scholarly journals Genome-wide DNA methylome and transcriptome changes induced by inorganic nanoparticles in human kidney cells after chronic exposure

2022 ◽  
Author(s):  
Andrea Soltysova ◽  
Patricia Begerova ◽  
Kristina Jakic ◽  
Katarina Kozics ◽  
Monika Sramkova ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Biomedical Applications ◽  
Human Kidney ◽  
Inorganic Nanoparticles ◽  
Integrated Analysis ◽  
Adverse Health Effects ◽  
Chronic Effects ◽  
Genome Wide ◽  
Detailed Evaluation ◽  
Th 1

AbstractThe unique physicochemical properties make inorganic nanoparticles (INPs) an exciting tool in diagnosis and disease management. However, as INPs are relatively difficult to fully degrade and excrete, their unintended accumulation in the tissue might result in adverse health effects. Herein, we provide a methylome–transcriptome framework for chronic effects of INPs, commonly used in biomedical applications, in human kidney TH-1 cells. Renal clearance is one of the most important routes of nanoparticle excretion; therefore, a detailed evaluation of nanoparticle-mediated nephrotoxicity is an important task. Integrated analysis of methylome and transcriptome changes induced by INPs (PEG-AuNPs, Fe3O4NPs, SiO2NPs, and TiO2NPs) revealed significantly deregulated genes with functional classification in immune response, DNA damage, and cancer-related pathways. Although most deregulated genes were unique to individual INPs, a relatively high proportion of them encoded the transcription factors. Interestingly, FOS hypermethylation inversely correlating with gene expression was associated with all INPs exposures. Our study emphasizes the need for a more comprehensive investigation of INPs’ biological safety, especially after chronic exposure. Graphical abstract

scholarly journals A Genome-Wide Association Study for Hypertensive Kidney Disease in Korean Men

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 751
Author(s):  
Hye-Rim Kim ◽  
Hyun-Seok Jin ◽  
Yong-Bin Eom
Keyword(s):  
Blood Pressure ◽  
Kidney Disease ◽  
Association Study ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Integrated Analysis ◽  
Eqtl Analysis ◽  
Strong Linkage Disequilibrium ◽  
Genome Wide ◽  
A Genome

Hypertension is one of the major risk factors for chronic kidney disease (CKD), and the coexistence of hypertension and CKD increases morbidity and mortality. Although many genetic factors have been identified separately for hypertension and kidney disease, studies specifically focused on hypertensive kidney disease (HKD) have been rare. Therefore, this study aimed to identify loci or genes associated with HKD. A genome-wide association study (GWAS) was conducted using two Korean cohorts, the Health Examinee (HEXA) and Korean Association REsource (KARE). Consequently, 19 single nucleotide polymorphisms (SNPs) were found to be significantly associated with HKD in the discovery and replication phases (p < 5 × 10−8, p < 0.05, respectively). We further analyzed HKD-related traits such as the estimated glomerular filtration rate (eGFR), creatinine, blood urea nitrogen (BUN), systolic blood pressure (SBP) and diastolic blood pressure (DBP) at the 14q21.2 locus, which showed a strong linkage disequilibrium (LD). Expression quantitative trait loci (eQTL) analysis was also performed to determine whether HKD-related SNPs affect gene expression changes in glomerular and arterial tissues. The results suggested that the FANCM gene may affect the development of HKD through an integrated analysis of eQTL and GWAS and was the most significantly associated candidate gene. Taken together, this study indicated that the FANCM gene is involved in the pathogenesis of HKD. Additionally, our results will be useful in prioritizing other genes for further experiments.

scholarly journals (Intra)Cellular Stability of Inorganic Nanoparticles: Effects on Cytotoxicity, Particle Functionality, and Biomedical Applications

2015 ◽  
Vol 115 (5) ◽  
pp. 2109-2135 ◽  
Author(s):  
Stefaan J. Soenen ◽  
Wolfgang J. Parak ◽  
Joanna Rejman ◽  
Bella Manshian
Keyword(s):  
Biomedical Applications ◽  
Inorganic Nanoparticles

scholarly journals Identification of gene expression and DNA methylation of SERPINA5 and TIMP1 as novel prognostic markers in lower-grade gliomas

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9262
Author(s):  
Wen-Jing Zeng ◽  
Yong-Long Yang ◽  
Zhi-Peng Wen ◽  
Peng Chen ◽  
Xiao-Ping Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Classification System ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Lower Grade ◽  
Kaplan Meier ◽  
Genome Wide ◽  
Genes Expression ◽  
Tumors Classification ◽  
Long Term Survivors

