scholarly journals Enhanced tolerance to drought stress resulting from Caragana korshinskii CkWRKY33 in transgenic Arabidopsis thaliana

2020 ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Water Soluble ◽  
Caragana Korshinskii ◽  
Group I ◽  
Relative Water

Abstract Background: It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.Results: In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative water, soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment.Conclusions: This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

scholarly journals Enhanced Tolerance to Drought Stress Resulting from Caragana Korshinskii CkWRKY33 in Transgenic Arabidopsis Thaliana

2020 ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Water Soluble ◽  
Caragana Korshinskii ◽  
Group I ◽  
Relative Water

Abstract Background: It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses.Results: In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative water, soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment. Conclusions: This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

scholarly journals Enhanced tolerance to drought stress resulting from Caragana korshinskii CkWRKY33 in transgenic Arabidopsis thaliana

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zhen Li ◽  
Fengping Liang ◽  
Tianbao Zhang ◽  
Na Fu ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Responses ◽  
Soluble Sugar ◽  
Survival Rates ◽  
Wrky Transcription Factor ◽  
Wild Type ◽  
Sequence Structure ◽  
Caragana Korshinskii ◽  
Group I

Abstract Background It is well known that WRKY transcription factors play important roles in plant growth and development, defense regulation and stress responses. Results In this study, a WRKY transcription factor, WRKY33, was cloned from Caragana korshinskii. A sequence structure analysis showed that it belonged to the Group-I type. Subcellular localization experiments in tobacco epidermal cells showed the presence of CkWRKY33 in the nucleus. Additionally, CkWRKY33 was overexpressed in Arabidopsis thaliana. A phenotypic investigation revealed that compared with wild-type plants, CkWRKY33-overexpressing transgenic plants had higher survival rates, as well as relative soluble sugar, proline and peroxidase contents, but lower malondialdehyde contents, following a drought stress treatment. Conclusions This suggested that the overexpression of CkWRKY33 led to an enhanced drought-stress tolerance in transgenic A. thaliana. Thus, CkWRKY33 may act as a positive regulator involved in the drought-stress responses in Caragana korshinskii.

Isolation and characterization of MbWRKY1, a WRKY transcription factor gene from Malus baccata (L.) Borkh involved in drought tolerance

2018 ◽  
Vol 98 (5) ◽  
pp. 1023-1034 ◽  
Author(s):  
Deguo Han ◽  
Haibin Ding ◽  
Lijing Chai ◽  
Wei Liu ◽  
Zhaoyuan Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Stress Responses ◽  
Wrky Transcription Factor ◽  
Drought Treatment ◽  
Isolation And Characterization ◽  
Relative Water ◽  
Stress Related Genes ◽  
Malus Baccata

WRKY transcription factors are involved in stress responses in plants; however, their roles in abiotic stresses are still not well known in Malus plants. In the present study, a WRKY gene was isolated from Malus baccata (L.) Borkh and designated as MbWRKY1. Subcellular localization revealed that MbWRKY1 was localized in the nucleus. The expression levels of MbWRKY1 were up-regulated by dehydration, salinity, and ABA treatments in M. baccata seedlings. When MbWRKY1 was introduced into tobacco, it improved drought stress tolerance in transgenic plants. Under the drought treatment, transgenic plants had higher contents of chlorophyll, proline, relative water, AsA, and GSH than wild-type (WT) plants. Compared with WT plants, the overexpression of MbWRKY1 in transgenic tobacco also led to decreased levels of H2O2, MDA, and elecrolyte leakage when dealing with drought stress. There were increased activities of POD, CAT, SOD, and APX in transgenic tobaccos, especially when dealing with drought treatment. Moreover, the MbWRKY1 transgenic plants enhanced the expressions of oxidative stress response (NtPOD, NtCAT, NtSOD, and NtAPX) and stress-related genes (NtP5CS and NtLEA5) when dealing with drought stress. These results suggest that the MbWRKY1 gene plays a positive regulatory role in drought stress response.

