Expression of cytokeratin 14, cytokeratin 19 and E-Cadherin in ameloblastoma correlates with the cytodifferentiation of enamel organ

Myoepithelium is present in canine mammary tumors as resting and proliferative suprabasal and spindle and stellate interstitial cells. The aim of this paper was to evaluate a panel of markers for the identification of four different myoepithelial cell morphological types in the normal and neoplastic mammary gland and to investigate immunohistochemical changes from an epithelial to a mesenchymal phenotype. Cytokeratin 19 (CK19), cytokeratin 5/6 (CK5/6), cytokeratin 14 (CK14), estrogen receptor (ER), p63 protein, vimentin (VIM), andα-smooth muscle actin (Alpha-SMA) antibodies were used on 29 neoplasms (3 benign and 3 malignant myoepithelial tumors, 7 carcinomas in benign-mixed tumors and 16 complex carcinomas) and on normal tissue of mammary glands. All these antibodies were also tested on 3 mammary tissues from animals with no mammary pathology. The myoepithelial markers were well expressed in the suprabasal cells and gradually lost in the motile types, with the stellate cells maintaining only VIM expression typical of mesenchyma. ER labeled some resting and motile myoepithelial cells. On the basis of our results, we propose a transition from myoepithelial immotile cells into migratory fibroblast-like cells. This transition and the characterization of an immunohistochemical panel for resting and motile myoepithelial cells shed more light on the biological behavior of myoepithelial cells.

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Differential Expression of E-Cadherin and Cytokeratin 19 and Net Proliferative Rate of Gingival Keratinocytes in Oral Epithelium in Periodontal Health and Disease

Journal of Periodontology ◽

10.1902/jop.2007.070070 ◽

2007 ◽

Vol 78 (11) ◽

pp. 2197-2202 ◽

Cited By ~ 20

Author(s):

S. Nagarakanti ◽

S. Ramya ◽

Praveen Babu ◽

K.V. Arun ◽

Sabitha Sudarsan

Keyword(s):

Differential Expression ◽

Cytokeratin 19 ◽

Oral Epithelium ◽

Periodontal Health ◽

Proliferative Rate ◽

Health And Disease ◽

E Cadherin

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Expression of E-cadherin, N-cadherin, and Cytokeratin 18 and 19 in Placentas of Women with Severe Preeclampsia

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.4004 ◽

2020 ◽

Vol 8 (B) ◽

pp. 485-489

Author(s):

Sahar E. Osman ◽

Magdi Salih ◽

Ehab M. Ahmed ◽

Ahmed A. Mohammed ◽

Ishag Adam

Keyword(s):

Case Control Study ◽

Maternity Hospital ◽

Severe Preeclampsia ◽

Trophoblast Invasion ◽

Cytokeratin 19 ◽

Cytokeratin 18 ◽

Significant Difference ◽

And Control ◽

Control Study ◽

E Cadherin

BACKGROUND: Although the exact mechanism leading to preeclampsia is not fully understood, abnormal trophoblast invasion contributes to its pathogenesis. Keratins and cadherin are known to play roles in the regulation of trophoblast proliferation. However, studies describing the association between keratins, cadherin, and preeclampsia are limited. AIM: The current study was conducted to investigate the association of these proteins with severe preeclampsia in Sudanese women. METHODS: A case–control study was conducted at Madani Maternity Hospital, Sudan. The cases included women with severe preeclampsia (n = 56) and healthy pregnant women as controls (n = 56). The assessment of keratin and cadherin was performed using immunohistochemical staining. RESULTS: There was no significant difference between the two groups in their mean age or parity. We found no significant differences in the expression of the markers E-cadherin, N-cadherin, or cytokeratin 18 and 19 in the placentas from individuals with preeclampsia versus controls. The number of placentas with severe preeclampsia versus controls expressing the E-cadherin, N-cadherin, cytokeratin 18, and cytokeratin 19 markers was 46 (82.1%) versus 46 (82.1%) (p = 0.988), 54 (96.4%) versus 48 (85.7%) (p = 0.121), 4 (7.1%) versus 0 (0%) (p = 0.126), and 11 (19.6%) versus 11 (19.6%) (p = 0.532), respectively. There was also no significant difference in the intensity of staining of these four markers (Ecadherin, N-cadherin, and cytokeratin 18 and 19) between severe preeclampsia and control placentas. CONCLUSION: Together, these results indicate that in this setting, the expression of E-cadherin, N-cadherin, CK18, and CK19 is not associated with severe preeclampsia.

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Immunoexpression of HBME-1, High Molecular Weight Cytokeratin, Cytokeratin 19, Thyroid Transcription Factor-1, and E-cadherin in Thyroid Carcinomas

Journal of Korean Medical Science ◽

10.3346/jkms.2005.20.5.853 ◽

2005 ◽

Vol 20 (5) ◽

pp. 853 ◽

Cited By ~ 37

Author(s):

Yoon-La Choi ◽

Mi Kyung Kim ◽

Jin-Won Suh ◽

Joungho Han ◽

Jung Han Kim ◽

...

