scholarly journals Effects of irradiance, temperature, nutrients, and pCO2 on the growth and biochemical composition of cultivated Ulva fenestrata

2020 ◽  
Vol 32 (5) ◽  
pp. 3243-3254 ◽  
Author(s):  
Gunilla B. Toth ◽  
Hanna Harrysson ◽  
Niklas Wahlström ◽  
Joakim Olsson ◽  
Annelous Oerbekke ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Low Temperature ◽  
Biochemical Composition ◽  
Phenolic Content ◽  
Fatty Acid Content ◽  
Algal Species ◽  
Negative Relationship ◽  
Green Algal ◽  
Ulva Fenestrata ◽  
Acid Protein

Abstract Ulva fenestrata is an economically and ecologically important green algal species with a large potential in seaweed aquaculture due to its high productivity, wide environmental tolerance, as well as interesting functional and nutritional properties. Here, we performed a series of manipulative cultivation experiments in order to investigate the effects of irradiance (50, 100, and 160 μmol photons m−2 s−1), temperature (13 and 18 °C), nitrate (< 5, 150, and 500 μM), phosphate (< 1 and 50 μM), and pCO2 (200, 400, and 2500 ppm) on the relative growth rate and biochemical composition (fatty acid, protein, phenolic, ash, and biochar content) in indoor tank cultivation of Swedish U. fenestrata. High irradiance and low temperature were optimal for the growth of this northern hemisphere U. fenestrata strain, but addition of nutrients or changes in pCO2 levels were not necessary to increase growth. Low irradiance resulted in the highest fatty acid, protein, and phenolic content, while low temperature had a negative effect on the fatty acid content but a positive effect on the protein content. Addition of nutrients (especially nitrate) increased the fatty acid, protein, and phenolic content. High nitrate levels decreased the total ash content of the seaweeds. The char content of the seaweeds did not change in response to any of the manipulated factors, and the only significant effect of changes in pCO2 was a negative relationship with phenolic content. We conclude that the optimal cultivation conditions for Swedish U. fenestrata are dependent on the desired biomass traits (biomass yield or biochemical composition).

scholarly journals Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

2021 ◽  
Vol 9 (6) ◽  
pp. 615
Author(s):  
Sophie Steinhagen ◽  
Swantje Enge ◽  
Karin Larsson ◽  
Joakim Olsson ◽  
Göran M. Nylund ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Low Temperature ◽  
Northern Hemisphere ◽  
Biochemical Composition ◽  
Large Scale ◽  
Biomass Yield ◽  
Phenolic Content ◽  
Seedling Density ◽  
Ulva Fenestrata ◽  
High Nutrient

The growing world population demands an increase in sustainable resources for biorefining. The opening of new farm grounds and the cultivation of extractive species, such as marine seaweeds, increases worldwide, aiming to provide renewable biomass for food and non-food applications. The potential for European large-scale open ocean farming of the commercial green seaweed crop Ulva is not yet fully realized. Here we conducted manipulative cultivation experiments in order to investigate the effects of hatchery temperature (10 and 15 °C), nutrient addition (PES and 3xPES) and swarmer density (500 and 10,000 swarmers ml−1) on the biomass yield and biochemical composition (fatty acid, protein, carbohydrate, pigment and phenolic content) of off-shore cultivated Ulva fenestrata in a Swedish seafarm. High seedling densities were optimal for the growth of this northern hemisphere crop strain and significantly increased the mean biomass yield by ~84% compared to low seedling densities. Variations of nutrients or changes in temperature levels during the hatchery phase were not necessary to increase the subsequent growth in an open-water seafarm, however effects of the factors on the thallus habitus (thallus length/width) were observed. We found no significant effect of the environmental factors applied in the hatchery on the total fatty acid or crude protein content in the off-shore cultivated Ulva. However, low seedling density and low temperature increased the total carbohydrate content and furthermore, high temperature in combination with high nutrient levels decreased the pigment content (chlorophyll a, b, carotenoids). Low temperature in combination with high nutrient levels increased the phenolic content. Our study confirms the successful and sustainable potential for large-scale off-shore cultivation of the Scandinavian crop U. fenestrata. We conclude that high seedling density in the hatchery is most important for increasing the total biomass yield of sea-farmed U. fenestrata, and that changing temperature or addition of nutrients overall does not have a large effect on the biochemical composition. To summarize, our study contributes novel insights into the large-scale off-shore cultivation potential of northern hemisphere U. fenestrata and underpins suitable pre-treatments during the hatchery phase of seedlings to facilitate a successful and cost-efficient large-scale rope cultivation.

