scholarly journals Extracellular polysaccharides from Ascomycota and Basidiomycota: production conditions, biochemical characteristics, and biological properties

2015 ◽  
Vol 31 (12) ◽  
pp. 1823-1844 ◽  
Author(s):  
Monika Osińska-Jaroszuk ◽  
Anna Jarosz-Wilkołazka ◽  
Jolanta Jaroszuk-Ściseł ◽  
Katarzyna Szałapata ◽  
Artur Nowak ◽  
...  
2019 ◽  
pp. 58-62
Author(s):  
V. S. Rusaleyev ◽  
О. V. Pruntova ◽  
D. A. Vasilyev

A decrease in therapeutic effect of some live lacto- and bifdobacteria-based drugs for veterinary use has been observed for the last 20 years that urges scientists to search for new microorganisms possessing probiotic properties. Many studies in this feld are focused onBacillus subtilisthat is widespread in the environment and non-pathogenic for animals and humans. Results of tests ofBacillus subtilisfor its biological properties and antagonistic activity aimed at optimization of methodical approaches for detection of strain with the highest antagonistic effect on some opportunistic microorganisms and their further use as probiotics are described. Cultural morphological and biochemical characteristics of the tested strains conformed to the species characteristics ofBacillus subtilis.Tested strains were nonpathogenic for white mice. Tests showed that spore biomass could be prepared both in liquid and on solid nutrient media. Methodically, spore biomass preparation in liquid nutrient medium is preferable. The tests showed that spores emerged from anabiosis non-uniformly and it depended on original seed spore storage period. Spore cultures stored less than one year emerged from anabiosis more quickly. It was found that the spores formed more readily when the cultures were aerated with oxygen as well as that lag-phase culture medium had a stimulating effect onBacillus subtilisspore germination.Bacillus subtilisstrains were found to have antagonistic effect onEscherichia coli, SalmonellaandStaphylococcus. Area of growth inhibition of the said bacteria was 15–20 mm. TestedBacillus subtilisstrains could be proposed for use as probiotics.


1970 ◽  
Vol 16 (8) ◽  
pp. 661-665 ◽  
Author(s):  
T. J. Trust

A Sarcina sp. has been isolated which is antagonistic to the growth of a number of Gram-positive bacteria when grown on solid media. The morphological and biochemical characteristics of the organism are described. The biological properties of the inhibitor have been examined. The data suggest that the inhibitor may interfere with cell wall synthesis.


2012 ◽  
Vol 15 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Florinda Fratianni ◽  
Alfonso De Giulio ◽  
Alfonso Sada ◽  
Filomena Nazzaro

Author(s):  
Я. П. Цвей ◽  
М. В. Тищенко ◽  
С. В. Філоненко

За результатами проведеного моніторингу забур’яненості посівів пшениці озимої, цукрових буряків і ячменю з підсівом багаторічних трав у ланці зернобурякової сівозміни у виробничих умовах встановлено видовий і кількісний склад бур’янів у посівах вищевказаних культур. Все це дає можливість спланувати і застосувати ефективні способи й методи боротьби із сегетальною рослинністю. В результаті проведених досліджень також встановлено, що на видовий склад поширених у посівах сільськогосподарських культур бур’янів має суттєвий вплив поєднання таких чинників, як спосіб основного обробітку ґрунту, попередник і передпопередник, система удобрення та особливості погодних умов веґетаційного періоду, а також біологічні властивості культури, що вирощується. Based on the results of the monitoring of the weediness of winter wheat, sugar beet and barley crops with the sowing of perennial grasses in the link of grain-beet crop rotation, the species and quantity composition of the weeds in the crops of the above-mentioned crops is determined under production conditions. All this makes it possible to plan and apply effective ways and methods of combating segetal vegetation. As a result of the conducted studies it was established that the combination of such factors as the method of basic tillage, the precursor and predecessor, the fertilizer system and the peculiarities of the weather conditions of the growing season, and the biological properties of the cultivated crop, have a significant influence on the species composition of weeds that are widespread in crops.


2020 ◽  
Vol 29 (1) ◽  
pp. 194-199
Author(s):  
Tsenguun Enkhtur ◽  
Tsolmongerel Erdenebayar ◽  
Bujidmaa Baasandorj ◽  
Tuul Nyambal ◽  
Khorloo Yundendorj

