Isolation, Characterization and Biosafety Evaluation of Lactobacillus Fermentum OK with Potential Oral Probiotic Properties

2021 ◽  
Author(s):  
Soyon Mann ◽  
Myeong Soo Park ◽  
Tony V. Johnston ◽  
Geun Eog Ji ◽  
Keum Taek Hwang ◽  
...  
Keyword(s):  
Lactobacillus Fermentum ◽  
Probiotic Properties ◽  
Biosafety Evaluation

scholarly journals ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF Lactobacillus fermentum EIPW5A ISOLATED FROM WHEY

2021 ◽  
pp. e248
Author(s):  
Dipanwita Bhattacharjee ◽  
Barun Bhattacharyya
Keyword(s):  
Lactic Acid Production ◽  
Probiotic Strain ◽  
16S Rrna Gene Sequencing ◽  
Lactobacillus Fermentum ◽  
Resistance Pattern ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Natural Habitats ◽  
Acid And Bile Tolerance ◽  
Rrna Gene Sequencing

The probiotic organisms are now used widely for different clinical indications. In an attempt to isolate a good probiotic strain for therapeutic applications, we have screened several isolates having probiotic attributes. The essential probiotic characters such as lactic acid production, antimicrobial activity, acid and bile tolerance, vitamin B12 production and antibiotic resistance pattern were considered as parameters for screening of probiotic bacteria from its natural habitats. Considering the said probiotic properties the strain EIPW5A was selected for the present study. The organism was identified as Lactobacillus fermentum based on its morphological, biochemical, physiological characters and 16S rRNA gene sequencing results.

Safety and efficacy of human breast milk Lactobacillus fermentum CECT 5716. A mini-review of studies with infant formulae

2015 ◽  
Vol 6 (2) ◽  
pp. 219-224 ◽  
Author(s):  
E. López-Huertas
Keyword(s):  
Breast Milk ◽  
Probiotic Strain ◽  
Lactobacillus Fermentum ◽  
Controlled Trials ◽  
Human Breast Milk ◽  
Probiotic Properties ◽  
Human Breast ◽  
Safety And Efficacy

Human breast milk has been described as a source of lactic acid bacteria. Lactobacillus fermentum CECT5716 is a human breast milk strain whose probiotic properties, safety and efficacy has been demonstrated in vitro and in vivo, including controlled trials with human adults. Since the origin of this probiotic strain is human breast milk, we aimed to investigate the safety and efficacy of an infant and a follow-on formulas supplemented with this strain of L. fermentum. We carried out two randomised controlled trials: one trial with infants of 6-12 months of age (follow-on formula study) and another one with infants from 1 to 5 months of age (infant formula study). The results from the trials showed that the probiotic formulas were safe, well tolerated and might be useful for the prevention of community-acquired infections.

Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 695-701 ◽  
Author(s):  
Yan Bao ◽  
Yanchao Zhang ◽  
Yong Zhang ◽  
Yong Liu ◽  
Shuiquan Wang ◽  
...  
Keyword(s):  
Dairy Products ◽  
Lactobacillus Fermentum ◽  
Probiotic Properties ◽  
Traditional Dairy Products

scholarly journals Lactobacillus fermentum strains from rice water and lemon pickle with potential probiotic properties and wastewater treatment applications

2020 ◽  
Author(s):  
Vidhya Prakash ◽  
Archana Palillam Veedu Palillam Veedu ◽  
Pradeesh Babu ◽  
Abhirami Jothish ◽  
Sruthy S Nair ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Klebsiella Pneumoniae ◽  
Salmonella Enterica ◽  
Low Ph ◽  
Enteric Pathogens ◽  
Lactobacillus Fermentum ◽  
Food Sources ◽  
Probiotic Properties ◽  
E Coli ◽  
Rice Water