scholarly journals Prediction of microalgae hydrothermal liquefaction products from feedstock biochemical composition

2015 ◽  
Vol 17 (6) ◽  
pp. 3584-3599 ◽  
Author(s):  
Shijie Leow ◽  
John R. Witter ◽  
Derek R. Vardon ◽  
Brajendra K. Sharma ◽  
Jeremy S. Guest ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Content ◽  
Biochemical Composition ◽  
Fatty Acid Content ◽  
Hydrothermal Liquefaction

Models for microalgae hydrothermal liquefaction were developed from conversion of Nannochloropsis cultivated to varying biochemical composition and fatty acid content.

Chemical components and biological activities of two freshwater green algae from Ordu, Turkey / Türkiye’nin Ordu ilinden iki tatlı su yeşil alglerinin kimyasal bileşenleri ve biyolojik aktiviteleri

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
Beyhan Taş ◽  
Ömer Ertürk ◽  
Özlem Yılmaz ◽  
Melek Çol Ayvaz ◽  
Emine Yurdakul Ertürk
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Activities ◽  
Algal Species ◽  
Acetone Extract ◽  
Diethyl Ester ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Green Algal

AbstractObjective: Scientists are looking for new resources which have biological activities. The present study was planned to evaluate the antioxidant and antimicrobial activity of ethanol and acetone extracts, as well as the volatile compounds of two freshwater green algal species Spirogyra spp. and Zygnema stellinum (Vaucher) C. Agardh belonging to Zygnemaphyceae (Conjugatophyceae) obtained from Ordu University Campus wetlands.Methods: The extracts were tested in vitro for their antimicrobial effects using disc diffusion method. Total phenolic content and the antioxidative activity according to FRAP and DPPH methods of the extracts were also determined. The secondary metabolites from the investigated extracts were identified using GC-MS.Results: The extracts dramatically inhibited almost all tested microorganisms. The antimicrobial activity of the acetone extract of the Zygnema against C. albicans was found as more higher than positive control Nystatin. In accordance with antimicrobial activity, the highest total phenolic content was also determined in the presence of the acetone extract of the Zygnema. Furthermore the highest FRAP value and the lowest EC50 (mg/mL) value were calculated for the same extract. The main components of the all consisted of dimethyl and diethyl ester of 1,2-benzenedicarboxylic acid. However, the abundance of these metabolites in the extracts was not associated with antimicrobial or antioxidant activity. Biological activities of these algal species could be attributed to chemicals such as 1-Pentadecene and 1-Tetradecene which were present in smaller amounts.Conclusion: Investigated algal species can be evaluated to use in biotechnological applications such as food industry and medicine.

scholarly journals The biochemical content and antioxidant capacities of endemic Tanacetum densum (Lab.) Schultz Bip. subsp. laxum, and Tanacetum densum (Lab.) Schultz Bip. subsp. amani Heywood growing in Turkey

2021 ◽  
Vol 81 (4) ◽  
pp. 1106-1114
Author(s):  
İ. Emre
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Fatty Acid Content ◽  
Radical Scavenging ◽  
Unsaturated Fatty Acid Content

