Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure

2018 ◽  
Vol 269 ◽  
pp. 329-338 ◽  
Author(s):  
Yu-An Chen ◽  
Yan Zhou ◽  
Yanlin Qin ◽  
Dehua Liu ◽  
Xuebing Zhao
Keyword(s):  
Enzymatic Hydrolysis ◽  
Tween 20 ◽  
Ionic Surfactant ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of ◽  
Lignin Structure

scholarly journals Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo

2021 ◽  
Vol 9 ◽  
Author(s):  
Xianqing Lv ◽  
Guangxu Yang ◽  
Zhenggang Gong ◽  
Xin Cheng ◽  
Li Shuai ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrophobic Interactions ◽  
Practical Method ◽  
Tween 20 ◽  
Ionic Surfactant ◽  
Enzymatic Conversion ◽  
Chemical Pretreatment ◽  
Cellulose Accessibility ◽  
Negative Effect ◽  
Hydrolysis Of

Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery.

Research on Enzymatic Hydrolysis of Whey Protein

2013 ◽  
Vol 411-414 ◽  
pp. 3205-3209
Author(s):  
Fang Qian ◽  
Lei Zhao ◽  
Shu Juan Jiang ◽  
Guang Qing Mu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Degree Of Hydrolysis ◽  
Quadratic Regression ◽  
Orthogonal Regression ◽  
Single Factor Analysis ◽  
Verification Test ◽  
Enzyme Dosage ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Based on single factor analysis for the enzymatic hydrolysis of whey protein, papain was selected as the optimal enzyme and its enzymatic hydrolysis conditions were optimized by the quadratic regression orthogonal rotary test. The orthogonal regression model for degree of hydrolysis (DH) to three factors including temperature (X1), time (X2), enzyme dosage (X3) was established as follow: DH=10.40+0.22X1+0.30X2+1.31X3+0.019X1X2+0.011X1X3-0.039X2X3-0.39X12-0.16X22-0.40X32, Verification test showed a DH of 11.7% was obtained at the optimal hydrolysis condition of 56.6°C, 113.8 min and enzyme 8213.7 U /g protein, which basically consisted with the model theoretical value.

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

2016 ◽  
Vol 46 (6) ◽  
pp. 778-790 ◽  
Author(s):  
Ghassan Abo Chameh ◽  
Fadi Kheder ◽  
Francois Karabet
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ph Value ◽  
Maximum Yield ◽  
Olive Pomace ◽  
Mixing Rate ◽  
Content Type ◽  
Hydrolysis Yield ◽  
Pretreatment Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Purpose The purpose of this paper was to find out the appropriate enzymatic hydrolysis conditions of alkali pretreated olive pomace (OP) which enable maximum yield of reducing sugar. Design/methodology/approach The commercial enzymatic preparation (Viscozyme® L) was used for the hydrolysis of OP. The effects of pretreatment, time, temperature, pH, enzyme quantity and substrate loading on the hydrolysis yield were investigated. Findings This study showed that enzymatic hydrolysis of OP using Viscozyme® L can be successfully performed at 50°C. Alkaline pretreatment step of OP prior the enzymatic hydrolysis was indispensable. The hydrolysis yield of alkaline pretreated OP was 2.6 times higher than the hydrolysis yield of untreated OP. Highest hydrolysis yield (33.5 ± 1.5 per cent) was achieved after 24 h using 1 per cent (w/v) OP load in the presence of 100 μl Viscozyme® L at 50°C and pH 5.5 with mixing rate of 100 rpm (p = 0.05). Originality/value Reaction time, temperature, pH value and enzyme quantity were found to have a significant effect on enzymatic hydrolysis yield of alkali pretreated of OP. Although high-solid loadings of OP lowered the hydrolysis yield, it produced higher concentration of reducing sugars, which may render the OP conversion process more economically feasible.

A Study of Biodegradable Superabsorbent Materials Based on Acrylonitrile Grafted Sodium Alginate

2005 ◽  
Vol 277-279 ◽  
pp. 450-454 ◽  
Author(s):  
Young Hee Lee ◽  
Jung Soo Kim ◽  
Han Do Kim
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sodium Alginate ◽  
Graft Copolymer ◽  
Graft Copolymerization ◽  
Water Absorbency ◽  
Grafted Copolymer ◽  
Subsequent Hydrolysis ◽  
Maximum Water ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Biodegradable superabsorbents, hydrolyzed AN(acrylonitrile)-grafted-SA(sodium alginate) copolymers were prepared in this study by graft copolymerization of acrylonitrile on sodium alginate and the subsequent hydrolysis of the resulting grafted copolymer. The absorbency was found to significantly depend on the % add-on, graft copolymerization conditions and hydrolysis conditions. The optimum condition for graft copolymerization to obtain the maximum % add-on (64.5%) was 4g SA, 12g AN, and 8.42g H2O2 in 100ml water at 70 oC for 10hr., respectively. The optimum hydrolysis conditions for the graft copolymer (64.5 % add-on) to reach the maximum water absorbency (2518g/g), saline absorbency (1558g/g), and WRV (288g/g) is 1g graft copolymer in 10 ml aqueous NaOH (1.0N) at 110 oC for 1 hr. Furthermore, this hydrolyzed AN-graft-SA showed a good biodegradability in enzymatic hydrolysis tests when compared with commercial superabsorbent materials.

