Inactivation and oxidative modification of Cu,Zn superoxide dismutase by stimulated neutrophils: The appearance of new catalytically active structures

1992 ◽  
Vol 189 (2) ◽  
pp. 1129-1135 ◽  
Author(s):  
Boris P. Sharonov ◽  
Irina V. Churilova
Keyword(s):  
Superoxide Dismutase ◽  
Oxidative Modification ◽  
Active Structures ◽  
Catalytically Active

Abstract 34: Endogenous Superoxide Dismutase Protects From Impaired Generation of Activated Protein C and Enhanced Susceptibility to Experimental Thrombosis in Mice

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Sanjana Dayal ◽  
Sean X Gu ◽  
Katinan M Wilson ◽  
Ryan Hutchins ◽  
Steven R Lentz
Keyword(s):  
Superoxide Dismutase ◽  
Protein C ◽  
Activated Protein C ◽  
Vena Cava ◽  
Oxidative Modification ◽  
Mrna Levels ◽  
Endothelial Protein C Receptor ◽  
Experimental Thrombosis

In vitro studies have suggested that reactive oxygen species such as superoxide can produce prothrombotic effects, including enhanced platelet activation, increased tissue factor (TF) expression, and an oxidative modification in thrombomodulin impairing its capacity to enhance the generation of activated protein C (APC) by thrombin. It is not known, however, if elevated levels of superoxide accelerate susceptibility to experimental thrombosis in vivo . We used mice genetically deficient in superoxide dismutase-1 (SOD1, an antioxidant enzyme that dismutates superoxide to hydrogen peroxide), to test the hypothesis that lack of SOD1 enhances susceptibility to thrombosis. Susceptibility to carotid artery thrombosis in a photochemical injury model demonstrated that Sod1-/- mice formed stable occlusions significantly faster than Sod1+/+ mice (P<0.05). In an inferior vena cava (IVC) stasis model Sod1- /- mice developed significantly larger thrombi 48 hours after IVC ligation (P<0.05 vs. Sod1+/+ mice). After activation with thrombin (0.5 U/ml) or convulxin (200 ng/ml), no differences in surface expression of P-selectin or binding of fibrinogen were observed between platelets from Sod1-/- and Sod1+/+ mice. The expression of TF mRNA in lung measured by real time qPCR showed similar levels in Sod1-/- and Sod1 +/+ mice. However, the activation of exogenous protein C by thrombin in lung homogenates was decreased in Sod1 -/- mice (P<0.05 vs. Sod1 +/+ mice). Further, in vivo generation of activated protein C in response to thrombin (40 U/Kg) infusion was significantly lower in Sod1-/- mice (P<0.05 vs. Sod1+/+ mice). No differences in mRNA levels for thrombomodulin or endothelial protein C receptor were detected in Sod1 -/- mice vs. Sod1 +/+ mice, suggesting that altered generation of activated protein C in Sod1-/- mice may be related to a direct oxidative effect on thrombomodulin. In accordance, thrombomodulin treated with xanthine/hypoxanthine showed 40% loss of ability to activate protein C that was overcome by addition of SOD and catalase (P<0.05). We conclude that endogenous SOD1 in mice protects from impaired generation of activated protein C and accelerated thrombosis.

scholarly journals Prognostic value of the parameters of free radical oxidation in traumatic brain injury

2016 ◽  
Vol 62 (1) ◽  
pp. 107-111
Author(s):  
E.I. Lvovskaya ◽  
N.V. Derginskyi ◽  
V.A. Sadova ◽  
D.B. Symnaya
Keyword(s):  
Traumatic Brain Injury ◽  
Antioxidant Activity ◽  
Cerebrospinal Fluid ◽  
Superoxide Dismutase ◽  
Brain Injury ◽  
Xanthine Oxidase ◽  
Lipid Peroxides ◽  
Reciprocal Relationship ◽  
Oxidative Modification ◽  
Reference Level

The dynamics of lipoperoxides content and activity of antioxidant (glutathione peroxidase, superoxide dismutase, catalase) and prooxidant (xanthine oxidase) enzymes were investigated in the blood and cerebrospinal fluid of patients with traumatic brain injury of various severity depending on the left- or right-hemisphere localization of injuries. Reciprocal relationship between lipid peroxidation and oxidative modification of proteins from first to 14th day, increase of the level of total antioxidant activity, accompanied with the growth of GP and catalase activity, against the background of decrease in SOD activity from 1 to 7 day have been revealed. Were set lower “average” content of lipid peroxides in the blood and cerebrospinal fluid of patients with the subsequent development of lethal results in compare with cases of favorable outcomes, decrease of geptanofilic lipid peroxides in serum below the reference level, as well as the reduction of antioxidant activity in the blood and cerebrospinal fluid, associated with a sharp falling in superoxide dismutase activity and a significant increase of xanthine oxidase activity, which preceded the lethal results.