Abstract Medicinal plants have a significant role in preventing and curing several diseases, and Tanacetum L. is one of these plants. The aim of the present study is to determine the fatty acid, lipid-soluble vitamin, sterol, phenolic content, and antioxidant capacity of Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani, to compare the effect of altitude on the biochemical content and to compare systematically by using fatty acids and phenolics. This study showed that palmitic acid (C16:0) and stearic acid (C18:0) are major sources of saturated fatty acid and oleic acid (C18:1 n9), and linoleic acid (18:2 n6c) and a-linolenic acid (C18:3 n3) are the principal unsaturated fatty acids in the two endemic Tanacetum densum taxa. Also, this study found that the unsaturated fatty acid content (60.11±1.61%) of Tanacetum densum subsp. laxum was higher than the unsaturated fatty acid content (44.13±1.28%) of Tanacetum densum subsp. amani. And also, the ω6/ω3 ratio of Tanacetum densum subsp. laxum (1.74) and Tanacetum densum subsp. amani (1.60) was found to be similar. However, this study determined that the lipid soluble vitamin and sterol content of two endemic Tanacetum taxa are low except for stigmasterol. Present study showed that catechin is principal phenolic in the Tanacetum densum taxa. This study also found that Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani had the highest levels of catechin, vanillic acid, and caffeic acid content though the phenolic amounts, particularly catechin and quercetin, were dissimilar in the T. densum taxa. This study suggested that ecological conditions such as altitude may affect the biochemical content of two endemic Tanacetum densum taxa. Furthermore, the current study determined that two endemic Tanacetum L. taxa had potent radical scavenging capacities and found a correlation between total phenolics and antioxidant activity.

scholarly journals Selection of Indigenous Algal Species for Potential Biodiesel Production

2021 ◽  
Author(s):  
Vinaya Tiwari ◽  
Alok Das ◽  
Shallu Thakur ◽  
Rakesh Kumar Trivedi
Keyword(s):  
Fatty Acid ◽  
Fossil Fuels ◽  
Algal Species ◽  
Fuel Properties ◽  
Biodiesel Production ◽  
Fatty Acid Profiles ◽  
Algal Strain ◽  
Green Algal ◽  
Carbon Neutral ◽  
Selection Of

Currently, India utilizes an enormous amount of fossil fuels and a major quantity of fossil fuels are imported from other countries. It’s a giant load on the Indian Economy. The burning of fossil fuels causes global warming. Carbon neutral, renewable fuels are essential for environmental protection and it’s economically sustainable for India. Biofuels attention day by day due to a rise in energy demands and environmental concerns. Biodiesel produced from algal oil a possible renewable and carbon-neutral substitute to fossil fuels. The feasibility of the algal-based biodiesel industry depends on the selection of adequate species regarding commercial oil yields and oil quality. Present research work to bioprospecting and screening of 19 algal and blue-green algal species, the oil percentage and the fatty acid profiles, used for analyzing the biodiesel fuel properties. Oil from Tolypothrix phyllophila algal strain and compared it with another eighteen algal and blue-green algal strains from different literature. Tolypothrix phyllophila algal strain contains approximately 12.6% lipid on a dry weight basis. We also compared the FAME profile of 19 algal and blue-green algal strains and calculated and compared the fuel properties such as cetane number, Iodine Value, etc. of the biodiesel derived from these algal and blue-green algal oils based on chain length and saturation. We also investigated the 19 algal and blue-green algal fatty acid profiles and its suitability for biodiesel production and strains selection through PROMETHEE (Preference Ranking Organization Method for Enrichment Evaluations) and GAIA (geometrical analysis for interactive aid) analysis.

Effect of cultivar and fungicide treatment on the yield and nutritive value of winter and spring oat grains grown in England and Wales, 1989–91

1998 ◽  
Vol 130 (4) ◽  
pp. 411-421 ◽  
Author(s):  
P. M. HAIGH ◽  
N. J. BRADSHAW
Keyword(s):  
Fatty Acid ◽  
Grain Yield ◽  
Nutritive Value ◽  
Fatty Acid Content ◽  
Specific Weight ◽  
The Other ◽  
Distinct Advantage ◽  
Fungicide Treatment ◽  
Acid Protein ◽  
Naked Oats

