Effects of UVB Light, Wounding Stress, and Storage Time on the Accumulation of Betalains, Phenolic Compounds, and Ascorbic Acid in Red Prickly Pear (Opuntia ficus-indica cv. Rojo Vigor)

2018 ◽  
Vol 11 (12) ◽  
pp. 2265-2274 ◽  
Author(s):  
Erika Ortega-Hernández ◽  
Jorge Welti-Chanes ◽  
Daniel A. Jacobo-Velázquez
2019 ◽  
Vol 20 (21) ◽  
pp. 5327 ◽  
Author(s):  
Ortega-Hernández ◽  
Nair ◽  
Welti-Chanes ◽  
Cisneros-Zevallos ◽  
Jacobo-Velázquez

The present study evaluated the effects of ultraviolet B (UVB) radiation and wounding stress, applied alone or combined, on the biosynthesis of phenolic compounds and ascorbic acid in the peel and pulp of red prickly pear (Opuntia ficus-indica cv. Rojo Vigor). Whole and wounded-fruit samples were treated with UVB radiation (6.4 W·m−2) for 0 and 15 min, and stored for 24 h at 16 °C. Phytochemical analyses were performed separately in the peel and pulp. The highest phenolic accumulation occurred after storage of the whole tissue treated with UVB, where the main phenolic compounds accumulated in the peel and pulp were quercetin, sinapic acid, kaempferol, rosmarinic acid, and sinapoyl malate, showing increases of 709.8%, 570.2%, 442.8%, 439.9%, and 186.2%, respectively, as compared with the control before storage. Phenylalanine ammonia-lyase (PAL) activity was increased after storage of the whole and wounded tissue treated with UVB light, and this increase in PAL activity was associated to phenolic accumulation. On the other hand, l-galactono-γ-lactone dehydrogenase (GalLDH) activity and ascorbic acid biosynthesis was enhanced due to UVB radiation, and the effect was increased when UVB was applied in the wounded tissue showing 125.1% and 94.1% higher vitamin C content after storage when compared with the control. Respiration rate was increased due to wounding stress, whereas ethylene production was increased by wounding and UVB radiation in prickly pears. Results allowed the generation of a physiological model explaining the UVB and wound-induced accumulation of phenolic compounds and ascorbic acid in prickly pears, where wounding facilitates UVB to access the underlying tissue and enhances an apparent synergistic response.


2008 ◽  
Vol 14 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Guang-Yuan Zhao ◽  
Wei Zong ◽  
Guang-Jie An

Cloud stability of particles in cloudy apple juice during its storage for 120 days at 4, 22 and 40 °C is conducted. The juices are added with or without ascorbic acid. The cloud stability of juices during storage is studied by optical microscopy, electron microscopy and size distribution analysis of suspending particles combined with a polyphenols component analysis by high performance liquid chromatography. At the beginning of storage, the turbidity of juice added with or without ascorbic acid decreased quickly because particles of larger size in juices aggregated and deposited, and since there are less larger particles at the upper portion of the bottles, the depositing speed of particles decreases. The loss of total phenolic compounds, epicatechin, chlorogenic acid and tannin increases with the increase of temperature and storage time. The polyphenols degraded quickly during the initial period of storage and then it slowed down. The addition of ascorbic acid to the juice can inhibit the oxidation and polymerisation of phenolic compounds to avoid the formation of new small insoluble particles during the storage, and the efficacy of inhibiting between adding 0.024% or 0.006% (w/w) ascorbic acid is not remarkably different.


Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 860 ◽  
Author(s):  
Anna Grobelna ◽  
Stanisław Kalisz ◽  
Marek Kieliszek

In this study, we aimed to investigate the effect of processing methods and storage time on selected parameters of quality in the purees of blue honeysuckle berries. We investigated the content of bioactive compounds such as anthocyanins, L-ascorbic acid, and total polyphenols (TPs). We tested four processing methods and two varieties of blue honeysuckle berry (Lonicera caerulea L.var. Sineglaska and Volshebnica). The purees were analyzed immediately after production, and after two and four months of storage at 20 °C without exposure to light. According to our results, thermal treatment of the fruits for 5 min resulted in obtaining purees with a higher content of anthocyanins and TPs, but lower content of L-ascorbic acid. However, sieving fruits that were not thermally treated resulted in a higher content of L-ascorbic acid. Furthermore, purees prepared from the fruits of the Volshebnica cultivar were richer in bioactive compounds than that of purees prepared from the Sineglaska cultivar. In addition, the content of anthocyanins, L-ascorbic acid, and TPs decreased with storage time.