Sequential Electrodeposition of Bifunctional Catalytically Active Structures in MoO 3 /Ni–NiO Composite Electrocatalysts for Selective Hydrogen and Oxygen Evolution

2020 ◽  
Vol 32 (39) ◽  
pp. 2003414 ◽  
Author(s):  
Xiaopeng Li ◽  
Yang Wang ◽  
Jiajun Wang ◽  
Yumin Da ◽  
Jinfeng Zhang ◽  
...  
Keyword(s):  
Oxygen Evolution ◽  
Active Structures ◽  
Catalytically Active

INACTIVATION OF MANGANESE SUPEROXIDE DISMUTASE BY IRREVERSIBLE COVALENT OXIDATIVE MODIFICATION IN CARDIOVASCULAR DISEASE

2020 ◽  
Vol 75 (11) ◽  
pp. 1038
Author(s):  
Daniel R. Anderson ◽  
Michael Duryee ◽  
Jake Walker ◽  
Johnathan H. Hall ◽  
Geoffrey M. Thiele ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Oxidative Modification ◽  
Manganese Superoxide

scholarly journals Influence of liposomal drug on the intensity of proteins oxide modification processes in subclinical mastitis of cows

2021 ◽  
Vol 23 (2) ◽  
pp. 3-7
Author(s):  
V. A. Chepurna ◽  
◽  
T. M. Suprovych ◽  
O. I. Vishchur ◽  
V. P. Mizik ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Somatic Cells ◽  
Experimental Studies ◽  
Study Drug ◽  
Subclinical Mastitis ◽  
Oxidative Modification ◽  
Control Group ◽  
Antioxidant Protection ◽  
Liposomal Drug ◽  
Intracisternal Injection

The article contains the experimental studies of the liposomal drug based on plant raw materials ― hypericum (Hypericum perforatum L.) effect on the intensity of oxidative modification of proteins (OMP) in the blood and milk of cows with subclinical mastitis. Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP370 and ketone-derived OMP430, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals. In the milk of sick cows, the content of derivatives OMP370 and OMP430 was1.99 and 2.29 times higher, respectively, than in animals of the control group. At the beginning of the study sick cows’ milk was recorded a significantly low value of the activity of the enzymatic link of antioxidant protection ― superoxide dismutase. At the same time, a 2.6-fold (P<0.001) increase in the number of somatic cells was noted compared to their number in the milk of clinically healthy cows. Intracisternal injection of liposomal drug to cows caused a decrease in the intensity of oxidative processes. In the blood of sick cows the content of aldehyde derivatives OMP370 on the 9th day of the experiment was 23.1% (P<0.05) less than before the drug, and in milk the content of OMP370 decreased by 61.8% (P<0.01). Similar changes were observed with respect to the level of ketone derivatives. In particular, on the 9th day of the experiment, the content of OMP430 decreased by 11.7% (P<0.05) compared with its value in the blood of sick animals before the introduction of the study drug, and in milk it decreased by 64.2% (P<0.01). During the treatment on the 9th day of the experiment, the number of somatic cells in milk decreased by 41.8% (P<0.01). In the course of treatment on the 3rd and 9th day there was a tendency to increase superoxide dismutase activity in the milk of sick cows compared with the beginning of the experiment. Thus, intracisternal injection of liposomal drug to cows with subclinical mastitis leads to a decrease in aldehyde and ketone derivatives of proteins oxidative modification in serum and milk. At the same time, an increase in the activity of the enzymatic link of antioxidant protection and a decrease in the number of somatic cells in the milk of cows were recorded.

scholarly journals Activity of free radical oxidation processes in rats with paracetamol hepatitis and correction with shiitake mushrooms thick extract

2021 ◽  
pp. 81-90
Author(s):  
І. І. Герасимець ◽  
Л. С. Фіра ◽  
І. І. Медвідь
Keyword(s):  
Superoxide Dismutase ◽  
Body Weight ◽  
Free Radical ◽  
Acute Hepatitis ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Modification ◽  
Male Rats ◽  
Defense System ◽  
Shiitake Mushrooms

The antioxidant defense system controls and inhibits all stages of free radical formation reactions, which start from their initiation and end with the formation of hydroperoxides and malonic dialdehyde. Disorders of the body's antioxidant defense system can be caused by both endogenous and exogenous factors and can lead to the oxidative stress development. For the purpose of the negative impact minimizing and correction of the existing disorders, both natural and synthetic origin antioxidants are used. Preference is given to antioxidants of natural origin, due to their mild action and minimal side effects. Shiitake mushrooms are known in China and Japan as a product that has nutritional and medicinal value, for thousands of years. They contain an extremely rich complex of various biologically active substances that can have antioxidant, hepatoprotective effect and influence on the liver diseases pathogenesis. The aim of the work was to investigate the effect of shiitake mushrooms thick extract on the activity of free radical processes and indicators of the antioxidant system under the conditions of paracetamol hepatitis in rats. The experimental work was being conducted on the white male rats, which were divided into 10 groups of 6 animals each. Acute hepatitis was simulated by intragastric administration of paracetamol in a dose of 1 250 mg/kg 1 time per day (for 2 days) as a suspension in 2% starch gel solution. We investigated the effect of shiitake mushrooms thick extract on the induced pathology, which was administered intragastrically 2 hours before the paracetamol introduction and daily after the lesion in a dose of 150 mg/kg of the rat’s body weight. Silibor was selected as the comparison drug, which was administered according to the same scheme like the investigated extract in a dose of 20 mg/kg of the animal’s body weight. Euthanasia was conducted on the 3rd, 7th and 10th day after the onset of the lesion. Liver homogenate and animal serum were examined. The effectiveness of shiitake mushrooms thick extract was evaluated under the conditions of the caused pathology by superoxide dismutase and catalase activity, as well as by the content of TBA-active and proteins oxidative modification products. An increasing of the TBA-active products content and the products of proteins oxidative modification in the serum and liver of animals after the toxic paracetamol exposure testifies to the liver damage and the development of acute hepatitis in rats. Decreased activity of antioxidant enzymes such as catalase and superoxide dismutase was also noted. An investigation, which was conducted by us, allowed us to reveal a positive effect of shiitake mushrooms thick extract on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of white rats paracetamol lesion.

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