A Novel Effective Formulation of Bioactive Compounds for Wound Healing: Preparation, In Vivo Characterization, and Comparison of Various Postbiotics Cold Creams in a Rat Model

The wound is a break in the integrity of the skin produced by injury, illness, or operation. Wound healing is an essential dynamic biological/physiological process that occurs in response to tissue damage. The huge health, economic, and social effects of wounds on patients and societies necessitate the research to find novel potential therapeutic agents in order to promote wound healing. Postbiotics, the newest member of the biotics family, are valuable functional bioactive substances produced by probiotics through their metabolic activity, which have several beneficial properties, including immunomodulatory, anti-inflammatory, antimicrobial, and angiogenesis characteristics, resulting in acceleration of wound healing. In the current study, three topical cold cream formulations containing postbiotics obtained from Lactobacillus fermentum, Lactobacillus reuteri, or Bacillus subtilis sp. natto probiotic strains were prepared. The effectiveness and wound healing activity of the developed postbiotics cold cream formulations were investigated compared to cold cream without postbiotics and no treatment via wound closure investigation, hydroxyproline content assay, and histological assessment in 25 Sprague Dawley rats divided into five groups. Interestingly, analysis of the results revealed that all three formulations containing postbiotics significantly accelerated the wound healing process. However, in general, the Bacillus subtilis natto cold cream manifested a better wound healing property. The pleasing wound healing characteristics of the topical postbiotics cold creams through the in vivo experiment suggest that formulations containing postbiotics can be considered as a promising nominee for wound healing approaches.

Wound-Healing Potential of Cucurbita moschata Duchesne Fruit Peel Extract in a Rat Model of Excision Wound Repair

Background and Aim. Pumpkin (Cucurbita moschata Duchesne) is a medicinal plant with different pharmacological effects such as antioxidant, antidiabetic, hepatoprotective, and anticancer effects. In the present study, we aimed to investigate wound-healing activity of pumpkin fruit peel in a rat model of excision wound repair. Materials and Methods. Hydroalcoholic extractions of pumpkin fruit peel were obtained and used to prepare two different cold cream-based formulations, namely, 10% and 20% pumpkin peel extracts (PPEs). These formulations, phenytoin cream, and cold cream were topically used once daily for 14 days to compare their wound-healing effects in a rat model of excision wound repair. Wound sizes were monitored at different intervals. Skin tissue samples were subject to H&E staining for histopathological analysis. Blood samples were also taken on day 14 to measure serum levels of nitrite. Results. Both 10% and 20% PPE formulations resulted in a significant reduction of wound sizes compared to positive and negative controls. Wound closure rate was estimated to be higher in 20% PPE-treated rats. According to histopathological analysis, treatment with 20% PPE improved parameters associated with efficient wound repair, including better regeneration of epidemic layer, higher density of dermis collagen fibers, and lower presence of inflammatory cells. Also, both formulations lowered serum concentrations of nitrite. Conclusion. Given the obtained data from our study, the hydroalcoholic extract of Cucurbita moschata Duchesne fruit peel is proposed to be effective in accelerating the process of excision wound repair partly due to its antioxidant effect in terms of decreasing nitrite concentration.

A New Approach to Atopic Dermatitis Control with Low-Concentration Propolis-Loaded Cold Cream

Atopic dermatitis (AD) is a chronic inflammatory skin disease that is difficult to treat. Traditional cold cream, a water-in-oil emulsion made from beeswax, is used to alleviate AD symptoms in clinical practice, although its effectiveness has not been scientifically proven. The addition of propolis has the potential to impart anti-inflammatory properties to cold cream. However, in high concentrations, propolis can trigger allergic reactions. Thus, the objective of this work was to develop a cold cream formulation based on purified beeswax containing the same amount of green propolis present in raw beeswax. The impact of adding this low propolis concentration to cold cream on AD control was evaluated in patients compared to cold cream without added propolis (CBlank). Raw beeswax was chemically characterized to define the propolis concentration added to the propolis-loaded cold cream (CPropolis). The creams were characterized as to their physicochemical, mechanical, and rheological characteristics. The effect of CPropolis and CBlank on the quality of life, disease severity, and skin hydration of patients with AD was evaluated in a triple-blind randomized preclinical study. Concentrations of 34 to 120 ng/mL of green propolis extract reduced TNF-α levels in LPS-stimulated macrophage culture. The addition of propolis to cold cream did not change the cream’s rheological, mechanical, or bioadhesive properties. The preclinical study suggested that both creams improved the patient’s quality of life. Furthermore, the use of CPropolis decreased the disease severity compared to CBlank.