Abstract Enteric infection by faecal contamination of drinking water is a major concern in developing nations contributing to the huge burden of malnutrition and infant mortality. We have developed a holistic approach, named as gut to gutter approach, to treat wastewater treatment, by screening potential probiotic strains from staple food sources. The strains could be potentially used for competing out the enteric pathogens in different applications such as food and wastewater treatment. We focused on two strains isolated from rice water (RS) and lemon pickle (T1), identified as Lactobacillus fermentum and characterized for their probiotic and wastewater treatment capabilities. The strains showed many probiotic properties (tolerance to low pH (pH 3), bile salts up to 0.5% and simulated gastric juice at low pH and binding to extracellular matrix molecules, heparin and gelatin. Auto-aggregation of T1 was in the range of 85% and the strain exhibited significant co-aggregating ability with enteric pathogens, Klebsiella pneumoniae, Salmonella enterica and E. coli (MDR) with 48%, 79% and 65% respectively. Both strains had a higher binding affinity to gelatin and heparin, compared to widely claimed “probiotic” Bacillus clausii and the pathogenic E. coli ET. The strains had high galactosidase activities. Further, the cell free supernatant from RS showed BLIS (bacteriocin like inhibitory substance) activity against Klebsiella pneumoniae, Staphylococcus aureus and Salmonella enterica at 60%, 48% and 30% respectively. T1 strains and B. clausii effectively reduced the coliform count by by 90% (1-log) when immobilized in a biofilter to treat wastewater.

scholarly journals Profiling of Microbial Content and Growth in Fermented Maize Based Products from Western Kenya

2018 ◽  
Vol 6 (2) ◽  
pp. 509-519
Author(s):  
Herve Mwizerwa ◽  
George Ooko Abong ◽  
Samuel Kuria Mbugua ◽  
Michael Wandayi Okoth ◽  
Patrick Gacheru ◽  
...  
Keyword(s):  
Starter Culture ◽  
Titratable Acidity ◽  
Viable Count ◽  
Lactobacillus Fermentum ◽  
Probiotic Properties ◽  
Maize Flour ◽  
Fermentation Time ◽  
Western Kenya ◽  
Cereal Products ◽  
Molecular Tests

In most parts of Africa, the process of fermentation is not controlled and does not adhere to good manufacturing practices, therefore spoilage and pathogenic microorganisms can alter the quality of the end product and may cause foodborne illness.Traditional fermented products are mostly processed in an environment which creates a selection of microorganisms that produce the desired end product. In an attempt to find Lactobacilli which have probiotic properties and can be used in the development of starter culture for controlled fermentation of cereal products, the microbial populations of maize flour, overnight soaked dough, fermented cooked porridge, Mkarango and Busaawere enumerated and the inherent lactobacilli isolated. The microbial and biochemical profiles of the 6 days spontaneous Mkarangofermentation process were determined.The total viable count was 6.93 log cfu/g for fermented cooked porridge, 7.70 log cfu/g in Mkarango and 8.58 log cfu/g forBusaa. Lactobacillicounts were higher in maize flour with 7.43 log cfu/g whileEnterobactericeae were lower in Mkarango.The highest moulds and yeasts counts were observed forBusaa, 7.25 log cfu/g.The lactobacilli isolates from fermented maize based products from western Kenya were predominantly Lactobacillus fermentum andLactobacillus Plantarum.During fermentation time, Lactobacilliincreased from 6.62 to 12.46 log cfu/gafter 3 daysof fermentation. From day 4, an increase in moulds and yeast count was observed, varying from 8.42 to 10.53 log cfu/g. Enterobactericeae count decreased from 5.99 log cfu/g on day 1 to less than 1 log cfu/g on day 6.Titratable acidity increased from 0.32% to 0.73% on day 5. Inversely, the pH of Mkarangodecreased sharply from 6.64 to 3.64 on day 5 and slightly increased on the last day of fermentation. The microbial status of finished fermented maize based products is predominated by Lactobacilli and their isolates are predominantly Lactobacilli especially Lactobacillus fermentum andLactobacillus Plantarumthough further molecular tests are needed to confirm the species.

scholarly journals IN VITRO STUDY ON RELEASE OF BIOACTIVE ANTIMICROBIAL COMPOUNDS FROM DAIRY PRODUCTS BY CERTAIN PROMISING PROBIOTIC LACTOBACILLUS STRAINS

