edulis leaves (IC50 of 13.80 μg/mL and 5.79 μg/mL on 3D7 and DD2 resp.), Eucalyptus globulus leaves (IC50 of 16.80 μg/mL and 26.45 μg/mL on 3D7 and DD2) and Cuviera longiflora stem bark (IC50 of 20.24 μg/mL and 13.91 μg/mL on 3D7 and DD2). These findings justify the use of five of the seven plants in malaria treatment by traditional healers of Western Cameroon.

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