Transgenic chicken/poultry birds: serving us for survival

2020 ◽  
pp. 211-221
Author(s):  
Afsaneh Golkar-Narenji ◽  
James N. Petitte ◽  
Paul E. Mozdziak
Keyword(s):  
Transgenic Chicken ◽  
Poultry Birds

A Cross Sectional Survey of Chicken Astroviruses Antibody in Broiler and Sonali (Cross-Bred) Chickens in Selected Areas in Bangladesh

2020 ◽  
Vol 43 (1) ◽  
pp. 75-80
Author(s):  
Md Zulfekar Ali ◽  
Mohammad Moktader Moula ◽  
Zafar Ahmed Bhuiyan ◽  
Muhammad Tariq Javed
Keyword(s):  
Age Groups ◽  
Winter Season ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Blood Samples ◽  
Elisa Kit ◽  
Published Report ◽  
Poultry Birds ◽  
Wide Range ◽  
Group B

AbstractChicken astroviruses (CAstV) are enteric viruses of poultry causing gastroenteritis, malabsorption, gout and white chick disease commonly known as runting-stunting syndrome (RSS). It can affect the wide range of poultry birds, especially chicken, turkey and duck worldwide. To our best knowledge there is no published report on presence of antibodies against CAstV in Bangladesh. Therefore, the study aimed to detect the presence of CAstV antibodies in broilers and sonali chickens (a cross-bread) in Bangladesh through a cross-sectional survey. A total of 454 blood samples from 66 flocks of broiler (n=343) and sonali chickens (n=111) of different ages were obtained during 2017 from four districts. The birds were healthy but were not vaccinated against CAstV. The samples were tested for specific antibodies against CAstV Group B by using commercially available ELISA kit. Overall, 16.74% (76/454) samples and 34.84% (23/66) flocks were positive for CAstV antibodies. The seroprevalence of CAstV was significantly (p=0.001) higher in sonali chickens (36.96%) than broiler (10.20%), while it was significantly higher (p=0.001) in birds of Bogura district (36.94%) than the other three districts. Regarding the age groups, seroprevalence was insignificantly (p=0.192) higher in sonali chicken before laying age (45%) than during laying age (27.45%). Regarding the seasons, CAstV infection was prevalent significantly (p=0.001) higher in winter season. Thus, the present study indicated the presence of CAstV in poultry in Bangladesh, so further studies are required to find out the magnitude of the problem in the country.

scholarly journals Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 38
Author(s):  
Takehiro Mukae ◽  
Sho Okumura ◽  
Takuma Watanobe ◽  
Kyoko Yoshii ◽  
Takahiro Tagami ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Binding Capacity ◽  
Specific Binding ◽  
Egg White ◽  
Peptide Sequence ◽  
Efficient Production ◽  
Antigen Binding ◽  
Transgenic Chicken ◽  
Bioreactor System ◽  
Transgenic Chickens

Increased commercial demand for monoclonal antibodies (mAbs) has resulted in the urgent need to establish efficient production systems. We previously developed a transgenic chicken bioreactor system that effectively produced human cytokines in egg whites using genome-edited transgenic chickens. Here, we describe the application of this system to mAb production. The genes encoding the heavy and light chains of humanized anti-HER2 mAb, linked by a 2A peptide sequence, were integrated into the chicken ovalbumin gene locus using a CRISPR/Cas9 protocol. The knock-in hens produced a fully assembled humanized mAb in their eggs. The mAb expression level in the egg white was 1.4–1.9 mg/mL, as determined by ELISA. Furthermore, the antigen binding affinity of the anti-HER2 mAb obtained was estimated to be equal to that of the therapeutic anti-HER2 mAb (trastuzumab). In addition, antigen-specific binding by the egg white mAb was demonstrated by immunofluorescence against HER2-positive and -negative cells. These results indicate that the chicken bioreactor system can efficiently produce mAbs with antigen binding capacity and can serve as an alternative production system for commercial mAbs.

