scholarly journals The use of RP-HPLC–Q-TOF–MS as a powerful tool for wastewater composition profiling and selection of water pollution marker specific to wastewater contamination

2018 ◽  
Vol 149 (9) ◽  
pp. 1595-1604 ◽  
Author(s):  
Dagmara Kempińska ◽  
Agata Kot-Wasik
Keyword(s):  
Water Pollution ◽  
Rp Hplc ◽  
Selection Of ◽  
Tof Ms

scholarly journals Insights into the Potential of Sourdough-Related Lactic Acid Bacteria to Degrade Proteins in Wheat

2020 ◽  
Vol 8 (11) ◽  
pp. 1689 ◽  
Author(s):  
Vera Fraberger ◽  
Martin Ladurner ◽  
Alexandra Nemec ◽  
Clemens Grunwald-Gruber ◽  
Lisa M. Call ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Competitive Elisa ◽  
Starter Cultures ◽  
Bakery Products ◽  
Wheat Sensitivity ◽  
Immunogenic Proteins ◽  
High Degree ◽  
Selection Of ◽  
Tof Ms

Sourdough processing contributes to better digestible wheat-based bakery products, especially due to the proteolytic activity of lactic acid bacteria (LAB). Therefore, sourdough-related LAB were screened for their capacity to degrade immunogenic proteins like gluten and alpha-amylase-trypsin inhibitors (ATIs). Firstly, the growth of 87 isolates was evaluated on a gluten-based medium. Further, the breakdown capacity of selected isolates was determined for gluten with a focus on gliadins by measuring acidification parameters and MALDI-TOF MS protein profiles. ATI degradation after 72 h of incubation within an ATI-based medium was investigated by means of acidification, HPLC, and competitive ELISA. All isolates exhibited the potential to degrade ATIs to a high degree, whereas the gliadin degradation capacity varied more greatly among tested LAB, with Lacticaseibacillus paracasei Lpa4 exhibiting the strongest alterations of the gliadin pattern, followed by Lactiplantibacillus plantarum Lpl5. ATI degradation capacities ranged from 52.3% to 85.0% by HPLC and 22.2% to 70.2% by ELISA, with Lacticaseibacillus paracasei Lpa4 showing superior breakdown properties. Hence, a selection of specific starter cultures can be used in sourdough processing for wheat-based bakery products with reduced gluten and ATI content and, further, better tolerated products for patients suffering from non-celiac wheat sensitivity (NCWS).

scholarly journals Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4347
Author(s):  
You-Ran Jang ◽  
Kyoungwon Cho ◽  
Se Won Kim ◽  
Susan B. Altenbach ◽  
Sun-Hyung Lim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Throughput ◽  
Glutenin Subunits ◽  
Maldi Tof Ms ◽  
Molecular Weights ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Group 2 ◽  
Tof Ms

Because high-molecular-weight glutenin subunits (HMW-GS) are important contributors to wheat end-use quality, there is a need for high-throughput identification of HMW-GS in wheat genetic resources and breeding lines. We developed an optimized method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to distinguish individual HMW-GS by considering the effects of the alkylating reagent in protein extraction, solvent components, dissolving volume, and matrix II components. Using the optimized method, 18 of 22 HMW-GS were successfully identified in standard wheat cultivars by differences in molecular weights or by their associations with other tightly linked subunits. Interestingly, 1Bx7 subunits were divided into 1Bx7 group 1 and 1Bx7 group 2 proteins with molecular weights of about 82,400 and 83,000 Da, respectively. Cultivars containing the 1Bx7 group 2 proteins were distinguished from those containing 1Bx7OE using well-known DNA markers. HMW-GS 1Ax2* and 1Bx6 and 1By8 and 1By8*, which are difficult to distinguish due to very similar molecular weights, were easily identified using RP-HPLC. To validate the method, HMW-GS from 38 Korean wheat varieties previously evaluated by SDS-PAGE combined with RP-HPLC were analyzed by MALDI-TOF-MS. The optimized MALDI-TOF-MS method will be a rapid, high-throughput tool for selecting lines containing desirable HMW-GS for breeding efforts.

Subunit Characteristics of Pig Pancreas Ferritin Revealed by MALDI-TOF MS and RP-HPLC

2008 ◽  
Vol 24 (5) ◽  
pp. 550-556 ◽  
Author(s):  
L HUANG ◽  
Z LIN ◽  
Q LIN ◽  
L LUO ◽  
H HUANG
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Tof Ms

Identification of peptides with antioxidant and antihypertensive capacities by RP-HPLC-Q-TOF-MS in dry fermented camel sausages inoculated with different starter cultures and ripening times

2017 ◽  
Vol 100 ◽  
pp. 708-716 ◽  
Author(s):  
Lobna Mejri ◽  
Romy Vásquez-Villanueva ◽  
Mnasser Hassouna ◽  
María Luisa Marina ◽  
María Concepción García
Keyword(s):  
Starter Cultures ◽  
Rp Hplc ◽  
Tof Ms

Determination and identification of hydrophilic and hydrophobic arsenic species in methanol extract of fresh cod liver by RP-HPLC with simultaneous ICP-MS and ESI-Q-TOF-MS detection