2017 ◽  
Vol 9 (4) ◽  
pp. 27 ◽  
Author(s):  
Debapriya Mohanty ◽  
Manish Ranjan Saini ◽  
Swati Mohapatra
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Mass ◽  
Cell Surface Hydrophobicity ◽  
Bioactive Compound ◽  
Lactobacillus Fermentum ◽  
Diffusion Method ◽  
Bile Salt Hydrolase ◽  
Probiotic Properties ◽  
Antimicrobial Compound ◽  
Probiotic Lactobacillus

Objective: The antimicrobial activity and Probiotic properties of Lactobacillus species were evaluated. The antimicrobial compound of potent antimicrobial probiotic Lactobacillus was purified by column chromatography and its nature and stability were determined.Methods: This investigation was performed with few Lactobacillus strains of ATCC and MTCC along with certain strains isolated from different dairy sources. They were evaluated for their probiotics properties (acid tolerance, bile tolerance, bile salt hydrolase activity, cell autoaggregation, cell surface hydrophobicity and haemolytic behaviour). Agar well diffusion method was used to screen their potency to release bioactive compound against several pathogens. This potent antimicrobial compound was purified by chromatography as well as its molecular mass was estimated by following SDS-PAGE. Finally, the stability of the compound was determined against various ranges of temperature and pH.Results: Among all Lactobacillus strains, R1 was found to be a potent probiotic strain as well as cell free supernatant (CFS) of R1 showed more strong antagonistic effect against most of the pathogens. Carbohydrate fermentation and physiological characterization of strain R1 matched with Lactobacillus fermentum as per Bergey’s manual of systematic bacteriology. The molecular mass of the purified fraction was estimated at approximately 25 kDa and could be stable after heat treatment of 100 °C for 30 min and pH range of 4.5-7.0Conclusion: R1 showed highest antimicrobial activity while it has been found as Lactobacillus fermentum. It is due to the release of a bioactive compound having a molecular mass of 25 kDa. 

Molecular identification of bile tolerant Lactobacillus fermentum strains isolated from Human Faeces

2016 ◽  
Vol 1 (02) ◽  
pp. 182-189
Author(s):  
M. Kumar ◽  
P. K. Sharma ◽  
P. K. Sharma ◽  
N. Kumar
Keyword(s):  
Pathogenic Bacteria ◽  
Molecular Methods ◽  
Lactobacillus Fermentum ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Phenotypic Characteristics ◽  
Antimicrobial Substances ◽  
Polymerase Chain ◽  
Specific Part

Conventional procedures of identification are laborious and time consuming because they depend on growth of microorganism in that particular environment and hence, sometimes, give misleading results while molecular methods relies upon generating a large number of copies of a specific part of its genome for identification of unknown isolates and enables the problem of poor identification. The identification of a microbial isolate to genus level imparts a partial characterization of the isolate revealing a lot of information about that organism. Lactic acid bacteria are very significant to human health due to their probiotic attributes, production of antimicrobial substances and subsequently to inhibit pathogenic bacteria. Bile tolerance has been one of the major probiotic criterion while selecting the isolate for probiotic properties. This work aims at looking directly at the genome of bile tolerant Lactobacillus isolates using genotypic and phenotypic characteristics. The isolates were identified by sequencing specific region of 16 S r DNA segment, after amplification by PCR, and then comparing the results to sequences from related database. The results from both conventional and molecular methods were then compared. Twenty five isolates (25) of Lactobacillus fermentum were isolated from fecal samples and identified using API- 50 CH. The isolates were further identified using molecular methods using polymerase chain reaction (PCR) for amplification of 16S rRNA gene to confirm their identities. The genotypic characterization however showed that 84% of the organisms identified using conventional method as L. fermentum correlated, while 16% did not correlate; 2 were identified as Lactobacillus planterum, 01 as L. delbrueckiiand 01 as L.paraplanterum.

Sign in / Sign up

Export Citation Format

Share Document