scholarly journals Optimization of phenolic compounds extraction from Campomanesia lineatifolia leaves

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Nívea Cristina Vieira Neves ◽  
Morgana Pinheiro de Mello ◽  
Juliana Mendes Amorim ◽  
André Augusto Gomes Faraco ◽  
Rachel Oliveira Castilho
Keyword(s):  
High Performance ◽  
Lower Cost ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Antiulcer Activity ◽  
Gastroprotective Activity ◽  
Edible Species ◽  
Ethanolic Extraction ◽  
Compound Concentration ◽  
Tnf Inhibition

Abstract Campomanesia lineatifolia (gabiroba) is a native edible species found in the Amazon Rainforest. Previous studies have demonstrated the flavonoid nature of ethanolic extract from the C. lineatifolia leaves, in addition to gastroprotective activity and TNF inhibition. However, the extraction process used was long and consumed a large amount of solvent. Therefore, the objective of this study was to obtain a bioactive extract rich in phenolics, in an extractive method of simpler, faster, and lower-cost execution. The C. lineatifolia leaves were dried and crushed, and the extractions were carried out in different solvents/mixtures (ethanol, methanol, ethyl acetate, and water) under ultrasonic bath (UB), electromagnetic stirring, and continuous reflux extraction (R). The extraction efficiency was evaluated by the flavonoid major compound concentration in the extracts, in a method developed by ultra-high performance liquid chromatography (UHPLC). Injection and pattern matching tests, and UHPLC analyses coupled to ultraviolet spectrometry were conducted to identify catechin and quercitrin. It has been demonstrated that the ethanolic extraction by R and the mixture of ethanol: water (8:2) by UB represented optimized methods in obtaining the flavonoid compounds identified. Thus, the results may contribute to chemical-biological extract standardization for gastric antiulcer activity evaluation.

scholarly journals Quantification and Optimization of Ethanolic Extract Containing the Bioactive Flavonoids from Millettia pulchra Radix

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3641
Author(s):  
Thanh-Hoa Vo ◽  
Chia-Ching Liaw ◽  
Yu-Chi Lin ◽  
Duc Hanh Nguyen ◽  
Thi Tuyet Nhung Nguyen ◽  
...  
Keyword(s):  
High Performance ◽  
Ethanolic Extract ◽  
Predictive Performance ◽  
Extraction Process ◽  
Ich Guidelines ◽  
Potential Resource ◽  
Ann Models ◽  
Photodiode Array ◽  
Artificial Neural Network Ann ◽  
Optimized Conditions

Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, anemia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pulchra radix, develop and validate the HPLC-PDA method to determine their contents, and optimize its extraction. Four major flavonoid derivatives (karanjin, lanceolatin B, 2”,2”-dimethylpyrano-[5″,6″:7,8]-flavone, and pongamol) were isolated using silica gel column chromatography, crystallization techniques in large amounts with high purities (>95%). A simple, accurate high-performance liquid chromatography–photodiode array (HPLC–PDA) detection method has been developed and validated with significantly statistical impacts according to International Conference on Harmonization (ICH) guidelines. The Response Surface Methodology (RSM), Artificial Neural Network (ANN) models were employed to predictive performance and optimization of the extraction process. The optimized conditions for the extraction of major flavonoids were: extraction time (twice), solvent/material ratio (9.5), and ethanol concentration (72.5%). Our research suggests an effective method, which will be helpful for quality control in the pharmaceutical development of this species.

scholarly journals Optimization of sunflower head pectin extraction by ammonium oxalate and the effect of drying conditions on properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuemei Ma ◽  
Jiayi Yu ◽  
Jing Jing ◽  
Qian Zhao ◽  
Liyong Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Ammonium Oxalate ◽  
Extraction Process ◽  
Size Exclusion ◽  
Pharmaceutical Industries

AbstractPectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid–solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.

scholarly journals Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Sarah Raquel Gomes de Lima-Saraiva ◽  
Fernanda Granja da Silva Oliveira ◽  
Raimundo Gonçalves de Oliveira Junior ◽  
Camila de Souza Araújo ◽  
Ana Paula de Oliveira ◽  
...  
Keyword(s):  
High Performance ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Physicochemical Analysis ◽  
Minimal Bactericidal Concentration ◽  
Native Plant ◽  
Protection Factor ◽  
Dpph Method ◽  
High Concentrations

Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.

scholarly journals EVALUATION OF ANTIPYRETIC AND ANTIULCER ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF TREMA ORIENTALIS L. IN ALBINO WISTAR RATS

2019 ◽  
pp. 149-154
Author(s):  
VANITA G KANASE ◽  
JYOTI SINGH
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Antiulcer Activity ◽  
Brewer’S Yeast ◽  
Brewer's Yeast ◽  
Trema Orientalis ◽  
Percentage Yield ◽  
Ulcer Model ◽  
Dried Extract

