Genome-wide transcriptome analysis of hippocampus in rats indicated that TLR/NLR signaling pathway was involved in the pathogenisis of depressive disorder induced by chronic restraint stress

2017 ◽  
Vol 134 ◽  
pp. 195-204 ◽  
Author(s):  
Yu Wang ◽  
Huili Jiang ◽  
Hong Meng ◽  
Jun Lu ◽  
Jing Li ◽  
...  
Keyword(s):  
Depressive Disorder ◽  
Signaling Pathway ◽  
Transcriptome Analysis ◽  
Restraint Stress ◽  
Chronic Restraint Stress ◽  
Genome Wide

scholarly journals Antidepressant Mechanism Research of Acupuncture: Insights from a Genome-Wide Transcriptome Analysis of Frontal Cortex in Rats with Chronic Restraint Stress

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Yu Wang ◽  
Huili Jiang ◽  
Hong Meng ◽  
Jing Li ◽  
XinJing Yang ◽  
...  
Keyword(s):  
Frontal Cortex ◽  
Signaling Pathway ◽  
Restraint Stress ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Chronic Restraint Stress ◽  
Rna Seq ◽  
Toll Like Receptor ◽  
Genome Wide ◽  
Receptor Signaling Pathway

Major depressive disorder (MDD) is a chronic disease that adversely affects mood and cognition. In this study, we randomly divided the rats into control group (C), model group (M), fluoxetine group (F), and acupuncture group (A), used open-field test to ascertain whether acupuncture affects chronic restraint stress (CRS) induced depression-like behaviors of rats, and explored the antidepressant mechanism of acupuncture at the molecular level of transcriptome in the frontal cortex of CRS rats by RNA-sequencing (RNA-seq). According to differentially expressed genes (DEG) analysis, we identified 134, 46, and 89 response genes differentially expressed in C versus M, F versus M, and A versus M, respectively. Through Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, we identified the gene sets involved in extracellular space, inflammatory response, Toll-like receptor signaling pathway, chemokine signaling pathway, and TNF signaling pathway. In this study, RNA-seq technology was used to investigate the frontal cortex genome-wide transcriptomes in depression rats under CRS, which suggested that the antidepressant effect of acupuncture is effective and has a multitarget characteristic, which may be related to amino acid metabolism and inflammatory pathways, especially the Toll-like receptor signaling pathway, TNF signaling pathway, and NF-kappa B signaling pathway.

Antidepressant-like effects of albiflorin involved the NO signaling pathway in rats model of chronic restraint stress

2020 ◽  
Vol 18 (11) ◽  
pp. 872-880
Author(s):  
Ying-Li ZHU ◽  
Lin-Yuan WANG ◽  
Dan-Ping ZHAO ◽  
Cheng-Long WANG ◽  
Rui ZHANG ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Restraint Stress ◽  
Chronic Restraint Stress ◽  
No Signaling

Antidepressant-Like Effects of Vaccinium bracteatum in Chronic Restraint Stress Mice: Functional Actions and Mechanism Explorations

2018 ◽  
Vol 46 (02) ◽  
pp. 357-387 ◽  
Author(s):  
Dool-Ri Oh ◽  
Yujin Kim ◽  
Eun-Jin Choi ◽  
Myung-A Jung ◽  
Kyo-Nyeo Oh ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Signaling Pathway ◽  
Restraint Stress ◽  
Forced Swim Test ◽  
Akt Signaling ◽  
Antagonistic Effect ◽  
Chronic Restraint Stress ◽  
Akt Signaling Pathway ◽  
Neuroprotective Effects ◽  
Immobility Time