Hexachlorocyclohexane (HCH) has been widely used in agriculture of our country to protect crops, vegetable fields, forest soils and plants from pests, and to treat scabies and other diseases in livestock. This pesticide is a highly toxic, carcinogenic, and banned substance that accumulates in human cells, tissues, and the environment over a long period of time. Incubated at 25°C for 3 days. The morphological, physiological, and biochemical characteristics of the two strains that differed in their biological properties and were dominant in the soil sample were determined. To determine the biochemical properties of these cultures, HCHDB1 (collection №1) culture was identified as 100% Bacillus circulans and HCHDB2 (collection № 2) culture was identified as 99% Brevibacillus brevis. Органик нэгдлээр бохирдсон хөрсийг микробиологийн аргаар цэвэршүүлэх боломж Гексахлорциклогексаныг манай улс хөдөө аж ахуйн салбарт өргөнөөр ашиглаж үр тариа, хүнсний ногооны талбай, ойн аж ахуйн хөрс, ургамлыг хортон шавжаас хамгаалах, мал амьтныг хамуу болон бусад төрлийн өвчнийг эмчлэхэд ашиглаж байсан. Энэхүү пестицид нь хүний эс, эд болон байгаль орчинд удаан хугацаагаар хуримтлагддаг, онцгой хортой, хорт хавдар үүсгэх нөлөөтэй, хэрэглэхийг хориглосон химийн бодис юм. Уг судалгаагаар гексахлорциклогексанаар бохирдсон хөрсний дээжинд илэрсэн бактерийн 12 омогийг 1%-ийн гексахлорциклогексан агуулсан хатуу (Nutrient agar) тэжээлт орчинд 25 хэмд 3 өдрийн турш өсгөвөрлөж ялган авсан. Биологийн шинж чанараараа ялгаатай бөгөөд уг хөрсний дээжинд доминант илэрсэн 2 омогийн морфологи, физиологи, биохимийн шинж чанарыг тодорхойлсон. Уг өсгөвөрүүдийн биохимийн шинж чанарыг тодорхойлоход HCHDB1 (коллекцийн №1) өсгөвөр 100%-иар Bacillus circulans, HCHDB2 (коллекцийн №2) өсгөвөр 99%-иар Brevibacillus brevis болохыг тус тус тодорхойлов.  Түлхүүр үг: гексахлорциклогексан, морфологи, физиологи, биохими, тэжээлт орчин


Author(s):  
David A. Agard ◽  
Yasushi Hiraoka ◽  
John W. Sedat

In an effort to understand the complex relationship between structure and biological function within the nucleus, we have embarked on a program to examine the three-dimensional structure and organization of Drosophila melanogaster embryonic chromosomes. Our overall goal is to determine how DNA and proteins are organized into complex and highly dynamic structures (chromosomes) and how these chromosomes are arranged in three dimensional space within the cell nucleus. Futher, we hope to be able to correlate structual data with such fundamental biological properties as stage in the mitotic cell cycle, developmental state and transcription at specific gene loci.Towards this end, we have been developing methodologies for the three-dimensional analysis of non-crystalline biological specimens using optical and electron microscopy. We feel that the combination of these two complementary techniques allows an unprecedented look at the structural organization of cellular components ranging in size from 100A to 100 microns.


Author(s):  
Yih-Tai Chen ◽  
Ursula Euteneuer ◽  
Ken B. Johnson ◽  
Michael P. Koonce ◽  
Manfred Schliwa

The application of video techniques to light microscopy and the development of motility assays in reactivated or reconstituted model systems rapidly advanced our understanding of the mechanism of organelle transport and microtubule dynamics in living cells. Two microtubule-based motors have been identified that are good candidates for motors that drive organelle transport: kinesin, a plus end-directed motor, and cytoplasmic dynein, which is minus end-directed. However, the evidence that they do in fact function as organelle motors is still indirect.We are studying microtubule-dependent transport and dynamics in the giant amoeba, Reticulomyxa. This cell extends filamentous strands backed by an extensive array of microtubules along which organelles move bidirectionally at up to 20 μm/sec (Fig. 1). Following removal of the plasma membrane with a mild detergent, organelle transport can be reactivated by the addition of ATP (1). The physiological, pharmacological and biochemical characteristics show the motor to be a cytoplasmic form of dynein (2).


2015 ◽  
Vol 57 ◽  
pp. 177-187 ◽  
Author(s):  
Jennifer N. Byrum ◽  
William Rodgers

Since the inception of the fluid mosaic model, cell membranes have come to be recognized as heterogeneous structures composed of discrete protein and lipid domains of various dimensions and biological functions. The structural and biological properties of membrane domains are represented by CDM (cholesterol-dependent membrane) domains, frequently referred to as membrane ‘rafts’. Biological functions attributed to CDMs include signal transduction. In T-cells, CDMs function in the regulation of the Src family kinase Lck (p56lck) by sequestering Lck from its activator CD45. Despite evidence of discrete CDM domains with specific functions, the mechanism by which they form and are maintained within a fluid and dynamic lipid bilayer is not completely understood. In the present chapter, we discuss recent advances showing that the actomyosin cytoskeleton has an integral role in the formation of CDM domains. Using Lck as a model, we also discuss recent findings regarding cytoskeleton-dependent CDM domain functions in protein regulation.


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