The effect of a comprehensive (three-spray) fungicide programme on yield, specific weight (SW), 1000-grain weight (TGW), nutritive value and yield of both winter and spring oat grains was investigated over a 3-year period (1989–91). With winter oats, four cultivars (Aintree, Image or Pennal, Solva and Kynon), were tested at each of three sites (Gwent, Hereford and Kent). Similarly for spring oats, four cultivars (Dula, Keeper, Rollo and Rhiannon) were tested at each of three sites (Cornwall, North Yorkshire and Wales).On winter oats, fungicide treatment produced a significant increase (P<0·05) in grain yield, but did not on spring oats. Fungicide treatment produced no significant effect on SW, TGW or nutritive value of the winter or spring oats. With winter oats, but not with spring oats, fungicide treatment produced a significant increase (P<0·05) in the yield of digestible (DE) and metabolizable (ME) energy for pigs and ruminants respectively.The naked oats, Kynon and Rhiannon, had a significantly lower yield, higher SW and lower TGW than the other conventional cultivars (P<0·05). Their oil, fatty acid, protein, DE and ME contents were also significantly higher than the other cultivars (P<0·05). Nevertheless, the yield of protein, DE and ME tended to be less from naked oats than from some of the other cultivars tested.On winter oats, TGW, protein content and protein yield, dry matter (DM) and fatty acid content and on spring oats, yield, TGW, ME yield, DM, oil, fatty acid, protein, DE and ME all varied between years. With winter oats, oil content and energy values were negatively correlated with yield and positively correlated with SW. With spring oats, DE and ME were positively correlated with SW. It was concluded that, on winter oats, fungicide treatment was worthwhile in terms of grain yield, but this was not the case for spring oats. Also with winter oats, but not spring oats, fungicides gave useful increases in the yield of energy. The naked oats, Kynon and Rhiannon, had a distinct advantage in terms of nutritive value, but their low yield compared with the conventional cultivars tended to negate this benefit.

scholarly journals Portuguese Kelps: Feedstock Assessment for the Food Industry

2021 ◽  
Vol 11 (22) ◽  
pp. 10681
Author(s):  
Diana Pacheco ◽  
Giuseppe Miranda ◽  
Carolina P. Rocha ◽  
Rosinda L. Pato ◽  
João Cotas ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Total Phenolic Content ◽  
Unsaturated Fatty Acids ◽  
Phenolic Content ◽  
Fatty Acid Content ◽  
Saccharina Latissima ◽  
Total Phenolic ◽  
Composition Analysis ◽  
Flame Atomic Absorption ◽  
Soxhlet Apparatus

Seaweeds have been incorporated in the daily diet of several human cultures since ancient times, due to their nutritional characteristics and healthy properties. The brown seaweeds Undaria pinnatifida, Saccharina latissima, Sacchoriza polyschides, and Laminaria ochroleuca were collected in the Viana do Castelo (Portugal) bay to assess their proximate composition analysis. As a result, the algal biomass was dried, and its moisture and ash content were determined. The dried biomass was then analyzed for total nitrogen/total protein (using the Kjeldahl method), total fiber content (through fiber analyzer digestion), total lipids (in a Soxhlet apparatus), and fatty acid characterization (by gas chromatography-mass spectrometry). Apart from phosphorus, which was analyzed by spectrophotometry, the ashes were employed for mineral and trace element characterization via dry mineralization and quantified using flame atomic absorption spectrometry. Moreover, the total phenolic content was assessed spectrophotometrically by the Folin-Ciocalteu method in the algal aqueous extracts. Analyses showed that their protein concentrations ranged from 12 to 24% dry weight (DW), while lipid concentrations varied between 0.51% and 1.52% DW. Regarding the carbohydrate concentration in these seaweeds, a concentration between 48% and 60% DW was observed. The S. polyschides had the highest overall total phenolic content (6.19 × 10−3 g GAE/100 g of dried algae), while L. ochroleuca had the lowest amount (3.72 × 10−3 g GAE/100 g of dried algae). U. pinnatifida had the highest total fatty acid content (35.13 mg/g DW), whereas S. latissima presented the lowest value (22.59 mg/g DW). Significant concentrations of highly unsaturated fatty acids (HUFA) were observed in both seaweeds, with U. pinnatifida having the highest value (10.20 mg/g DW) and S. latissima the lowest content (4.81 mg/g DW). It is also highlighted that these seaweeds have a nutritional relevance as a source of essential nutrients, including nitrogen, potassium, sodium, calcium, magnesium, and iron.