scholarly journals Water-soluble chlorophyll protein is involved in herbivore resistance activation during greening of Arabidopsis thaliana

2015 ◽  
Vol 112 (23) ◽  
pp. 7303-7308 ◽  
Author(s):  
Edouard Boex-Fontvieille ◽  
Sachin Rustgi ◽  
Diter von Wettstein ◽  
Steffen Reinbothe ◽  
Christiane Reinbothe
Keyword(s):  
Arabidopsis Thaliana ◽  
Stress Responses ◽  
Fluorescent Protein ◽  
Seedling Emergence ◽  
Water Soluble ◽  
Herbivore Resistance ◽  
Porcellio Scaber ◽  
Apical Hook ◽  
Etiolated Plants

Water-soluble chlorophyll proteins (WSCPs) constitute a small family of unusual chlorophyll (Chl)-binding proteins that possess a Kunitz-type protease inhibitor domain. In Arabidopsis thaliana, a WSCP has been identified, named AtWSCP, that forms complexes with Chl and the Chl precursor chlorophyllide (Chlide) in vitro. AtWSCP exhibits a quite unexpected expression pattern for a Chl binding protein and accumulated to high levels in the apical hook of etiolated plants. AtWSCP expression was negatively light-regulated. Transgenic expression of AtWSCP fused to green fluorescent protein (GFP) revealed that AtWSCP is localized to cell walls/apoplastic spaces. Biochemical assays identified AtWSCP as interacting with RD21 (RESPONSIVE TO DESICCATION 21), a granulin domain-containing cysteine protease implicated in stress responses and defense. Reconstitution experiments showed tight interactions between RD21 and WSCP that were relieved upon Chlide binding. Laboratory feeding experiments with two herbivorous isopod crustaceans, Porcellio scaber (woodlouse) and Armadillidium vulgare (pillbug), identified the apical hook as Achilles’ heel of etiolated plants and that this was protected by RD21 during greening. Because Chlide is formed in the apical hook during seedling emergence from the soil, our data suggest an unprecedented mechanism of herbivore resistance activation that is triggered by light and involves AtWSCP.

scholarly journals Leaf Age-Dependent Photosystem II Photochemistry and Oxidative Stress Responses to Drought Stress in Arabidopsis thaliana Are Modulated by Flavonoid Accumulation

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4157
Author(s):  
Ilektra Sperdouli ◽  
Julietta Moustaka ◽  
Georgia Ouzounidou ◽  
Michael Moustakas
Keyword(s):  
Lipid Peroxidation ◽  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Photosystem Ii ◽  
Stress Responses ◽  
Heat Dissipation ◽  
Leaf Age ◽  
Psii Photochemistry ◽  
Young Leaves ◽  
Flavonoid Accumulation

We investigated flavonoid accumulation and lipid peroxidation in young leaves (YL) and mature leaves (ML) of Arabidopsis thaliana plants, whose watering stopped 24 h before sampling, characterized as onset of drought stress (OnDS), six days before sampling, characterized as mild drought stress (MiDS), and ten days before sampling, characterized as moderate drought stress (MoDS). The response to drought stress (DS) of photosystem II (PSII) photochemistry, in both leaf types, was evaluated by estimating the allocation of absorbed light to photochemistry (ΦPSII), to heat dissipation by regulated non-photochemical energy loss (ΦNPQ) and to non-regulated energy dissipated in PSII (ΦNO). Young leaves were better protected at MoDS than ML leaves, by having higher concentration of flavonoids that promote acclimation of YL PSII photochemistry to MoDS, showing lower lipid peroxidation and excitation pressure (1 − qp). Young leaves at MoDS possessed lower 1 − qp values and lower excess excitation energy (EXC), not only compared to MoDS ML, but even to MiDS YL. They also possessed a higher capacity to maintain low ΦNO, suggesting a lower singlet oxygen (1O2) generation. Our results highlight that leaves of different developmental stage may display different responses to DS, due to differential accumulation of metabolites, and imply that PSII photochemistry in Arabidopsis thaliana may not show a dose dependent DS response.