Molecular Detection and Phylogenetic Analysis of Chicken Astrovirus Associated with Poultry Enteritis

2021 ◽  
Author(s):  
C. Patidar ◽  
D.K. Sharma ◽  
R. Singathia ◽  
P. Suthar ◽  
A. Saraswat ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Histopathological Examination ◽  
Villous Atrophy ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Amino Acid Sequences ◽  
Poultry Birds ◽  
Commercial Poultry ◽  
Liver Specimen

Background: Poultry enteritis is an important multifactorial disease. Chicken Astrovirus (CAstV) usually associated with enteritis. The aim of this study was to investigate the occurrence of CAstV in poultry enteritis cases, its molecular characterization, phylogenetic analysis and gross and microscopic examination of intestine and liver specimen affected with CAstV. Methods: Total 604 dead poultry birds from commercial poultry farms affected with enteritis were examined for presence of CAstV. Intestinal samples of four birds were pooled to make one biological sample. CAstV was detected by reverse transcriptase PCR (RT-PCR) using ORF-1b gene specific primers. Molecular characterization was carried out by partial gene sequencing. Result: CAstV was detected in 20.52% (31/151) of samples. Highest prevalence (49.29%) was observed in 0-1 week old chicks. The partial molecular characterization revealed high similarity of the nucleotide sequence from India (97% to 93%) and from USA, Brazil, Poland and Korea (94 to 92%). Further similarity of amino acid sequences of CAstV from India (100% to 98%) and from USA, Brazil, Poland and Korea (98 to 97%) was observed. Histopathological examination revealed villous atrophy, congestion and atrophic cystic glands in sub-mucosa of intestine. Further severe congestion and hemorrhages along with infiltration of inflammatory cells in liver parenchyma was observed.

scholarly journals Comparative behaviour of local and exotic chickens in tests of fearfulness, anxiety and cognition

2021 ◽  
Vol 41 (1) ◽  
pp. 4-15
Author(s):  
T. B. Raji ◽  
A. A. Toye
Keyword(s):  
Production System ◽  
Genetic Selection ◽  
Test Results ◽  
Extensive Management ◽  
Quantitative Genetic ◽  
Voluntary Approach ◽  
Ongoing Effort ◽  
Poultry Birds ◽  
Distress Calls ◽  
Two Phases

Behaviour affects performance and productivity of poultry birds especially chickens, some behavioural traits are advantageous in a particular production system and may be of disadvantage in another production system. The present study compared behavioural of Nigeria Local Chicken, NLC (two separate samples of 11 Yoruba Ecotype) and its Exotic counterparts (11 Broilers and 11 Pullets) by use of the Open Field (OFT), T-Maze, Forced Approach, and Voluntary Approach Tests (FAT and VAT respectively) during two phases of Growth (0-4 Weeks, and 4-8 weeks age respectively). The former group (NLC) is better adapted to extensive management in the Nigerian Guinea savannah than the latter Results showed that Yoruba NLC issued a significantly (p<0.05) higher number of distress calls than the Exotic genotypes in the OFT at age 7 and 48 days, and the NLC issued significantly more calls at 7 days age. Broilers exhibited significantly lower OFT Latency at 7 and 48 days, and Broilers traversed fewer squares and spent less time ambulating than other genotypes at 48 days age. Ina T-maze, Broilers showed significantly (p<0.05) lower exploratory behaviour than other groups (higher latency to leave the start box). In the FAT, NLC showed lower Latency to flight (p<0.05) than the Exotic genotypes. Ethological test results indicate differences in the behavioural characters exhibited by Yoruba NLC and Exotic Chickens and such differences could embody the basis of anecdotal differences in the rates of survival under extensive management conditions, and may be subjected to quantitative genetic selection in the ongoing effort to produce improved chickens that incorporate a combination of desirable traits from both Local and Exotic chickens.

scholarly journals Sero-prevalence of Toxoplasma gondii infection in backyard poultry birds of Tarai Zone of West Bengal, India

2017 ◽  
Vol 7 (9) ◽  
pp. 544-547 ◽  
Author(s):  
Dhananjay Kumar ◽  
◽  
Surajit Baidya ◽  
Ruma Jas ◽  
Soumitra Pandit ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
West Bengal ◽  
Backyard Poultry ◽  
Poultry Birds ◽  
Toxoplasma Gondii Infection

scholarly journals Prevalence of Menopon gallinae Linn. (Insecta, Phthiraptera, Menoponidae, Amblycera) upon poultry birds (Gallus gallus domesticus) of selected locality of district Chamoli Garhwal (Uttarakhand), India