The fruit of Vaccinium bracteatum Thunb. (VBF) is commonly known as the oriental blueberry in Korea. The aim of this study was to evaluate the antidepressant-like effects of water VBF extract (VBFW) in a mouse model of chronic restraint stress (CRS) and to identify the underlying mechanisms of its action. The behavioral effects of VBFW were assessed in the forced swim test (FST) and open field test (OFT). The levels of serum corticosterone (CORT), brain monoamines, in addition to the extracellular signal-regulated kinases (ERKs)/protein kinase B (Akt) signaling pathway were evaluated. VBFW treatment significantly reduced the immobility time and increased swimming time in FST without altering the locomotor activity in unstressed mice. Furthermore, CRS mice treated with VBFW exhibited a significantly decreased immobility time in FST and serum CORT, increased locomotor activity in OFT, and enhanced brain monoamine neurotransmitters. Similarly, VBFW significantly upregulated the ERKs/Akt signaling pathway in the hippocampus and PFC. In addition, VBFW may reverse CORT-induced cell death by enhancing cyclic AMP-responsive element-binding protein expression through the up-regulation of ERKs/Akt signaling pathways. In addition, VBFW showed the strong antagonistic effect of the 5-HT[Formula: see text] receptor by inhibiting 5-HT-induced intracellular Ca[Formula: see text] and ERK1/2 phosphorylation. Our study provides evidence that antidepressant-like effects of VBFW might be mediated by the regulation of monoaminergic systems and glucocorticoids, which is possibly associated with neuroprotective effects and antagonism of 5-HT[Formula: see text] receptor.

scholarly journals Therapeutic STING activation boosts microglia phagocytosis and ameliorates depression-like behaviors during chronic restraint stress

2021 ◽  
Author(s):  
Qian Zhai ◽  
Yanpeng Zhang ◽  
Shuwen Tan ◽  
Jianyu Sun ◽  
Mao Ye ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Restraint Stress ◽  
Potential Role ◽  
Therapeutic Potential ◽  
Chronic Restraint Stress ◽  
Expression Levels ◽  
Cell Counts ◽  
Latex Beads ◽  
Plus Maze

Abstract Background The STING-TBK1-IRF3 signaling pathway involves in modulating host innate immunity, however, the potential role of STING signaling pathway in chronic restraint stress model has not been determined. The aim of this study is to explore the underlying role of STING signaling pathway in regulating neuroinflammation, as well as to evaluate the therapeutic potential of STING agonist during chronic restraint stress. Methods C57BL/6 mice were subject to 14-day intermittent restraint stress. Sucrose preference, elevated plus maze and tail suspension tests were measured in chronic restraint stress mice. Expression levels of proinflammatory cytokines were tested by QT-PCR and Luminex cytokine assays. The fluorescence-labeled latex beads, flow cytometry and CD68 positive cell counts were utilized to evaluate phagocytic abilities of microglia. Then, the ability of intracerebroventricular injection of STING agonist, 2’3-cGAMP, to reverse the depression-like behaviors and inflammatory cytokines was examined. Results We found that the expression levels of STING, p-TBK1, and p-IRF3 were remarkably decreased in chronic restraint stress mice, which was associated with decreased IFN-β secretion. Moreover, the STING agonist, 2’3-cGAMP, significantly alleviated the neuroinflammation and ameliorated depression-like behavior which depends on the functional STING activation. Furthermore, 2’3-cGAMP promoted microglia phagocytosis through cGAMP-STING-dependent IFN-β release, which was essential for recovery from neuroinflammation during chronic restraint stress. Conclusions These findings demonstrate that STING signaling pathway is a critical mediator in regulating microglia phagocytosis and may serve as a novel therapeutic target for chronic stress-related psychiatric diseases.

scholarly journals Therapeutic STING activation boosts microglia phagocytosis and ameliorates depression-like behaviors during chronic restraint stress

2021 ◽  
Author(s):  
Qian Zhai ◽  
Yanpeng Zhang ◽  
Shuwen Tan ◽  
Jianyu Sun ◽  
Mao Ye ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Restraint Stress ◽  
Potential Role ◽  
Therapeutic Potential ◽  
Chronic Restraint Stress ◽  
Expression Levels ◽  
Cell Counts ◽  
Latex Beads ◽  
Plus Maze

