Stability properties of natural estrogen conjugates in different aqueous samples at room temperature and tips for sample storage

2021 ◽  
Author(s):  
Jun Zhang ◽  
Yi-ping Wan ◽  
Ze-hua Liu ◽  
Hao Wang ◽  
Zhi Dang ◽  
...  
Keyword(s):  
Room Temperature ◽  
Aqueous Samples ◽  
Sample Storage ◽  
Stability Properties

scholarly journals The effect of storage conditions on samples for the evaluation of copper status in blesbok (Damaliscus pygargus phillipsi)

2002 ◽  
Vol 73 (3) ◽  
Author(s):  
M. Quan ◽  
M.S. Mulders ◽  
D.G.A. Meltzer
Keyword(s):  
Superoxide Dismutase ◽  
Liquid Nitrogen ◽  
Liver Tissue ◽  
Room Temperature ◽  
Storage Conditions ◽  
Superoxide Dismutase Activity ◽  
Activity Levels ◽  
Sample Storage ◽  
Live Animal ◽  
Jersey Cows

Investigaltions to determine the effect of sample storage on the concentration of copper in liver tissue and on the activity of erythrocyte superoxide dismutase were undertaken in preparation for a study of blesbok (Damaliscus pygargus phillipsi) that were suspected to be suffering from copper deficiency. Two liver samples were collected from each of 20 culled blesbok in a manner that simulated the collection of biopsies from the live animal. These samples were stored either in 10 % formalin or frozen at -20 °C until analysed 4 1/2 months later. The effect of different methods of sample storage on superoxide dismutase activity was determined. Erythrocytes collected from 3 Jersey cows and 5 culled blesbok were washed and divided into 0.5m portions, stored at room temperature (~20 °C), in a refrigerator (4 °C), frozen at -20 °C in a freezer, and in liquid nitrogen (-200 °C). An analysis of superoxide dismutase activity was undertaken using a commercial assay kit at intervals of 2-4 days until the levels of activity had fallen significantly. The copper concentration in formalin-preserved liver samples was significantly lower than that measured in frozen liver tissue apparently as a result of leaching. The activity of superoxide dismutase in cattle blood was unchanged for 4 days at room temperature but fell appreciably after 2 days at 4 °C and -20 °C. Enzyme activity remained unchanged for 200 days in erythrocytes stored in liquid nitrogen. Superoxide dismutase activity levels in healthy blesbok were considerably lower than those measured in Jersey cows and remained unaffected for up to 6 days in samples stored at 4 °C and 20 °C. The level of activity fell significantly thereafter. Samples stored in liquid nitrogen were unchanged after 40 days.

scholarly journals Long-term room temperature preservation of corpse soft tissue: an approach for tissue sample storage

2011 ◽  
Vol 2 (1) ◽  
pp. 17 ◽  
Author(s):  
Mariela Caputo ◽  
Luis A Bosio ◽  
Daniel Corach
Keyword(s):  
Soft Tissue ◽  
Room Temperature ◽  
Tissue Sample ◽  
Sample Storage

Metastable Superstructures in RuSr2Gd1.4Ce0.6Cu2O10-δ?Superconductor Based on TEM Observation at Room Temperature

2001 ◽  
Vol 689 ◽  
Author(s):  
Li Yang ◽  
J. M. Vieira ◽  
Kaibin Tang ◽  
Guien Zhou
Keyword(s):  
Electron Beam ◽  
Electron Diffraction ◽  
Room Temperature ◽  
Storage Time ◽  
Point Of View ◽  
Tetragonal Symmetry ◽  
Sample Storage ◽  
Unconventional Superconductor ◽  
Fluorite Type ◽  
Structural Point

ABSTRACTThree types of metastable modulations in Ru-based superconductor RuSr2Gd1.4Ce0.6Cu2O10-δ are observed by electron diffraction at room temperature and reported in this paper. The modulations are sensitive to the irradiation of the electron beam and the sample storage time. Having the tetragonal symmetry (I4/mmm) with a=0.384nm, c=2.864nm, the structure of RuSr2Gd1.4Ce0.6Cu2O10-δ?resembles that of YBa2Cu3O7-δ by inserting a fluorite type Gd1.4Ce0.6O2 layer instead of the Y layer and Ru ions residing in the Cu(1) site. In this compound, superconductivity is confined to the CuO2 layer while magnetism stems from the RuO2 layer. The metastable modulations display the interaction between the two layers from the structural point of view. The results suggest that although, superconductivity and magnetism are decoupled from each other in the unconventional superconductor, the effect may be limited by metastable lattice modulation.

