Fresh capillary blood analysis using darkfield microscopy as a tool for screening nutritional deficiencies of iron and cobalamin (vitamin B12): A validity study

2016 ◽  
Vol 3 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Sheriden Keegan ◽  
Jacinta Arellano ◽  
Tini Gruner
Keyword(s):  
Vitamin B12 ◽  
Capillary Blood ◽  
Blood Analysis ◽  
Nutritional Deficiencies ◽  
Validity Study ◽  
Darkfield Microscopy

scholarly journals Recurrent Aphthous Stomatitis and Nutritional Deficiencies: Report of Three Cases with Literature Review

2019 ◽  
pp. 93-103
Author(s):  
Yadira V. Boza Oreamuno DDS, MSc ◽  
María F. Rugama Flores BSND
Keyword(s):  
Folic Acid ◽  
Iron Deficiency ◽  
Vitamin B12 ◽  
Systemic Disease ◽  
Recurrent Aphthous Stomatitis ◽  
Blood Analysis ◽  
Aphthous Stomatitis ◽  
Vegan Diet ◽  
Nutritional Deficiencies ◽  
First Case

Introduction: Recurrent aphthous stomatitis (RAS) is the most common ulcerative disease that affects the non-keratinized oral mucosa. The etiology is still unknown, but several local and systemic factors have been proposed as causal agents. Case description: three patients are reported with RAS associated with nutritional deficiencies, due to dietary restriction diets. The first case, a 19-year-old woman with iron deficiency, reported taking green tea daily and consuming a small amount of carbohydrates, fats and meat. The second case, a 32-year-old man with iron deficiency and vitamin B12 associated with vegan diet, although sometimes if he consumed meat. The third case, man of 50 years, vegan and ex-smoker for a year, presented deficiency of iron, vitamin B12 and folic acid. In all three cases a detailed questioning, clinical examination and complete blood analysis allowed to establish an adequate diagnosis, dental management and referral with a nutritionist, in order to treat the underlying pathology and not only provide a symptomatic treatment of the injuries. Discussion: In the setting of RAS, it is advisable to perform an analysis that includes a blood count, folic acid, iron and vitamin B12, to rule out possible systemic causes and eventually treat them. Clinical management aims to improve patient function and quality of life through topical and systemic therapies; however, it is essential to identify and control the causal factors that contribute; as well as, the exclusion or treatment of the underlying systemic disease.

scholarly journals Long-Term Iron and Vitamin B12 Deficiency Are Present after Bariatric Surgery, Despite the Widespread Use of Supplements

2021 ◽  
Vol 18 (9) ◽  
pp. 4541
Author(s):  
Mauro Lombardo ◽  
Arianna Franchi ◽  
Roberto Biolcati Rinaldi ◽  
Gianluca Rizzo ◽  
Monica D’Adamo ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Weight Loss ◽  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
Ideal Body Weight ◽  
Excess Weight ◽  
Nutritional Deficiencies ◽  
Average Percentage ◽  
Nutritional Studies

There are few long-term nutritional studies in subjects undergoing bariatric surgery that have assessed weight regain and nutritional deficiencies. In this study, we report data 8 years after surgery on weight loss, use of dietary supplements and deficit of micronutrients in a cohort of patients from five centres in central and northern Italy. The study group consisted of 52 subjects (age: 38.1 ± 10.6 y, 42 females): 16 patients had Roux-en-Y gastric bypass (RYGB), 25 patients had sleeve gastrectomy (SG) and 11 subjects had adjustable gastric banding (AGB). All three bariatric procedures led to sustained weight loss: the average percentage excess weight loss, defined as weight loss divided by excess weight based on ideal body weight, was 60.6% ± 32.3. Despite good adherence to prescribed supplements, 80.7% of subjects (72.7%, AGB; 76.7%, SG; 93.8 %, RYGB) reported at least one nutritional deficiency: iron (F 64.3% vs. M 30%), vitamin B12 (F 16.6% vs. M 10%), calcium (F 33.3% vs. M 0%) and vitamin D (F 38.1% vs. M 60%). Long-term nutritional deficiencies were greater than the general population among men for iron and among women for vitamin B12.

scholarly journals Vitamin B12 Deficiency Is Prevalent Among Czech Vegans Who Do Not Use Vitamin B12 Supplements

