Atomic Force Microscopy (AFM) and X-Ray Micro-Computed Tomography (Micro-CT): Applications in Cell Wall Imaging of Softening Fruit

2019 ◽  
pp. 8-14
Author(s):  
Jovyn K.T. Frost ◽  
Roswitha Schröder
Keyword(s):  
Computed Tomography ◽  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Wall Imaging

scholarly journals Antimicrobial Silver-Polyethyleneimine-Polylactic Acid Polymer Composite Film for Coating Methacrylate-Based Denture Surfaces

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Zoltán Géczi ◽  
Péter Hermann ◽  
László Kőhidai ◽  
Orsolya Láng ◽  
Zsófia Kőhidai ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Atomic Force Microscopy ◽  
Composite Film ◽  
Polylactic Acid ◽  
Polymer Composite ◽  
Water Soluble ◽  
Micro Computed Tomography ◽  
Denture Stomatitis ◽  
Force Microscopy ◽  
Atomic Force

To prepare an antimicrobial polymer composite composed of silver- (Ag-) polyethyleneimine- (PEI-) polylactic acid (PLA) in chloroform, for coating the mucosal surfaces of methacrylate-based dentures as a prospective therapy for denture stomatitis.The water-insoluble, tightly bound, hard, micrometre-thin, and colourless film exerts its effects by direct contact with the pathogens and via the active constituents (Ag, PEI, and Ag-PEI) released slowly into the mucosa’s salivary layer. Silver and PEI were blended at 140°C, then bound to PLA. The Ag-PEI complex was characterised by dynamic light scattering and transmission electron microscopy, and the Ag-PEI-PLA composite was examined by atomic force microscopy and micro-computed tomography. The characteristic was measured by atomic force microscopy (AFM) and micro-computed tomography (micro-CT). The quantity of water-soluble Ag-PEI complex released from the composite film was measured with gravimetry. The cellular physiological effects were analysed by impedimetry and computer-based morphometry using human gingival epithelial cells. A real-time cell proliferation assay revealed moderate toxic effects of Ag-PEI on the epithelium. The viscous Ag-PEI-PLA solution produced could be applied as a thin film on methacrylate surfaces. Active antimicrobial components (Ag, PEI, and Ag-PEI) were released from the hard, tightly bound Ag-PEI-PLA coating. This study’s findings verified the applicability of the antimicrobial Ag-PEI-PLA composite for coating the inner surfaces of acrylate dentures. Owing to the well-known antimicrobial effects of silver and PEI and the supplementary effects of chloroform, this composite provides a new therapeutic method for denture stomatitis that can be easily performed by dentists.

Image of the Micro-Computed Tomography and Atomic-Force Microscopy of Bone in Osteoporosis Animal Model

2018 ◽  
Vol 18 (10) ◽  
pp. 6726-6731 ◽  
Author(s):  
Gyeong-Ji Kim ◽  
Han Seok Yoo ◽  
Kwon Jai Lee ◽  
Jeong Woo Choi ◽  
Jeung Hee An
Keyword(s):  
Computed Tomography ◽  
Atomic Force Microscopy ◽  
Animal Model ◽  
Micro Computed Tomography ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Biomimetic Surfaces Coated with Covalently Immobilized Collagen Type I: An X-Ray Photoelectron Spectroscopy, Atomic Force Microscopy, Micro-CT and Histomorphometrical Study in Rabbits

2019 ◽  
Vol 20 (3) ◽  
pp. 724 ◽  
Author(s):  
Antonio Scarano ◽  
Felice Lorusso ◽  
Tiziana Orsini ◽  
Marco Morra ◽  
Giorgio Iviglia ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Photoelectron Spectroscopy ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Micro Ct ◽  
Implant Surface ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force

Background: The process of osseointegration of dental implants is characterized by healing phenomena at the level of the interface between the surface and the bone. Implant surface modification has been introduced in order to increase the level of osseointegration. The purpose of this study is to evaluate the influence of biofunctional coatings for dental implants and the bone healing response in a rabbit model. The implant surface coated with collagen type I was analyzed through X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), micro-CT and histologically. Methods: The sandblasted and double acid etched surface coated with collagen type I, and uncoated sandblasted and double acid etched surface were evaluated by X-ray Photoelectron Spectroscopy (XPS) and Atomic Force Microscopy (AFM) analysis in order evaluate the different morphology. In vivo, a total of 36 implants were positioned in rabbit articular femoral knee-joint, 18 fixtures for each surface. Micro-CT scans, histological and histomorphometrical analysis were conducted at 15, 30 and 60 days. Results: A histological statistical differences were evident at 15, 30 and 60 days (p < 0.001). Both implant surfaces showed a close interaction with newly formed bone. Mature bone appeared in close contact with the surface of the fixture. The AFM outcome showed a similar roughness for both surfaces. Conclusion: However, the final results showed that a coating of collagen type I on the implant surface represents a promising procedure able to improve osseointegration, especially in regions with a low bone quality.

Nanocrystalline Solutions as Precursors to The Spray Deposition of Cdte Thin Films

1995 ◽  
Vol 382 ◽  
Author(s):  
Martin Pehnt ◽  
Douglas L. Schulz ◽  
Calvin J. Curtis ◽  
Helio R. Moutinho ◽  
Amy Swartzlander ◽  
...  
Keyword(s):  
Thin Films ◽  
Atomic Force Microscopy ◽  
Grain Size ◽  
X Ray Diffraction ◽  
X Ray ◽  
Force Microscopy ◽  
Cdte Nanoparticles ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Cdte Thin Films

ABSTRACTIn this article we report the first nanoparticle-derived route to smooth, dense, phase-pure CdTe thin films. Capped CdTe nanoparticles were prepared by injection of a mixture of Cd(CH3)2, (n-C8H17)3 PTe and (n-C8H17)3P into (n-C8H17)3PO at elevated temperatures. The resultant nanoparticles 32-45 Å in diameter were characterized by x-ray diffraction, UV-Vis spectroscopy, transmission electron microscopy, thermogravimetric analysis and energy dispersive x-ray spectroscopy. CdTe thin film deposition was accomplished by dissolving CdTe nanoparticles in butanol and then spraying the solution onto SnO2-coated glass substrates at variable susceptor temperatures. Smooth and dense CdTe thin films were obtained using growth temperatures approximately 200 °C less than conventional spray pyrolysis approaches. CdTe films were characterized by x-ray diffraction, UV-Vis spectroscopy, atomic force microscopy, and Auger electron spectroscopy. An increase in crystallinity and average grain size as determined by x-ray diffraction was noted as growth temperature was increased from 240 to 300 °C. This temperature dependence of film grain size was further confirmed by atomic force microscopy with no remnant nanocrystalline morphological features detected. UV-Vis characterization of the CdTe thin films revealed a gradual decrease of the band gap (i.e., elimination of nanocrystalline CdTe phase) as the growth temperature was increased with bulk CdTe optical properties observed for films grown at 300 °C.

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