Effect of berberine on the ratio of high-molecular weight adiponectin to total adiponectin and adiponectin receptors expressions in high-fat diet fed rats

2016 ◽  
Author(s):  
Yue-yue Wu ◽  
Ying Zha ◽  
Jun Liu ◽  
Fang Wang ◽  
Jiong Xu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Fat Diet ◽  
Adiponectin Receptors ◽  
High Fat ◽  
High Molecular Weight Adiponectin ◽  
Total Adiponectin

scholarly journals Linoleic Acid–Rich Oil Supplementation Increases Total and High-Molecular-Weight Adiponectin and Alters Plasma Oxylipins in Postmenopausal Women with Metabolic Syndrome

2020 ◽  
Vol 4 (9) ◽  
Author(s):  
Rachel M Cole ◽  
Sarah Puchala ◽  
Jia-Yu Ke ◽  
Mahmoud Abdel-Rasoul ◽  
Kristin Harlow ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Molecular Weight ◽  
Linoleic Acid ◽  
High Molecular Weight ◽  
Oil Consumption ◽  
Open Label ◽  
High Molecular Weight Adiponectin ◽  
Markers Of Inflammation ◽  
Hmw Adiponectin ◽  
Total Adiponectin

ABSTRACT Background The onset of menopause increases the risk of metabolic syndrome (MetS). Adiponectin is an adipokine associated with insulin sensitivity that is lower in people with MetS. Supplementing diets with linoleic acid (LA)-rich oil increased adiponectin concentrations and improved glucose control in women with type 2 diabetes. The effect of LA on adipokines, especially total and the bioactive form of adiponectin, high-molecular-weight (HMW) adiponectin, in women with MetS is unknown. Objectives The aim of this study was to explore the effect of supplementation of the diet with an oil rich in LA on adipokines in women with MetS. The effect of the LA-rich oil (LA-oil) on oxylipins, key metabolites that may influence inflammation and metabolism, was also explored. Methods In this open-label single-arm pilot study, 18 postmenopausal nondiabetic women with MetS enrolled in a 2-phase study were instructed to consume LA-rich vegetable oil (10 mL/d) as part of their habitual diets. Women consumed an oleic acid–rich oil (OA-oil) for 4 wk followed by an LA-oil for 16 wk. Fasting concentrations of adipokines, fatty acids, oxylipins, and markers of glycemia and inflammation were measured. Results After 4 wk of OA-oil consumption, fasting glucose and total adiponectin concentrations decreased whereas fasting C-reactive protein increased. After 16 wk of LA-oil supplementation total and HMW adiponectin and plasma oxylipins increased. Markers of inflammation and glycemia were unchanged after LA-oil consumption. Conclusions Supplementation with LA-oil increased total and HMW adiponectin concentrations and altered plasma oxylipin profiles. Larger studies are needed to elucidate the links between these changes and MetS. This trial was registered at clinicaltrials.gov as NCT02063165.

scholarly journals High Molecular Weight, Rather than Total, Adiponectin Levels Better Reflect Metabolic Abnormalities Associated with Childhood Obesity

2006 ◽  
Vol 91 (12) ◽  
pp. 5113-5116 ◽  
Author(s):  
Shunsuke Araki ◽  
Kazushige Dobashi ◽  
Kazuyasu Kubo ◽  
Kohtaro Asayama ◽  
Akira Shirahata
Keyword(s):  
Metabolic Syndrome ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
University Hospital ◽  
Metabolic Abnormalities ◽  
Obese Children ◽  
High Molecular Weight Adiponectin ◽  
Visceral Fat Area ◽  
Metabolic Derangement ◽  
Total Adiponectin