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 3019 ◽  
Author(s):  
Eliška Selinger ◽  
Tilman Kühn ◽  
Magdalena Procházková ◽  
Michal Anděl ◽  
Jan Gojda
Keyword(s):  
Czech Republic ◽  
Vitamin B12 ◽  
Binding Capacity ◽  
Iron Status ◽  
Clinical Care ◽  
Vegan Diet ◽  
Cobalamin Deficiency ◽  
Nutritional Deficiencies ◽  
The Czech Republic ◽  
Supplement Use

As not much is known about the prevalence and predictors of nutritional deficiencies among vegans in the Czech Republic, we evaluated whether supplement use and duration of adherence to the vegan diet are associated with the risk of cobalamin and iron deficiencies. Associations between self-reported supplementation and duration of vegan diet with biomarkers of cobalamin (serum cobalamin, holotranscobalamin, homocysteine, folate) and iron status (serum ferritin, iron binding capacity, transferrin and saturation of transferrin) were assessed by cross-sectional analyses of medical data from a clinical nutrition center. Data from 151 (72 females) adult vegans (age 18–67 years), who were free of major chronic diseases and 85 (40 females) healthy non-vegans (age 21–47 years) were analyzed. Overall, vegans had significantly lower cobalamin, hemoglobin and ferritin levels, but higher folate and MCV values compared to non-vegans. Vegans not using cobalamin supplements were at higher risk of low plasma cobalamin than regularly supplementing vegans (OR: 4.41, 95% CI 1.2–16.16 for cobalamin, OR: 19.18, 95% CI 1.02–359.42 for holotranscobalamin), whereas no significant differences in cobalamin status related to duration of the vegan diet were observed. Regularly supplementing vegans had similar levels of cobalamin/holotranscobalamin as non-vegans. Despite lower ferritin and hemoglobin levels, there was no indication of a higher risk of iron-deficiency among vegans. To conclude cobalamin deficiency risk depends on supplementation status and not on the duration of an exclusive vegan diet, which underlines the need to integrate cobalamin status monitoring and counselling on supplement use in routine clinical care in the Czech Republic.

scholarly journals Biomarkers of Micronutrients in Regular Follow-Up for Tyrosinemia Type 1 and Phenylketonuria Patients

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2011 ◽  
Author(s):  
Kimber van Vliet ◽  
Iris L. Rodenburg ◽  
Willem G. van Ginkel ◽  
Charlotte M.A. Lubout ◽  
Bruce H.R. Wolffenbuttel ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin B12 ◽  
Nutritional Deficiencies ◽  
Micronutrient Deficiencies ◽  
Dietary Restrictions ◽  
Normal Ranges ◽  
Correlation Tests ◽  
Tyrosinemia Type 1

Phenylketonuria (PKU) is treated with dietary restrictions and sometimes tetrahydrobiopterin (BH4). PKU patients are at risk for developing micronutrient deficiencies, such as vitamin B12 and folic acid, likely due to their diet. Tyrosinemia type 1 (TT1) is similar to PKU in both pathogenesis and treatment. TT1 patients follow a similar diet, but nutritional deficiencies have not been investigated yet. In this retrospective study, biomarkers of micronutrients in TT1 and PKU patients were investigated and outcomes were correlated to dietary intake and anthropometric measurements from regular follow-up measurements from patients attending the outpatient clinic. Data was analyzed using Kruskal–Wallis, Fisher’s exact and Spearman correlation tests. Furthermore, descriptive data were used. Overall, similar results for TT1 and PKU patients (with and without BH4) were observed. In all groups high vitamin B12 concentrations were seen rather than B12 deficiencies. Furthermore, all groups showed biochemical evidence of vitamin D deficiency. This study shows that micronutrients in TT1 and PKU patients are similar and often within the normal ranges and that vitamin D concentrations could be optimized.

scholarly journals The nutritional, hormonal and vitamin status in overweight and obesity

2017 ◽  
Vol 5 (2) ◽  
pp. 678
Author(s):  
Snaa A. Kamel ◽  
Mohammed K. Alelwan ◽  
Ahmed S. Alqahtani
Keyword(s):  
Vitamin D ◽  
Vitamin B12 ◽  
Weight Control ◽  
Overweight And Obesity ◽  
Nutritional Deficiencies ◽  
High Cholesterol ◽  
Obese People ◽  
Global Epidemic ◽  
Vitamin Status ◽  
Males And Females

