Influence of the immunoregulatory serum lipoprotein LDL-In on the in vivo proliferation and differentiation of antigen-binding and antibody-secreting lymphocytes during a primary immune response

1980 ◽  
Vol 49 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Linda K. Curtiss ◽  
David H. DeHeer ◽  
Thomas S. Edgington
Keyword(s):  
Immune Response ◽  
Serum Lipoprotein ◽  
Primary Immune Response ◽  
Antigen Binding ◽  
Proliferation And Differentiation

Effects of a primary immune response to T-cell dependent antigen on serotonin metabolism in frontal cortex: in vivo microdialysis study in freely moving Fischer 344 rat

1994 ◽  
Vol 645 (1-2) ◽  
pp. 150-156 ◽  
Author(s):  
Alain M. Gardier ◽  
Sébastien Kachaner ◽  
Elisabeth Khan Shaghaghi ◽  
Christian Blot ◽  
Claude Bohuon ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
In Vivo Microdialysis ◽  
Primary Immune Response ◽  
Serotonin Metabolism ◽  
Fischer 344 ◽  
Fischer 344 Rat ◽  
Freely Moving ◽  
Microdialysis Study

scholarly journals P044 Milk derived exosomes has a therapeutic effect on experimental colitis

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S155-S155
Author(s):  
R Golan-Gerstl ◽  
N Koroukhov ◽  
Y Elbaum Shiff ◽  
I Shilo ◽  
S Reif
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Therapeutic Effect ◽  
Experimental Colitis ◽  
Intestinal Epithelial ◽  
Mice Model ◽  
High Concentration ◽  
Fluorescent Signal ◽  
Proliferation And Differentiation

Abstract Background Inflammatory bowel disease (IBD) is a complex disorder that results from a dysregulated immune response in the gut. Emerging therapy for IBD treatment is mainly focused on regulation of the immune response. Exosomes are nanovesicle packing different molecules such as miRNAs that transfer their cargo to recipient cells. We and others found that mammalian milk contain high concentration of exosomes (milk-derived exosomes, MDE) carrying beneficial miRNAs such as miRNA-148. Furthermore, MDE are taken up by different cell type such as intestinal epithelial cells, modify target gene expression, promote proliferation and differentiation of colon epithelial cells. The aim of this study is to explore the therapeutic effect of MDE on colitis. Methods We used gavage administration of MDE labelled with DiR dye to track their localisation patterns in vivo. The therapeutic effect of MDE on colitis was study in mice model of SDS induced colitis, colon length, histopathological scoring grade, cytokine expression, stool consistency and miRNA expression were analysed. Results Imaging of mouse that have receive labelled MDE revealed an accumulation of fluorescent signal in the intestine. Moreover, fluorescent signal in the intestine and liver is time dependent. MDE reduced the histopathological scoring grade from 5.83 ± 1.47 to 0.6 ± 0.6, p < 0.05. The length of the colon of MDE-treated animals was 7.9 ± 0.19 in comparison to 6.92 ± 0.3 p < 0.05 of the untreated. The weight loss as a result of the colitis was reverted in MDE-treated mice. Likewise, MDE treatment reduced IL-6, TNF-α and caveolin expression from 3.83 to 0.78, 1.59 to 0.86 and 3.52 to 1.1, respectively. Highly expressed miRNAs (miRNA-320, 375, Let-7a and 6073) were found to be more abundant in colon of MDE treatment mice compared with the untreated. Conclusion This study demonstrated that MDE have a therapeutic effect on colitis in vivo. Proving the effect of MDE on colitis will have implications for the potential of adding MDE as a therapeutic nutrient to be included in the formulas for IBD patients.

scholarly journals Antigen binding and idiotype analysis of antibodies obtained after electroporation of heavy and light chain genes encoding phosphocholine-specific antibodies: a model for T15-idiotype dominance.

1992 ◽  
Vol 176 (6) ◽  
pp. 1637-1643 ◽  
Author(s):  
J J Kenny ◽  
C M Moratz ◽  
G Guelde ◽  
C D O'Connell ◽  
J George ◽  
...  
Keyword(s):  
Immune Response ◽  
Amino Acid ◽  
B Cell ◽  
Molecular Model ◽  
Immunoglobulin M ◽  
Single Amino Acid ◽  
Primary Immune Response ◽  
Antigen Binding ◽  
Large Numbers ◽  
Genes Encoding

Antibodies bearing the T15 idiotype dominate the murine primary immune response to phosphocholine (PC). Analysis of antigen binding of antibodies derived from V1:DFL16.1:JH1 (VH1) germline and N region-derived variant heavy (H) chains and kappa 22, kappa 24, and kappa 8 light (L) chains demonstrates that the T15H:kappa 22L (T15) antibody binds PC at least 20-40 times better than other antibodies derived from alternate germline forms of the VH1 H chain and kappa 22, kappa 24, or kappa 8 L chains. To achieve affinities in the same range as the T15 antibody, kappa 24 and kappa 8 L chain-containing antibodies must have H chains derived from variant N region or somatically mutated VH1 genes. Single amino acid differences at the VD junction of the various germline and N region variant VH1 H chains dictate the L chain that can associate with the H chain to produce a PC-specific antibody. Several H:L combinations give rise to T15 or M167 idiotype-positive antibodies that lack specificity for PC, and single amino acid substitutions or insertions at the VH1:D junction result in the loss of T15 or M167 idiotopes. Based on these observations, our data support a molecular model involving both preferential gene rearrangement and antigen-driven B cell selection to explain T15 idiotype dominance in the immune response to PC. In the absence of N region diversification, large numbers of neonatal B cells bearing the T15H:kappa 22L surface immunoglobulin M (sIgM) receptors would be selected and expanded by autologous or environmental PC antigen into the long-lived peripheral B cell pool.