Machining the Surface of Orthopedic Stent Wire Using a Non-Toxic Abrasive Compound in a Magnetic Abrasive Finishing Process

The orthopedic stent wire is one of the critical medical components, which is mainly used for the replacement of physically damaged parts in the human body. Therefore, a smooth surface and lack of toxic substances on the surface of this component are highly demanded. In this study, a magnetic abrasive finishing (MAF) process was carried out using a non-toxic abrasive compound (a mixture of iron powder, diamond particles, cold cream, and eco-friendly oils) to achieve high-quality surface finishing of orthopedic stent wire. The surface roughness (Ra) of the stent wire was investigated according to various processing parameters: different rotational speeds (500, 1000, and 2000 rpm), diamond particle sizes (1.0 µm), and three eco-friendly oils (olive oil: C98H184O10; grapeseed oil: C18H32O2; and castor oil: C57H104O9) within 300 s of the finishing time. The results showed that the surface roughness of the wire was reduced to 0.04 µm with a rotation speed of 1000 rpm and a diamond particle size of 1 µm when using grapeseed oil. SEM microimages and EDS analysis showed that the MAF process using a non-toxic abrasive compound could improve the surface quality of orthopedic Ni-Ti stent wire with a lack of toxic substances on the surface finish.

Histological Studies on a Newly Isolated Bacillus subtilis D10 Protease in the Debridement of Burn Wound Eschars Using Mouse Model

Background: Proteases are among the most important industrial enzymes, playing a critical role in the physiological, biochemical, and regulatory processes of all living organisms. This study evaluated the histological effects of a Bacillus subtilis D10 protease in combination with the antibacterial ointment silver sulfadiazine (SSD) on the burned skin of mice. Materials and Methods: The bacterial proteolytic enzyme was produced and purified through DEAE-Sepharose CL-6B and Sephadex G-100 FF. The in vitro protease specificity was then determined. The dorsal skin of albino mice was burned with 80% HCl solution, then treated under three conditions: cold cream, SSD, and SSD combined with the tested protease. After 15 days of daily treatment, the mice were sacrificed and skin tissue samples were histopathologically examined using hematoxylin eosin, and Masson trichrome staining. Results: The D10 protease hydrolyzed the proteinaceous components of eschars (fibrin, normal collagen, and denatured collagen) in vitro. Mice skins treated with protease and SSD mixture showed promising results, with more rapid healing than the other treatments. This group regenerated epidermis and dermis with newly formed granulated follicles, fibroblasts and blood capillaries in the dermis, and collagen fibers in the hypodermis. Conclusions: These results suggest that the serine protease produced by B. subtilis D10 promotes wound healing of mice skin burnt with HCl and restores the normal architectural pattern in a shorter time than the standard treatments.

Cold cream combination of Garcinia mangostana L. Anredera cordifolia (Ten.) and Centella asiatica extracts on Burn Healing Activity Test

Background: Mangosteen peel (Garcinia mangostana)L., binahong leaf (Anredera cordifolia (Ten.)), and centella herb (Centella asiatica) have been shown to have activity in each phase of burn healing process. Formulating these three plants extract into cold cream preparations is expected to increase the healing activity of burns. Objective: This study aimed to prove the efficacy of the cold cream combination of these plants by examining in vivo burn healing activity using a deep second-degree burn rat model. Materials and Methods: Rat were divided into nine experimental groups (normal, saline solution, silver sulfadiazine, cold cream base, each extract, combination of three extracts, and coldcream combination of three extracts). The burn activity was assessed by inflammatory cell, agiogenesis and collagen formation through histopathological examination. Results: Cold cream combination of mangosteen peel, binahong leaf, and centella herb enhanced the burn wound healing as indicated by progressive improvement in wound healing during 21 days after treatment. Histological result showed that coldcream combination group can reduced inflammatory cell infiltration and increased collagen significantly (p ≤ 0.05), in the other hand same as normal control, but has no effect on angiogenesis. Conclusion: Based on these results, cold cream combination of mangosteen peel, binahong leaf, and centella herb enhanced has a potential effect as an burn healing agent.

