Inhibitory effects of saliva as a suspending fluid on attachment of oral bacteria to hydroxyapatite and titanium

2020 ◽  
Vol 120 ◽  
pp. 104924
Author(s):  
Yi Wang ◽  
Antonia T.W. Lam
Keyword(s):  
Oral Bacteria ◽  
Inhibitory Effects

Inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria

2014 ◽  
Vol 30 (9) ◽  
pp. e238-e244 ◽  
Author(s):  
Éfani C.F. Banzi ◽  
Ana R. Costa ◽  
Regina M. Puppin-Rontani ◽  
Jegdish Babu ◽  
Franklin García-Godoy
Keyword(s):  
Metabolic Activity ◽  
Oral Bacteria ◽  
Inhibitory Effects ◽  
Bonding System

scholarly journals Inhibitory effect of toothbrush monofilament containing surface pre-reacted glass-ionomer (S-PRG) filler on Streptococcus mutans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saaya Matayoshi ◽  
Ryota Nomura ◽  
Takahiro Kitamura ◽  
Rena Okawa ◽  
Kazuhiko Nakano
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Dental Materials ◽  
Self Care ◽  
Inhibitory Effect ◽  
Oral Bacteria ◽  
Glass Ionomer ◽  
Inhibitory Effects ◽  
Oral Environment

AbstractThe oral environment affects not only oral health, but also general health, and the importance of oral self-care has recently been recognised. Although toothbrushes are the most important self-care product, there are few toothbrushes that have an inhibitory effect on oral bacteria. In the present study, monofilaments used for toothbrushes containing surface pre-reacted glass-ionomer (S-PRG) filler (a component recently applied to various dental materials) were developed. Among nylon and polyester monofilaments commonly used for toothbrushes, nylon monofilaments can accommodate more S-PRG filler than polyester monofilaments, resulting in greater release of ions from the S-PRG filler. These monofilaments containing S-PRG filler formed less biofilm containing Streptococcus mutans, a major pathogen of dental caries, than monofilaments without S-PRG filler. Moreover, S. mutans adhering to monofilaments containing S-PRG filler were more easily exfoliated and eliminated than those adhering to monofilaments without S-PRG filler. Such inhibitory effects on S. mutans were more marked in nylon monofilaments than in polyester monofilaments. These findings that monofilaments containing S-PRG filler can release ions and have an inhibitory effect on S. mutans suggest that they may be an effective material for toothbrushes.

scholarly journals Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Soyon Mann ◽  
Myeong Soo Park ◽  
Tony V. Johnston ◽  
Geun Eog Ji ◽  
Keum Taek Hwang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Starter Culture ◽  
Oral Bacteria ◽  
Inhibitory Effects ◽  
Food Ingredient ◽  
Anaerobic Microorganisms ◽  
Oral Pathogens ◽  
Displacement Assays ◽  
Probiotic Microorganism ◽  
Oral Microorganisms

Abstract Background Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body. Results One organism was isolated, named “L. gasseri HHuMIN D”, and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. Conclusion These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

scholarly journals Oral Probiotic Activities and Biosafety of Lactobacillus gasseri HHuMIN D

2021 ◽  
Author(s):  
Soyon Mann ◽  
Myeong Soo Park ◽  
Tony V. Johnston ◽  
Geun Eog Ji ◽  
Keum Taek Hwang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Starter Culture ◽  
Oral Bacteria ◽  
Inhibitory Effects ◽  
Food Ingredient ◽  
Genetic Characteristics ◽  
Anaerobic Microorganisms ◽  
Oral Bacterium ◽  
Oral Pathogens ◽  
Hemolytic Property

Abstract Background Specific probiotic bacteria may have inhibitory effects against oral pathogens. Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research aimed to determine the safety and inhibitory impacts of L. gasseri HHuMIN D as a potential oral probiotic with biotherapeutic capabilities against oral pathogens. Results The microbial and genetic characteristics of L. gasseri HHuMIN D were evaluated in this research. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and L. gasseri HHuMIN D exhibited powerful inhibitory effects on the growth of every harmful oral bacterium tested. Unfortunately, other oral bacteria affected the growth of L. gasseri HHuMIN D, suggesting a poor proliferative response to most co-cultures. Hydrogen peroxide production by L. gasseri HHuMIN D reached 802 µmol/L after 12 hours and gradually diminished until 24 hours. It efficiently aggregated with P. catoniae and S. sanguinis, and completely supressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. L. gasseri HHuMIN D was evaluated for safety using ammonia and biogenic amine development, hemolytic property and mucin degradation testing, antibiotic susceptibility, and whole genome sequencing (WGS). Conclusion Our results suggest that L. gasseri HHuMIN D may be a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

Attachment of oral bacteria to titanium surfaces: An SEM study

1994 ◽  
Vol 52 ◽  
pp. 468-469
Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan
Keyword(s):  
Technical Problem ◽  
Titanium Implant ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Thin Sections ◽  
Surface Ultrastructure ◽  
Sem Study ◽  
Key Factor ◽  
Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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