Does Azospirillum brasilense and biostimulant improve the initial growth of rice sown at greater depths?

2020 ◽  
Vol 23 (5) ◽  
pp. 461-468
Author(s):  
Carlos Eduardo da Silva Oliveira ◽  
Tiago Zoz ◽  
Eduardo Pradi Vendruscolo ◽  
Agner de Freitas Andrade ◽  
Cássio de Castro Seron ◽  
...  
2019 ◽  
Vol 6 (3) ◽  
pp. 53-59 ◽  
Author(s):  
Gabriel Felipe Vogel ◽  
Rubens Fey

The application of biological products containing diazotrophic bacteria in the seed has been highlighted as an alternative to reduce the use of nitrogen fertilizers; however, the chemical treatment of the seeds may negatively affect the survival rate of these microorganisms in the soil. The effects of the application of captan (fungicide) and thiodicarb (insecticide) in association with the inoculation of Azospirillum brasilense were investigated in this study for the corn crop. The research was conducted under greenhouse conditions at Universidade Estadual do Centro-Oeste (UNICENTRO), in Guarapuava, Paraná, Brazil, and under laboratory conditions at the Universidade Federal da Fronteira Sul (UFFS), in Laranjeiras do Sul, Paraná, Brazil. A completely randomized experimental design with eight treatments and four replicates was used. The treatments were as follows: control; A. brasilense; captan; thiodicarb; captan + thiodicarb; A. brasilense + captan; A. brasilense + thiodicarb; A. brasilense + captan + thiodicarb. The germination and seedling growth rates were evaluated in the laboratory. In the greenhouse were measured the growth of the roots and shoots until the V8 corn growth stages. The use of A. brasilense alone resulted in higher plant growth when compared to the control treatment, whereas the association of A. brasilense with captan and thiodicarb resulted in a similar response to the uninoculated and control treatments. These results suggest that there was a possible toxic effect of seed treatment with these products. The use of captan and thiodicarb interfere in the interaction of diazotrophic bacteria with root growth, affecting the germination rate and initial corn growth.


2017 ◽  
pp. 1242-1248 ◽  
Author(s):  
Elisa Pereira de Oliveira ◽  
Matheus Gustavo da Silva ◽  
Paulo Eduardo Teodoro

Author(s):  
Osvaldo Matsuo ◽  
Claudemir Zucareli ◽  
Eduardo H. Horácio ◽  
Luiz A. R. Alves ◽  
Otavio J. G. A. Saab

ABSTRACT Although the isolated effects of the cyanobacteria Anabaena cylindrica and the bacteria Azospirillum brasilense are well-known, the co-inoculation of both can promote other benefits to corn plants’ biological nitrogen fixation and growth. The aim was to evaluate the initial growth and chloroplast pigment levels of corn hybrids co-inoculated with A. brasilense and A. cylindrica. The experiment was conducted in greenhouse conditions using the completely randomized design in a factorial scheme 2 × 4 with two hybrids, Balu 184 and Balu 280 Pro, and four inoculation treatments: no inoculation, inoculation of A. cylindrica, inoculation of A. brasilense, and the co-inoculation of A. cylindrica + A. brasilense. Co-inoculation resulted in higher root growth and dry mass of the aerial part in both hybrids; however, the hybrid Balu 280 Pro presented higher responsivity. The co-inoculation of A. brasilense and A. cylindrica increased initial corn growth without altering the photosynthetic pigment levels.


Revista CERES ◽  
2018 ◽  
Vol 65 (5) ◽  
pp. 407-414 ◽  
Author(s):  
Rodrigo Luiz Ludwig ◽  
Thomas Newton Martin ◽  
Jessica Deolinda Leivas Stecca ◽  
Vinícius dos Santos Cunha ◽  
Ubirajara Russi Nunes ◽  
...  

ABSTRACT The agronomic efficiency of the nitrogen-fixing inoculants or growth promoters depends on the growing conditions of the crops. One of the factors that may influence this response is the interaction between the bacteria present in the inoculant with the chemical treatment applied to the seeds. The objective of this study was to evaluate the influence of inoculation of wheat seeds with Azospirillum brasilense and its interaction with seed treatment on germination, vigor and initial growth of wheat plants of four cultivars. So, an experiment was carried out at the Federal University of Santa Maria, Santa Maria-RS. The experimental design was a completely randomized in a three-factorial (4x4x2), represented by the combinations between cultivar (FUNDACEP Bravo, OR/TBIO Quartzo, TBIO Itaipu e BRS 331), chemical treatment (1: Insecticide + Fungicide; 2: Insecticide; 3:Fungicide; 4: Witness without treatment) and Azospirillum brasilense inoculation (with and without inoculant). The determinations which were carried out were the following: emergence in beds, first germination test counting (vigor), germination, seedling length (root, aerial part and total), dry mass (root and aerial part). The responses to inoculants with Azospirillum brasilense are closely related to the cultivars used in the study. The treatment of seeds with fungicide and insecticide presented a phytotoxic effect, damaging the initial growth of the seedlings.


