Analysis of genetic diversity in a sweet potato (Ipomoea batatas L.) germplasm collection from Tanzania as revealed by AFLP

Sweet potato (Ipomoea batatas L. Lam) is an important food crop widely cultivated in the world. In this study, nine chloroplast simple sequence repeat (cpSSR) markers were used to analyze the genetic diversity and relationships of 558 sweet potato accessions in the germplasm collection of the National Agrobiodiversity Center (NAC). Eight of the nine cpSSR showed polymorphisms, while Ibcp31 did not. The number of alleles per locus ranged from two to four. In general, the Shannon index for each cpSSR ranged from 0.280 to 1.123 and the diversity indices and unbiased diversity ranged from 0.148 to 0.626, and 0.210 to 0.627, respectively. Results of the median-joining network showed 33 chlorotypes in 558 sweet potato accessions. In factor analysis, 558 sweet potato accessions were divided into four clusters, with clusters I and II composed only of the sweet potato accessions from Korea, Japan, Taiwan, and the USA. The results of this study confirmed that the genetic diversity of the female parents of sweet potato accessions conserved at the NAC is low and therefore more sweet potato accessions need to be collected. These results will help to establish an efficient management plan for sweet potato genetic germplasms at the NAC.

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GENETIC DIVERSITY AMONG SWEET POTATO CROPS CULTIVATED BY TRADITIONAL FARMERS

Revista Caatinga ◽

10.1590/1983-21252018v31n329rc ◽

2018 ◽

Vol 31 (3) ◽

pp. 779-790 ◽

Cited By ~ 1

Author(s):

PABLO FORLAN VARGAS ◽

ERIC WATZKE ENGELKING ◽

LUIS CARLOS FERREIRA DE ALMEIDA ◽

ELIEL ALVES FERREIRA ◽

HAMILTON CESAR DE OLIVERIA CHARLO

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Block Design ◽

Genetic Material ◽

Morphological Characteristics ◽

Leaf Size ◽

Stem Length ◽

Phenotypic Correlation ◽

Crop Species

ABSTRACT The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits.

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Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

Genetics and Molecular Biology ◽

10.1590/s1415-47572008000400020 ◽

2008 ◽

Vol 31 (3) ◽

pp. 725-733 ◽

Cited By ~ 35

Author(s):

Elizabeth Ann Veasey ◽

Aline Borges ◽

Mariana Silva Rosa ◽

Jurema R. Queiroz-Silva ◽

Eduardo de Andrade Bressan ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Sweet Potato ◽

Ipomoea Batatas

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Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha49112222 ◽

2021 ◽

Vol 49 (1) ◽

pp. 12222

Author(s):

Carlos G. ANDRADE ◽

Emanuel M. Da SILVA ◽

Carla RAGONEZI ◽

Miguel Â. A. PINHEIRO DE CARVALHO

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Viral Infections ◽

Catalytic Domain ◽

Sequence Similarity ◽

Vegetative Reproduction ◽

Germplasm Collection ◽

Probability Method ◽

Infection Prevalence ◽

Sequence Identity

Ipomoea batatas (L.) Lam. commonly known as sweet potato, is an important staple food worldwide, mainly due to its high nutritional value and yield. However, vegetative reproduction of sweet potato makes it more susceptible to viral infections, which threatens its productivity, quality, and difficult long-term preservation in germplasm banks. Also, it can act as a virus reservoir infecting the rest of the plant accessions in the bank collections. Hence, this work aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars from the germplasm collection of the ISOPlexis Germplasm Bank, Madeira, Portugal. The infection prevalence by these viruses among cultivars was 81.25%, 25.00%, and 6.25%, respectively; being ISOP1011 the only accession coinfected by Potyvirus and Carlavirus. The accessions ISOP1006, ISOP1010, and ISOP1047 were also coinfected by Begomovirus and Potyvirus, highlighting their vulnerability to viral infections. The ISOP1005 and ISOP1027 accessions were the only ones not infected by any of these viruses. The analysis of the partial sequence obtained from the Carlavirus detected in the accession ISOP1011, revealed the existence of an ORF that encodes for 93 amino acids of the catalytic domain of an RNA-directed RNA polymerase related to the Tymovirus protein family, as could be confirmed by comparison with proteins stored in UniProtKB. Multiple sequence alignment with these proteins showed that Motifs A and B of the catalytic domain were conserved. The search for sequence similarity with sequences deposited in GenBank reported a high sequence identity with Sweet potato yellow mottle virus (SPYMV) and Sweet potato chlorotic fleck virus (SPCFV). However, the 9-11% discrepancy in nucleotide sequence identity and a phylogenetic analysis carried out using the maximum probability method suggests the virus isolated from ISOP1011 is a new divergent strain of the SPCFV species.