Keyword(s):

Molecular Weight ◽

Transcription Factor ◽

High Molecular Weight ◽

Cytokeratin 19 ◽

Thyroid Carcinomas ◽

Thyroid Transcription Factor 1 ◽

Thyroid Transcription Factor ◽

Transcription Factor 1 ◽

E Cadherin

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The Immunocytochemistry Is a Valuable Tool in the Diagnosis of Papillary Thyroid Cancer in FNA's Using Liquid-Based Cytology

Journal of Oncology ◽

10.1155/2010/963926 ◽

2010 ◽

Vol 2010 ◽

pp. 1-5 ◽

Cited By ~ 15

Author(s):

Kalliopi Pazaitou-Panayiotou ◽

Nikolas Mygdakos ◽

Kyriaki Boglou ◽

Anastasia kiziridou ◽

Alexandra Chrisoulidou ◽

...

Keyword(s):

Thyroid Cancer ◽

Diagnostic Accuracy ◽

Thin Layer ◽

Papillary Thyroid Cancer ◽

Cytokeratin 19 ◽

Papillary Thyroid ◽

Cervical Lymph Nodes ◽

Galectin 3 ◽

Cytological Features ◽

E Cadherin

Introduction. Papillary thyroid carcinoma (PTC) is the most common malignancy of the thyroid. An accurate cytological diagnosis is based on distinctive cytological features in combination with immunocytochemistry.Methods. A number of 83 fine needle aspirations, positive for papillary thyroid cancer (44 from thyroid nodules and 39 from cervical lymph nodes), were studied using Thin Layer Cytology. A panel of the immunomarkers Cytokeratin-19, Galectin-3, HBME1, CD-44, CD-56, and E-Cadherin was performed.Results. Positive expression of CK-19 was observed in 77 cases (92.7%), of Galectin-3 in 74 cases (89.1%), of HBME1 in 65 (78.3%), and of CD-44 in 72 cases (86.7%). Loss of expression of CD-56 was observed in 80 cases (96.4%) and of E-cadherin in 78 (93.9%).Conclusions. Our data suggest that Thin Layer Cytology increases the diagnostic accuracy in papillary carcinoma and seems to be a promising technique for further investigation of thyroid lesions permitting the possibility to use archive material. Positive immunoexpression of CK-19, Galectin-3, HBME-1, and CD-44 improves the diagnostic accuracy of papillary thyroid cancer. Furthermore, loss of E-cadherin and of CD-56 expression is a feature of malignancy.

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The feasibility of using immunocytochemical research in the preoperative diagnosis of thyroid tumors

Morphologia ◽

10.26641/1997-9665.2020.4.95-100 ◽

2021 ◽

Vol 14 (4) ◽

pp. 95-100

Author(s):

O. I. Zalyubovska ◽

N. O. Hladkykh

Keyword(s):

Thyroid Cancer ◽

Preoperative Diagnosis ◽

Research Work ◽

Elevated Serum ◽

Special Treatment ◽

Cytokeratin 19 ◽

Thyroid Tumors ◽

Cytoplasmic Staining ◽

Strong Expression ◽

E Cadherin

Background. Thyroid cancer has long been an urgent problem of health care in Ukraine and requires both improved timely detection of the disease and increased coverage by special treatment of this category of patients to improve its results. Objective. Investigate the complex of immunocytochemical and ultrasound characteristics of thyroid tumors to improve diagnostic algorithms in the preanalytical stage. Methods. During the the research work the results of diagnostics of 60 patients with thyroid neoplasms aged 21 to 83 years who underwent fine-needle aspiration. Patients were divided into three groups according to the Bethesda System (BSRTC) classification category. The 1st group included 18 patients of category II BSRTC (Benign. Group 2 included 20 patients with category V BSRTC (Suspicious for Malignancy – SFM). And other 22 patients with category VI BSRTC (malignant tumor) formed the 3rd group). Patients of all groups underwent questionnaires, ultrasound examination with FNA, cytological and immunocytochemical examination. Results. There is no clear link between E-cadherin expression and neoplasia. Negative expression occurred in 36.4% of cases of metastatic lesions. This partially satisfies the requirements of timely preoperative diagnosis. That is why E-cadherin cannot be used as a separate marker. The expression of cytokeratin-19 with different degrees of intensity of cytoplasmic staining of tumor cells was determined in all studied neoplasms of V and VI category on the BSRTC scale. Strong expression of cytokeratin-19 was observed in 40.0% of cases with BSRTC-5 and 45.4% with BSRTC-6, moderate - in 50.0% and 36.4% of cases, respectively, weak - in 10.0% and 18.2%, without significant differences between groups (p> 0.05). At the same time, in the benign process in 55.6% of cases there was no reaction of cytokeratin-19, and in 44.4% - weak expression (p <0.001 in comparison with the 2nd and 3rd groups). Conclusion. Benign thyroid disease (category II BSRTC) is likely to be associated with younger patients (46.6 ± 3.7 years), low serum REA (less than 0.95 ng / ml), poor thyroglobulin expression (77.8 %), negative reaction with TTF-1 (100%) and cytokeratin-19 (55.6%). Differential-prognostic markers of thyroid cancer with risk of malignancy (V category BSRTC) include elevated serum REA (0.95 ng / ml and above), the presence of a moderate reaction with antibodies to thyroglobulin (80.0%), a positive reaction - to TTF-1 (100.0%) and E-cadherin (90.0%), with moderate or strong expression of cytokeratin-19 (90.0%).

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