New Synthetic Peptides Conjugated to Gold Nanoclusters: Antibiotic Activity Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

2019 ◽  
Vol 38 (5) ◽  
pp. 506-514
Author(s):  
Y. A. Prada ◽  
Fanny Guzmán ◽  
Claudia Ortíz ◽  
Rafael Cabanzo ◽  
Rodrigo Torres ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Synthetic Peptides ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Gold Nanoclusters ◽  
Antibiotic Activity ◽  
Escherichia Coli O157 ◽  
Methicillin Resistant

scholarly journals Antibacterial effects of wood structural components and extractives from Pinus sylvestris and Picea abies on methicillin-resistant Staphylococcus aureus and Escherichia coli O157:H7

BioResources ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. 7601-7614 ◽  
Author(s):  
Tiina Vainio-Kaila ◽  
Xue Zhang ◽  
Tuomas Hänninen ◽  
Aino Kyyhkynen ◽  
Leena-Sisko Johansson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Photoelectron Spectroscopy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Escherichia Coli O157 ◽  
Structural Components ◽  
Methicillin Resistant ◽  
Viable Bacteria

Antibacterial properties of wood structural components and extractives were investigated against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli O157:H7 by placing bacterial inoculum on the model surfaces and incubating them for 2, 4, and 24 h. After incubation, the amount of viable bacteria on the surfaces was studied. The film coverage and thickness were evaluated with atomic-force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The extracts were analyzed with gas chromatography–mass spectrometry (GC-MS). The results showed that films fully covered the glass surfaces. The XPS results confirmed the analysis of GC-MS, which revealed more similarities between the extractives of pine heartwood and spruce heartwood than between pine heartwood and pine sapwood. Only the pine heartwood extract showed an antibacterial effect against E. coli O157:H7. In contrast, MRSA was susceptible to all of the extracts and milled wood lignin (MWL).

scholarly journals Synthesis of antibiotic loaded polylactic acid nanoparticles and their antibacterial activity against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus

Biomédica ◽  
2017 ◽  
Vol 37 (1) ◽  
pp. 11 ◽  
Author(s):  
Mónica Tatiana Herrera ◽  
Jhon Jhamilton Artunduaga ◽  
Claudia Cristina Ortiz ◽  
Rodrigo Gonzalo Torres
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Polylactic Acid ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Escherichia Coli O157 ◽  
Methicillin Resistant ◽  
E Coli

Introducción. Las nanopartículas poliméricas constituyen una herramienta nanotecnológica que podría ayudar a combatir los microorganismos patógenos que han desarrollado resistencia a los antibióticos convencionales.Objetivo. Sintetizar nanopartículas de ácido poliláctico cargadas con ofloxacina y vancomicina, y determinar su actividad antibacteriana frente a Escherichia coli O157:H7 y Staphylococcus aureus resistente a la meticilina (SARM).Materiales y métodos. Las nanopartículas de ácido poliláctico cargadas con ofloxacina y vancomicina se sintetizaron utilizando el método de emulsión y evaporación de solvente. Se caracterizaron mediante dispersión de luz en modo dinámico, electroforesis Doppler con láser y microscopía electrónica de barrido (S-TEM). Se evaluó la actividad antibacteriana in vitro de las nanopartículas de ácido poliláctico con ofloxacina contra E. coli O157:H7 y nanopartículas de ácido poliláctico con vancomicina contra SARM, mediante el método de microdilución en caldo.Resultados. Se obtuvieron nanopartículas poliméricas con tamaños inferiores a 379 nm y carga superficial positiva de hasta 21 mV. Las nanopartículas cargadas con ofloxacina presentaron una concentración inhibitoria mínima (CIM50) de 0,001 μg/ml frente a E. coli O157:H7, valor 40 veces menor que la concentración de antibiótico libre necesaria para lograr el mismo efecto (CIM50=0,04 μg/ml). Para SARM, las nanopartículas mejoraron la potencia farmacológica in vitro de la vancomicina alexhibir una MIC50 de 0,005 μg/ml, comparada con la de 0,5 μg/ml del antibiótico libre.Conclusiones. Se mejoró el efecto antibacteriano de la ofloxacina y la vancomicina incorporadas en la matriz polimérica de ácido poliláctico. Las nanopartículas poliméricas constituirían una alternativa para el control de cepas bacterianas de interés en salud pública.

scholarly journals PREPARATION OF COLD CREAM AGAINST CLINICAL PATHOGEN USING CARALLUMA ADSCENDENS VAR. ATTENUATA

2020 ◽  
pp. 120-123
Author(s):  
SUNDAR MADASAMY ◽  
SURESH SUNDAN ◽  
LINGAKUMAR KRISHNASAMY
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Positive Control ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Cold Cream ◽  
Significant Difference ◽  
Vancomycin Resistant

Objective: A simple formulation of cold cream from methanolic extract Caralluma adscendens var. attenuata (MECA) and their antimicrobial activity was tested against various clinical pathogens, namely, Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, and Candida albicans. Methods: Methanol extract of these plant extract was prepared by the Soxhlet method. We analyzed phytochemical nature of theses plant, and subsequently, a cream was formulated cold-cream C. adscendens var. attenuata (FCA) different concentration such as FCA 50 mg, FCA 100 mg, and FCA 200 mg. In the present study, aimed to the antimicrobial activity of cold cream was measured by agar well diffusion method, and standard antibiotic Neosporin (market available) cream was used as positive control and dummy cold cream (without-MECA) were used as the negative control. Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. For more, the efficacy of cold cream from MECA extracts to against the clinical pathogen. Positive control Neosporin and 200 mg FCA cream was a highly significant difference in the zone of inhibition when compared to dummy cream. The 200 mg FCA was activity against Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant E. faecium, and C. albicans highly significantly difference (p<0.05) compared FCA 50 mg and FAC 100 mg creams. Conclusion: The results from this study suggested that the cold cream form base of MECA crude had antimicrobial activity in the different clinical pathogen. They could be used as an alternative source to conventional antimicrobial agents for the treatment of pathological infection.

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