Abstract Context: Japanese are prone to obesity-induced metabolic derangement, which is linked to serum adipocytokine profile even in children. Objective: The objective of the study was to determine whether high molecular-weight adiponectin (H-Adn) more specifically relates to metabolic derangement in obese children than total adiponectin (T-Adn). Design and Setting: A case (n = 59) control (n = 28) study was performed at the pediatric clinic of a university hospital. Patients: Japanese obese children (38 boys and 21 girls) were consecutively enrolled. The ages ranged from 5 to 15 (10.3 ± 0.3; mean ± sem) yr. Nonobese children (15 boys and 13 girls) were assigned as age-matched controls. Main Outcome Measures: Serum adiponectin multimeric complexes were assayed by an ELISA kit. The relationship of adiponectin to metabolic abnormalities was evaluated. Results: T-Adn (5.1 ± 0.2 vs. 8.8 ± 0.4 μg/ml), H-Adn (1.3 ± 0.1 vs. 4.8 ± 0.4 μg/ml), and medium molecular weight-Adn were significantly lower in obese than in control children. After adjustment for age and sex, both T- and H-Adn were inversely correlated with insulin and homeostasis model of assessment-insulin resistance, whereas H-Adn (but not T-Adn) inversely correlated with visceral fat area, as determined by computed tomography. Seven obese children were estimated to have metabolic syndrome and showed selective decrease in H-Adn and H/T-Adn. Conclusion: H-Adn reflects metabolic abnormalities due to obesity better than T-Adn in children. H-Adn is associated with the development of metabolic syndrome, even in childhood.

Measurement of high-molecular-weight adiponectin is not useful in assessing coronary stenosis

2012 ◽  
Vol 50 (4) ◽  
Author(s):  
Takuya Imatoh ◽  
Motonobu Miyazaki ◽  
Ken Kadowaki ◽  
Shinichi Tanihara ◽  
Chinami Akashi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Coronary Stenosis ◽  
Active Form ◽  
Surrogate Marker ◽  
Area Under The Curve ◽  
High Molecular Weight Adiponectin ◽  
Cross Sectional ◽  
Hmw Adiponectin ◽  
Total Adiponectin

AbstractIn many studies, high-molecular-weight (HMW) adiponectin has been considered the active form of adiponectin. However, whether HMW adiponectin is a good surrogate marker for coronary artery disease still needs to be elucidated.We conducted a hospital-based cross-sectional study to examine the relationship between total, HMW or non-HMW adiponectin concentrations and coronary stenosis in 83 male patients and 138 male controls.Patients with coronary stenosis had significantly lower total adiponectin concentrations compared with controls. Non-HMW adiponectin concentrations in cases were significantly lower than the controls. However, there were no significant differences between cases and controls in HMW adiponectin concentrations. From the receiver operating characteristic (ROC) analysis, the area under the curve (AUC) for total and non-HMW adiponectin was significantly larger than that for HMW adiponectin concentrations. Of the three models, that for non-HMW adiponectin showed the largest AUC (total adiponectin 0.74, HMW adiponectin 0.54, and non-HMW adiponectin 0.79).Despite associations between total adiponectin levels and coronary stenosis, our data go against any apparent association between HMW adiponectin concentrations and coronary stenosis.

scholarly journals Collagen beta (1-O) galactosyltransferase 1 (GLT25D1) is required for the secretion of high molecular weight adiponectin and affects lipid accumulation

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Julie A. Webster ◽  
Zhe Yang ◽  
Yu-Hee Kim ◽  
Dorothy Loo ◽  
Rasha M. Mosa ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Lipid Accumulation ◽  
Post Translational Modification ◽  
High Fat ◽  
High Molecular Weight Adiponectin ◽  
Human Embryonic Kidney Cells ◽  
Hmw Adiponectin ◽  
Hek Cells ◽  
Sgbs Cells

Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. The present study aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its significance in relation to obesity. Collagen beta (1-O) galactosyltransferase 1 (GLT25D1) was knocked down in HEK cells modified for the stable expression of adiponectin (adiponectin expressing human embryonic kidney cells, Adipo-HEK) as well as in Simpson Golabi-Behmel-Syndrome (SGBS) adipocytes. Knockdown of GLT25D1 caused a significant decrease in HMW adiponectin in Adipo-HEK cells with no change in total adiponectin. Knockdown in the SGBS cells caused an increase in lipid accumulation yet inhibited adipogenesis. Co-immunoprecipitation with adiponectin and mass spectrometry showed that adiponectin formed a protein complex with lysyl hydroxylase 3 (LH3) and GLT25D1. Transient overexpression of GLT25D1 showed that the intracellular retention of LH3 was dependent on GLT25D1. To determine whether changes in GLT25D1 were significant in obesity, mice were fed a standard chow or high-fat diet (HFD) for 5 weeks. GLT25D1 was significantly decreased in mice fed HFD which coincided with a decrease in HMW adiponectin. We conclude that GLT25D1 regulates HMW adiponectin secretion and lipid accumulation, consistent with changes in mice after high-fat feeding. These results suggest a novel function of GLT25D1 leading to decreased HMW adiponectin secretion in early obesity.