Stable Plasma Sample Storage in Acetonitrile for Angiotensin and Aldosterone Analysis

2020 ◽  
Author(s):  
Xuefei Wei ◽  
Yanyang Wang ◽  
Wenbo Zhu ◽  
Jingjing Li ◽  
Lu Peng ◽  
...  
Keyword(s):  
Room Temperature ◽  
Plasma Sample ◽  
Sample Storage ◽  
Angiotensin I ◽  
Storage Method ◽  
Collaborative Studies ◽  
Stable Plasma

Abstract Background Angiotensin I, II (AI, AII) and aldosterone are unstable in plasma specimens at room temperature, making it difficult for collect samples for remote regions in centralized and collaborative studies. Here we introduce a stable storage method which do not require cold conditions.. Methods Acetonitrile was added to the plasma to 60%, and then the supernatants were kept at 4°C and room temperature for 0, 1, 2, 3, 10 and 30 days. AI, AII and aldosterone were extracted and analyzed by chemiluminescence immunoassays. Results AI, AII and aldosterone were well retained in the supernatant under this method. The intra- and inter-day CVs of this method were all below 10%. The levels of AI, AII and aldosterone by this method remained stable for 30 days at room temperature. Conclusion Addition of 60% acetonitrile in the plasma provides a stable storage method for clinical AI, AII and aldosterone.

The Technology and Storage Stability Studies of Yam Functional Food

2014 ◽  
Vol 687-691 ◽  
pp. 4515-4519
Author(s):  
Jian Chun Zhao
Keyword(s):  
Functional Food ◽  
Room Temperature ◽  
Storage Time ◽  
Storage Stability ◽  
Stability Studies ◽  
Sample Storage ◽  
Room Temperature Sample ◽  
Index Changes ◽  
And Storage ◽  
Temperature Sample

In this paper, yam functional food technology and storage stability were studied, the results indicate that storage time and temperature on the results significantly higher than room temperature sample storage, cryogenic sample index changes significantly, the moisture content of the sample affect the sensory scores larger.

scholarly journals Systematic Analysis of Impact of Sampling Regions and Storage Methods on Fecal Gut Microbiome and Metabolome Profiles

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Yali Liang ◽  
Tianyu Dong ◽  
Minjian Chen ◽  
Lianping He ◽  
Tingzhang Wang ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Room Temperature ◽  
Storage Conditions ◽  
Minimal Effect ◽  
Sample Storage ◽  
Short Term ◽  
Room Temperature Storage ◽  
Stool Samples ◽  
And Storage ◽  
Temperature Storage

ABSTRACT The contribution of human gastrointestinal (GI) microbiota and metabolites to host health has recently become much clearer. However, many confounding factors can influence the accuracy of gut microbiome and metabolome studies, resulting in inconsistencies in published results. In this study, we systematically investigated the effects of fecal sampling regions and storage and retrieval conditions on gut microbiome and metabolite profiles from three healthy children. Our analysis indicated that compared to homogenized and snap-frozen samples (standard control [SC]), different sampling regions did not affect microbial community alpha diversity, while a total of 22 of 176 identified metabolites varied significantly across different sampling regions. In contrast, storage conditions significantly influenced the microbiome and metabolome. Short-term room temperature storage had a minimal effect on the microbiome and metabolome profiles. Sample storage in RNALater showed a significant level of variation in both microbiome and metabolome profiles, independent of the storage or retrieval conditions. The effect of RNALater on the metabolome was stronger than the effect on the microbiome, and individual variability between study participants outweighed the effect of RNALater on the microbiome. We conclude that homogenizing stool samples was critical for metabolomic analysis but not necessary for microbiome analysis. Short-term room temperature storage had a minimal effect on the microbiome and metabolome profiles and is recommended for short-term fecal sample storage. In addition, our study indicates that the use of RNALater as a storage medium of stool samples for microbial and metabolomic analyses is not recommended. IMPORTANCE The gastrointestinal microbiome and metabolome can provide a new angle to understand the development of health and disease. Stool samples are most frequently used for large-scale cohort studies. Standardized procedures for stool sample handling and storage can be a determining factor for performing microbiome or metabolome studies. In this study, we focused on the effects of stool sampling regions and stool sample storage conditions on variations in the gut microbiome composition and metabolome profile.

scholarly journals Impact of Ambient Temperature Sample Storage on the Equine Fecal Microbiota

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 819
Author(s):  
Michelle Martin de Bustamante ◽  
Caryn Plummer ◽  
Jennifer MacNicol ◽  
Diego Gomez
Keyword(s):  
Ambient Temperature ◽  
Room Temperature ◽  
High Throughput Sequencing ◽  
Fecal Microbiota ◽  
Rrna Gene ◽  
Sample Storage ◽  
Sample Collection ◽  
Microbial Composition ◽  
Fecal Samples ◽  
Community Membership

