scholarly journals CULTURAL MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF BACILLUS SUBTILIS STRAINS

2019 ◽  
pp. 58-62
Author(s):  
V. S. Rusaleyev ◽  
О. V. Pruntova ◽  
D. A. Vasilyev
Keyword(s):  
Bacillus Subtilis ◽  
Storage Period ◽  
Biological Properties ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Lag Phase ◽  
Probiotic Properties ◽  
Biochemical Characteristics ◽  
Species Characteristics ◽  
One Year

A decrease in therapeutic effect of some live lacto- and bifdobacteria-based drugs for veterinary use has been observed for the last 20 years that urges scientists to search for new microorganisms possessing probiotic properties. Many studies in this feld are focused onBacillus subtilisthat is widespread in the environment and non-pathogenic for animals and humans. Results of tests ofBacillus subtilisfor its biological properties and antagonistic activity aimed at optimization of methodical approaches for detection of strain with the highest antagonistic effect on some opportunistic microorganisms and their further use as probiotics are described. Cultural morphological and biochemical characteristics of the tested strains conformed to the species characteristics ofBacillus subtilis.Tested strains were nonpathogenic for white mice. Tests showed that spore biomass could be prepared both in liquid and on solid nutrient media. Methodically, spore biomass preparation in liquid nutrient medium is preferable. The tests showed that spores emerged from anabiosis non-uniformly and it depended on original seed spore storage period. Spore cultures stored less than one year emerged from anabiosis more quickly. It was found that the spores formed more readily when the cultures were aerated with oxygen as well as that lag-phase culture medium had a stimulating effect onBacillus subtilisspore germination.Bacillus subtilisstrains were found to have antagonistic effect onEscherichia coli, SalmonellaandStaphylococcus. Area of growth inhibition of the said bacteria was 15–20 mm. TestedBacillus subtilisstrains could be proposed for use as probiotics.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

scholarly journals Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 322 ◽  
Author(s):  
Shuwu Zhang ◽  
Qi Zheng ◽  
Bingliang Xu ◽  
Jia Liu
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Fungal Pathogens ◽  
Biological Control Agent ◽  
Storage Period ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Postharvest Disease ◽  
Economic Losses ◽  
Control Mechanisms

Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

scholarly journals Influence of Dehydrated Wheat/Rice Cereal Matrices on Probiotic Activity of Bifidobacterium animalis ssp. lactis BB-12®

2019 ◽  
Vol 57 (2) ◽  
pp. 147-158 ◽  
Author(s):  
Andreja Leboš Pavunc ◽  
Jasna Novak ◽  
Jagoda Šušković ◽  
Blaženka Kos ◽  
Ksenija Durgo ◽  
...  
Keyword(s):  
Genome Stability ◽  
Storage Period ◽  
Probiotic Strain ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Intestinal Epithelial ◽  
The Novel ◽  
Bifidobacterium Animalis ◽  
Food Matrices

Three novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12® (BB-12®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12®.

scholarly journals Chemical and biological properties of mycobactins isolated from various mycobacteria

1969 ◽  
Vol 115 (5) ◽  
pp. 1031-1045 ◽  
Author(s):  
G. A. Snow ◽  
A. J. White
Keyword(s):  
Growth Promotion ◽  
Biological Properties ◽  
Antagonistic Effect ◽  
Side Chain ◽  
Small Scale ◽  
Lag Phase ◽  
Acid Moiety ◽  
Different Strains ◽  
Mycobacterium Aurum ◽  
Taxonomic Groups

Nine different strains of mycobacteria grown on media deficient in iron all produced mycobactins. Most strains produced one mycobactin in great preponderance. Mycobacteria from clearly distinct taxonomic groups gave mycobactins differing in the structure of their nuclei. One group of taxonomically related mycobacteria produced mycobactins having the same nucleus but with different distributions of side chains within the homologous mixtures. Simple methods are described for identifying mycobactins on a small scale; these may be of value in classifying mycobacteria. Structures are proposed for mycobactin A from Mycobacterium aurum, mycobactin R from M. terrae, mycobactin F, produced together with mycobactin H by M. fortuitum, and mycobactins M and N from M. marinum. The first three of these differ from known mycobactins in details of substitution and configuration of asymmetric centres in the nucleus. Mycobactins M and N are substantially different, having only small acyl groups (acetyl and propionyl respectively) at the hydroxamic acid centre of the mycobactic acid moiety. Both are homologous mixtures having long-chain saturated 3-hydroxy-2-methyl acid fragments in the cobactin moiety. All mycobactins so far isolated promote almost maximal growth of M. johnei at 30ng./ml. in liquid medium. The activity of some mycobactins extends to much lower concentrations, mycobactin S showing significant growth promotion at 0·3ng./ml. Mycobactin M or N in combination with mycobactins having a long side chain in the mycobactic acid moiety exerts a mutually antagonistic effect on the growth of M. johnei, the mixture giving less growth than either mycobactin separately. Mycobactin M also decreases the growth of M. kansasii and M. tuberculosis on liquid media. These antagonistic effects are probably caused by a lengthening of the lag phase.