Experience of using an emollient with an anti-inflammatory effect based on ammonium glycyrrhizinate in the complex therapy of patients with atopic dermatitis

Background. Despite the advances achieved in recent years in the treatment of AD, prevention and rehabilitation of patients, who suffering from this disease, belong to the most difficult tasks of practical dermatovenerology. Objective. The aim of this study was to assess the efficacy, safety and tolerability of Reglisam Cream in the complex therapy of patients with atopic dermatitis. Methods. The study involved 48 patients with atopic dermatitis, who were divided into two groups. There were two observation periods for each patient: 28 days the period of exacerbation therapy, and 6 months basic care and prevention of relapses. In exacerbation period all patients used external moisturizers in addition to topical glucocorticosteroids: in group I (study group) patients applied Reglisam Cream to the skin; in group II (control group) cold cream. After the period of exacerbation patients of group I were prescribed basic therapy Reglisam Cream for 6 months, patients of group II cold cream. In each group were assessed the therapeutic efficacy, safety and tolerability of treatment. During the study were conducted registration of adverse events, assessment of the dermatological status with the determination of the intensity of pruritus according to the visual analogue 10-point scale, the calculation of the severity index of the disease and the area of the lesion in eczema / atopic dermatitis (EASI) and the dermatological index of quality of life (DLQI), measurement of skin moisture, general clinical research. Results. In the first 28 days of acute atopic dermatitis period therapy, the EASI index decreased on average by 76% from 20.4 to 4.8 points (p 0,05), in group II by 57% from 19.7 to 8.4 points (p 0,05); the intensity of pruritus after treatment with Reglisam Cream in the dynamics decreased by 70% and amounted to 2.3 points, which was lower than in the comparison group 4.1 points (p 0,05); the level of skin hydration in group I was 36.8 units, during the treatment period increased by 80%, and was higher than in group II 26.7 units. (p 0,05); DLQI in group I decreased by 68%, amounting to 4.7 points, and was almost 2 times less than in group II 9.2 points (p 0.05). In group I for 6 months were registered a statistically lower frequency of exacerbations of atopic dermatitis (28%) than in group II (63%). The average duration of atopic dermatitis remission in group I was 22.4 weeks, for 4 weeks more than in group II (p 0,05). Meanwhile the average duration for the exacerbation period of atopic dermatitis in group I was more than 3.5 times less in comparison with group II; the average EASI in group I did not exceed 6.4 points and was significantly lower than that in group II (p 0,05). Conclusion. The use Reglisam Cream as basic skin care in patients with AD increases the duration of remission and reduces the frequency and duration of relapses of the disease, leads to an improvement the quality of life, a decrease in the intensity of pruritus and a significant increase in the moisture content of the stratum corneum of the epidermis. The obtained clinical results allow us to recommend Reglisam Cream as an effective external agent in the complex therapy of patients with AD.

SKINCARE CREAM PREPARATION AND EVALUATION OF PARE (MOMORDICA CHARANTIA) LEAVES USING THREE DIFFERENCE BASE

Objective: The purpose of this research is to make a skincare cream that contains bioactive from Momordica charantia leaves (MCL) using three kinds of base cream. Methods: The dried MCL made into powder shape and then extracted using 96% ethanol solvent. The liquid extract was evaporated using a vacuum evaporator until obtained by the viscous extract. The Momordica charantia leaves ethanol extract (MEE) with a concentration of 4.00% made into a cosmetic product used vanishing, cold, and hand and body cream as a base. Creams made by mixing the oil phase and water phase at 70 °C until cream mass formed, then added MEE at 40 °C. All the product creams were evaluated their physical and chemical characteristics and stability tested. Results: Cream of Momordica charantia leaf ethanol extract had semisolid form, yellowish color, the peculiar smell of Momordica charantia ethanol extract, homogeneous, pH 6.37-6.67, easily spread with spreading ability 2,081.21-2,835.43 mm2, has viscosity 125,000-18,0000 cps and stable 3 mo in a room and 60 °C temperature storage. Conclusion: Momordica charantia leaves can be made become a good cream for skincare cosmetics that more beneficial and profitable than as waste. This skincare creams using vanishing base cream, cold cream, and hand and body cream as base creams.