FLORESTA ◽  
2021 ◽  
Vol 51 (4) ◽  
pp. 855
Author(s):  
Francival Cardoso Felix ◽  
Daniele Cristina Pereira de Matos ◽  
Leandro Porto Latoh ◽  
Renata De Almeida Maggioni ◽  
Antônio Carlos Nogueira

Inoculation of rhizobacteria in seeds and plants has been documented as a stimulant and promoter of germination and growth of agricultural crops, but there is little information on native forest species. Therefore, the present study aims to evaluate the potential of Azospirillum brasilense inoculation in the germination of seeds and initial growth of seedlings of native forest species. The following experiments were carried out: (I) germination of seeds in germination chamber, and (II) initial growth of seedlings in nursery, both with four forest species (Cassia leptophylla, Lafoensia pacari, Mimosa flocculosa and Schinus terebinthifolius). For the four species, in both experiments, two treatments were used: inoculation of seeds with concentrated solution of A. brasilense and no inoculation of seeds (control). Physiological aspects of seed germination and seedling vigor were evaluated in the first experiment (germination speed, and length and dry weight of seedlings), and seedling growth was evaluated in the second experiment (height, collar diameter and dry weight of seedlings). Inoculation of A. brasilense in seeds of C. leptophylla, L. pacari, M. flocculosa and S. terebinthifolius did not promote beneficial or promising results for seed germination and initial growth of seedlings, so its use to produce seedlings of these species is not recommended.


2021 ◽  
Vol 8 (1) ◽  
pp. e5360
Author(s):  
Gabriel Monteiro Aguiar Pereira ◽  
Renato De Carvalho Menezes ◽  
Marcos Gomes da Cunha ◽  
Renato Carrer Filho

Biological formulations used as inoculants are increasingly present in grasses, especially in corn. Positive results in promoting plant growth, with different associations with diazotrophic bacteria, show these capacities of interaction to act as plant growth regulators, making it a promising alternative with a low environmental impact. Thus, this study aimed to evaluate the efficiency of inoculation and co-inoculation of nodulating and non-nodulating diazotrophic bacteria as promoters of initial growth in corn plants. Therefore, the bacteria Azospirillum brasilense (UFG21 strain), Bradyrhizobium japonicum (commercial product), and their co-inoculation were used in seed treatment. The evaluations consisted of growth measuring of the root system and shoot and evaluating leaf chlorophyll concentration. The results showed a beneficial interaction between the bacteria and the corn plant, with an increase in the root system and chlorophyll content, compared to the control treatment.


Author(s):  
Agner de Freitas Andrade ◽  
Tiago Zoz ◽  
André Zoz ◽  
Carlos Eduardo da Silva Oliveira ◽  
Travis Wilson Witt

ABSTRACT The Azospirillum brasilense inoculation is usually carried out via seed. However, this bacterium presents passive penetration in the plant, what makes it possible to inoculate the crops using other methods. This study aimed to evaluate the influence of A. brasilense inoculation methods on the emergence and initial growth of corn and sorghum, under greenhouse conditions. A randomized block design, with four replications, in a 4 x 2 factorial scheme, was used. The first factor consisted of a control treatment (without inoculation) and three inoculation methods: via seed, via sowing furrow and via leaf spray. The second factor was composed by two hybrids of corn and two of sorghum. The application of A. brasilense to the seed can reduce the emergence percentage and the emergence speed index of corn seedlings, depending on the genotype, but its application does not influence the initial growth of the plants. The A. brasilense inoculation via seed and via sowing furrow can reduce the emergence and increase the mean emergence time of sorghum seedlings, depending on the genotype. The A. brasilense inoculation via seed results in an increase in the dry mass of the root system of sorghum plants.


Author(s):  
Yoshichika Bando ◽  
Takahito Terashima ◽  
Kenji Iijima ◽  
Kazunuki Yamamoto ◽  
Kazuto Hirata ◽  
...  

The high quality thin films of high-Tc superconducting oxide are necessary for elucidating the superconducting mechanism and for device application. The recent trend in the preparation of high-Tc films has been toward “in-situ” growth of the superconducting phase at relatively low temperatures. The purpose of “in-situ” growth is to attain surface smoothness suitable for fabricating film devices but also to obtain high quality film. We present the investigation on the initial growth manner of YBCO by in-situ reflective high energy electron diffraction (RHEED) technique and on the structural and superconducting properties of the resulting ultrathin films below 100Å. The epitaxial films have been grown on (100) plane of MgO and SrTiO, heated below 650°C by activated reactive evaporation. The in-situ RHEED observation and the intensity measurement was carried out during deposition of YBCO on the substrate at 650°C. The deposition rate was 0.8Å/s. Fig. 1 shows the RHEED patterns at every stage of deposition of YBCO on MgO(100). All the patterns exhibit the sharp streaks, indicating that the film surface is atomically smooth and the growth manner is layer-by-layer.


Author(s):  
Xianghong Tong ◽  
Oliver Pohland ◽  
J. Murray Gibson

The nucleation and initial stage of Pd2Si crystals on Si(111) surface is studied in situ using an Ultra-High Vacuum (UHV) Transmission Electron Microscope (TEM). A modified JEOL 200CX TEM is used for the study. The Si(111) sample is prepared by chemical thinning and is cleaned inside the UHV chamber with base pressure of 1x10−9 τ. A Pd film of 20 Å thick is deposited on to the Si(111) sample in situ using a built-in mini evaporator. This room temperature deposited Pd film is thermally annealed subsequently to form Pd2Si crystals. Surface sensitive dark field imaging is used for the study to reveal the effect of surface and interface steps.The initial growth of the Pd2Si has three stages: nucleation, growth of the nuclei and coalescence of the nuclei. Our experiments shows that the nucleation of the Pd2Si crystal occurs randomly and almost instantaneously on the terraces upon thermal annealing or electron irradiation.


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