Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.

2016 ◽  
Vol 128 (3) ◽  
pp. 589-605 ◽  
Author(s):  
Oliver Tusevski ◽  
Branka Vinterhalter ◽  
Dijana Krstić Milošević ◽  
Marina Soković ◽  
Ana Ćirić ◽  
...  
Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 781
Author(s):  
Jaturong Kumla ◽  
Nakarin Suwannarach ◽  
Keerati Tanruean ◽  
Saisamorn Lumyong

Tropical black bolete, Phlebopus portentosus, provides various nutritional benefits and natural antioxidants to humans. In this study, the chemical composition, phenolic compounds, and antioxidant and antimicrobial activities of fresh mushroom samples and samples stored for a period of one year using different preservation methods (drying, brining, and frozen) were investigated. The results indicated that the brining method significantly reduced the protein and fat contents of the mushrooms. The polyphenol and flavonoid contents of the frozen sample were not significantly different from that of the fresh sample. The results revealed that an inhibition value of 50% (IC50) for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay of the extract of the dried and frozen samples was not statistically different from that of the fresh sample. The IC50 value of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) value in the extract of the frozen sample were not found to be significantly different from those of the fresh sample. Furthermore, the lowest degree of antioxidant activity was found in the extract of the brined sample. Additionally, the antimicrobial activities of the extracts of the fresh and frozen samples were not significantly different and both extracts could have inhibited the growth of all tested Gram-positive bacteria and Pseudomonas aeruginosa.


RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41430-41442
Author(s):  
Momna Rubab ◽  
Ramachandran Chelliah ◽  
Kandasamy Saravanakumar ◽  
Jong-Rae Kim ◽  
Daesang Yoo ◽  
...  

Brassica vegetables are well-characterized, containing a wide-spectrum of phenolic compounds that are responsible for their diverse biological activities like antioxidant and antimicrobial activities.


2020 ◽  
Vol 136 ◽  
pp. 111050 ◽  
Author(s):  
Jian Li ◽  
Shi-Ying Huang ◽  
Qianying Deng ◽  
Guiling Li ◽  
Guocheng Su ◽  
...  

2016 ◽  
Vol 41 (3) ◽  
pp. e12345 ◽  
Author(s):  
Mesut Ertan Güneş ◽  
Saliha Şahin ◽  
Cevdet Demir ◽  
Ebru Borum ◽  
Aycan Tosunoğlu

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Vanessa Silva ◽  
Rupesh Kumar Singh ◽  
Nelson Gomes ◽  
Bruno Gonçalves Soares ◽  
Adriana Silva ◽  
...  

Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants.


2010 ◽  
Vol 65 (11-12) ◽  
pp. 701-712 ◽  
Author(s):  
Hedviga Komarovská ◽  
Ján Košuth ◽  
Annalisa Giovannini ◽  
Andrija Smelcerovic ◽  
Sebastian Zuehlke ◽  
...  

The extent of phenotypic variation of St. John’s wort (Hypericum perforatum L.) plants transformed with wild agropine ATCC 15834 Agrobacterium rhizogenes plasmid was evaluated with respect to the number of rol genes integrations. The transfer of TL-DNA to plant explants during each transformation event was incomplete with different rolA, rolB, and rolC copy numbers. Along with typical features representing the hairy root syndrome, an altered size, number and density of dark and translucent glands, changes in ability to synthesize secondary metabolites, and reduced fertility were observed. The highest copy number of transferred rol genes resulted in weak expression of transgenic character and comparable quantitative parameters with the controls. Only 1 out of 11 transgenic clones was able to produce seed progeny and not more than 4 out of its 35 offsprings were positive for rolC gene integration. Sterility of the clones was due to retarded development of both gametophytes


2021 ◽  
Vol 10 (9) ◽  
pp. e8810917942
Author(s):  
Iria Pedroso da Cunha ◽  
Ericson Kubrusly Gonçalves ◽  
Luís Eduardo Silva Nascimento ◽  
Denise Wibelinger de Melo ◽  
Fabíola Carina Biluca ◽  
...  

Araucaria angustifolia var. angustifolia and Araucaria angustifolia var. indehiscens, seeds (common pinhão and monkey pinhão) are consumed after cooking, coats and water represent waste. In this work, pinhão was submitted to different cooking conditions, the water extracts were analyzed to determine and identify their phenolic compounds content, antioxidant activity and antimicrobial potential. Cooking for 45 minutes without addition of sodium chloride resulted in residual water with highest content of phenolic compounds and antioxidant activity (DPPH and FRAP), both for the common pinhão and for the monkey pinhão. Protocatecuic acid, vanillin and coniferaldehyde were identified and quantified by HPLC-ESI-MS/MS as the most prevalent phenolic compounds. No antimicrobial potential was observed against Salmonella enterica Typhimurium (ATCC 14028), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Bacillus cereus (ATCC 11778).


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