Antagonistic activity and mechanism of a novel Bacillus amyloliquefaciens MB40 strain against fire blight

2020 ◽  
Vol 102 (3) ◽  
pp. 825-833
Author(s):  
Olga Shemshura ◽  
Mereke Alimzhanova ◽  
Elvira Ismailova ◽  
Assel Molzhigitova ◽  
Saule Daugaliyeva ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Fire Blight ◽  
Antagonistic Activity

scholarly journals ANTAGONISTIC ACTIVITY OF PAENIBACILLUS POLYMYXA, BACILLUS AMYLOLIQUEFACIENS AND THEIR LASER STIMULATION AGAINST PHYTOPATHOGENS

2021 ◽  
Vol 3 (27) ◽  
pp. 135-134
Author(s):  
M.V. Maslova ◽  
◽  
E.V. Grosheva ◽  
A.V. Budagovsky ◽  
O.N. Budagovskaya ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Pseudomonas Syringae ◽  
Bacterial Strain ◽  
Bacillus Amyloliquefaciens ◽  
Paenibacillus Polymyxa ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Light Irradiation ◽  
Bacterial Cells ◽  
Coherent Light

Nowadays, thanks to organic farming development, particular attention is paid to the biological methods of plants protection, especially from diseases. Therefore, new biological preparations creation and studying their effectiveness are promising directions. The aim of the research was to study the antagonistic activity of the bacteria Paenibacillus polymyxa and Bacillus amyloliquefaciens against some phytopathogens (Pseudomonas syringae van Hall, Fusarium oxysporum Schltdl, Alternaria alternata (Fr.) Keissl.), as well as their reaction to laser irradiation. The research was carried out in the Research Problem Laboratory “Biphotonika” of the Michurinsk State Agrarian University and the Department of Agricultural Microbiology of the Research Institute of Agriculture of Crimea in 2016–2021. The antagonistic activity of the bacteria was evaluated by the double culture method. The effectiveness of irradiation (as a result of their treatment with coherent light) was determined by the change in the number of bacterial cells in the suspension. The study of the activity of bacteria-antagonists against pathogens showed that the suppression of P. syringae, F. oxysporum, A. alternata growth in the double culture with P. polymyxa compared to the control variants was 32.5 %; 4.0 % and 77.9 %, respectively. B. amyloliquefaciens suppressed the growth of P. syringae by 25.9 %, F. oxysporum – by 49.0 %; A. alternate – by 61.1 %. An increase in the number of cells in suspensions of P. polymyxa and B. amyloliquefaciens after coherent light irradiation by 26.6 % and 36.7 %, respectively, was also found. Thus, to control P. syringae, bacterial strain of P. polymyxa P was more effective. The bacterial strain B. amyloliquefaciens 01-1 showed antagonistic activity against F. oxysporum. Both studied microorganisms were effective against A. alternata. To increase the activity of cell division of antagonist strains of phytopathogens, it is advisable to use coherent light irradiation. In future, it is advisable to test the level of antagonistic activity of the bacteria P. polymyxa and B. amyloliquefaciens after laser irradiation.

scholarly journals Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

2012 ◽  
Vol 79 (3) ◽  
pp. 808-815 ◽  
Author(s):  
Zhihui Xu ◽  
Jiahui Shao ◽  
Bing Li ◽  
Xin Yan ◽  
Qirong Shen ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bacillus Amyloliquefaciens ◽  
Root Colonization ◽  
Antagonistic Activity ◽  
Vital Role ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Bacillomycin D

ABSTRACTBacillus amyloliquefaciensstrains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. In this study, we report that one of the lipopeptide compounds (bacillomycin D) produced by the rhizosphere strainBacillus amyloliquefaciensSQR9 not only plays a vital role in the antagonistic activity againstFusarium oxysporumbut also affects the expression of the genes involved in biofilm formation. When the bacillomycin D and fengycin synthesis pathways were individually disrupted, mutant SQR9M1, which was deficient in the production of bacillomycin D, only showed minor antagonistic activity againstF. oxysporum, but another mutant, SQR9M2, which was deficient in production of fengycin, showed antagonistic activity equivalent to that of the wild-type strain ofB. amyloliquefaciensSQR9. The results fromin vitro, rootin situ, and quantitative reverse transcription-PCR studies demonstrated that bacillomycin D contributes to the establishment of biofilms. Interestingly, the addition of bacillomycin D could significantly increase the expression levels ofkinCgene, but KinC activation is not triggered by leaking of potassium. These findings suggest that bacillomycin D contributes not only to biocontrol activity but also to biofilm formation in strainB. amyloliquefaciensSQR9.

scholarly journals Bacterial species recognized for the first time for its biocontrol activity against fire blight (Erwinia amylovora)