scholarly journals Analysis of soybean germplasm by seed longevity

2020 ◽  
Vol 27 ◽  
pp. 226-231
Author(s):  
O. A. Zadorozhna ◽  
O. M. Bezugla ◽  
O. N. Vus ◽  
O.G. Suprun ◽  
T.P. Shyianova
Keyword(s):  
Fatty Acid ◽  
Moisture Content ◽  
Biochemical Composition ◽  
Fatty Acid Content ◽  
Soybean Seed ◽  
Seed Longevity ◽  
Soybean Seeds ◽  
Aim Analysis ◽  
Seed Moisture Content ◽  
Seed Moisture

Aim. Analysis of soybean (Glycine max (L.) Merr.) germplasm seed longevity with different biochemical composition for further storage optimization in active collections and during long-term storage. Methods. The content of protein, oil, fatty acid composition (palmitic, palmitic-oleic, stearic, oleic, linoleic, linolenic, eicosenic, behenic) in experimental soybean seed samples was analyzed. The process of accelerated storage aging, storage at –20°C were carried out at original seed moisture content and after additional drying. Laboratory and field indexes of seed germinability, mophophysiological state of seedlings, elements of crop structure were evaluated. Results. The longevity of soybean seed germplasm with different biochemical composition, different degree of drying was determined. The statistical relationship between fatty acid content and germination of soybean seeds in experimental and control variants was established. Conclusions. Drying to 4% seed moisture content extends the longevity of soybean seeds with different biochemical composition. Extra drying should be applied in special modes even for seeds with 7% moisture content. Keywords: germplasm, soybean, storage, longevity, moisture content.

scholarly journals Influence of Ice Encasement and Ethylene Regulation on Cellular-protection Responses in Annual Bluegrass

2021 ◽  
pp. 1-12
Author(s):  
Kevin Laskowski ◽  
Emily Merewitz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Low Temperature ◽  
Unsaturated Fatty Acid ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Plant Tissues ◽  
Annual Bluegrass ◽  
Ice Encasement

Annual bluegrass (Poa annua var. reptans), when grown as a putting green species, is sensitive to winter injury such as ice cover. Inhibiting plant ethylene production could be a way to improve annual bluegrass tolerance of ice encasement. The goals of this study were to determine how winter conditions and ethylene regulatory treatments affect the antioxidant system, fatty acid composition, and apoplastic proteins of annual bluegrass plant tissues. Ethylene-promotive (1-aminocyclopropane-1-carboxylic acid or ethephon) and ethylene inhibition treatments [aminoethoxyvinylglycine (AVG)] were applied to plants in the field during acclimation. Plant plugs were taken and subjected to low temperature (−4 °C) and ice-encasement treatments in growth chamber conditions. Antioxidant activities of ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) were measured along with malondialdehyde content (MDA) and apoplastic protein content in leaf and crown tissue. Saturated and unsaturated fatty acid contents were measured in leaf, crown, and root tissue. Higher unsaturated fatty acids are often associated with greater low-temperature tolerance. Compared with the untreated controls, ethephon-treated annual bluegrass had greater MDA contents, lower POD and SOD activity, and greater saturated and decreased unsaturated fatty acids. Ethylene inhibition treatments caused annual bluegrass to have less saturated fatty acid content and greater unsaturated fatty acid content, a greater content of apoplast proteins, and higher CAT activity when compared with the untreated controls. The activity of APX was greater in AVG-treated annual bluegrass than in controls. Ethylene may reduce physiological health overwinter, and inhibitory treatments may promote winter tolerance by promoting antioxidant activity, apoplast proteins, and the content of unsaturated fatty acids in plant tissues.

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