Oral Microflora, in Cases with Gingival Hypertrophy Caused by Fixed Orthodontic Appliances

Changes in the contents of the oral flora, in gingival hypertrophy caused by fixed orthodontic appliances (Preprint)

10.2196/preprints.24263 ◽

2020 ◽

Author(s):

Ilma Robo ◽

Saimir Heta ◽

Enxhi Gjumsi ◽

Vera Ostreni

Keyword(s):

Oral Cavity ◽

Topical Treatment ◽

Orthodontic Treatment ◽

Orthodontic Appliances ◽

Laboratory Examination ◽

Oral Flora ◽

Streptococcus Anginosus ◽

Fixed Orthodontic Appliances ◽

Gingival Hypertrophy

BACKGROUND The placement of orthodontic apparatus in the oral cavity, according to the literature, should influence the alteration of oral flora, especially the subgingival one. The purpose of the study is to evaluate the subgingival flora of patients with fixed orthodontic appliances, regardless of placement time. OBJECTIVE The purpose of the study is to evaluate the subgingival flora of patients with fixed orthodontic appliances, regardless of placement time. METHODS In 3 cases of patients with fixed orthodontic appliances, a bacterial sample of the gingival sulcus was taken for laboratory examination. Patients were clinically evaluated for the presence or tendency, of having gingival hypertrophy. RESULTS Results from the 3 cases included in the study, 1 of them came up with Streptococcus Anginosus positive, Doxycilin-sensitive. The tendency for gingival hypertrophy was maximal 3% to 1.5% respectively in each patient. In the patient with different oral flora, daily topical treatment with tetracycline, placed in the gingival sulcus, was applied. CONCLUSIONS Alteration of the oral flora with the placement of fixed orthodontic appliances is not a fully verifiable fact, as it indicates the patient's follow-up, at the time of placement of the apparatus and until removal after orthodontic treatment, depending on the 2-3-year period of treatment. The tendency for gingival hypertrophy is apparently high, versus the presence of fixed orthodontic apparatus.

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Influence of Propolis on Hygiene, Gingival Condition, and Oral Microflora in Patients with Cleft Lip and Palate Treated with Fixed Orthodontic Appliances

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/183915 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Agnieszka Machorowska-Pieniążek ◽

Tadeusz Morawiec ◽

Anna Mertas ◽

Marta Tanasiewicz ◽

Arkadiusz Dziedzic ◽

...

Keyword(s):

Cleft Lip ◽

Cleft Lip And Palate ◽

Ethanol Extract ◽

Control Group ◽

Orthodontic Appliances ◽

Fixed Appliance ◽

Oral Microflora ◽

Fixed Orthodontic Appliances ◽

Bilateral Cleft Lip ◽

Bacterial Plaque

The aim of this study was to evaluate the influence of 3% ethanol extract of propolis (EEP) on hygiene, gingival and microbiological status of oral cavity in patients with cleft lip and palate treated with fixed orthodontic appliances. The study included forty-one nonsyndromic complete unilateral of bilateral cleft lip and palate subjects with fixed appliance on at least 10 teeth. Twenty-one subjects were instructed to brush their teeth three times a day using toothpaste with propolis. Control group included twenty subjects who were asked to brush their teeth three times a day using a toothpaste without propolis. API, OPI, GI, and supragingival bacterial plaque were taken from each subject twice: baseline and after using the toothpaste for 35 days. The final examinations showed statistically significant decrease in OPI, GI, and the percentage of theActinomycesspp. andCapnocytophagaspp. compared with baseline in propolis group subjects. The improvement in oral health in these patients confirms antibacterial, anti-inflammatory, and regenerative properties of propolis.

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MICROBIOLOGICAL TESTS AS INDICATORS OF CARIES RISK

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії ◽

10.31718/2077-1096.20.3.51 ◽

2020 ◽

Vol 20 (3) ◽

pp. 51-54

Author(s):

A.V. Zaitsev ◽

O.N. Boychenko ◽

N.V. Kotelevskaya ◽

A.K. Nikolishin

Keyword(s):