scholarly journals ANTAGONISTIC ACTIVITY OF ACTINOMYCETES ISOLATED FROM SALINE SOILS NEAR WITH KUMISI LAKE (GEORGIA)

2021 ◽  
pp. 55-59
Author(s):  
M. D. Tsulukidze ◽  
Z. Sh. Lomtatidze ◽  
Q. H. Mamulashvili
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Xanthomonas Campestris ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Pectobacterium Carotovorum ◽  
Rhizobium Radiobacter ◽  
Antibiotic Compounds ◽  
Escherichia Coli B

Aim. To study the antagonistic activity of actinomycetes isolated from soils adjacent to Kumisi Lake (Georgia). Methods. It was used 12 samples of actinomycetes isolated from the soils adjacent to Kumisi Lake. The antibiotic activity of actinomycetes was determined using test strains: Escherichia coli К-12 (B-3254), Bacillus subtilis 26 D, Xanthomonas campestris В-1459, Rhizobium radiobacter C58, Pectobacterium carotovorum EC1by the method of agar blocks. Results. It was revealed that actinomycete samples isolated from soils adjacent to Lake Kumisi shown the most significant inhibitory effect on test strains: Escherichia coli B-3254, Rhizobium radiobacter C58 and Xanthomonas campestris В-1459. The most resistant test-strains, to the studied actinomycetes, were Bacillus subtilis 26 D and Pectobacterium carotovorum EC1. Conclusion. Isolated strain 301 of actinomycetes shown a significant antagonistic effect on all test strains and could be potential for further studies spectrum and properties of antibiotic compounds.

Antagonistic effect ofLactobacillusstrains againstEscherichia coliandListeria monocytogenesin milk

2010 ◽  
Vol 78 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Catalina Aguilar ◽  
Consuelo Vanegas ◽  
Bernadette Klotz
Keyword(s):  
Kinetic Parameters ◽  
Pathogenic Bacteria ◽  
Bacterial Culture ◽  
Growth Curves ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Lag Phase ◽  
Bacterial Populations ◽  
Food Borne ◽  
Mechanism Of Growth

The current work studied four types of binary antagonist/pathogen bacterial culture system, in order to determine the effect of interaction between two strains ofLactobacillus plantarumand two food-borne pathogens,Listeria monocytogenesandEscherichia coli, in whole UHT milk at 37°C. To determine the type of interaction between the two bacterial populations in co-cultures and to evaluate the antagonistic activity of the lactic acid bacteria (LAB) on the pathogenic bacteria, the growth curves, the kinetic parameters, and the pH profiles of mono- and co-cultures were compared. TheLb. plantarumstrains showed different bacteriocin-like inhibitory substance (BLIS) production, auto- and co-inducible. The antibacterial effect of neutralized supernatants of mono and co-cultures harvested at different times of incubation was assessed in order to establish the presence of bacteriocin-like inhibitory-substances (BLIS) and their possible relation to the growth inhibition of the pathogen. The LAB reduced the growth ofEsch. coliand ofList. monocytogenesby 4 and ∼5 log cycles, respectively and influenced other growth kinetic parameters, such as μmaxand lag phase, in the different binary combinations. The growth of the LAB was not relevantly altered by simultaneous growth with the pathogenic strains showing an interaction of amensalism. The pattern of inhibition exerted by the LAB on the pathogens was different;Lb. plantarumLB279 inhibited the growth ofList. monocytogenesmore effectively than that ofEsch. coli. The behaviour ofEsch. coliin co-culture withLb. plantarumWS4174 suggested the presence of metabolic crowding in the mechanism of growth suppression. This exploratory study showed the complexity and specific particularities of the inhibition phenomena between bacterial communities.

scholarly journals The possibility of using bacteria Bacillus in the production of disinfectants

2020 ◽  
Vol 99 (5) ◽  
pp. 436-442
Author(s):  
Anastasia V. Ilyakova ◽  
N. V. Shestopalov ◽  
L. S. Fedorova ◽  
A. S. Belova
Keyword(s):  
Bacillus Subtilis ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Seeding Rate ◽  
Medical Institutions ◽  
Third Stage ◽  
Causative Agents ◽  
Bacillus Spores ◽  
Object Of Study ◽  
Healthcare Associated