scholarly journals Physiological and Biochemical Responses of Two Herbaceous Peony Cultivars to Drought Stress

HortScience ◽  
2019 ◽  
Vol 54 (3) ◽  
pp. 492-498 ◽  
Author(s):  
Qi Wang ◽  
Rui Zhao ◽  
Qihang Chen ◽  
Jaime A. Teixeira da Silva ◽  
Liqi Chen ◽  
...  
Keyword(s):  
Drought Stress ◽  
Soluble Protein ◽  
Soluble Sugar ◽  
Damage Index ◽  
Principal Component ◽  
Flowering Plant ◽  
Herbaceous Peony ◽  
Biochemical Responses ◽  
Relative Water ◽  
Physiological And Biochemical

Herbaceous peony is a perennial flowering plant with strong environmental adaptability and may be a good candidate for culture in arid areas. In this study, the physiological and biochemical responses of two herbaceous peony cultivars to different soil moisture gradients in pots were assessed by analyzing changes in 13 stress-related indices. The drought damage index (DDI) and the contents of malondialdehyde (MDA), soluble sugar, proline, and abscisic acid (ABA) generally increased as drought stress intensified, whereas leaf relative water content (LRWC) decreased, and the contents of soluble protein, indole-3-acetic acid (IAA), the ratio of IAA and ABA, and the activities of four antioxidant enzymes fluctuated. For the leaves, a positive correlation was found between DDI and superoxide dismutase (SOD), MDA, soluble sugar, proline, ascorbate peroxidase (APX), and ABA, but it was negatively correlated with LRWC, peroxidase (POD), and catalase (CAT). In fibrous roots, DDI was positively correlated with MDA, soluble sugar, proline, soluble protein, and ABA but was negatively correlated with SOD, CAT, APX, and IAA/ABA. Principal component analysis and subordinate functions were used to evaluate drought resistance of the two cultivars, with ‘Karl Rosenfield’ showing greater resistance to drought than ‘Da Fu Gui’.

Use of DArT molecular markers for QTL analysis of drought-stress responses in soybean. I. Phenotypic evaluation of traits

2015 ◽  
Vol 66 (8) ◽  
pp. 802 ◽  
Author(s):  
Hang T. T. Vu ◽  
A. T. James ◽  
R. J. Lawn ◽  
L. M. Bielig ◽  
A. Kilian
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Recombinant Inbred Lines ◽  
Companion Paper ◽  
Severe Drought ◽  
Dart Markers ◽  
Phenotypic Data ◽  
Relative Water ◽  
Trait Locus

Physiological drought stress responses were assessed in recombinant inbred lines (RILs) from three soybean (Glycine max (L.) Merr.) crosses, in preparation for quantitative trait locus (QTL) analyses using Diversity Arrays Technology (DArT) markers. The three RIL populations were derived from pairwise crosses between three genotypes, cv. Valder, CPI 26671 and G2120, which in previous studies had differed in drought-stress response. Of particular interest was the landrace variety G2120, which in the previous reports had recovered better after severe drought. To assess drought-stress response, the plants were grown in deep cylindrical pots in the glasshouse and exposed to severe water deficit followed by re-watering. Two plants to be genotyped were grown in each pot, together with one plant of G2120, which served as a reference plant against which the responses of the two other plants were assessed. Traits recorded included measures of relative water content (RWC), epidermal conductance (ge) and recovery in growth following re-watering. The responses in the reference and parental plants and the RIL populations were broadly consistent with previous studies. As plant-available water in the soil declined, both RWC and ge declined, although the relation between RWC and ge was exponential, rather than linear as in previous studies. Analysis of variance revealed large environmental effects on most of the traits, which resulted in high coefficients of variation and low estimates of broad-sense heritability. However, there were significant differences at both the population and genotype levels for all key traits, confirming the presence of genetic variation for drought-stress response. Some opportunities for enhancing the observed genetic differences and reducing the environmental noise in future studies are canvassed. Application of the observed phenotypic data reported in this paper in subsequent QTL analyses based on DArT markers is reported in the companion paper.