Background Lower-grade gliomas (LGGs) is characteristic with great difference in prognosis. Due to limited prognostic biomarkers, it is urgent to identify more molecular markers to provide a more objective and accurate tumor classification system for LGGs. Methods In the current study, we performed an integrated analysis of gene expression data and genome-wide methylation data to determine novel prognostic genes and methylation sites in LGGs. Results To determine genes that differentially expressed between 44 short-term survivors (<2 years) and 48 long-term survivors (≥2 years), we searched LGGs TCGA RNA-seq dataset and identified 106 differentially expressed genes. SERPINA5 and TIMP1 were selected for further study. Kaplan–Meier plots showed that SERPINA5 and TIMP1 expression were significantly correlated with overall survival (OS) and relapse-free survival (RFS) in TCGA LGGs patients. We next validated the correlation between the candidate genes expression and clinical outcome in CGGA LGGs patients. Multivariate analysis showed that TIMP1 mRNA expression had a significant prognostic value independent of other variables (HR = 4.825, 95% CI = 1.370–17.000, P = 0.014). Then, differential methylation sites were identified from differentially candidate gene expression groups, and all four methylation sites were significantly negatively correlated with gene expression (spearman r <  − 0.5, P < 0.0001). Moreover, hyper-methylation of four methylation sites indicated better OS (P < 0.05), and three of them also shown statistical significantly association with better RFS, except for SERPINA5 cg15509705 (P = 0.0762). Conclusion Taken together, these findings indicated that the gene expression and methylation of SERPINA5 and TIMP1 may serve as prognostic predictors in LGGs and may help to precise the current histology-based tumors classification system and to provide better stratification for future clinical trials.

scholarly journals The chronic effects of salinity on the Crustacean Moina affinis Birge (1893)

2004 ◽  
Vol 1 (2) ◽  
pp. 200-206
Author(s):  
Baghdad Science Journal
Keyword(s):  
Life Cycle ◽  
Fresh Water ◽  
Chronic Exposure ◽  
Exposure Period ◽  
Control Group ◽  
Length Growth ◽  
Chronic Effects ◽  
Final Length

Thi s study included the effect of chronic exposure of di fferent concentrations of Nan on the biology of fresh water zroDplonk ton species Moina 41.112' ( Cladcxe'ra The concentrations used for chronic exposure were 03, 0.75. 1 960 to investigate iI effects on the life cycle and la lables of this species after exposure period and the range of this exposure effects on the rate of expectation for further life, also the effect of salinity on reproduotive parameters was studied which included average of total number of eggs and young produced, volume clutch's (6.95. indiclutch for control group , 14.516 indi clutch for 0.596a ,3_65 ind./ clutch for (175 5(.. and 03 Ind} cruel] for 1960) and number of Clutch's (5.5 clutch/ female for control group, 1.7 clutch/ funEoc for 0.5.9(z.z, 1:2 dutch?' female for 0,75960 and 0,1 oluchi female for 196.) which howed a reduction_ But had no effects on the time 10 tarsi clutch produced. Time of surviving ( 12.9 day for control g.roup. 63 day for 0.5%0, 15_2 day for 0.75%.0 and 4.6 day for I 9f,), average final length and average of daily length, growth increasing were studied_ In addition to the studying the orient of salinity on the number of moults which showed a reduction. in its number.

GENOMIZER: an integrated analysis system for genome-wide association data

2006 ◽  
Vol 27 (6) ◽  
pp. 583-588 ◽  
Author(s):  
Andre Franke ◽  
Andreas Wollstein ◽  
Markus Teuber ◽  
Michael Wittig ◽  
Tim Lu ◽  
...  
Keyword(s):  
Genome Wide Association ◽  
Integrated Analysis ◽  
Genome Wide ◽  
Association Data ◽  
Analysis System

Functionalised Inorganic Nanoparticles for Biomedical Applications

Nanoscience ◽  
2009 ◽  
pp. 129-170 ◽  
Author(s):  
E. Duguet ◽  
M. Treguer-Delapierre ◽  
M.-H. Delville
Keyword(s):  
Biomedical Applications ◽  
Inorganic Nanoparticles

scholarly journals Review of Therapeutic Applications of Radiolabeled Functional Nanomaterials

2019 ◽  
Vol 20 (9) ◽  
pp. 2323 ◽  
Author(s):  
Jongho Jeon
Keyword(s):  
Cancer Therapy ◽  
Anticancer Agents ◽  
Biomedical Applications ◽  
Recent Progress ◽  
Medical Science ◽  
Inorganic Nanoparticles ◽  
Original Material ◽  
Functional Nanomaterials ◽  
Therapeutic Applications ◽  
Physical And Chemical

In the last two decades, various nanomaterials have attracted increasing attention in medical science owing to their unique physical and chemical characteristics. Incorporating radionuclides into conventionally used nanomaterials can confer useful additional properties compared to the original material. Therefore, various radionuclides have been used to synthesize functional nanomaterials for biomedical applications. In particular, several α- or β-emitter-labeled organic and inorganic nanoparticles have been extensively investigated for efficient and targeted cancer treatment. This article reviews recent progress in cancer therapy using radiolabeled nanomaterials including inorganic, polymeric, and carbon-based materials and liposomes. We first provide an overview of radiolabeling methods for preparing anticancer agents that have been investigated recently in preclinical studies. Next, we discuss the therapeutic applications and effectiveness of α- or β-emitter-incorporated nanomaterials in animal models and the emerging possibilities of these nanomaterials in cancer therapy.

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