The Anti-oxidative Effects of Encapsulated Cysteamine During Mice In Vitro Matured Oocyte/Morula-Compact Stage Embryo Culture Model: a Comparison of High-Efficiency Nanocarriers for Hydrophilic Drug Delivery—a Pilot Study

Chemotherapy efficacy is often reduced by insufficient drug uptake in tumor cells. The combination of ultrasound and microbubbles (USMB) has been shown to improve drug delivery and to enhance the efficacy of several drugs in vitro and in vivo, through effects collectively known as sonopermeation. However, clinical translation of USMB therapy is hampered by the large variety of (non-clinical) US set-ups and US parameters that are used in these studies, which are not easily translated to clinical practice. In order to facilitate clinical translation, the aim of this study was to prove that USMB therapy using a clinical ultrasound system (Philips iU22) in combination with clinically approved microbubbles (SonoVue) leads to efficient in vitro sonopermeation. To this end, we measured the efficacy of USMB therapy for different US probes (S5-1, C5-1 and C9-4) and US parameters in FaDu cells. The US probe with the lowest central frequency (i.e. 1.6 MHz for S5-1) showed the highest USMB-induced intracellular uptake of the fluorescent dye SYTOX™ Green (SG). These SG uptake levels were comparable to or even higher than those obtained with a custom-built US system with optimized US parameters. Moreover, USMB therapy with both the clinical and the custom-built US system increased the cytotoxicity of the hydrophilic drug bleomycin. Our results demonstrate that a clinical US system can be used to perform USMB therapy as efficiently as a single-element transducer set-up with optimized US parameters. Therefore, future trials could be based on these clinical US systems, including validated US parameters, in order to accelerate successful translation of USMB therapy.

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An in-vitro study of ginsenoside Rb1-induced teratogenicity using a whole rat embryo culture model

Human Reproduction ◽

10.1093/humrep/deg401 ◽

2003 ◽

Vol 18 (10) ◽

pp. 2166-2168 ◽

Cited By ~ 44

Author(s):

L.Y. Chan

Keyword(s):

Embryo Culture ◽

In Vitro Study ◽

Vitro Study ◽

Ginsenoside Rb1 ◽

Rat Embryo ◽

Culture Model ◽

Rat Embryo Culture

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Novel Surface Modification of ZnO QDs for Paclitaxel-Targeted Drug Delivery for Lung Cancer Treatment

Dose-Response ◽

10.1177/1559325820926739 ◽

2020 ◽

Vol 18 (2) ◽

pp. 155932582092673

Author(s):

Chuan Xie ◽

Yan Zhan ◽

Peng Wang ◽

Bo Zhang ◽

Yukun Zhang

Keyword(s):

Drug Delivery ◽

Surface Modification ◽

Cancer Treatment ◽

Drug Delivery System ◽

Delivery System ◽

High Efficiency ◽

A549 Cells ◽

Cytotoxicity Studies

Adipic dihydrazide and heparin were attached to ZnO quantum dots surface, and the ZnO-adipic dihydrazide-heparin nanocomplex was used as a drug delivery system to deliver paclitaxel for chemotherapy. The surface modification and the loading of paclitaxel were confirmed by Fourier transform infrared spectrum, featured by characteristic peaks from functional groups of adipic dihydrazide, heparin, and paclitaxel. The impacts of pH on the drug release were investigated, and the cytotoxicity studies were conducted with A549 cells. The pharmacokinetic study was conducted with male Wistar rats. Both in vitro and in vivo study indicated that ZnO-adipic dihydrazide-heparin-paclitaxel nanocomplex could deliver paclitaxel in a more controllable way, and it has the potential to be a high-efficiency drug delivery system for cancer treatment.

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Review: Role of tubal environment in preimplantation embryogenesis: application to co-culture assays

Zygote ◽

10.1017/s0967199410000092 ◽

2010 ◽

Vol 19 (1) ◽

pp. 47-54 ◽

Cited By ~ 11

Author(s):

Pierre Guérin ◽

Yves Ménézo

Keyword(s):

Amino Acids ◽

Embryo Culture ◽

Metabolic Flux ◽

Cultured Cells ◽

Culture Media ◽

Culture Conditions ◽

Antioxidant Compounds ◽

Beneficial Effects ◽

Stage Embryo

SummaryThe culture of early preimplantation stage embryo is still delicate and the metabolic pathways of embryos are not completely understood. Embryo needs are evolutionary during the preimplantation development, consequently it is difficult to meet embryo needs in vitro. Culture conditions have to respect several physical and chemical equilibria: such as redox potential, pH, osmotic pressure, metabolic flux of energetic compounds, endogenous pools of amino acids and transcripts, etc. Embryo culture media are generally supplemented with amino acids, glucose, other energetic metabolites and antioxidant compounds, vitamin, and growth factors etc. Furthermore autocrine and paracrine regulation of embryo development probably exist. In fact embryo culture conditions have to be as non-toxic as possible. Various types of co-culture systems have been devised to overcome these problems. Complex interrelations exist between embryos and co-cultured cells. The beneficial effects of co-cultured cells may be due to continuous modifications of the culture medium, i.e. the elimination of toxic compounds and/or the supply of embryotrophic factors.