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Genetic diversity analysis of sweet potato [Ipomoea batatas (L.) Lam.] germplasms through RAPD and ISSR markers

Bangladesh Journal of Botany ◽

10.3329/bjb.v50i1.52679 ◽

2021 ◽

Vol 50 (1) ◽

pp. 119-129

Author(s):

PN Narasimha Murthy ◽

NB Patel ◽

Chintan Kapadia ◽

KD Desai ◽

G Koteswara Rao

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Genetic Relatedness ◽

Issr Markers ◽

Diversity Analysis ◽

Genetic Diversity Analysis ◽

Polymorphic Loci ◽

Improvement Programme ◽

Rapd And Issr

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.

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On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

Heredity ◽

10.1038/hdy.2013.14 ◽

2013 ◽

Vol 110 (6) ◽

pp. 594-604 ◽

Cited By ~ 32

Author(s):

C Roullier ◽

R Kambouo ◽

J Paofa ◽

D McKey ◽

V Lebot

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

New Guinea ◽

Centre Of Diversity

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Studies on Genetic Diversity for Yield, Growth and Quality Traits in Sweet Potato [Ipomoea batatas (L.) Lam.]

International Journal of Current Research in Biosciences and Plant Biology ◽

10.20546/ijcrbp.2017.404.017 ◽

2017 ◽

Vol 4 (4) ◽

pp. 113-117

Author(s):

Devesh Pratap Singh ◽

Chandra Deo ◽

Deepak Kumar Gautam ◽

Rajesh Kumar ◽

Pushpendra Kumar

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Quality Traits

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Genetic Diversity of Local and Introduced Sweet Potato [Ipomoea batatas (L.) Lam.] Collections for Agro-morphology and Physicochemical Attributes in Ethiopia

Science Technology and Arts Research Journal ◽

10.4314/star.v4i1.2 ◽

2015 ◽

Vol 4 (1) ◽

pp. 9 ◽

Cited By ~ 3

Author(s):

W Mohammed ◽

S Ali ◽

B Shimelis ◽

S Burga

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas

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DIVERSITAS GENETIK KERABAT LIAR UBI JALAR IPOMOEA TRIFIDA (H.B.K.) G.DON. BERUMBI ASAL CITATAH JAWA BARAT BERDASARKAN KARAKTER KROMOSOM (Genetic Diversity Of Wild Relative Of Sweet Potato Tubered-Ipomoea Trifida (H.B.K.) G.don. Originated From Citatah West Java Based On Chromosome Characters)

Indonesian Journal of Applied Sciences ◽

10.24198/ijas.v3i3.15053 ◽

2013 ◽

Vol 3 (3) ◽

Author(s):

Tia Setiawati

Keyword(s):

Genetic Diversity ◽

Principal Component Analysis ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Principal Component ◽