Abstract Background The STING-TBK1-IRF3 signaling pathway involves in modulating host innate immunity, however, the potential role of STING signaling pathway in chronic restraint stress model has not been determined. The aim of this study is to explore the underlying role of STING signaling pathway in regulating neuroinflammation, as well as to evaluate the therapeutic potential of STING agonist during chronic restraint stress. Methods C57BL/6 mice were subjected to 14-day intermittent restraint stress. Sucrose preference, elevated plus maze and tail suspension tests were measured in chronic restraint stress mice. Expression levels of proinflammatory cytokines were tested by QT-PCR and Luminex cytokine assays. The fluorescence-labeled latex beads, flow cytometry and CD68 positive cell counts were utilized to evaluate phagocytic abilities of microglia. Then, the ability of intracerebroventricular injection of STING agonist, 2’3-cGAMP, to reverse the depression-like behaviors and inflammatory cytokines was examined. Results We found that the expression levels of STING, p-TBK1, and p-IRF3 were remarkably decreased in chronic restraint stress mice, which was associated with decreased IFN-β secretion. Moreover, the STING agonist, 2’3-cGAMP, significantly alleviated the neuroinflammation and ameliorated depression-like behavior which depends on the functional STING activation. Furthermore, 2’3-cGAMP promoted microglia phagocytosis through STING-dependent IFN-β release, which was essential for recovery from neuroinflammation during chronic restraint stress. Conclusions These findings demonstrate that STING signaling pathway is a critical mediator in regulating microglia phagocytosis and may serve as a novel therapeutic target for chronic stress-related psychiatric diseases.

scholarly journals The Role of MAPK and Dopaminergic Synapse Signaling Pathways in Antidepressant Effect of Electroacupuncture Pretreatment in Chronic Restraint Stress Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xinjing Yang ◽  
Zhuo Guo ◽  
Jun Lu ◽  
Bingcong Zhao ◽  
Yutong Fei ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Restraint Stress ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Antidepressant Effect ◽  
Control Group ◽  
Group Model ◽  
Chronic Restraint Stress

Acupuncture has demonstrated the function in ameliorating depressive-like behaviors via modulating PKA/CREB signaling pathway. To further confirm the antidepressant mechanism of EA on the mitogen-activated protein kinase (MAPK) and dopaminergic synapse signaling pathways, 4 target proteins were detected based on our previous iTRAQ analysis. Rats were randomly divided into control group, model group, and electroacupuncture (EA) group. Except for the control group, all rats were subjected to 28 days of chronic restraint stress (CRS) protocols to induce depression. In the EA group, EA pretreatment at Baihui (GV20) and Yintang (GV29) was performed daily (1 mA, 2 Hz, discontinuous wave, 20 minutes) prior to restraint. The antidepressant-like effect of EA was measured by body weight and open-field test. The protein levels of DAT, Th, Mapt, and Prkc in the hippocampus were examined by using Western blot. The results showed EA could ameliorate the depression-like behaviors and regulate the expression levels of Prkc and Mapt in CRS rats. The effect of EA on DAT and Th expression was minimal. These findings implied that EA pretreatment could alleviate depression through modulating MAPK signaling pathway. The role of EA on dopaminergic synapse signaling pathways needs to be further explored.

scholarly journals PO-281 Vibration Training Restores Food Intake and Body Weight in a Rat Model of Depression

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Guangcong Peng ◽  
Luodan Yang ◽  
Chongyun Wu ◽  
Jun Hou ◽  
Limei Zhang ◽  
...  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Food Intake ◽  
Depressive Disorder ◽  
Rat Model ◽  
Restraint Stress ◽  
Mental Condition ◽  
Control Group ◽  
Chronic Restraint Stress ◽  
Vibration Training

Objective Stress is well known to negatively affect body weight and food intake in animal models, but the underlying mechanisms have not yet been well elucidated and effective treatment is lacking. This project was initiated to study the potential beneficial effect of vibration training, a novel neuromuscular training method, in the treatment of depression. Methods Adult Sprague-Dawley male rats were randomly divided into the following three groups: 1) naïve control group, 2) depressive disorder group, and 3) depression with vibration training treatment group. To develop a depression phenotype, rats were individually and gently restricted in a modified, well-ventilated tube for 4 h every day for 21 days. Animals in vibration training treatment group were subjected to 30 min of vibration training (30 Hz, 5 days / week) for continuous 5 weeks. Body weight, physical and mental condition, and food intake were recorded daily and the data were statistically analyzed and compared between groups. Results 1. Daily body weight and food intake measurements revealed that both parameters decreased rapidly after the initiating daily restraint stress, compared with control group.  Intriguingly, both body weight and food intake of the depressive disorder group with 5-week vibration training were significantly improved. 2. The secretion of serotonin and dopamine in animals with chronic restraint stress were decreased compared with normal animals, and this attenuation was significantly prevented by vibration training. Conclusions The present study demonstrates that vibration training is capable of restoring food intake and body weight in a rat model of chronic restraint stress-induced depression.

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