Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves

2011 ◽  
Vol 65 (2) ◽  
pp. 315-325 ◽  
Author(s):  
Michael Moustakas ◽  
Ilektra Sperdouli ◽  
Theodora Kouna ◽  
Chrysovalantou-Irene Antonopoulou ◽  
Ioannis Therios
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Photosystem Ii ◽  
Soluble Sugar ◽  
Sugar Accumulation ◽  
Stress Effects ◽  
Exogenous Proline

scholarly journals Elevated CO2 Alleviates the Effects of Drought on Wheat - Aphid Interaction

2021 ◽  
Author(s):  
Haicui Xie ◽  
Fengyu Shi ◽  
Jingshi Li ◽  
miaomai Yu ◽  
Jia Fan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nutritional Quality ◽  
Soluble Sugar ◽  
Amino Acid Content ◽  
Sugar Accumulation ◽  
Negative Effects ◽  
Effects Of Drought ◽  
Insect Interactions ◽  
Positive Effect ◽  
Aphid Populations

Abstract Due to rising concentration of atmospheric CO2, climate change is predicted to intensify episodes of drought, however, our understanding of how combined environmental conditions will influence crop-insect interactions is limited. The direct effects of elevated CO2 and drought stress on wheat (Triticum aestivum L.) nutritional quality, insect resistance and their indirect effects on the grain aphid (Sitobion avenae) performance are reported here. Elevated CO2 was able to alleviate low water content in wheat caused by drought stress. Both elevated CO2 and drought promoted soluble sugar accumulation in wheat. However, elevated CO2 decreased and drought increased the amino acid content in wheat. Elevated CO2 induced the down-regulation of jasmonic acid (JA) -dependent defense, but up-regulated the salicylic acid-dependent defense. Drought enhanced abscisic acid accumulation that promoted the JA-dependent defense in wheat. Aphid-induced phytohormone resistance in wheat was not influenced by elevated CO2 and drought. The negative effects of drought on the performance of the aphid population was offset by positive effect of elevated CO2. In conclusion, elevated CO2 can alleviate the effects of drought stress on wheat nutritional quality and resistance, which results in unchanged damage to wheat from aphid populations under future elevated CO2 and drier conditions.

scholarly journals Leaf Age-Dependent Photosystem II Photochemistry and Oxidative Stress Responses to Drought Stress in Arabidopsis thaliana Are Modulated by Flavonoid Accumulation

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4157
Author(s):  
Ilektra Sperdouli ◽  
Julietta Moustaka ◽  
Georgia Ouzounidou ◽  
Michael Moustakas
Keyword(s):  
Lipid Peroxidation ◽  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Photosystem Ii ◽  
Stress Responses ◽  
Heat Dissipation ◽  
Leaf Age ◽  
Psii Photochemistry ◽  
Young Leaves ◽  
Flavonoid Accumulation

We investigated flavonoid accumulation and lipid peroxidation in young leaves (YL) and mature leaves (ML) of Arabidopsis thaliana plants, whose watering stopped 24 h before sampling, characterized as onset of drought stress (OnDS), six days before sampling, characterized as mild drought stress (MiDS), and ten days before sampling, characterized as moderate drought stress (MoDS). The response to drought stress (DS) of photosystem II (PSII) photochemistry, in both leaf types, was evaluated by estimating the allocation of absorbed light to photochemistry (ΦPSII), to heat dissipation by regulated non-photochemical energy loss (ΦNPQ) and to non-regulated energy dissipated in PSII (ΦNO). Young leaves were better protected at MoDS than ML leaves, by having higher concentration of flavonoids that promote acclimation of YL PSII photochemistry to MoDS, showing lower lipid peroxidation and excitation pressure (1 − qp). Young leaves at MoDS possessed lower 1 − qp values and lower excess excitation energy (EXC), not only compared to MoDS ML, but even to MiDS YL. They also possessed a higher capacity to maintain low ΦNO, suggesting a lower singlet oxygen (1O2) generation. Our results highlight that leaves of different developmental stage may display different responses to DS, due to differential accumulation of metabolites, and imply that PSII photochemistry in Arabidopsis thaliana may not show a dose dependent DS response.

scholarly journals Enhanced tolerance to drought stress resulting from Caragana korshinskii CkWRKY33 in transgenic Arabidopsis thaliana