Objectives: The purpose of the present study was aimed at evaluating the antipyretic and antiulcer activity on leaves of Trema orientalis L. (family Cannabaceae) on albino Wistar rats. The antipyretic and antiulcer activity of the ethanolic extract of the T. orientalis L. leaves in three different concentrations (100, 200, and 300 mg/kg) was compared with standard paracetamol and pantoprazole, which was evaluated by employing Brewer’s yeast-induced pyrexia and ethanol-induced ulcer model. The biochemical parameters such as the volume of gastric juice secretion, pH, total acidity, ulcer index, percentage protection, and lipid peroxidation were also studied. Methods: T. orientalis leaves were extracted with ethanol by the Soxhlet extraction method. The dried extract was used for further phytochemical and pharmacological analysis. The antipyretic effect was studied using Brewer’s yeast-induced pyrexia. The ethanol-induced ulcer model was used to study the antiulcer effect. Results: The percentage yield of ethanolic extract of T. orientalis leaves was found to be 6.71% w/w, respectively. The extract showed significant antipyretic and antiulcer effect when compared with standard paracetamol and pantoprazole. Conclusion: The ethanolic extract of T. orientalis has significant antipyretic and antiulcer action.

scholarly journals Effect of coffee reduction on constituent concentration in an energy-efficient process of ultrasonic extraction

2015 ◽  
Vol 19 (4) ◽  
pp. 1373-1377
Author(s):  
Cheng-Chi Wang ◽  
Shane-Rong Sheu ◽  
Her-Terng Yau ◽  
Ming-Jyi Jang
Keyword(s):  
Energy Efficient ◽  
High Performance ◽  
Extraction Process ◽  
Manufacturing Cost ◽  
Negative Results ◽  
Ultrasonic Process ◽  
Oxidation Resistant ◽  
Artery Disease ◽  
Save Energy ◽  
The Brain

Coffee is one of the popular beverage; its constituents include caffeine, oxidation resistant aromatic constituents, protein, tannin, and fat. It is indicated in literatures that a proper amount of coffee stimulates the brain and enhances memory, but excessive coffee causes negative results, such as coronary artery disease, high blood pressure, heart disease and kidney disease. This study used high-performance ultrasonic process to discuss the effect of pulverized coffee reduction on the constituent concentration. It further compared the constituent concentrations obtained in different extraction periods. The experimental results show that the coffee aroma constituents can be extracted effectively by ultrasonic process without any organic solvent, and the constituent concentration does not decrease with the addition of pulverized coffee. Therefore, the consumption of pulverized coffee can be reduced greatly by using the proposed. The time of extraction process can be shortened, so as to save energy. The most important point is to reduce the enterprises manufacturing cost and to increase the profit.

scholarly journals Effective Utilization of Pineapple Waste, Evaluation of Ferulic Acid, Micro Nutrients and Antioxidant Property

2020 ◽  
Vol 2 (1) ◽  
pp. 30
Keyword(s):  
Antioxidant Activity ◽  
Ferulic Acid ◽  
High Performance ◽  
Soxhlet Extraction ◽  
Antioxidant Property ◽  
Extraction Process ◽  
Fruit Peel ◽  
Manganese Zinc ◽  
Pineapple Peel ◽  
Phosphorus Iron

India is the second-largest producer of fruit and vegetable producers. Usually, after fruit intake, fruit peel is left as waste. The main bioactive components of pineapple are phenolic compounds, β-carotene, ascorbic acid, and flavonoids. Ferulic acid is a phenolic acid widely used in the nutritional and cosmetic fields. In this study, pineapple peel was dried, powdered, and vitamin content (A, B, B1, B2, B6, B12 & C), calcium, potassium, phosphorus, iron, manganese, zinc and food fiber were analyzed. Moreover, in the present study, traditional and non-conventional processes such as Soxhlet extraction, supreme fluid extraction, and normal solvent extraction was used for the extraction of ferulic acid, which is a precursor for vanillin synthesis. The quantification of ferulic acid was done by High performance fluid chromatographic (HPL C) method. After the above-mentioned extraction process overall phenolic and antioxidant activity were also evaluated and compared. The highest concentrations of ferulic acid (0.7696g/100 g), phenolic compound (2.365mg / GAE), antioxidant activity (45 percent), and yield (90.5 %) were obtained for Soxhlet extraction using methanol.