Background: Obesity is a global epidemic which is increasing dramatically in overwhelming rates among males and females of all age categories in developing and developed countries.Methods: The first phase of the project involves the questionnaires which apply to all the Participants in research. We also use the lab investigation to determine the abnormality in overweight and obese people. Then we will analysis and combine the results to others research and give output and recommendation that we hope helping us to limiting of increase incidence of obesity.Results: sample show that high cholesterol above normal average approximately in 31.05% of the participants while the triglyceride rate increased in 31.67%. A lack of vitamin D, as the rate did not exceed 18±6.8, in individuals with the BMI 25-29, and to be 13±8.82, in individuals with the BMI>30. Vitamin B12 rate became borderline in individuals with BMI 25-29, and deficient in individuals with BMI>30.Conclusions: Obese Saudi individuals have several nutritional deficiencies specifically in vitamins D and B12 despite their overweight. Efforts should be focused to increase awareness of healthy and weight control.

scholarly journals Imerslund-Grasbeck syndrome- a rare cause of megaloblastic anemia in an infant with homozygous mutation in CUBN gene: a case report and review of the literature

2021 ◽  
Vol 8 (7) ◽  
pp. 1281
Author(s):  
Mehak Agarwal ◽  
Sanmathi Suresh ◽  
Dhaarani Jayaraman ◽  
Sri Gayathri Shanmugam
Keyword(s):  
Vitamin B12 ◽  
Renal Tubule ◽  
Megaloblastic Anemia ◽  
Intrinsic Factor ◽  
Tubular Reabsorption ◽  
Megaloblastic Anaemia ◽  
Nutritional Deficiencies ◽  
Homozygous Mutation ◽  
Review Of The Literature ◽  
Common Causes

Megaloblastic anaemia is one of the important causes of pancytopenia in children and nutritional deficiencies of vitamin B12 and folate are the most common causes comprising 95% of these cases. Defects in absorption, transport and metabolism of vitamin B12 are well described, however, are very rare. We report a rare case of Imersland Grasbeck syndrome, in an infant who presented with pancytopenia, with defective absorption of B12-intrinsic factor complex at the ileum and defective tubular reabsorption of proteins in renal tubule due to same protein defect caused by mutations in two genes – CUBN (cubilin) and AMN (amnionless).

scholarly journals Evaluation of Nutritional Deficiency-5 Years and More Outcomes Post Laparoscopic Sleeve Gastrectomy

2021 ◽  
Author(s):  
Elif Çırak ◽  
Seda Sancak ◽  
Ali Özdemir ◽  
Aziz Bora Karip ◽  
özgen çeler ◽  
...  
Keyword(s):  
Sleeve Gastrectomy ◽  
Vitamin B12 ◽  
Laparoscopic Sleeve Gastrectomy ◽  
Serum Iron ◽  
Vitamin B12 Deficiency ◽  
Nutritional Deficiencies ◽  
B12 Deficiency ◽  
Compliance To Treatment ◽  
Treatment Of Obesity

Abstract IntroductionNowadays one of the most popular surgical method for treatment of obesity is laparoscopic sleeve gastrectomy (LSG). Data regarding long-term nutritional deficiencies following LSG are scare. We aim to assess the prevalence of nutritional deficiencies 5 years post-LSG.MethodsWe retrospectively reviewed the files of 338 patients who had LSG surgery between June 2012 and June 2019 and had preoperative data and had at least 1 year of surgery. Serum iron, ferritin, folic acid, hemoglobin and vitamin B12 levels during annual visits for six years after LSG were compared with preoperative data. Patients with missing data during follow-up were evaluated as dropout and assessments were made on patients with available data.ResultsA total of 338 patients (83% women), with a baseline BMI 46,87±6,17 kg/m2 were included. In baseline, hypoferritinemia was detected in 19.16% for <15 ng/mL cutoff and in 43.11% for <30 ng/ml cutoff value. Serum iron, vitamin B12 and folate deficiencies were exist in 37.62%, 18.64% and 4.14% of the patients, respectively. We compared this prevalances with postoperative datas and found that ferritin deficiency in follow-up visits was significantly more frequent than the baseline (p<0.001), conversely vitamin B12 deficiency was significantly less common (p<0,05).ConclusionDespite the supplement, our results show that the prevalence of patients with hypoferritinemia after LSG is higher than before surgery. This high prevalence may be associated with factors such as insufficient supplementation, insufficient absorption of oral preparations, and insufficient compliance to treatment.