scholarly journals L1 Antibodies Block Lymph Node Fibroblastic Reticular Matrix Remodeling In Vivo

1998 ◽  
Vol 187 (12) ◽  
pp. 1953-1963 ◽  
Author(s):  
Gino Di Sciullo ◽  
Tim Donahue ◽  
Melitta Schachner ◽  
Steven A. Bogen
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Primary Immune Response ◽  
Immunoglobulin Superfamily ◽  
Matrix Remodeling ◽  
Protein Antigens ◽  
Cellular Processes ◽  
Reticular System ◽  
Macrophage Accumulation

L1 is an immunoglobulin superfamily adhesion molecule highly expressed on neurons and involved in cell motility, neurite outgrowth, axon fasciculation, myelination, and synaptic plasticity. L1 is also expressed by nonneural cells, but its function outside of the nervous system has not been studied extensively. We find that administration of an L1 monoclonal antibody in vivo disrupts the normal remodeling of lymph node reticular matrix during an immune response. Ultrastructural examination reveals that reticular fibroblasts in mice treated with L1 monoclonal antibodies fail to spread and envelop collagen fibers with their cellular processes. The induced defect in the remodeling of the fibroblastic reticular system results in the loss of normal nodal architecture, collapsed cortical sinusoids, and macrophage accumulation in malformed sinuses. Surprisingly, such profound architectural abnormalities have no detectable effects on the primary immune response to protein antigens.

scholarly journals CD8+ T-cell selection, function, and death in the primary immune response in vivo

2000 ◽  
Vol 106 (10) ◽  
pp. 1251-1261 ◽  
Author(s):  
Margaret F.C. Callan ◽  
Chrysoula Fazou ◽  
Hongbing Yang ◽  
Tim Rostron ◽  
Kathryn Poon ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Cd8 T Cell ◽  
Primary Immune Response ◽  
Selection Function ◽  
Cell Selection ◽  
T Cell Selection

scholarly journals Oligoclonal expansion of major histocompatibility complex class I-restricted cytolytic T lymphocytes during a primary immune response in vivo: direct monitoring by flow cytometry and polymerase chain reaction.

1993 ◽  
Vol 177 (5) ◽  
pp. 1487-1492 ◽  
Author(s):  
H R MacDonald ◽  
J L Casanova ◽  
J L Maryanski ◽  
J C Cerottini
Keyword(s):  
Flow Cytometry ◽  
Immune Response ◽  
Polymerase Chain Reaction ◽  
Cytotoxic T Lymphocyte ◽  
Cell Receptor ◽  
Primary Response ◽  
Primary Immune Response ◽  
Chain Reaction ◽  
Polymerase Chain

Previous T cell receptor (TCR) sequence analysis of a panel of 23 H-2Kd restricted cytotoxic T lymphocyte (CTL) clones recognizing the decapeptide HLA-CW3 170-179 revealed a striking conservation of TCR structure, in that all clones examined used V beta 10 and J alpha pHDS58 segments. We show here that the primary response in vivo after intraperitoneal injection of DBA/2 mice with HLA-CW3 expressing transfectants of syngeneic P815 (H-2d) tumor cells is characterized by a dramatic expansion of CD8+ V beta 10+ CTL in the peritoneal cavity and draining (mesenteric) lymph node, as well as in peripheral blood. Additional analysis of TCR on HLA-CW3 immune populations by flow cytometry and polymerase chain reaction further indicates that the vast majority of responding CD8+ cells express restricted V alpha domains, a dominant J alpha segment (pHDS58), and a conserved CDR3 length for both alpha and beta chains. This novel system provides a unique opportunity to directly monitor an oligoclonal primary antigen specific immune response in vivo at the single cell level independently of functional assays.

scholarly journals Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

2020 ◽  
Vol 21 (3) ◽  
pp. 890 ◽  
Author(s):  
Piotr Kaczynski ◽  
Stefan Bauersachs ◽  
Monika Baryla ◽  
Ewelina Goryszewska ◽  
Jolanta Muszak ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Adhesion ◽  
Lipid Metabolism ◽  
Early Pregnancy ◽  
Valuable Resource ◽  
Proliferation And Differentiation ◽  
Induced Changes ◽  
Estradiol 17Β ◽  
In Pregnancy

Estradiol-17β (E2) is a key hormone regulating reproductive functions in females. In pigs, E2, as the main conceptus signal, initiates processes resulting in prolonged corpus luteum function, embryo development, and implantation. During early pregnancy the endometrium undergoes morphological and physiological transitions that are tightly related to transcriptome changes. Recently, however, the importance of E2 as a primary conceptus signal in the pig has been questionable. Thus, the aim of the present study was to determine the effects of E2 on the porcine endometrial transcriptome in vivo and to compare these effects with transcriptome profiles on day 12 of pregnancy. Microarray analysis revealed differentially expressed genes (DEGs) in response to E2 with overrepresented functional terms related to secretive functions, extracellular vesicles, cell adhesion, proliferation and differentiation, tissue rearrangements, immune response, lipid metabolism, and many others. Numerous common DEGs and processes for the endometrium on day 12 of pregnancy and E2-treated endometrium were identified. In summary, the present study is the first evidence for the effect of E2 on transcriptome profiles in porcine endometrium in vivo in the period corresponding to the maternal recognition of pregnancy. The presented results provide a valuable resource for further targeted studies considering genes and pathways regulated by conceptus-derived estrogens and their role in pregnancy establishment.

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