2013 ◽  
Vol 5 (2) ◽  
pp. 400-405 ◽  
Author(s):  
Surman Arya ◽  
Sandeep Negi ◽  
Suneel Kumar Singh
Keyword(s):  
Prevalence Rate ◽  
Good Health ◽  
Gallus Domesticus ◽  
Strong Positive Correlation ◽  
Gallus Gallus Domesticus ◽  
Coding System ◽  
Hygienic Condition ◽  
Infestation Intensity ◽  
Black Feather ◽  
Poultry Birds

Prevalence and intensity of infestation of Menopon gallinae (Linn.) was recorded from 688 poultry birds belonging to 30 selected locality of district Chamoli (Uttarakhand) during March, 2012 to February, 2013, out of which 310 were found infested (45.06%). Prevalence rate were higher in female (46.8%) than male (42%) birds in present investigation. Higher infestation were noted in certain parameters like captivity (larger group), poor health, poor hygienic condition, poor feather condition, black feather colour, desi breed and older birds. Singly reared, good health, good hygienic condition, good feather condition, mix colour, broiler breed and younger poultry birds were found to have least infestation. Intensity of infestation was noted by coding system. Maximum poultry bird bore very light (VL) infestation (29.68%) while very few were found with very heavy (VH) infestation (4.52%). Relative monthly intensity of infestation remained more or less similar in all the categories. Lice were peaked during summer in comparison to the winter. Strong positive correlation existed between monthly lice index and temperature (+0.79); lice index and photoperiod (+0.84), while the same were found insignificant with humidity (-0.23) and rainfall (-0.17).

scholarly journals Performance of Vanaraja and Kaveri poultry birds in tribal villages of Jharkhand

2020 ◽  
Vol 8 (5) ◽  
pp. 1960-1966
Author(s):  
Asit Chakrabarti ◽  
BK Jha ◽  
PK Sarkar ◽  
VK Yadav
Keyword(s):  
Poultry Birds

scholarly journals Backyard Chicken Farming Role in Supplementing Household Economy of District Quetta, Pakistan

2020 ◽  
Vol 8 (3) ◽  
pp. 568
Author(s):  
Kamran Baseer Achakzai ◽  
Muhammad Abbas Shah ◽  
Ramla Achakzai ◽  
Ghulam Hussain Kakar
Keyword(s):  
Rural Areas ◽  
Capital Investment ◽  
Poultry Meat ◽  
Cash Income ◽  
Household Economy ◽  
Backyard Poultry ◽  
Poultry Birds ◽  
Backyard Chicken ◽  
Meat And Eggs ◽  
Poultry Farming

Backyard chicken farming is as an effective tool for poverty reduction in rural areas, by providing living to resource poor communities; it is an important source of extra cash income other than eggs and meat for family consumption. The present study was carried out to assess the role of backyard poultry farming in supplementing household economy of rural farmers dwelling in district Quetta, Pakistan. Information from ninety-nine randomly selected female backyard poultry farmers were gathered during November 2016 to March 2017. Owing to previous government and non-government interventions backyard poultry keeping was found as an established practice. Women were found the main custodian of backyard poultry birds and consequently were the main beneficiary of the activity. Poultry birds of Fayoumi and Golden / Rhode Island Red (RIR) breeds were the main types. Majority of the farmers reared these birds under scavenging conditions in combination with feeding on household left over dry bread (52%); most of the respondents (62%) were taking care of the birds by themselves which resulted in overall low production cost. Income included the earning received from sale of birds, eggs and the home consumption of poultry meat and eggs. Net income per annum per household was estimated as Pakistani Rupee (PKR) 79290/- (US$ 566). The estimated Rank Based Quotients (R.B.Q) based on farmers perception disclosed backyard poultry keeping as an important domestic source of meat and eggs with almost no or very little capital investment, ranked (I, 80%) followed by a substantial source of supplementary income and was ranked (II, 79%). Amongst the problems, disease outbreaks were the most prevailing problem (I, 90%) followed by unavailability of vaccines (II, 85%). The backyard poultry farming generated subsistence income and provided high quality valuable food that improved household nutrition.

Sign in / Sign up

Export Citation Format

Share Document