Gastroprotective activity of the ethanolic extract and hexane phase of Combretum duarteanum Cambess. (Combretaceae)

The acute toxicity, the antioxidant activity, and the pharmacological activity on the gastrointestinal tract of rodents of the ethanolic extract (TFEE) from the bark ofTerminalia fagifoliaMart. & Zucc. (Combretaceae) and of its aqueous (TFAqF), hydroalcoholic (TFHAF), and hexanic (TFHEXF) partition fractions have been evaluated. TFEE presented low acute toxicity, antioxidant, and antiulcerogenic activity against ethanol-induced ulcers, which was partially blocked by pretreatment with L-NAME and indomethacin. It reduced the total acidity and raised the pH of gastric secretion. Additionally, TFEE delayed gastric emptying and slightly inhibited the small intestinal transit and also presented a weakly antidiarrheal activity. The antiulcerogenic and antioxidant activity were also detected in TFAqF and TFHAF but not in TFHEXF. The antisecretory and gastroprotective activity of TFEE partially involve the nitric oxide and prostaglandin participation. Nevertheless, TFEE, TFAqF, and TFHAF drastically reduced the mucus layer adhered to the gastric wall of rats treated with ethanol or indomethacin. Complementary studies are required in order to clarify the paradox of the presence of a gastroprotector activity in this plant that, at the same time, reduces the mucus layer adhered to the gastric wall.

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Gastroprotective Effect of Geopropolis fromMelipona scutellarisIs Dependent on Production of Nitric Oxide and Prostaglandin

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/459846 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 10

Author(s):

Jerônimo Aparecido Ribeiro-Junior ◽

Marcelo Franchin ◽

Miriam Elias Cavallini ◽

Carina Denny ◽

Severino Matias de Alencar ◽

...

Keyword(s):

Nitric Oxide ◽

Ethanolic Extract ◽

Mechanisms Of Action ◽

The Other ◽

Medicine Use ◽

Gastroprotective Activity ◽

Sh Groups ◽

Antisecretory Activity ◽

Ulcerative Lesions ◽

Prior Administration

The aim of this study was to evaluate the gastroprotective activity of ethanolic extract of geopropolis (EEGP) fromMelipona scutellarisand to investigate the possible mechanisms of action. The gastroprotective activity of the EEGP was evaluated using model ulcer induced by ethanol. To elucidate the possible mechanisms of action, we investigated the involvement of the nonprotein sulfhydryl (NP-SH) groups, nitric oxide and prostaglandins. In addition, the antisecretory activity of EEGP was also evaluated by pylorus ligated model. The EEGP orally administrated (300 mg/kg) reduced the ulcerative lesions induced by the ethanol (P<0.05). Regarding the mechanism of action, the prior administration of nitric oxide and prostaglandins antagonists suppressed the activity of gastroprotective EEGP (P<0.05). On the other hand the gastroprotective activity of EEGP was kept in the group pretreated with the antagonist of the NP-SH groups; furthermore the antisecretory activity was not significant (P>0.05). These results support the alternative medicine use of geopropolis as gastroprotective and the activities observed show to be related to nitric oxide and prostaglandins production.

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Gastroprotective activity of hot ethanolic extract Alpinia calcarata rhizome in rats

Ceylon Journal of Medical Science ◽

10.4038/cjms.v48i1.4838 ◽

2005 ◽

Vol 48 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

L. S. R. Arambewela ◽

L. D. A. M. Arawwawala ◽

W. D. Ratnasooriya

Keyword(s):

Ethanolic Extract ◽

Gastroprotective Activity

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Optimization of phenolic compounds extraction from Campomanesia lineatifolia leaves

Rodriguésia ◽

10.1590/2175-7860202071043 ◽

2020 ◽

Vol 71 ◽

Author(s):

Nívea Cristina Vieira Neves ◽

Morgana Pinheiro de Mello ◽

Juliana Mendes Amorim ◽

André Augusto Gomes Faraco ◽

Rachel Oliveira Castilho

Keyword(s):

High Performance ◽

Lower Cost ◽

Ethanolic Extract ◽

Extraction Process ◽

Antiulcer Activity ◽

Gastroprotective Activity ◽

Edible Species ◽

Ethanolic Extraction ◽

Compound Concentration ◽

Tnf Inhibition

Abstract Campomanesia lineatifolia (gabiroba) is a native edible species found in the Amazon Rainforest. Previous studies have demonstrated the flavonoid nature of ethanolic extract from the C. lineatifolia leaves, in addition to gastroprotective activity and TNF inhibition. However, the extraction process used was long and consumed a large amount of solvent. Therefore, the objective of this study was to obtain a bioactive extract rich in phenolics, in an extractive method of simpler, faster, and lower-cost execution. The C. lineatifolia leaves were dried and crushed, and the extractions were carried out in different solvents/mixtures (ethanol, methanol, ethyl acetate, and water) under ultrasonic bath (UB), electromagnetic stirring, and continuous reflux extraction (R). The extraction efficiency was evaluated by the flavonoid major compound concentration in the extracts, in a method developed by ultra-high performance liquid chromatography (UHPLC). Injection and pattern matching tests, and UHPLC analyses coupled to ultraviolet spectrometry were conducted to identify catechin and quercitrin. It has been demonstrated that the ethanolic extraction by R and the mixture of ethanol: water (8:2) by UB represented optimized methods in obtaining the flavonoid compounds identified. Thus, the results may contribute to chemical-biological extract standardization for gastric antiulcer activity evaluation.