scholarly journals The WRKY Transcription Factor Genes inLotus japonicus

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Hui Song ◽  
Pengfei Wang ◽  
Zhibiao Nan ◽  
Xingjun Wang
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Gene Clusters ◽  
Wrky Transcription Factor ◽  
Whole Genome Sequence ◽  
Gene Copy ◽  
Group I ◽  
A Genome ◽  
Transcription Factor Genes ◽  
Group Ii

WRKY transcription factor genes play critical roles in plant growth and development, as well as stress responses. WRKY genes have been examined in various higher plants, but they have not been characterized inLotus japonicus. The recent release of theL. japonicuswhole genome sequence provides an opportunity for a genome wide analysis of WRKY genes in this species. In this study, we identified 61 WRKY genes in theL. japonicusgenome. Based on the WRKY protein structure,L. japonicusWRKY (LjWRKY) genes can be classified into three groups (I–III). Investigations of gene copy number and gene clusters indicate that only one gene duplication event occurred on chromosome 4 and no clustered genes were detected on chromosomes 3 or 6. Researchers previously believed that group II and III WRKY domains were derived from the C-terminal WRKY domain of group I. Our results suggest that some WRKY genes in group II originated from the N-terminal domain of group I WRKY genes. Additional evidence to support this hypothesis was obtained byMedicago truncatulaWRKY (MtWRKY) protein motif analysis. We found that LjWRKY and MtWRKY group III genes are under purifying selection, suggesting that WRKY genes will become increasingly structured and functionally conserved.

scholarly journals Genome-wide identification and expression analysis of the WRKY transcription factor family in flax (Linum usitatissimum L.)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hongmei Yuan ◽  
Wendong Guo ◽  
Lijuan Zhao ◽  
Ying Yu ◽  
Si Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Plant Growth ◽  
Stress Responses ◽  
Linum Usitatissimum ◽  
Developmental Stages ◽  
Lignin Content ◽  
Expression Patterns ◽  
Wrky Transcription Factor ◽  
Group I ◽  
Genome Wide

Abstract Background Members of the WRKY protein family, one of the largest transcription factor families in plants, are involved in plant growth and development, signal transduction, senescence, and stress resistance. However, little information is available about WRKY transcription factors in flax (Linum usitatissimum L.). Results In this study, comprehensive genome-wide characterization of the flax WRKY gene family was conducted that led to prediction of 102 LuWRKY genes. Based on bioinformatics-based predictions of structural and phylogenetic features of encoded LuWRKY proteins, 95 LuWRKYs were classified into three main groups (Group I, II, and III); Group II LuWRKYs were further assigned to five subgroups (IIa-e), while seven unique LuWRKYs (LuWRKYs 96–102) could not be assigned to any group. Most LuWRKY proteins within a given subgroup shared similar motif compositions, while a high degree of motif composition variability was apparent between subgroups. Using RNA-seq data, expression patterns of the 102 predicted LuWRKY genes were also investigated. Expression profiling data demonstrated that most genes associated with cellulose, hemicellulose, or lignin content were predominantly expressed in stems, roots, and less in leaves. However, most genes associated with stress responses were predominantly expressed in leaves and exhibited distinctly higher expression levels in developmental stages 1 and 8 than during other stages. Conclusions Ultimately, the present study provides a comprehensive analysis of predicted flax WRKY family genes to guide future investigations to reveal functions of LuWRKY proteins during plant growth, development, and stress responses.

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