Component Analysis ◽

West Java ◽

Ipomoea Trifida ◽

Plant Materials ◽

Materials Used

AbstrakKerabat liar ubi jalar Ipomoea trifida asal Citatah Jawa Barat potensial digunakan dalam program pemuliaan tanaman ubi jalar (Ipomoea batatas (L.) Lam.) Untuk mengetahui diversitas genetik I. trifida dilakukan pengamatan terhadap karakter kromosom. Bahan tanaman yang digunakan adalah 10 spesies I. trifida berumbi. Hubungan kekerabatan antar spesies diketahui melalui analisis klaster dan Analisis Komponen Utama/Principal Component Analysis (PCA). Hasil pengamatan menunjukkan pengujian pada 10 aksesi I. trifida berumbi dengan menggunakan 9 karakter kromosom menghasilkan jarak ketidakmiripan (Euclidean coeffisien) yang berkisar 1,75 – 6,22 dan menunjukkan diversitas yang luas. Dendogram yang dihasilkan pada jarak ketidakmiripan 5,23 menunjukkan terbentuknya 3 klaster utama. Analisis Komponen Utama (PCA) menghasilkan 2 komponen utama pertama (PC1 dan PC2) yang telah dapat menjelaskan 89,64% dari total variasi. Kata kunci : Ipomoea trifida, Analisis Klaster, Analisis Komponen UtamaAbstractWild relatives of sweet potato, Ipomoea trifida originated from Citatah West Java was potential use in plant breeding programs of Ipomoea batatas (L.) Lam. To determine the genetic diversity of I. trifida, observation on characters of chromosomes of I.trifida was conducted. Plant materials used ten accessions of tubered-I. trifida. Relationship between species identified by cluster analysis and Principal Component Analysis (PCA). The results showed that the observation on the 10 accession of tubered-I. trifida using 9 characters chromosome produces dissimilarities distance (Euclidean coefficient) ranging from 1.75 to 6.22. Dendogram generated at a dissimilarity distance of 5.23 showed the formation of three main clusters. Principal Component Analysis (PCA) produced first two principal component (PC1 and PC2), which has been able to explain 89.64% of the total variation.Keyword : Ipomoea trifida, Clustering Analysis, Principal Component Analysis

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Analysis of genetic diversity in a sweetpotato (Ipomoea batatas) germplasm collection using DNA amplification fingerprinting

Genome ◽

10.1139/g95-123 ◽

1995 ◽

Vol 38 (5) ◽

pp. 938-945 ◽

Cited By ~ 39

Author(s):

Guohao He ◽

C. S. Prakash ◽

R. L. Jarret

Keyword(s):

Genetic Diversity ◽

Ipomoea Batatas ◽

Genetic Relationships ◽

Germplasm Collection ◽

Dna Amplification ◽

Taq Polymerase ◽

Plant Introductions ◽

Vinyl Polymer ◽

High Level ◽

Selection Of

A DNA amplification fingerprinting (DAF) approach was employed to develop individual-specific profiles and analyze genetic relationships among 73 plant introductions of sweetpotato (Ipomoea batatas (L.) Lam.) including unadapted lines from around the world and a few selected U.S.A. cultivars. Reliable and informative fingerprint profiles were obtained employing single octamer primers and Stoffel fragment Taq polymerase in the polymerase chain reaction, polyacrylamide-based vinyl polymer for electrophoresis, and silver staining to visualize the DNA. Using seven highly informative octamer primers, individual-specific DAF profiles were obtained for all accessions tested. The degree of polymorphism in the sweetpotato collection was very large, indicating a high level of genetic variability. Several accessions clustered together based on their geographic source. Most U.S.A. cultivars formed a separate cluster in the phenogram, while accessions from Papua New Guinea exhibited the highest genetic diversity. The wild species I. triloba and tetraploid I. batatas formed a group distinct from the cultivated sweetpotato. DAF appears to be useful in sweetpotato germplasm characterization and may be employed to identify duplicate accessions or for creation of core subsets. DAF data may also be useful for facilitating the selection of parents for a breeding program to ensure a broad genetic base.Key words: DNA fingerprinting, DNA markers, germplasm, genetic diversity, RAPD markers.

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