2020 ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Water Soluble ◽  
Caragana Korshinskii ◽  
Group I ◽  
Relative Water

Abstract Background: It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.Results: In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative water, soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment.Conclusions: This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

scholarly journals Enhanced Tolerance to Drought Stress Resulting from Caragana Korshinskii CkWRKY33 in Transgenic Arabidopsis Thaliana

2020 ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Water Soluble ◽  
Caragana Korshinskii ◽  
Group I ◽  
Relative Water

Abstract Background: It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.Results: In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative water, soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment. Conclusions: This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

scholarly journals Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bruno Paes de Melo ◽  
Isabela Tristan Lourenço-Tessutti ◽  
Joaquin Felipe Roca Paixão ◽  
Daniel David Noriega ◽  
Maria Cristina Mattar Silva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Water Deficit ◽  
Soluble Sugar ◽  
Physiological Adaptation ◽  
Sugar Accumulation ◽  
Cellular Mechanisms ◽  
Physiological Performance ◽  
Severe Water Deficit ◽  
Element Binding Protein

Abstract Plants are sessile organisms, which are vulnerable to environmental stresses. As such, plants have developed multiple molecular, physiological, and cellular mechanisms to cope with natural stressors. However, these environmental adversities, including drought, are sources of the main agribusiness problems since they interfere with plant growth and productivity. Particularly under water deprivation conditions, the abscisic acid-responsive element-binding protein AREB1/ABF2 plays an important role in drought stress response and physiological adaptation. In this investigation, we provide substantial confirmation for the role of AREB1/ABF2 in plant survival under severe water deficit using the CRISPR activation (CRISPRa) technique to enhance the AREB1 gene expression. In our strategy, the inactive nuclease dCas9 was fused with an Arabidopsis histone acetyltransferase 1, which improves gene expression by remodeling chromatin. The AREB1 overexpression promotes an improvement in the physiological performance of the transgenic homozygous plants under drought, which was associated with an increase in chlorophyll content, antioxidant enzyme activity, and soluble sugar accumulation, leading to lower reactive oxygen species accumulation. Finally, we found that the CRISPR-mediated up-regulation of AREB1 changes the abundance of several downstream ABA-inducible genes, allowing us to report that CRISPRa dCas9-HAT is a valuable biotechnological tool to improve drought stress tolerance through the positive regulation of AREB1.

scholarly journals Enhanced tolerance to drought stress resulting from Caragana korshinskii CkWRKY33 in transgenic Arabidopsis thaliana

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Wild Type ◽  
Sequence Structure ◽  
Caragana Korshinskii ◽  
Group I

Abstract Background It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses. Results In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants, CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment. Conclusions This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants

Morphological, Physiological and Biochemical Responses of Poplar Plants to Drought Stress

2018 ◽  
Vol 5 (03) ◽  
Author(s):  
ARADHNA KUMARI ◽  
IM KHAN ◽  
ANIL KUMAR SINGH ◽  
SANTOSH KUMAR SINGH
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Biochemical Parameters ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Field Capacity ◽  
Drought Event ◽  
Total Biomass ◽  
Biochemical Responses ◽  
Physiological And Biochemical

Poplar clone Kranti was selected to assess the morphological, physiological and biochemical responses under drought at different levels of water stress, as it is a common clone used to be grown in Uttarakhand for making paper and plywood. The cuttings of Populus deltoides L. (clone Kranti) were exposed to four different watering regimes (100, 75, 50 and 25% of the field capacity) and changes in physiological and biochemical parameters related with drought tolerance were recorded. Alterations in physiological (i.e. decrease in relative water content) and biochemical parameters (i.e. increase in proline and soluble sugar content and build-up of malondialdehyde by-products) occurred in all the three levels of water stress, although drought represented the major determinant. Drought treatments (75%, 50% and 25% FC) decreased plant height, radial stem diameter, harvest index, total biomass content and RWC in all the three watering regimes compared to control (100% FC). Biochemical parameters like proline, soluble sugar and MDA content increased with severity and duration of stress, which helped plants to survive under severe stress. It was analyzed that for better wood yield poplar seedlings should avail either optimum amount of water (amount nearly equal to field capacity of soil) or maximum withdrawal up to 75% of field capacity up to seedling establishment period (60 days). Furthermore, this study manifested that acclimation to drought stress is related with the rapidity, severity, and duration of the drought event of the poplar species.

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