Persimmon Tannin accounts for hypolipidemic effects of persimmon through activating of AMPK and suppressing NF-κB activation and inflammatory responses in High-Fat Diet Rats

2014 ◽  
Vol 5 (7) ◽  
pp. 1536-1546 ◽  
Author(s):  
Bo Zou ◽  
Zhen-zhen Ge ◽  
Ying Zhang ◽  
Jing Du ◽  
Ze Xu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Fat Diet ◽  
Inflammatory Responses ◽  
Central Component ◽  
High Fat ◽  
Ampk Pathway

High molecular weight persimmon tannin is a central component accounting for the anti-hyperlipidemic effects of consuming persimmon fruits via AMPK pathway.

scholarly journals A prospective study of circulating adipokine levels and risk of multiple myeloma

Blood ◽  
2012 ◽  
Vol 120 (22) ◽  
pp. 4418-4420 ◽  
Author(s):  
Jonathan N. Hofmann ◽  
Linda M. Liao ◽  
Michael N. Pollak ◽  
Ye Wang ◽  
Ruth M. Pfeiffer ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Multiple Myeloma ◽  
High Molecular Weight ◽  
Blood Collection ◽  
Ovarian Cancer Screening ◽  
High Molecular Weight Adiponectin ◽  
Total Adiponectin ◽  
Polypeptide Hormones ◽  
A Prospective Study ◽  
Circulating Levels

Abstract It has been hypothesized that the observed excess risk of multiple myeloma (MM) among obese persons could be the result of altered circulating levels of adipokines, polypeptide hormones with pro- and anti-inflammatory properties secreted by adipose tissue. We investigated whether circulating levels of leptin, total adiponectin, and high molecular weight adiponectin are associated with subsequent MM risk among 174 MM patients and 348 controls within the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Inverse associations with MM were observed for total adiponectin (highest quartile vs lowest: odds ratio = 0.49; 95% CI = 0.26-0.93, Ptrend = .03) and high molecular weight adiponectin (0.44; 0.23-0.85, Ptrend = .01). These associations remained after restricting to MM patients diagnosed ∼ 8 years or more after blood collection. Leptin levels were not associated with MM risk. The results of this study, to our knowledge the first prospective investigation of circulating adipokines and MM, suggest that adiponectin may play an important role in obesity-related myelomagenesis.

scholarly journals Circulating high-molecular-weight adiponectin is upregulated in preeclampsia and is related to insulin sensitivity and renal function

2008 ◽  
Vol 158 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Mathias Fasshauer ◽  
Theresa Waldeyer ◽  
Jeannette Seeger ◽  
Susanne Schrey ◽  
Thomas Ebert ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Renal Function ◽  
Insulin Sensitivity ◽  
High Molecular Weight ◽  
Active Form ◽  
Biologically Active ◽  
Serum Concentrations ◽  
High Molecular Weight Adiponectin ◽  
Hmw Adiponectin ◽  
Total Adiponectin

ObjectivePreeclampsia (PE) is a serious cardiovascular complication in pregnancy which is associated with an increased future metabolic and cardiovascular risk for mother and newborn. Recently, a paradoxical upregulation of the insulin-sensitizing and anti-atherogenic adipokine adiponectin has been shown in PE. Furthermore, high-molecular-weight (HMW) adiponectin has been suggested as the biologically active form of this adipokine.Design and methodsHMW adiponectin and total adiponectin serum concentrations were quantified by ELISA in PE (n=16) patients and pregnant control women without PE (n=20). Furthermore, HMW adiponectin and total adiponectin were correlated to clinical and biochemical measures of renal function, glucose, and lipid metabolism, as well as inflammation.ResultsMedian maternal HMW adiponectin and total adiponectin levels were significantly and independently upregulated almost twofold in PE when compared with controls. HMW adiponectin and total adiponectin correlated positively with creatinine and negatively with fasting insulin in univariate and multivariate analyses.ConclusionsWe show that maternal HMW adiponectin and total adiponectin serum concentrations are significantly increased in PE and are positively associated with markers of insulin sensitivity and renal dysfunction. Adiponectin might be part of a physiological feedback mechanism improving insulin sensitivity and cardiovascular health in PE.

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