Surface Tension and Self-association Properties of Aqueous Polysorbate 20 HP and 80 HP Solutions: Insights into Protein Stabilisation Mechanisms

2020 ◽  
Author(s):  
Patrick Garidel ◽  
Michaela Blech ◽  
Julia Buske ◽  
Alfred Blume
Keyword(s):  
Surface Tension ◽  
Polysorbate 20 ◽  
Self Association ◽  
Protein Stabilisation

Assessment of Surface Tension and Viscosity of In-Zn melt

2017 ◽  
pp. 15-19 ◽  
Author(s):  
R. P. Koirala ◽  
S. K. Yadav ◽  
B. P. Singh ◽  
I. S. Jha ◽  
D Adhikari
Keyword(s):  
Surface Tension ◽  
Thermodynamic Properties ◽  
Comparative Study ◽  
Concentration Dependence ◽  
Layered Structure ◽  
Association Model ◽  
Structure Approach ◽  
Self Association ◽  
Zn Alloy ◽  
Zn Alloys

A comparative study of the concentration dependence of surface tension and viscosity of molten In-Zn alloys at 700 K has been performed using energetics, obtained from thermodynamic analysis in the framework of self-association model. The surface tension of the alloys has been calculated from the two methods, layered structure approach and self associating mixture method, and viscosity from Moelwyn-Hughes equation and Kaptay equation. The theoretically computed results for the thermodynamic properties of the alloys are found in excellent agreement with the corresponding experimental results showing that the alloy is segregating in nature. Both surface tension and viscosity of the In-Zn alloy are found to increase with the addition of Zn atoms.The Himalayan Physics Vol. 6 & 7, April 2017 (15-19)

scholarly journals The Glycolytic Protein Phosphofructokinase Dynamically Relocalizes into Subcellular Compartments with Liquid-like Properties in vivo

2019 ◽  
Author(s):  
SoRi Jang ◽  
Zhao Xuan ◽  
Ross C. Lagoy ◽  
Louise M. Jawerth ◽  
Ian Gonzalez ◽  
...  
Keyword(s):  
Surface Tension ◽  
Biophysical Properties ◽  
Energy Stress ◽  
Subcellular Compartments ◽  
Cellular Environment ◽  
Self Association ◽  
Cryptochrome 2 ◽  
Rate Limiting ◽  
Sustained Hypoxia

AbstractWhile much is known about the biochemical regulation of glycolytic enzymes, less is understood about how they are organized inside cells. Here we built a hybrid microfluidic-hydrogel device for use in Caenorhabditis elegans to systematically examine and quantify the dynamic subcellular localization of the rate-limiting enzyme of glycolysis, phosphofructokinase-1/PFK-1.1. We determine that endogenous PFK-1.1 localizes to distinct, tissue-specific subcellular compartments in vivo. In neurons, PFK-1.1 is diffusely localized in the cytosol, but capable of dynamically forming phase-separated condensates near synapses in response to energy stress from transient hypoxia. Restoring animals to normoxic conditions results in the dispersion of PFK-1.1 in the cytosol, indicating that PFK-1.1 reversibly organizes into biomolecular condensates in response to cues within the cellular environment. PFK-1.1 condensates exhibit liquid-like properties, including spheroid shapes due to surface tension, fluidity due to deformations, and fast internal molecular rearrangements. Prolonged conditions of energy stress during sustained hypoxia alter the biophysical properties of PFK-1.1 in vivo, affecting its viscosity and mobility within phase-separated condensates. PFK-1.1’s ability to form tetramers is critical for its capacity to form condensates in vivo, and heterologous self-association domain such as cryptochrome 2 (CRY2) is sufficient to constitutively induce the formation of PFK-1.1 condensates. PFK-1.1 condensates do not correspond to stress granules and might represent novel metabolic subcompartments. Our studies indicate that glycolytic protein PFK-1.1 can dynamically compartmentalize in vivo to specific subcellular compartments in response to acute energy stress via multivalency as phase-separated condensates.