Dental Caries ◽

Moderate Intensity ◽

Orthodontic Appliances ◽

Application Range ◽

Oral Microflora ◽

Quantitative Content ◽

Fixed Orthodontic Appliances ◽

Rapid Tests ◽

Carious Process ◽

Carious Teeth

Fixed orthodontic appliances are widely used in the correction of dentoalveolar anomalies. Examinations prior the beginning of orthodontic treatment revealed numerous cases of dental caries of moderate intensity; moreover, caries prevalence increases with age that is the number of carious teeth goes up, while the number of filled teeth and the level of oral hygiene become lower. A voluminous body of reports indicates that children and adolescents wearing orthodontic appliances have a significantly higher incidence of caries than patients who do not wear them. Plaque microflora is one of the main risk factors for the development of caries. According to the researchers, caries is initiated by streptococci, lactobacilli, actinomycetes, and Candida fungi. To assess the content of cariogenic microorganisms (Streptococcus mutans and Lactobacillus), there are special microbiological tests available. Kits for rapid tests are manufactured in different countries around the world. In 2013, the researchers of the Department of Therapeutic Dentistry and the Department of Microbiology, Virology and Immunology, Ukrainian Medical Stomatological Academy investigated the microbiocenosis in the oral cavity in young people aged 19-25 years with different intensity of the carious process. The percentage and quantitative content of microflora was studied. The results of the study showed that an increase in the intensity of dental caries is accompanied by changes in the balance in the composition of the oral microflora. This study elaborated the indicators for identifying lactobacilli, streptococci (including haemolytic streptococci, which include S. mutans), fungi of the genus Candida and actinomycetes. But the results obtained did not match the application range of the above mentioned tests. It means the tests reflecting the number of representatives of the studied microbiocenosis in order to determine their activity, are not sufficiently objective. However, rapid tests assessing the indices of cariogenic microflora are in demand in dental practice that allows us to recommend their application in orthodontics. Further study of microbiocenoses should be carried out to determine their functional properties.

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The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients

Scientific Reports ◽

10.1038/s41598-020-78971-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Isamu Kado ◽

Junzo Hisatsune ◽

Keiko Tsuruda ◽

Kotaro Tanimoto ◽

Motoyuki Sugai

Keyword(s):

Relative Abundance ◽

Japanese Patients ◽

Oral Microbiome ◽

Orthodontic Appliances ◽

Periodontal Pathogens ◽

Supragingival Plaque ◽

Fixed Orthodontic Appliances ◽

Microbial Dna ◽

The Impact ◽

Level Analysis

AbstractFixed orthodontic appliances are common and effective tools to treat malocclusion. Adverse effects of these appliances, such as dental caries and periodontitis, may be associated with alteration of the microbiome. This study investigated the impact of these appliances on the dynamics of the oral microbiome. Seventy-one patients were selected. Supragingival plaque samples were collected before placement (T0) and six months after placement (T1). Saliva samples were collected at T0 and T1, and then when appliance removal (T2). Microbial DNA was analyzed by 16S rRNA meta-sequencing. The diversity analysis indicated dynamic changes in the structure of the oral microbiome. Taxonomic analysis at phylum level showed a significant increase in Bacteroidetes and Saccharibacteria (formally TM7) and decrease in Proteobacteria and Actinobacteria over time, in both plaque and saliva. Genus level analysis of relative abundance indicated a significant increase in anaerobic and facultative anaerobes in both plaque and saliva. Fixed orthodontic appliances induced measurable changes in the oral microbiome. This was characterized by an increase in relative abundance of obligate anaerobes, including periodontal pathogens. It can be concluded that this dysbiosis induced by fixed orthodontic appliances is likely to represent a transitional stage in the shift in microbiome from healthy to periodontitis.

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A single-centre investigator-blinded randomised parallel group clinical trial to investigate the effect of probiotic strainsStreptococcus salivariusM18 andLactobacillus acidophiluson gingival health of paediatric patients undergoing treatment with fixed orthodontic appliances: study protocol

BMJ Open ◽

10.1136/bmjopen-2019-030638 ◽

2019 ◽

Vol 9 (9) ◽

pp. e030638 ◽

Cited By ~ 2

Author(s):

Eleftherios G Kaklamanos ◽

Rania Nassar ◽

Sotirios Kalfas ◽

Manal Al Halabi ◽

Mawlood Kowash ◽

...

Keyword(s):

Treatment Group ◽

Oral Hygiene ◽

Oral Microbiome ◽

Control Group ◽

Orthodontic Appliances ◽

Single Centre ◽

Paediatric Patients ◽

Gingival Health ◽

Parallel Group ◽

Fixed Orthodontic Appliances

BackgroundThere is limited data on the beneficial effects of probiotics on the gingival health of patients undergoing treatment with fixed orthodontic appliances. This study aims to compare the effect of probiotic tablets combined with regular oral hygiene versus regular oral hygiene alone on gingival status in these patients. The effect of probiotic intake on plaque formation and salivary microbiome composition will be also assessed.Methods and analysisThis is a 3 month single-centre, single blind (clinical and laboratory examiners), parallel group randomised controlled two arm superiority trial. Fifty paediatric patients attending the Postgraduate Orthodontic Clinic at the Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai, United Arab Emirates, who meet the eligibility criteria will be recruited. Block randomisation with 1:1 allocation and concealment of allocation will be carried out. The treatment group will receive probiotic tablets containingStreptococcus salivariusM18 andLactobacillus acidophilustogether with regular oral hygiene versus the control group on regular oral hygiene alone. Clinical examination and collection of saliva for microbiome assay will be carried out at baseline and end of study. Self-reporting by patients will be used to document acceptability and adverse effects. Statistically significant decrease in gingival bleeding on probing in the treatment group will be classified as primary outcome of treatment success. Statistically significant reduction in Plaque Index, Gingival Index and shift in the composition of the oral microbiome in favour of beneficial bacteria are secondary outcomes indicative of efficacy of probiotic intake.Ethics and disseminationEthical approval for the study has been granted by the HBMCDM, MBRU, Institutional Review Board (Reference #: MBRU-IRB-2018–015). Study findings will be disseminated via publication in peer-reviewed journal.Trial registration numberISRCTN95085398

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