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Evaluation of Poly (Glycerol-Adipate) Nanoparticle Uptake in an In Vitro 3-D Brain Tumor Co-Culture Model

Experimental Biology and Medicine ◽

10.3181/0612-rm-301 ◽

2007 ◽

Vol 232 (8) ◽

pp. 1100-1108 ◽

Cited By ~ 25

Author(s):

W. Meng ◽

P. Kallinteri ◽

D. A. Walker ◽

T. L. Parker ◽

M. C. Garnett

Keyword(s):

Drug Delivery ◽

Brain Tumor ◽

Single Cells ◽

Brain Slices ◽

Brain Cells ◽

Normal Brain ◽

New Model ◽

Culture Model

Despite the inherent problems associated with in vivo animal models of tumor growth and metastases, many of the current in vitro brain tumor models also do not accurately mimic tumor-host brain interactions. Therefore, there is a need to develop such co-culture models to study tumor biology and, importantly, the efficacy of drug delivery systems targeting the brain. So far, few investigations of this nature have been published. In this paper we describe the development of a new model system and its application to drug delivery assessment. For our new model, a co-culture of DAOY cell brain tumor aggregates and organo-typic brain slices was developed. Initially, the DAOY aggregates attached to cerebellum slices and invaded as a unit. Single cells in the periphery of the aggregate detached from the DAOY aggregates and gradually replaced normal brain cells. This invasive behavior of DAOY cells toward organotypic cerebellum slices shows a similar pattern to that seen in vivo. After validation of the co-culture model using transmission electron microscopy, nanoparticle (NP) uptake was then evaluated. Confocal micrographs illustrated that DAOY cells in this co-culture model took up most of the NPs, but few NPs were distributed into brain cells. This finding corresponded with results of NP uptake in DAOY and brain aggregates reported elsewhere.

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Applications of ROS-Induced Zr-MOFs Platform in Multimodal Synergistic Therapy

Mini-Reviews in Medicinal Chemistry ◽

10.2174/1389557521666210105111850 ◽

2021 ◽

Vol 21 ◽

Author(s):

Qiongjie Ding ◽

Yiwei Liu ◽

Chuncheng Shi ◽

Jifei Xiao ◽

Wei Dai ◽

...

Keyword(s):

Drug Delivery ◽

High Efficiency ◽

Tumor Therapy ◽

Antitumor Effects ◽

Metal Organic ◽

Low Toxicity ◽

Therapeutic Mechanisms ◽

Biological Performance

Background: Metal-organic frameworks (MOFs) exhibited the adjustable aperture, high load capacities, tailorable structures, and excellent biocompatibilities that have used to be as drug delivery carries in cancer therapy. Until now, Zr-MOFs in particular combine optimal stability towards hydrolysis and postsynthetic modification with low toxicity, and are widely studied for its excellent biological performance. Introduction: This review comprises the exploration of Zr-MOFs as drug delivery devices (DDSs) with focus on various new methods, including chemotherapy (CT), photodynamic therapy (PDT), photothermal therapy (PTT), sonodynamic therapy(SDT), radiotherapy, immunotherapy, gene therapy and related combined therapies, which all generate reactive oxygen species (ROS) to achieve the high efficiency of tumor therapy. Conclusion: We described and summarized these pertinent examples of the therapeutic mechanisms and highlight the antitumor effects of their biological application both in vitro and in vivo. The perspectives on their future applications and analogous challenge of the Zr-MOFs materials are given.

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FERTILITAS DAN PERSENTASE EMBRIO KERBAU SAMPAI MORULA YANG DIKULTUR DENGAN PENAMBAHAN GLUTATHIONE SECARA IN VITRO

JURNAL PETERNAKAN ◽

10.24014/jupet.v13i1.2384 ◽

2016 ◽

Vol 13 (1) ◽

pp. 12

Author(s):

Harissatria Harissatria ◽

John Hendri

Keyword(s):

Oxidative Stress ◽

Significant Influence ◽

Embryo Culture ◽

Culture Medium ◽

Culture Media ◽

Lower Percentage ◽

Stage Embryo ◽

Morula Stage ◽

Materials Used

The concentration of high fat content in buffalo embryo culture media is very influential on the increase in oxidative stress that occurs in conditions of in vitro. The occurrence of increased oxidative stress in the process of embryo culture in vitro, would result in lower percentage of embryo culture until the morula stage (32) cells. The purpose of this study was to determine the percentage of fertilized oocytes supplemented with glutathione. To know the development of the embryo until the morula stage (32) cells were cultured in the culture medium supplemented with glutathione (GSH). To determine the viability of morula stage embryo or 32 cells. The materials used in this study is ovarian buffaloes and methods used in this study is an experimental method in the laboratory. Based on these results it can be concluded that the addition of glutathione 1.5 mM in media oocyte maturation buffalo in vitro provide a significant influence on the percentage of maturation ie (P <0:01) or 62.5% and in line with the high percentage of oocytes matured in treatment increase glutathione 1.5 mM, then the percentage of oocytes were successfully fertilized also higher, namely 88.98%. Furthermore, the addition of 3 mM glutathione in embryo culture media in vitro buffalo give a significant influence on the percentage of embryo development to the morula stage or cell 32, namely (P <0.01) in, or 40.73%.

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