Sample storage conditions are an important factor in fecal microbiota analyses in general. The objective of this study was to investigate the effect of sample storage at room temperature on the equine fecal microbiota composition. Fecal samples were collected from 11 healthy horses. Each sample was divided into 7 sealed aliquots. One aliquot was immediately frozen at −80 °C; the remaining aliquots were stored at room temperature (21 to 22 °C) with one transferred to the freezer at each of the following time points: 6, 12, 24, 48, 72 and 96 h. The Illumina MiSeq sequencer was used for high-throughput sequencing of the V4 region of the 16S rRNA gene. Fibrobacteraceae (Fibrobacter) and Ruminococcaceae (Ruminococcus) were enriched in samples from 0 h and 6 h, whereas taxa from the families Bacillaceae, Planococcaceae, Enterobacteriaceae and Moraxellaceae were enriched in samples stored at room temperature for 24 h or greater. Samples frozen within the first 12 h after collection shared similar community membership. The community structure was similar for samples collected at 0 h and 6 h, but it was significantly different between samples frozen at 0 h and 12 h or greater. In conclusion, storage of equine fecal samples at ambient temperature for up to 6 h before freezing following sample collection had minimal effect on the microbial composition. Longer-term storage at ambient temperature resulted in alterations in alpha-diversity, community membership and structure and the enrichment of different taxa when compared to fecal samples immediately frozen at −80 °C.

scholarly journals Analysis of SARS-CoV-2 RNA Stability and Infectivity in Oropharyngeal Swab Samples

2021 ◽  
Author(s):  
Markus Neumann ◽  
Marica Grossegesse ◽  
Daniel Bourquain ◽  
Lars Schaade ◽  
Andreas Nitsche
Keyword(s):  
Room Temperature ◽  
Infectious Virus ◽  
Optimal Transport ◽  
Rna Stability ◽  
Genomic Rna ◽  
Sample Storage ◽  
Virus Particles ◽  
Reliable Detection ◽  
Good Sample ◽  
Oropharyngeal Swab

Abstract The reliable detection of SARS-CoV-2 genomic RNA and infectious virus particles from patient samples requires a good sample quality. This is especially critical when the sample has to be transported to the analysing laboratory which can take several days. To determine optimal transport conditions, we simulated oropharyngeal swab samples using defined virus amounts and stored the samples at 4 °C or at room temperature for up to four days. Moreover, we analysed the influence of dry swabs in comparison to swabs stored in transport medium. Our results show that care should be taken when analysing samples for infectious SARS-CoV-2 particles since infectivity is strongly influenced by sample storage.

Effect of duration and temperature of storage on serum analyte stability: examination of 14 selected radioimmunoassay procedures.

1982 ◽  
Vol 28 (1) ◽  
pp. 164-165 ◽  
Author(s):  
N P Kubasik ◽  
M Ricotta ◽  
T Hunter ◽  
H E Sine
Keyword(s):  
Room Temperature ◽  
Clinical Laboratory ◽  
Immunoglobulin E ◽  
Sample Storage ◽  
Stability Examination ◽  
Blood Spot

Abstract We determined appropriate temperatures for sample storage and the resulting stability of 14 analytes commonly radioimmunoassayed in the clinical laboratory. Serum specimens to be tested for concentrations of cholylglycine, cortisol, digoxin, ferritin, follitropin, immunoglobulin E, lutropin, prolactin, thyroxin (also blood-spot thyroxin), triiodothyronine, and triiodothyronine uptake could be stored for up to two weeks at room temperature, refrigerated, or frozen without any loss of analyte activity. Specimens for insulin testing require freezing or refrigeration, and specimens for gastrin testing should be stored at -70 degrees C for optimal results.

scholarly journals Alcohol keeps eDNA at the party longer

2021 ◽  
Vol 4 ◽  
Author(s):  
Sarah Licul ◽  
Rachael Impey ◽  
Andrew Weeks
Keyword(s):  
Room Temperature ◽  
Field Studies ◽  
Dna Degradation ◽  
Storage Conditions ◽  
Sample Storage ◽  
Time Intervals ◽  
Silica Bead ◽  
Scientific Analysis ◽  
Protocol Optimisation ◽  
Water Filters

For a typical eDNA water study, water will be filtered on site, before prompt transfer to a laboratory for DNA extraction and required scientific analysis. In a setting where transport is quick and available, this is a straightforward process. However, many of our studies can occur in remote Australia where sample preservation presents many logistical challenges. Typically, we advise clients to store eDNA water filters after sampling below 4 °C to ensure minimal DNA degradation. For many clients however, field studies often occur in an isolated setting without adequate refrigeration facilities, and as such present challenges for this process. Rather than compromise on sample integrity, EnviroDNA conducted a pilot study into the use of alternate preservation methods on our most commonly used 0.22 mm Sterivex filters. With help from our friendly neighbourhood goldfish tank, our standard 4 °C protocol was compared to a variety of conditions including filled ethanol filters, flushed ethanol filters, lysis buffer and silica bead storage conditions at both 4 °C and room temperature. The study, conducted at various time points over 14-days, used qPCR to quantify the amount of DNA extracted from the filter. Our results revealed that storage within or using flushed ethanol, allowed the samples to be stored for longer time intervals at room temperature, with similar, or in some cases, improved DNA elutions. This protocol optimisation has allowed us to offer an alternate sample storage protocol for clients, expanding the availability and accessibility of eDNA biodiversity assessments around Australia.

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