scholarly journals Virus titre determines the efficiency of Pentalonia nigronervosa (Aphididae: Hemiptera) to transmit banana bunchy top virus

VirusDisease ◽  
2018 ◽  
Vol 29 (4) ◽  
pp. 499-505
Author(s):  
Ravikumar Manohar Jebakumar ◽  
Velusamy Balasubramanian ◽  
Ramasamy Selvarajan
Keyword(s):  
Virus Titre ◽  
Banana Bunchy Top Virus ◽  
Pentalonia Nigronervosa

Banana Tree Infected with Banana Bunchy Top Virus Attracts Pentalonia nigronervosa Aphids Through Increased Volatile Organic Compounds Emission

2021 ◽  
Vol 47 (8-9) ◽  
pp. 755-767
Author(s):  
Ignace Safari Murhububa ◽  
Kévin Tougeron ◽  
Claude Bragard ◽  
Marie-Laure Fauconnier ◽  
Espoir Bisimwa Basengere ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Banana Bunchy Top Virus ◽  
Banana Tree ◽  
Volatile Organic ◽  
Pentalonia Nigronervosa

scholarly journals RNA-seq data of banana bunchy top virus (BBTV) viruliferous and non-viruliferous banana aphid (Pentalonia nigronervosa)

Data in Brief ◽  
2020 ◽  
Vol 28 ◽  
pp. 104860 ◽  
Author(s):  
Siti Subandiyah ◽  
Ruth Feti Rahayuniati ◽  
Sedyo Hartono ◽  
Susamto Somowiyarjo ◽  
Afiahayati ◽  
...  
Keyword(s):  
Rna Seq ◽  
Banana Bunchy Top Virus ◽  
Pentalonia Nigronervosa

scholarly journals PENGARUH WAKTU INFEKSI VIRUS KERDIL PISANG TERHADAP KERENTANAN TIGA KULTIVAR

2005 ◽  
Vol 5 (1) ◽  
pp. 42-49
Author(s):  
Dewi Widyastuti ◽  
Sri Hendrastuti Hidayat
Keyword(s):  
Disease Incidence ◽  
Virus Transmission ◽  
Leaf Size ◽  
Growth Stages ◽  
Virus Concentration ◽  
Adaptation Period ◽  
Banana Bunchy Top Virus ◽  
Time Of Infection ◽  
Pentalonia Nigronervosa

Effects of time of infection of banana bunchy top virus on susceptibility of three banana cultivars. Banana Bunchy Top, caused by Banana Bunchy Top Virus (BBTV), is one of the most important banana diseases in Indonesia. Approach to reduce disease incidence involves prevention of early infection especially on susceptible cultivars. This study was conducted to evaluate the response of three banana cultivars, Ambon Kuning, Tanduk, and Kepok, to different time of infection of BBTV i.e., one week and three week after adaptation period, and one week during adaptation period. Banana plants used in the study were prepared through in vitro propagation (tissue culture) and virus transmission was done using aphid vector, Pentalonia nigronervosa.  In addition to observation on symptom expression, inhibition of plant height, and reduction of leaf size, conformation of virus infection was done through indirect ELISA. Virus concentration on different part of the plant, young leaf, stem, and root, tends to decrease over the time due to the ability of BBTV to move from cell to cell before replication takes place. It is evidenced that BBTV was able to infect banana in all growth stages although the younger plant is more susceptible to BBTV. Although concentration of the virus in the tested plant is considered high, symptoms expression of BBTV infection can be differentiated from moderate to very severe. Response of banana plants to infection of BBTV can be grouped into susceptible (Ambon Kuning), moderate tolerant (Tanduk), and tolerant (Kepok).  

scholarly journals Response of banana germplasms to banana bunchy top virus

2021 ◽  
Vol 948 (1) ◽  
pp. 012022
Author(s):  
D Arubi ◽  
Giyanto ◽  
D Dinarty ◽  
A Sutanto ◽  
S H Hidayat
Keyword(s):  
Yield Loss ◽  
Disease Incidence ◽  
Significant Inhibition ◽  
Feeding Period ◽  
Sources Of Resistance ◽  
Banana Bunchy Top Virus ◽  
Wild Accessions ◽  
Pentalonia Nigronervosa ◽  
Test Plants ◽  
Observation Period

Abstract Banana bunchy top virus (BBTV) is one of the important viruses causing disease in bananas and its infection has the potential to cause yield loss. This study was conducted to evaluate the response of several commercial cultivars (Cavendish, Bebek, Goroho, Tanduk, and Barangan Merah) and wild accessions (Klutuk NTT, Halabanensis, SPn 001, LNT 001, and Microcarpa) of banana to BBTV infection. Transmission of BBTV was carried out through banana aphid Pentalonia nigronervosa, using 20 adult aphids per plant with an acquisition feeding period of 24 hours on BBTV-infected plants and an inoculation feeding period on healthy test plants for 48 hours. Observation on plant growth and disease intensity was conducted for 8 weeks after inoculation. At the end of the observation period, only 5 cultivars, i.e. Cavendish, Bebek, Goroho, Barangan Merah, and Halabanensis showed typical symptoms of BBTV with disease incidence reached 80%, 60%, 20%, 20%, and 20% respectively. Significant inhibition of plant height and leaves width occurred in Cavendish, Bebek, and Goroho i.e. 44.60%, 36.31%; 12.62%, 41.08%; and 25%, 10.13%, respectively. This paper discusses the need for banana germplasm exploration to find sources of resistance to BBTV.

scholarly journals Effect of Banana Bunchy Top Virus on the Heat Shock Protein Genes of Pentalonia nigronervosa during Temperature Susceptibility and Its Effect on Virus Transmission