2019 ◽  
Vol 156 (1) ◽  
pp. 257-272 ◽  
Author(s):  
Artur Mikiciński ◽  
Joanna Puławska ◽  
Assel Molzhigitova ◽  
Piotr Sobiczewski
Keyword(s):  
Fire Blight ◽  
Dna Analysis ◽  
Erwinia Amylovora ◽  
Bacterial Species ◽  
Antagonistic Activity ◽  
Protective Capacity ◽  
Biocontrol Activity ◽  
Enterobacter Ludwigii ◽  
First Time ◽  
Do So

AbstractThis study included eight bacterial isolates originating from the apple phyllosphere or soil environment that were previously selected using the pear fruitlet test (Mikiciński 2017). Identification of these isolates based on phenotypic assays and DNA analysis showed that five of them belonged to species for which an antagonistic activity against Erwinia amylovora and the protective capacity of apple and pear against fire blight were not previously demonstrated. These were L16 identified as Pseudomonas vancouverensis, 3 M as Pseudomonas chlororaphis subsp. aureofaciens, 35 M – Pseudomonas congelans, 43 M – Enterobacter ludwigii, and 59 M – Pseudomonas protegens. Investigation of the biotic relationships between the tested strains and E. amylovora showed that 3 M, 35 M and 59 M inhibited the growth of the pathogen on five out of six media used (NAS, KB, LB, R2A, NAG), but 43 M did not do so on any of these media. Strain L16 did not inhibit the growth of the pathogen on LB or R2A medium. In contrast, all strains grown on medium 925 stimulated the growth of the pathogen, which showed no growth without co-cultivation with these strains. The experiments on apple trees and detached apple branches showed the ability of the tested bacteria to protect flowers at medium to high levels, depending on the experiment (55–93%). In some cases, this protection was even higher than that of the copper product used for comparison. In studies assessing the bacterial ability to protect shoots of M.26, the highest efficacy was observed for strains 35 M (96%) and 43 M (93%) but on ‘Gala Must’ all tested strains showed 100% of efficacy.

scholarly journals Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease

2009 ◽  
Vol 140 (1-2) ◽  
pp. 38-44 ◽  
Author(s):  
X.H. Chen ◽  
R. Scholz ◽  
M. Borriss ◽  
H. Junge ◽  
G. Mögel ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Fire Blight ◽  
Blight Disease

scholarly journals <i>In Vitro</i> Antagonistic Activity of <i>Trichoderma harzianum</i> and <i>Bacillus amyloliquefaciens</i> against <i>Colletotrichum acutatum</i>

2020 ◽  
Vol 10 (03) ◽  
pp. 82-94
Author(s):  
Rababe Es-Soufi ◽  
Houda Tahiri ◽  
Latifa Azaroual ◽  
Aïcha El Oualkadi ◽  
Patrick Martin ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Bacillus Amyloliquefaciens ◽  
Antagonistic Activity ◽  
Colletotrichum Acutatum

scholarly journals INFLUENCE OF GENUS BACTERIA BACILLUS ON QUALITY OF Lactuca sativa L.

2019 ◽  
pp. 49-52
Author(s):  
O. V. Doroshchuk ◽  
J. N. Kalatskaja ◽  
N. A. Laman ◽  
V. V. Minkova ◽  
M. N. Mandrik-Litvinkovich
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Amyloliquefaciens ◽  
Antagonistic Activity ◽  
Matter Content ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soil Conditions ◽  
Dry Matter Content ◽  
Wide Range

Primal problem of vegetable growing is constant supply of the population with all types of vegetables, including green cultures. Green cultures are vegetables that have high nutritional value and precocity. However they often are infected by phytopathogenic microorganisms already at initial stages of ontogenesis at cultivation in closed soil conditions. It leads to emergence of disjointed shoots, deterioration of growth and development of plants and loss of quality. Now in the Republic of Belarus a number of biological substances on the basis of bacteria Bacillus was developed. They are used against diseases of plants of mushroom and bacterial etiology. However there is not information about influence of bacteria on quality of products of green cultures. The aim of the work was studying of influence of two strains of bacteria Bacillus that were introduced in peat substrate on efficiency and quality of lettuce. Two strains of bacteria Bacillus were used in the work. They were selected from the soil. The strains are Bacillus subtilis M9/6 and Bacillus amyloliquefaciens 23TM that have high antagonistic activity to a wide range of phytopathogens. Cultivation of plants carried out in containers of 250 ml under light installations with illuminating intensity 13-15 thousand luxury and lasting irradiating of 14 hours before technical ripeness of lettuce. It was established that the application of strain Bacillus subtilis M9/6 (in concentration 106 cells/ml, 10 ml/l of substrate) and the strain Bacillus amyloliquefaciens 23TM (5 ml/l of substrate) in substrate before sowing increased nutrition value of lettuce. Dry matter content, water-soluble carbohydrates (mono - and disaccharides) content and vitamin C content increased. The bacterial strain B. amyloliquefaciens 23TM also promoted accumulation of vitamin B2. The content of nitrates in lettuce leaves decreased on 50,3% and 39,1%, respectively. It was shown that the application of bacteria in substrate before sowing of crop has a greater influence on quality of lettuce, than watering of shoots.

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