2010 ◽  
Vol 8 (1) ◽  
pp. 26
Author(s):  
E P Samodra ◽  
H Cahyono

<p>The research was conducted to investigate the effect of soaking meat of Peranakan Ongole cattle with ascorbic acid and storage duration at 50C on physical quality of meat. The experiment was done in Complete Randomised Design by factorial 3 x 3. First factor was ascorbic acid with 3 levels (0%, 5%, 1%), and second factor is storage duration with 3 levels (0, 5, and 6 days). Variables that were measured: tenderness, cooking loss, water holding capacity and pH. Data were analyzed by variance analysis and the significant result were tested by Duncan’s test. Tenderness average for ascorbic acid ranging between 1,60 – 1,62 kg/cm2, and for storage duration was increased from 1,83 to 1,38 kg/cm2. Cooking loss average for ascorbat acid ranged from 43,33 to 44,89%, and for storage duration increased from 40,00 to 47,00%. Water holding capacity average for ascorbic acid ranging between 32,88 – 33,65%, but for storage duration decreased from 34,61 to 32,32%. The pH average for ascorbic acid ranging from 5,99 to 6,16, and increased for storage duration from 6,43 to 5,84. It can be concluded that physical quality of beef cattle was not affected<br />bay ascorbic acid up to 1%, but was significantly decreased by storage duration (p&lt;0,05).</p><p>Key words: Pernakan Ongole meat, ascorbat acid, storage time</p>


2020 ◽  
Vol 9 (12) ◽  
pp. e19591210872
Author(s):  
Giovana Matias do Prado ◽  
Geraldo Arraes Maia ◽  
Raimundo Wilane de Figueiredo ◽  
Jorgiane Silva Severino Lima ◽  
Claisa Andrea Freitas Rabelo ◽  
...  

This work aimed to study beverages' storage stability based on cashew apple pulp and honey during 120 days. Three formulations were prepared: F1 (75% of cashew apple pulp and 25% of honey; F2 (60% of cashew apple pulp and 40% of honey) and F3 (50% of cashew apple pulp and 50% of honey). The formulation F1 showed the highest content of ascorbic acid after storage (95.74 mg.100mL). The pH did not show variation between the formulations studied. Soluble solids showed significant differences during storage and were consistent with the drink's honey content, ranging from 28.8 to 30.0 for F1, from 36.5 to 40.1 for F2, and 44.3 to 47.7 for F3. The content of carotenoids and phenolic compounds decreased during storage time. The preparation of beverages based on cashew apple and honey presented an alternative to adding value to the cashew apple pulp.


2021 ◽  
Vol 16 (1) ◽  
pp. 91-102
Author(s):  
F P H Gonzalez ◽  
V C Saucedo ◽  
R D Guerra ◽  
E J Suarez ◽  
H R M Soto ◽  
...  

Postharvest quality, quantification of betalains, phenolic compounds, and antioxidant activity of peel, pulp, and juice of fruits of three prickly pears (Opuntia ficus-indica L. Mill.) cultivars of Colegio de Postgraduados in México, were measured. The red and orange cultivars showed outstanding features of postharvest quality (size, texture, TSS and pulp and juice content) highest content of betalains and phenolic compounds. Therefore, highest antioxidant activity. In general, the highest content of bioactive compounds was detected in the peel, besides the content in pulp and juice did not show statistically significant differences. Phenolic content is very high in comparison with other fruits. Antioxidant activity was measured by three assays:FRAP, ABTS, and DPPH. Three cultivars showed a high correlation between antioxidant activity and phenolic compounds. The methodologies used in this work are a very useful tool for the quantification of bioactive compounds in O. ficus-indica fruit tissues.


2021 ◽  
Vol 11 (22) ◽  
pp. 10951
Author(s):  
Juan Carlos Sánchez-Rangel ◽  
Jorge Benavides ◽  
Daniel A. Jacobo-Velázquez

Carrot pomace is the main waste residue obtained during carrot juice extraction. Plant tissues respond to abiotic stresses (i.e., wounding stress and ultraviolet C (UVC) radiation) by accumulating bioactive compounds. Due to the mechanical damage occurring during juice extraction, carrot pomace undergoes extreme wounding stress. In this study, the effects of UVC light (11.8 W m−2, 0–120 min) and storage time (48 h, 25 °C) on the accumulation of phenolics compounds and the antioxidant activity (AOX) of carrot pomace were evaluated. Carrot pomace that was non-treated with UVC (control) showed a 709.5% increase in total phenolics at 48 h. A high correlation of AOX values against total phenolics (R2 = 0.87) was observed, indicating that phenolics were the main contributors to the AOX of the tissue. After UVC treatment, the pomace that was radiated for 120 min with UVC showed an increase (40.4%) in chlorogenic acid (CHA) content. At 24 h, protocatechuic acid and 3,5-dicaffeoylquinic acid, which were not detected before storage, showed accumulation by 166.5 mg/kg and 169.4 mg/kg, respectively, in UVC treated pomace. Chlorogenic acid showed the highest increase (143.6%) at 48 h in the control. Valorization of carrot pomace was achieved by increasing its concentration of antioxidant phenolics through UVC radiation.


2017 ◽  
Vol 42 (2) ◽  
pp. e13437 ◽  
Author(s):  
Fahad Al Juhaimi ◽  
Mehmet Musa Özcan ◽  
Nurhan Uslu ◽  
Kashif Ghafoor ◽  
Elfadil E. Babiker

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