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1866
Author(s):  
Swati Chakraborty ◽  
Mritunjoy Barman ◽  
Snigdha Samanta ◽  
Moupiya Roy ◽  
Jayanta Tarafdar
Keyword(s):  
Developmental Biology ◽  
Heat Shock ◽  
Temperature Stress ◽  
Virus Titer ◽  
Plant Viruses ◽  
Effect Of Temperature ◽  
Banana Bunchy Top Virus ◽  
Differential Expression Pattern ◽  
Pentalonia Nigronervosa ◽  
Temperature Susceptibility

Acquisition of plant viruses is known to exert various effects on vectors’ developmental biology. Pentalonia nigronervosa is the only known vector of banana bunchy top virus (BBTV), which is an economically detrimental virus infecting banana cultivars all over the world. In the present study, the developmental biology of viruliferous (Vr) and non-viruliferous (NVr) aphids was compared, with a marked reduction noted in the lifespan of aphids upon acquisition of BBTV. Among all the environmental parameters temperature is an important determinant of an insect’s abundance and geographical distribution. Temperature susceptibility of P. nigronervosa was scrutinized by comparing the mortality percentage and differential expression pattern of three heat shock proteins (Hsps; Hsp40, Hsp70, and Hsp90) at the mRNA level between NVr and Vr aphids. After exposure to different temperature stress (5 °C, 15 °C, 38 °C and 25 °C as control) highest mortality of Vr aphids were recorded at 5 °C. Analysis of expression levels of Hsp genes using qPCR showed that both cold and heat shock treatment stimulated higher expression of the three Hsps at various rates in Vr than NVr aphids.. Finally, the effect of temperature stress on the BBTV titer level and their transmission by P.nigronervosa was determined by absolute quantification. The transmission efficiency along with the virus titer was found to be the lowest at 15 °C compared to 38 °C. Overall, our results provide a novel insight into the intricate interaction between aphid fitness and thermal stress concerning the acquisition and transmission of BBTV, which could be a roadmap for the future epidemiological control system.

scholarly journals Transgenic Expression of dsRNA Targeting the Pentalonia nigronervosa acetylcholinesterase Gene in Banana and Plantain Reduces Aphid Populations

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 613
Author(s):  
Temitope Jekayinoluwa ◽  
Jaindra Nath Tripathi ◽  
Benjamin Dugdale ◽  
George Obiero ◽  
Edward Muge ◽  
...  
Keyword(s):  
Insect Vector ◽  
Banana Bunchy Top Virus ◽  
Aphid Infestation ◽  
Transgenic Expression ◽  
Aphid Feeding ◽  
Essential Enzyme ◽  
Pentalonia Nigronervosa ◽  
Acetylcholinesterase Gene ◽  
Aphid Populations

The banana aphid, Pentalonia nigronervosa, is the sole insect vector of banana bunchy top virus (BBTV), the causal agent of banana bunchy top disease. The aphid acquires and transmits BBTV while feeding on infected banana plants. RNA interference (RNAi) enables the generation of pest and disease-resistant crops; however, its effectiveness relies on the identification of pivotal gene sequences to target and silence. Acetylcholinesterase (AChE) is an essential enzyme responsible for the hydrolytic metabolism of the neurotransmitter acetylcholine in animals. In this study, the AChE gene of the banana aphid was targeted for silencing by RNAi through transgenic expression of AChE dsRNA in banana and plantain plants. The efficacy of dsRNA was first assessed using an artificial feeding assay. In vitro aphid feeding on a diet containing 7.5% sucrose, and sulfate complexes of trace metals supported aphid growth and reproduction. When AChE dsRNA was included in the diet, a dose of 500 ng/μL was lethal to the aphids. Transgenic banana cv. Cavendish Williams and plantain cvs. Gonja Manjaya and Orishele expressing AChE dsRNA were regenerated and assessed for transgene integration and copy number. When aphids were maintained on elite transgenic events, there was a 67.8%, 46.7%, and 75.6% reduction in aphid populations growing on Cavendish Williams, Gonja Manjaya, and Orishele cultivars, respectively, compared to those raised on nontransgenic control plants. These results suggest that RNAi targeting an essential aphid gene could be a useful means of reducing both aphid infestation and potentially the spread of the disease they transmit.

scholarly journals Effect of Temperature, Vector Life Stage, and Plant Access Period on Transmission of Banana bunchy top virus to Banana

2008 ◽  
Vol 98 (6) ◽  
pp. 743-748 ◽  
Author(s):  
M. D. Anhalt ◽  
R. P. P. Almeida
Keyword(s):  
Life Stage ◽  
Plant Viruses ◽  
Transmission Efficiency ◽  
Effect Of Temperature ◽  
Access Time ◽  
Banana Bunchy Top Virus ◽  
Access Period ◽  
Pentalonia Nigronervosa ◽  
The Relationship ◽  
Transmission Biology

The study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. We characterized the transmission of a nanovirus, Banana bunchy top virus (BBTV), by its aphid vector Pentalonia nigronervosa Coquerel (Hemiptera, Aphididae) with respect to temperature, vector life stage, and plant access time. Adult aphids transmitted BBTV more efficiently than third instar nymphs at all temperatures tested. Adult aphids transmitted the virus more efficiently at 25 and 30°C than at 20°C, but temperature had no impact on transmission efficiency by nymphs. By decoupling the relationship between temperature and aphid BBTV acquisition or inoculation, we determined that temperature affected inoculation events more strongly than acquisition. Longer plant access periods increased viral acquisition and inoculation efficiencies in a range of 60 min to 24 h. Both BBTV acquisition and inoculation efficiencies peaked after 18 h of plant access period. We also show that BBTV transmission by P. nigronervosa requires a latent period. Our results demonstrate that vector transmission of BBTV is affected by temperature, vector life stage, and plant access period.

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