scholarly journals Ergogenic effect of pre-exercise chicken broth ingestion on a high-intensity cycling time-trial

2021 ◽  
Author(s):  
Silvia Barbaresi ◽  
Laura Blancquaert ◽  
Zoran Nikolovski ◽  
Sarah de Jager ◽  
Mathew Wilson ◽  
...  
Keyword(s):  
High Intensity ◽  
Venous Blood ◽  
Time Trial ◽  
Capillary Blood ◽  
Blood Analysis ◽  
Meat Extract ◽  
Blood Samples ◽  
Cycling Time Trial ◽  
Chicken Broth ◽  
Cycling Time

Abstract Background: chicken meat extract is a popular functional food in Asia. It is rich in the bioactive compounds carnosine and anserine, two histidine-containing dipeptides (HCD). Studies suggest that acute pre-exercise ingestion of chicken extracts has important applications towards exercise performance and fatigue control, but the evidence is equivocal. This study aimed to evaluate the ergogenic potential of the pre-exercise ingestion of a homemade chicken broth (CB) vs a placebo soup on a short-lasting, high-intensity cycling exercise. Methods: fourteen men participated in this double-blind, placebo-controlled, crossover intervention study. Subjects ingested either CB, thereby receiving 46.4 mg/kg body weight of HCD, or a placebo soup (similar in taste without HCD) 40 min before an 8 min cycling time trial (TT) was performed. Venous blood samples were collected at arrival (fasted), before exercise and at 5 min recovery. Plasma HCD were measured with UPLC-MS/MS and glutathione (in red blood cells) was measured through HPLC. Capillary blood samples were collected at different timepoints before and after exercise. Results: a significant improvement (p=0.033; 5.2%) of the 8 min TT mean power was observed after CB supplementation compared to placebo. Post-exercise plasma carnosine (p<0.05) and anserine (p<0.001) was significantly increased after CB supplementation and not following placebo. No significant effect of CB supplementation was observed either on blood glutathione levels, nor on capillary blood analysis. Conclusions: oral CB supplementation improved the 8 min TT performance albeit it did not affect the acid-base balance or oxidative status parameters. Further research should unravel the potential role and mechanisms of HCD, present in CB, in this ergogenic approach.

scholarly journals Complications, Reoperations, and Nutrient Deficiencies Two Years after Sleeve Gastrectomy

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Nicole Pech ◽  
Frank Meyer ◽  
Hans Lippert ◽  
Thomas Manger ◽  
Christine Stroh
Keyword(s):  
Sleeve Gastrectomy ◽  
Vitamin B12 ◽  
Single Step ◽  
Nutritional Deficiencies ◽  
Complication Rates ◽  
Nutrient Deficiencies ◽  
Step Procedure ◽  
Vitamin B12 Supplementation ◽  
Single Center Observational Study

Background. The aim of this study was to investigate patient outcomes and nutritional deficiencies following sleeve gastrectomy (SG) during a follow-up of two years.Methods. Over a period of 56 months, all consecutive patients who underwent SG were documented in this prospective, single-center, observational study. The study endpoints included operative time, complication rates, nutritional deficiencies and percentage of excess weight loss (%EWL).Results. From September 26, 2005 to May 28, 2009, 82 patients (female : male = 48 : 34) with a mean age of 43.3 years (range: 22–64) and a preoperative BMI of 52.5 kg/m² (range: 36.8–77.0) underwent SG. Major complications were observed in 9.8% of the patients, with 1 death. During follow up 51.2% of patients were supplemented with iron, 36.6% with zinc, 37.8% with calcium, 26.8% with vitamin D, 46.3% with vitamin B12 and 41.5% with folic acid. %EWL was 54.3, 65.3 and 62.6% after 6, 12 and 24 months.Conclusion. SG as a single step procedure is an effective bariatric intervention. Nutritional deficiencies after SG can be detected by routine nutritional screening. Our results show that Vitamin B12 supplementation should suggest routinely after SG.

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