Gastroprotective activity of the ethanolic extract and hexane phase of Combretum duarteanum Cambess. (Combretaceae)

2012 ◽  
Vol 67 (3) ◽  
pp. 492-502 ◽  
Author(s):  
Gedson Rodrigues de Morais Lima ◽  
Camila de Albuquerque Montenegro ◽  
Heloina de Sousa Falcão ◽  
Neyres Zínea Taveira de Jesus ◽  
Analúcia Guedes Silveira Cabral ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Gastroprotective Activity ◽  
Hexane Phase

scholarly journals Biomass to porous carbon in one step: directly activated biomass for high performance CO2 storage

2017 ◽  
Vol 5 (24) ◽  
pp. 12330-12339 ◽  
Author(s):  
Norah Balahmar ◽  
Abdul Salam Al-Jumialy ◽  
Robert Mokaya
Keyword(s):  
Porous Carbon ◽  
High Performance ◽  
Lower Cost ◽  
Activated Carbons ◽  
Co2 Storage ◽  
Direct Conversion ◽  
Step Process ◽  
One Step ◽  
Hydrothermal Carbonisation ◽  
Activated Biomass

The direct conversion of biomass to activated carbons in a simple and lower cost one step process, which negates the need for hydrothermal carbonisation or pyrolysis, generates activated carbons with properties and CO2 uptake comparable or superior to those of conventionally prepared activated carbons.

scholarly journals Antiproliferative Activity and Antioxidant Potential of Extracts of Garcinia gardneriana

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3201
Author(s):  
Simone da Cunha Demenciano ◽  
Magalli Costa Barbosa Lima e Silva ◽  
Caroline Almeida Farias Alexandrino ◽  
Wilson Hino Kato Junior ◽  
Patrícia de Oliveira Figueiredo ◽  
...  
Keyword(s):  
Vitamin C ◽  
Antiproliferative Activity ◽  
High Performance ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Array Detector ◽  
Chloroform Fraction ◽  
Chemical Profile ◽  
Polycarboxylic Acids

The aim of this study was to evaluate the antiproliferative activity, the antioxidant potential, and the chemical profile obtained from the whole fruit and from leaves of Garcinia gardneriana, a fruit tree from Brazilian Cerrado. To determine in vitro antiproliferative activity, the following neoplastic cell lines were considered, along with an immortalized nontumor cell line. The antioxidant potential was determined, and the evaluation of antiradical air activity was performed. The levels of vitamin C and carotenoids were determined. The chemical profile was analyzed by high-performance liquid chromatography coupled to a diode array detector and a mass spectrometer using electrospray ionization interface. The chloroform fraction of the leaf showed antioxidant activity. The vitamin C content had lower values in fruits and higher in leaves. The content of carotenoids for fruits and leaves was expressive. The ethanolic extract and the hexane and chloroform fractions of fruits were active in all neoplastic lines tested. The leaves showed cytotoxic activity in the hexane fraction in the breast carcinoma line. The analysis of data obtained verified the presence of dimers, monomers, and tetramers of hexoses, polycarboxylic acids, xanthones, flavonoids, biflavonoids, and benzophenones.

scholarly journals Recovery of Bioactive Compounds from Hazelnuts and Walnuts Shells: Quantitative–Qualitative Analysis and Chromatographic Purification

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1363 ◽  
Author(s):  
René Herrera ◽  
Jarl Hemming ◽  
Annika Smeds ◽  
Oihana Gordobil ◽  
Stefan Willför ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Antioxidant Capacity ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Short Chain Fatty Acids ◽  
Extraction Process ◽  
Size Exclusion ◽  
Flash Chromatography ◽  
Layer Chromatography

Hazelnut (HS) and walnut (WS) shells, an abundant by-product of the processing industries of these edible nuts, are traditionally considered as a low-value waste. However, they are a source of valuable compounds with an interesting chemical profile for the chemical and pharmaceutical sectors. In this study, the lipophilic and hydrophilic extracts present in HS and WS were quantified and identified, then the polar fractions were chromatographically separated, and their antioxidant capacity was studied. The experimental work includes the isolation of crude lipophilic and hydrophilic extracts by an accelerated extraction process, chromatographic analysis (gas chromatography-flame ionization (GC-FID), GC-mass spectroscopy (GC-MS), high-performance size-exclusion chromatography (HPSEC), thin-layer chromatography (TLC)), and quantification of the components. In addition, a thorough compositional characterization of the subgroups obtained by flash chromatography and their antioxidant capacity was carried out. The gravimetric concentrations showed different lipophilic/hydrophilic ratios (0.70 for HS and 0.23 for WS), indicating a higher proportion of polar compounds in WS than in HS. Moreover, the lipophilic extracts were principally composed of short-chain fatty acids (stearic, palmitic, and oleic acid), triglycerides, and sterols. The polar fractions were screened by thin-layer chromatography and then separated by flash chromatography, obtaining fractions free of fatty acids and sugar derivatives (97:3 in HS and 95:5 in WS), and mixtures richer in phenolic compounds and flavonoids such as guaiacyl derivatives, quercetin, pinobanksin, and catechin. The most polar fractions presented a higher antioxidant capacity than that of the crude extracts.

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