Introduction. In recent years, healthcare-associated infections (HAIs) cause ever greater economic and social damage. Microorganisms circulating in medical institutions the causative agents of HAIs, become resistant to most antibiotics and many disinfectants. There is a need to create alternative mechanisms for controlling microorganisms. The purpose of the work is to determine the antagonistic activity of bacteria strains of the genus Bacillus in relation to test microorganisms, as a potential basis for the development of domestic detergents and disinfectants based on probiotics. Material and methods. The strains of Bacillus subtilis 2/10 VKPM B-2896; Bacillus subtilis VKPM B-1283; Bacillus licheniformis VKPM B-5397; Bacillus amyloliquefaciens VKPM B-5462 werethe main object of study. At the first stage of the studies, the antagonistic activity of the strains was studied by the well method, at the second stage of the studies, the antagonistic activity was studied at the test object, and at the third stage of the studies, the effectiveness of the composition containing B. subtilis VKPM B-1283, B.licheniformis VKPM B-5397 and sulfonol strains was studied. Results. As a result of studies, the antagonistic effect of bacilli against test microorganisms was revealed. When surfaces are contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C. albicans ATCC 10231) and subsequent application of Bacillus spores, a significant reduction in the seeding rate with test microorganisms is observed after 24 hours by 97.07-100%. The use of B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 spores together with a detergent component for treating surfaces contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C.albicans ATCC 10231) provides a reduction in microbial seeding after 4 hours by 99.97 %. Conclusions. B.subtilis 2/10 VKPM B-2896, B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 are promising for the use as probiotics, as the basis of detergents and disinfectants.

The possibility of using bacteria Bacillus in the production of disinfectants

2020 ◽  
Vol 99 (5) ◽  
pp. 436-442
Author(s):  
Anastasia V. Ilyakova ◽  
N. V. Shestopalov ◽  
L. S. Fedorova ◽  
A. S. Belova
Keyword(s):  
Bacillus Subtilis ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Seeding Rate ◽  
Medical Institutions ◽  
Third Stage ◽  
Causative Agents ◽  
Bacillus Spores ◽  
Object Of Study ◽  
Healthcare Associated

Introduction. In recent years, healthcare-associated infections (HAIs) cause ever greater economic and social damage. Microorganisms circulating in medical institutions the causative agents of HAIs, become resistant to most antibiotics and many disinfectants. There is a need to create alternative mechanisms for controlling microorganisms. The purpose of the work is to determine the antagonistic activity of bacteria strains of the genus Bacillus in relation to test microorganisms, as a potential basis for the development of domestic detergents and disinfectants based on probiotics. Material and methods. The strains of Bacillus subtilis 2/10 VKPM B-2896; Bacillus subtilis VKPM B-1283; Bacillus licheniformis VKPM B-5397; Bacillus amyloliquefaciens VKPM B-5462 werethe main object of study. At the first stage of the studies, the antagonistic activity of the strains was studied by the well method, at the second stage of the studies, the antagonistic activity was studied at the test object, and at the third stage of the studies, the effectiveness of the composition containing B. subtilis VKPM B-1283, B.licheniformis VKPM B-5397 and sulfonol strains was studied. Results. As a result of studies, the antagonistic effect of bacilli against test microorganisms was revealed. When surfaces are contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C. albicans ATCC 10231) and subsequent application of Bacillus spores, a significant reduction in the seeding rate with test microorganisms is observed after 24 hours by 97.07-100%. The use of B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 spores together with a detergent component for treating surfaces contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C.albicans ATCC 10231) provides a reduction in microbial seeding after 4 hours by 99.97 %. Conclusions. B.subtilis 2/10 VKPM B-2896, B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 are promising for the use as probiotics, as the basis of detergents and disinfectants.

THE BIOLOGICAL PROPERTIES OF 3,6-EPIDITHIADIKETOPIPERAZINES: INHIBITION OF GROWTH OF BACILLUS SUBTILIS BY GLIOTOXINS, SPORIDESMINS, AND CHETOMIN

1966 ◽  
Vol 12 (6) ◽  
pp. 1187-1195 ◽  
Author(s):  
D. Brewer ◽  
D. E. Hannah ◽  
A. Taylor
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Degradation Products ◽  
Disulfide Bridge ◽  
Biological Properties ◽  
Lag Phase ◽  
Fungal Metabolites ◽  
Inhibition Of Growth

The ability of chetomin and the sporidesmin and gliotoxin groups of fungal metabolites to inhibit the growth of Bacillus subtilis (HLX 373) has been examined. With the exception of gliotoxin dibenzoate, compounds having the 3,6-epidithiadiketopiperazine structure, when added to the culture medium before inoculation, increased the lag phase of growth; this increase was influenced by the number of organisms in the inoculum and the concentration of the metabolite. When the disulfide bridge was eliminated chemically, the resulting degradation products were biologically inactive.

scholarly journals THE EFFECT OF INTERNAL ENVIRONMENT OF ANIMALS ON PROPERTIES OF PROBIOTIC BACTERIA

2014 ◽  
Vol 19 ◽  
pp. 89-94
Author(s):  
V. O. Aheyev
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biological Properties ◽  
Antagonistic Activity ◽  
Probiotic Bacteria ◽  
Internal Environment ◽  
Size And Shape

The paper depicts the results of the study of influence of internal environment of animals on preservation of initial biological properties of probiotic bacteria. The increased by 10–15 % antagonistic activity was observed in Bacillus subtilis 44-p strain after its translocation to the laboratory rabbits while for B. subtilis B3 the changes in the morphology of colonies (size and shape) were noticed. Nevertheless, the properties of the studied lactic acid bacteria after exposition to the internal environment of the macroorganism remain unchanged.

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