Comparison of the thermodynamic and transport properties of lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) with LiClO4 and Bu4NBr in water at 25 °C

1997 ◽  
Vol 75 (11) ◽  
pp. 1608-1614 ◽  
Author(s):  
Gérald Perron ◽  
Dany Brouillette ◽  
Jacques E. Desnoyers
Keyword(s):  
Phase Diagram ◽  
Surface Tension ◽  
Heat Capacity ◽  
Tetrabutylammonium Bromide ◽  
Lithium Perchlorate ◽  
Mass Action ◽  
Volume Heat ◽  
Action Model ◽  
Self Association ◽  
Solid Liquid

Lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) is a promising electrolyte for lithium batteries. While it presents many interesting features in aprotic solvents (high solubility and conductivity, electrochemical stability, etc.), little is known about its properties in water. The solid–liquid phase diagram, surface tension, volume, heat capacity, and conductivity were therefore measured at 25 °C and compared with the same properties of typical hydrophilic (LiClO4) and hydrophobic (Bu4NBr) electrolytes. To help in the comparison, some of the literature data for LiClO4 and Bu4NBr were extended. The standard Λ0, [Formula: see text] and [Formula: see text] are respectively 71.12 S cm2 mol−1, 139.7 cm3 mol−1 and 543 J K−1 mol−1at 25 °C. LiTFSI s quite soluble, and shows surface activity and a small tendency for self-association in water. The aggregation number estimated from a mass-action model is of the order of 4. Keywords: lithium bis(trifluoromethylsulfonyl)imide, lithium perchlorate, tetrabutylammonium bromide, volume, heat capacity, phase diagram, thermal analysis, surface tension, conductance.

scholarly journals Targeted Control of Kinetics of β-Amyloid Self-association by Surface Tension-modifying Peptides

2003 ◽  
Vol 278 (42) ◽  
pp. 40730-40735 ◽  
Author(s):  
Jin Ryoun Kim ◽  
Todd J. Gibson ◽  
Regina M. Murphy
Keyword(s):  
Surface Tension ◽  
Β Amyloid ◽  
Self Association ◽  
Kinetics Of

NMR spectroscopy and surface tension measurements applied to the study of self-association of casopitant mesylate, a novel NK1 antagonist

2011 ◽  
Vol 54 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Stefano Provera ◽  
Stefania Beato ◽  
Zadeo Cimarosti ◽  
Lucilla Turco ◽  
Andrea Casazza ◽  
...  
Keyword(s):  
Surface Tension ◽  
Nmr Spectroscopy ◽  
Nk1 Antagonist ◽  
Self Association

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

The Critical Point Drying Method Applied to Scanning Electron Microscopy of L-929 Cells

1970 ◽  
Vol 28 ◽  
pp. 278-279
Author(s):  
Charles TurnbiLL ◽  
Delbert E. Philpott
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Surface Tension ◽  
Critical Point ◽  
Freeze Drying ◽  
Attractive Alternative ◽  
Crystal Formation ◽  
Critical Point Drying ◽  
Time Required ◽  
Scanning Electron

The advent of the scanning electron microscope (SCEM) has renewed interest in preparing specimens by avoiding the forces of surface tension. The present method of freeze drying by Boyde and Barger (1969) and Small and Marszalek (1969) does prevent surface tension but ice crystal formation and time required for pumping out the specimen to dryness has discouraged us. We believe an attractive alternative to freeze drying is the critical point method originated by Anderson (1951; for electron microscopy. He avoided surface tension effects during drying by first exchanging the specimen water with alcohol, amy L acetate and then with carbon dioxide. He then selected a specific temperature (36.5°C) and pressure (72 Atm.) at which carbon dioxide would pass from the liquid to the gaseous phase without the effect of surface tension This combination of temperature and, pressure is known as the "critical point" of the Liquid.

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