Comparative lipidomic analysis of Cephalosporium acremonium insights into industrial and pilot fermentations

AbstractIn recent months, Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world. COVID-19 patients show mild, moderate or severe symptoms with the latter ones requiring access to specialized intensive care. SARS-CoV-2 infections, pathogenesis and progression have not been clearly elucidated yet, thus forcing the development of many complementary approaches to identify candidate cellular pathways involved in disease progression. Host lipids play a critical role in the virus life, being the double-membrane vesicles a key factor in coronavirus replication. Moreover, lipid biogenesis pathways affect receptor-mediated virus entry at the endosomal cell surface and modulate virus propagation. In this study, targeted lipidomic analysis coupled with proinflammatory cytokines and alarmins measurement were carried out in serum of COVID-19 patients characterized by different severity degree. Serum IL-26, a cytokine involved in IL-17 pathway, TSLP and adiponectin were measured and correlated to lipid COVID-19 patient profiles. These results could be important for the classification of the COVID-19 disease and the identification of therapeutic targets.

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Lipidomic analysis of single and combined effects of polyethylene microplastics and polychlorinated biphenyls on human hepatoma cells

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2021.126777 ◽

2021 ◽

pp. 126777

Author(s):

Albert Menéndez-Pedriza ◽

Joaquim Jaumot ◽

Carmen Bedia

Keyword(s):

Polychlorinated Biphenyls ◽

Hepatoma Cells ◽

Combined Effects ◽

Human Hepatoma ◽

Human Hepatoma Cells ◽

Lipidomic Analysis

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Evaluation of two highly effective lipid-lowering therapies in subjects with acute myocardial infarction

Scientific Reports ◽

10.1038/s41598-021-95455-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Aline Klassen ◽

Andrea Tedesco Faccio ◽

Carolina Raissa Costa Picossi ◽

Priscilla Bento Matos Cruz Derogis ◽

Carlos Eduardo dos Santos Ferreira ◽

...

Keyword(s):

Myocardial Infarction ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipid Lowering ◽

Low Density Lipoprotein Cholesterol ◽

Residual Cardiovascular Risk ◽

St Segment Elevation ◽

Lipidomic Analysis ◽

St Segment ◽

Highly Effective

AbstractFor cardiovascular disease prevention, statins alone or combined with ezetimibe have been recommended to achieve low-density lipoprotein cholesterol targets, but their effects on other lipids are less reported. This study was designed to examine lipid changes in subjects with ST-segment elevation myocardial infarction (STEMI) after two highly effective lipid-lowering therapies. Twenty patients with STEMI were randomized to be treated with rosuvastatin 20 mg QD or simvastatin 40 mg combined with ezetimibe 10 mg QD for 30 days. Fasting blood samples were collected on the first day (D1) and after 30 days (D30). Lipidomic analysis was performed using the Lipidyzer platform. Similar classic lipid profile was obtained in both groups of lipid-lowering therapies. However, differences with the lipidomic analysis were observed between D30 and D1 for most of the analyzed classes. Differences were noted with lipid-lowering therapies for lipids such as FA, LPC, PC, PE, CE, Cer, and SM, notably in patients treated with rosuvastatin. Correlation studies between classic lipid profiles and lipidomic results showed different information. These findings seem relevant, due to the involvement of these lipid classes in crucial mechanisms of atherosclerosis, and may account for residual cardiovascular risk.Randomized clinical trial: ClinicalTrials.gov, NCT02428374, registered on 28/09/2014.

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CBIO-05. LIPID METABOLIC REPROGRAMMING SENSITIZES A MOLECULARLY-DEFINED SUBSET OF GLIOBLASTOMAS TO FERROPTOSIS

Neuro-Oncology ◽

10.1093/neuonc/noaa215.065 ◽

2020 ◽

Vol 22 (Supplement_2) ◽

pp. ii16-ii16

Author(s):

Danielle Morrow ◽

David Nathanson ◽

Timothy Cloughesy ◽

Robert Prins ◽

Nicholas Bayley ◽

...

Keyword(s):

Lipid Metabolism ◽

Lipid Droplets ◽

Metabolic Reprogramming ◽

Membrane Phospholipids ◽

Molecular Alterations ◽

Lipidomic Analysis ◽

Novel Association ◽

Therapeutic Opportunity ◽

Dependent Cell ◽

Main Components

Abstract Cancers, including the universally lethal glioblastoma (GBM), have reprogrammed lipid metabolism to fuel tumor growth. However, the molecular alterations responsible for aberrant lipid metabolism, and the potential for identifying new therapeutic opportunities are not fully understood. To systematically investigate the GBM lipidome, we performed integrated transcriptomic, genomic and shotgun lipidomic analysis of an extensive library of molecularly diverse patient-derived GBM samples. Using this comprehensive approach, we discovered two GBM sub-groups defined by their combined molecular and lipidomic profile. Triacylglycerides (TAGs) enriched in polyunsaturated fatty acids (PUFAs) were among the most significantly altered lipids between the two groups of GBM tumors. TAGs are the main components of lipid droplets, which sequester PUFA-TAGs away from membrane phospholipids where their peroxidation can lead to ferroptosis – a regulated from of PUFA-peroxidation dependent cell death. Accordingly, the GBM subgroup with a depletion of PUFA TAGs showed heightened sensitivity to ferroptosis. Our findings suggest a novel association between specific molecular signatures of GBM, lipid metabolism and ferroptosis. This relationship may present a new therapeutic opportunity to target reprogrammed lipid metabolism in a molecularly-defined subset of GBMs.

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Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients

Metabolites ◽

10.3390/metabo11070412 ◽

2021 ◽

Vol 11 (7) ◽

pp. 412

Author(s):

Martin Malý ◽

Martin Hajšl ◽

Kamila Bechyňská ◽

Ondřej Kučerka ◽

Martin Šrámek ◽

...

Keyword(s):

Oxidative Stress ◽

Acute Coronary Syndrome ◽

High Performance ◽

Coronary Syndrome ◽

Lipidomic Analysis ◽

Comprehensive Information ◽

Group A ◽

Fatty Acyls ◽

Group D ◽

Standard Marker

Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initiated by oxidation of low-density lipoproteins incorporated into the intima of the vessel wall. Here, we studied lipids in plasma samples from three cohorts: 61 patients with ACS (group A), 49 patients with AIS (group D), and 82 controls (group K). Untargeted lipidomics based on high-performance liquid chromatography coupled to mass spectrometry (UHPLC-HRMS) was employed to obtain comprehensive information on whether relationships exist between these patient categories based on lipid patterns. In addition, malondialdehyde (MDA) as a standard marker of oxidative stress was monitored. The most characteristic lipids in group K were fatty acyls of hydroxyfatty acids (FAHFAs). As expected, MDA concentrations were the lowest in group K. Our findings can better explain ongoing pathologies, both acute and chronic, with the potential for future diagnosis and treatment.

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