Effects of Enzymatic Hydrolysis Conditions on the Antioxidant Activity of Red Tilapia (Oreochromis spp.) Viscera Hydrolysates

2020 ◽  
Vol 21 (12) ◽  
pp. 1249-1258
Author(s):  
Cindy T. Sepúlveda ◽  
José E. Zapata
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Protein Concentration ◽  
Polynomial Equation ◽  
Degree Of Hydrolysis ◽  
Red Tilapia ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of ◽  
By Products

Background: Fish is an essential source of nutrients for human nutrition due to the composition of proteins, vitamins, and minerals, among other nutrients. Enzymatic hydrolysis represents an alternative for the use of by-products of the aquaculture industry. Objective: We propose to evaluate the effect of stirring speed, temperature, and initial protein concentration on the degree of hydrolysis of proteins and antioxidant activity of red tilapia (Oreochromis spp.) viscera hydrolysates. Methods: The effect of stirring speed, temperature, and initial protein concentration on the degree of hydrolysis of proteins and antioxidant activity was evaluated using an experimental design that was adjusted to a polynomial equation. The hydrolysate was fractioned to determine the antioxidant activity of the fractions, and functional properties were also measured. Results: Stirring speed and protein concentration presented a statistically significant effect (p <0.05) on all the response variables. However, the temperature did not present a statistically significant effect on the degree of hydrolysis. Discussion: The best conditions of hydrolysis were stirring speed of 51.44 rpm, a temperature of 59.15°C, and the protein concentration of 10 g L-1. The solubility of the hydrolysate protein was high at different pH, and the hydrolysate fraction with the highest antioxidant activity has a molecular weight <1 kDa. Conclusion: The degree of hydrolysis and the biological activity of red tilapia viscera hydrolysates (Oreochromis spp.) are affected by temperature, substrate concentration, and stirring speed. The optimal conditions of hydrolysis allowed to obtain a hydrolysate with antioxidant activity are due to the peptides with low molecular weight.

scholarly journals Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Yanbin Zheng ◽  
Qiushi Chen ◽  
Anshan Shan ◽  
Hao Zhang
Keyword(s):  
Amino Acids ◽  
Enzymatic Hydrolysis ◽  
Incubation Period ◽  
Blood Cells ◽  
Substrate Concentration ◽  
Neutral Protease ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Conditions ◽  
Optimized Conditions ◽  
Hydrolysis Of

For utilizing the blood cells (BCs) effectively, enzymatic hydrolysis was applied to produce the enzymatically hydrolyzed blood cells (EHBCs) by using a neutral protease as a catalyst. The results of the single-factor experiments showed optimal substrate concentration, enzyme to substrate ratio (E/S), pH, temperature, and incubation period were 1.00%, 0.10, 7.00, 50.00°C, and 12.00 h, respectively. The optimized hydrolysis conditions from response surface methodology (RSM) were pH 6.50, E/S 0.11, temperature 45.00°C, and incubation period 12.00 h. Under these conditions (substrate concentration 1.00%), the degree of hydrolysis (DH) was 35.06%. The free amino acids (FAAs) content of the EHBCs (35.24%) was 40.46 times higher than BCs while the total amino acids (TAAs) content was lower than BCs. The scores of lysine (human 0.87; pig 0.97), valine (human 1.42; pig 1.38), leucine (human 1.50; pig 1.90), tyrosine (human 0.84; pig 1.09), and histidine (human 2.17; pig 2.50) indicated that the EHBCs basically fulfilled the adult human and pig nutritional requirements. The calculated protein efficiency ratios (C-PERs) of the EHBCs were 3.94, 6.19, 21.73, and 2.04. In summary, the EHBCs were produced successfully with optimized conditions and could be a novel protein source for humans and pigs.

Optimization of enzymatic protein hydrolysis conditions of Asiatic hard clam (Meretrix meretrix)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 153-162
Author(s):  
M.K. Zainol ◽  
F.W. Abdul Sukor ◽  
A. Fisal ◽  
T.C. Tuan Zainazor ◽  
M.R. Abdul Wahab ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Protein Hydrolysate ◽  
Hard Clam ◽  
Degree Of Hydrolysis ◽  
Protein Hydrolysis ◽  
Meretrix Meretrix ◽  
Optimum Conditions ◽  
Hydrolysis Time ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