2013 ◽  
Vol 141 (3) ◽  
pp. 3093-3102 ◽  
Author(s):  
Uriel Arroyo-Abad ◽  
Susanne Lischka ◽  
Christian Piechotta ◽  
Jürgen Mattusch ◽  
Thorsten Reemtsma
Keyword(s):  
Methanol Extract ◽  
Arsenic Species ◽  
Ms Detection ◽  
Rp Hplc ◽  
Icp Ms ◽  
Tof Ms

scholarly journals The selection of laccase producing polypores fungi and inducer addition on the degradation of batik waste

2021 ◽  
Vol 948 (1) ◽  
pp. 012052
Author(s):  
A F Mantovany ◽  
I M Artika ◽  
YB Subowo
Keyword(s):  
Water Pollution ◽  
Growth Medium ◽  
Laccase Activity ◽  
Fruiting Bodies ◽  
Science Center ◽  
Forest Park ◽  
Waste Degradation ◽  
Potential Pollution ◽  
Selection Of

Abstract Research on the selection of laccase producing polypores fungi and inducer addition on the degradation of batik waste has been carried out. The objectives of this study were to obtain isolates of polypores fungi with high laccase activity and data on the inducer effect in the batik waste degradation by selected isolate. Batik waste is potential pollution to the environment. It has been found that the Polyporaceae fungus reduce water pollution caused by batik waste. Fungal fruiting bodies were collected at the Cibinong Science Center and forest park IPB Campus Darmaga, Bogor. The inducers used included 15 g/L sucrose, 200 μM CuSO4, and 1.5% sorghum waste. Seven isolates of polypores fungi were obtained. Isolate J6 with high laccase activity was obtained and identified as Coriolopsis sp. The addition of sorghum waste to the growth medium increased the laccase activity of Coriolopsis sp. J6 by 1428 U/mL. Coriolopsis sp. J6 was able to reduce the color of Poly R-478 by 83.73% after the addition of sucrose and incubation for 10 days. This fungus was able to reduce the color of batik waste by 37.17% after the addition of 1.5% sorghum waste and incubation for 10 days.

scholarly journals Comparison of MALDI-TOF-MS and RP-HPLC as Rapid Screening Methods for Wheat Lines With Altered Gliadin Compositions

2020 ◽  
Vol 11 ◽  
Author(s):  
You-Ran Jang ◽  
Kyoungwon Cho ◽  
Sewon Kim ◽  
Jae-Ryeong Sim ◽  
Su-Bin Lee ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Mutation Breeding ◽  
Rapid Screening ◽  
Food Allergies ◽  
Screening Methods ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Wheat Lines ◽  
Tof Ms

The wheat gliadins are a complex group of flour proteins that can trigger celiac disease and serious food allergies. As a result, mutation breeding and biotechnology approaches are being used to develop new wheat lines with reduced immunogenic potential. Key to these efforts is the development of rapid, high-throughput methods that can be used as a first step in selecting lines with altered gliadin contents. In this paper, we optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and reversed-phase high-performance liquid chromatography (RP-HPLC) methods for the separation of gliadins from Triticum aestivum cv. Chinese Spring (CS). We evaluated the quality of the resulting profiles using the complete set of gliadin gene sequences recently obtained from this cultivar as well as a set of aneuploid lines in CS. The gliadins were resolved into 13 peaks by MALDI-TOF-MS. α- or γ-gliadins that contain abundant celiac disease epitopes and are likely targets for efforts to reduce the immunogenicity of flour were found in several peaks. However, other peaks contained multiple α- and γ-gliadins, including one peak with as many as 12 different gliadins. In comparison, separation of proteins by RP-HPLC yielded 28 gliadin peaks, including 13 peaks containing α-gliadins and eight peaks containing γ-gliadins. While the separation of α- and γ-gliadins gliadins achieved by RP-HPLC was better than that achieved by MALDI-TOF-MS, it was not possible to link peaks with individual protein sequences. Both MALDI-TOF-MS and RP-HPLC provided adequate separation of ω-gliadins. While MALDI-TOF-MS is faster and could prove useful in studies that target specific gliadins, RP-HPLC is an effective method that can be applied more broadly to detect changes in gliadin composition.

INDUSTRIAL WASTEWATER TREATMENT BY ADVANCED OXIDATION PROCESSES – A REVIEW

2018 ◽  
Vol 1 (2) ◽  
pp. 24-33
Author(s):  
Dr. Waleed M. Sh. Alabdraba ◽  
Alaa R Al-Obaidi ◽  
Saja S Hashim ◽  
Sara D Zangana.
Keyword(s):  
Water Pollution ◽  
Industrial Wastewater ◽  
Advanced Oxidation Processes ◽  
Chemical Oxidation ◽  
National Standards ◽  
Advanced Treatment ◽  
Treatment Technology ◽  
Oxidation Processes ◽  
Effective Water ◽  
Selection Of

The selection of an effective water treatment technology is the important issues that relatively dealing with water pollution problems, some pollutants need more than the conventional facilities to be treated and discharged within the national standards. This study highlights some of the advanced treatment methods related to chemical oxidation, which is used in the treatment of some types of pollutants such as heavy metals, pesticides, dyes, etc. and demonstrate their effectiveness in treatment by reviewing what has been concluded in a number of studies in this range. © 2018 JASET, International Scholars and Researchers Association  

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