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Gastroprotective Activity of Sterculia striata A. St. Hil. & Naudin (Malvaceae) in Rodents

Zeitschrift für Naturforschung C ◽

10.1515/znc-2012-3-408 ◽

2012 ◽

Vol 67 (3-4) ◽

pp. 163-171

Author(s):

Joubert A. Sousa ◽

Irisdalva S. Oliveira ◽

Francilene V. Silva ◽

Danielly A. Costa ◽

Mariana H. Chaves ◽

...

Keyword(s):

Nitric Oxide ◽

Methyl Ester ◽

Gastric Secretion ◽

Catalase Activity ◽

Gastric Lesion ◽

Ischemia Reperfusion ◽

Ethanolic Extract ◽

Antioxidant Compounds ◽

Gastric Lesions ◽

Gastroprotective Activity

The Sterculia striata ethanolic extract (Ss-EtOH) inhibited gastric lesions induced by ethanol, HCl/ethanol, and ischemia/reperfusion, but not those induced by indomethacin, and did not alter the gastric secretion. Ss-EtOH restored the catalase activity and content of nonprotein sulfhydryl groups in the stomach of mice treated with ethanol. The gastroprotection induced by Ss-EtOH in the ethanol-induced gastric lesion model was abolished by NG-nitro- L-arginine methyl ester (L-NAME) pretreatment, suggesting the involvement of nitric oxide and antioxidant compounds, but not prostaglandins, in this activity. Lupeol obtained from Ss-EtOH promoted gastroprotection as well as the extract at the same dose, and it must therefore contribute to the observed effects

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GASTROPROTECTIVE ACTIVITY OF EQUISETUM HYEMALE IN EXPERIMENTAL GASTRIC ULCER RAT MODELS

FARMACIA ◽

10.31925/farmacia.2021.2.22 ◽

2021 ◽

Vol 69 (2) ◽

pp. 356-366

Author(s):

NAGHMANA ◽

MUHAMMAD NAVEED MUSHTAQ

Keyword(s):

Gastric Ulcer ◽

Ethanolic Extract ◽

Control Group ◽

Total Acidity ◽

Gastroprotective Activity ◽

Traditional Use ◽

Rat Models ◽

Stomach Pain ◽

Equisetum Hyemale ◽

Scoring Index

Equisetum hyemale is traditionally used for dyspepsia and stomach pain. The aim of the present study was to evaluate the gastroprotective activity of the aqueous ethanolic extract of the aerial parts of Equisetum hyemale in gastric ulcer rat models. Gastric ulcer models were induced by ethanol (5 mL/kg bw), acetylsalicylic acid (ASA) (200 mg/kg bw) and pylorus ligation separately. The pH, total acidity, ulcer scoring index and histopathological evaluation were performed. Oral administration of Equisetum hyemale extract (250 and 500 mg/kg bw) significantly reduced the development of gastric lesions in all gastric ulcer models. The pH, total acidity and ulcer scoring index also decreased significantly when compared with the Diseased control group. Histopathological studies are in good agreement with the biochemical findings. Equisetum hyemale might show its gastroprotective activity by decreasing oxidative damage, blockage of H2 receptors activation, increase prostaglandins secretion and formation of mucus layer in different gastric ulcer models. The findings of the present study validate the traditional use of Equisetum hyemale to treat stomach pain, however the determination of phytochemical compounds responsible for gastroprotective activity is required for further confirmation.

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Gastroprotective Activity of Parastrephia quadrangularis (Meyen), Cabrera from the Atacama Desert

Molecules ◽

10.3390/molecules23092361 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2361 ◽

Cited By ~ 2

Author(s):

Alejandro Ardiles ◽

Ruth Barrientos ◽

Mario Simirgiotis ◽

Jorge Bórquez ◽

Beatriz Sepúlveda ◽

...

Keyword(s):

Mass Spectrometry ◽

Mouse Model ◽

Atacama Desert ◽

Ethanolic Extract ◽

Biologically Active ◽

Gastroprotective Activity ◽

Control Drug ◽

Orbitrap Mass Spectrometry ◽

Clerodane Diterpenes ◽

First Time

Forty-three metabolites including several methoxylated flavonoids, tremetones, and ent-clerodane diterpenes were accurately identified for the first time in the ethanolic extract of P. quadrangularis by means of hyphenated UHPLC-quadrupole Orbitrap mass spectrometry, and seven isolated compounds were tested regarding gastroprotective activity using the HCl/EtOH-induced lesion model in mice. A new tremetone (compound 6) is reported based on spectroscopic evidence. The isolated clerodanes and tremetones showed gastroprotective activity in a mouse model, evidenced by compound 7 (p-coumaroyloxytremetone), which showed the highest gastroprotective activity (76%), which was higher than the control drug lansoprazole (72%). Our findings revealed that several constituents of this plant have gastroprotective activity, and particularly, p-coumaroyloxytremetone could be considered as a lead molecule to explore new gastroprotective agents. This plant is a rich source of biologically active tremetones and terpenoids which can support the ethnobotanical use of the plant.

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