This study was aimed to optimise the Alcalase® enzymatic hydrolysis extraction of Asiatic hard clam (AHC) (Meretrix meretrix) protein hydrolysate in terms of hydrolysis time, hydrolysis temperature, hydrolysis pH, and concentration of enzyme. Protein hydrolysate produced from AHC (M. meretrix) meat was used to determine the optimum hydrolysis conditions. Hydrolysis of AHC meat was optimised using the Central Composite Design Response Surface Methodology (RSM) (CCD). The relationship between four parameters such as temperature (45 – 65°C), enzyme to substrate concentration (1 – 2%), hydrolysis time (60 – 180 mins), and pH (7.5 – 9.5) to the degree of hydrolysis was investigated. The optimum conditions for enzymatic hydrolysis of AHC meat to achieve the maximum degree of hydrolysis (DH) were observed at 65°C, enzyme to substrate concentration of 1%, hydrolysis time of 60 mins, and pH 7.5. The enzymatic protein hydrolysis of AHC meat was predicted using a two factors interaction (2FI) model. Under these optimum conditions, DH's predicted value was 97.41%, which was close to the experimental value (97.89%). The freeze-dried protein hydrolysate powder was characterized concerning the proximate composition. Proximate analysis revealed that the AHC meat contains 7.92±1.76% of moisture, 2.23±0.89% of crude fat, 1.98±0.82 of ash, and 10.53±0.04% of crude protein. While the Asiatic hard clam protein hydrolysate (AHCPH) composed 9.12±0.02% of moisture, 0.80±0.29% of crude fat, and 27.76±0.10% of ash. The protein hydrolysate produced also contained high protein content (50.09±0.88%) and may serve as a good protein source.

scholarly journals Effect of Non Ionic Surfactant Addition to Cellulase Performance in High-Substrate-Loading-Hydrolysis of Palm Oil EFB and Water-Hyacinth

2013 ◽  
Vol 13 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Teuku Beuna Bardant ◽  
Sudiyarmanto Sudiyarmanto ◽  
Haznan Abimanyu ◽  
Aisha Kania Hanum
Keyword(s):  
Palm Oil ◽  
Water Hyacinth ◽  
Elaeis Guineensis ◽  
Tween 20 ◽  
Ionic Surfactant ◽  
Similar Reaction ◽  
High Substrate ◽  
Hydrolysis Process ◽  
Hydrolysis Of ◽  
Substrate Addition

Enzymatic hydrolysis with high substrate loading of palm oil (Elaeis guineensis) empty fruit bunch (EFB) and water-hyacinth (Eichhornia crassipes) were investigated as a prior part of ethanol production from lignocelluloses. Commercial surfactant Span 85 and Tween 20 were used as cellulase performance enhancer in hydrolysis process with substrate loading above 20% (w/w). Cellulase performances were compared based on hydrolysis conversion. Hydrolysis conversions of EFB using cellulase with concentration 10 and 15 FPU/g-substrate was 38.55% and 88.80% respectively. Addition 2% (v/v) of Tween 20 to EFB hydrolysis reaction with cellulase concentration 10 FPU/g-substrate gave the conversion 87.30%. This addition enhance the cellulase performance up to 226.5% or similar with the performance of cellulase 15 FPU/g substrate. Addition 2% (v/v) of Span 85 to the similar reaction only enhances cellulase performance to 174.7%. Hydrolysis conversion of boiling-pretreated water-hyacinth and autoclave-pretreated water-hyacinth using cellulase 15 FPU/g-substrate was 45.84% and 52.29% respectively. Addition 2% (v/v) of Tween 20 and Span 85 to boiling-pretreated water-hyacinth hydrolysis with cellulase concentration 15 FPU/g-substrate enhance cellulase performance of 128.9% and 153.5% respectively. Addition 1% (v/v) of Tween 20 and Span 85 to the similar reaction with cellulase concentration 10 FPU/g-substrate gave conversions 51.00% and 53.79% respectively, or similar with conversion of autoclave-pretreated water-hyacinth hydrolysis with 15 FPU/g-substrate.

scholarly journals Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation

2017 ◽  
Vol 65 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Helena Hudečková ◽  
Petra Šupinová ◽  
Libor Babák
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
Optimal Conditions ◽  
Small Particles ◽  
Amylolytic Enzymes ◽  
Total Sugars ◽  
Water Suspension ◽  
Commercial Enzymes ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of

Finding of optimal hydrolysis conditions is important for increasing the yield of saccharides. The higher yield of saccharides is usable for increase of the following fermentation effectivity. In this study optimal conditions (pH and temperature) for amylolytic enzymes were searched. As raw material was used waste bread. Two analytical methods for analysis were used. Efficiency and process of hydrolysis was analysed spectrophotometrically by Somogyi-Nelson method. Final yields of glucose were analysed by HPLC. As raw material was used waste bread from local cafe. Waste bread was pretreated by grinding into small particles. Hydrolysis was performed in 100 mL of 15 % (w/v) waste bread particles in the form of water suspension. Waste bread was hydrolysed by two commercial enzymes. For the liquefaction was used α‑amylase (BAN 240 L). The saccharification was performed by glucoamylase (AMG 300 L). Optimal conditions for α‑amylase (pH 6; 80 °C) were found. The yield of total sugars was 67.08 g∙L-1 (calculated to maltose). As optimal conditions for glucoamylase (pH 4.2; 60 °C) were found. Amount of glucose was 70.28 g∙L1. The time of waste bread liquefaction was 180 minutes. The time of saccharification was 90 minutes. The results were presented at the conference CECE Junior 2014.

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