TEM analysis and in vitro and in vivo biological performance of the hydroxyapatite crystals rapidly formed on the modified microarc oxidation coating using microwave hydrothermal technique

2019 ◽  
Vol 373 ◽  
pp. 1091-1110 ◽  
Author(s):  
Qing Du ◽  
Daqing Wei ◽  
Shaodong Wang ◽  
Su Cheng ◽  
Yaming Wang ◽  
...  
Keyword(s):  
Microarc Oxidation ◽  
Tem Analysis ◽  
Hydrothermal Technique ◽  
Microwave Hydrothermal ◽  
Biological Performance ◽  
Hydroxyapatite Crystals

scholarly journals Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2081 ◽  
Author(s):  
Teddy Tite ◽  
Adrian-Claudiu Popa ◽  
Liliana Balescu ◽  
Iuliana Bogdan ◽  
Iuliana Pasuk ◽  
...  
Keyword(s):  
High Performance ◽  
Life Time ◽  
Crystalline Lattice ◽  
Current Status ◽  
Synthesis Methods ◽  
Biological Interactions ◽  
Application Range ◽  
Biological Performance

High-performance bioceramics are required for preventing failure and prolonging the life-time of bone grafting scaffolds and osseous implants. The proper identification and development of materials with extended functionalities addressing socio-economic needs and health problems constitute important and critical steps at the heart of clinical research. Recent findings in the realm of ion-substituted hydroxyapatite (HA) could pave the road towards significant developments in biomedicine, with an emphasis on a new generation of orthopaedic and dentistry applications, since such bioceramics are able to mimic the structural, compositional and mechanical properties of the bone mineral phase. In fact, the fascinating ability of the HA crystalline lattice to allow for the substitution of calcium ions with a plethora of cationic species has been widely explored in the recent period, with consequent modifications of its physical and chemical features, as well as its functional mechanical and in vitro and in vivo biological performance. A comprehensive inventory of the progresses achieved so far is both opportune and of paramount importance, in order to not only gather and summarize information, but to also allow fellow researchers to compare with ease and filter the best solutions for the cation substitution of HA-based materials and enable the development of multi-functional biomedical designs. The review surveys preparation and synthesis methods, pinpoints all the explored cation dopants, and discloses the full application range of substituted HA. Special attention is dedicated to the antimicrobial efficiency spectrum and cytotoxic trade-off concentration values for various cell lines, highlighting new prophylactic routes for the prevention of implant failure. Importantly, the current in vitro biological tests (widely employed to unveil the biological performance of HA-based materials), and their ability to mimic the in vivo biological interactions, are also critically assessed. Future perspectives are discussed, and a series of recommendations are underlined.

In vitro and in vivo biological performance of collagen-chitosan/silicone membrane bilayer dermal equivalent

2007 ◽  
Vol 18 (11) ◽  
pp. 2185-2191 ◽  
Author(s):  
Lie Ma ◽  
Yanchao Shi ◽  
Yixin Chen ◽  
Haiguang Zhao ◽  
Changyou Gao ◽  
...  
Keyword(s):  
Silicone Membrane ◽  
Membrane Bilayer ◽  
Dermal Equivalent ◽  
Biological Performance

Applications of ROS-Induced Zr-MOFs Platform in Multimodal Synergistic Therapy

2021 ◽  
Vol 21 ◽  
Author(s):  
Qiongjie Ding ◽  
Yiwei Liu ◽  
Chuncheng Shi ◽  
Jifei Xiao ◽  
Wei Dai ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Efficiency ◽  
Tumor Therapy ◽  
Antitumor Effects ◽  
Metal Organic ◽  
Low Toxicity ◽  
Therapeutic Mechanisms ◽  
Biological Performance

Background: Metal-organic frameworks (MOFs) exhibited the adjustable aperture, high load capacities, tailorable structures, and excellent biocompatibilities that have used to be as drug delivery carries in cancer therapy. Until now, Zr-MOFs in particular combine optimal stability towards hydrolysis and postsynthetic modification with low toxicity, and are widely studied for its excellent biological performance. Introduction: This review comprises the exploration of Zr-MOFs as drug delivery devices (DDSs) with focus on various new methods, including chemotherapy (CT), photodynamic therapy (PDT), photothermal therapy (PTT), sonodynamic therapy(SDT), radiotherapy, immunotherapy, gene therapy and related combined therapies, which all generate reactive oxygen species (ROS) to achieve the high efficiency of tumor therapy. Conclusion: We described and summarized these pertinent examples of the therapeutic mechanisms and highlight the antitumor effects of their biological application both in vitro and in vivo. The perspectives on their future applications and analogous challenge of the Zr-MOFs materials are given.

Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

Rare Metals ◽  
2021 ◽  
Author(s):  
Chun-Sheng Shao ◽  
Liang-Jian Chen ◽  
Rui-Min Tang ◽  
Bo Zhang ◽  
Jiang-Jie Tang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Biological Performance

Plasma Deposition of Biomolecules for Enhanced Biomedical Applications

2015 ◽  
Vol 1723 ◽  
Author(s):  
Liam O’Neill ◽  
Barry Twomey ◽  
Peter Dobbyn ◽  
John O’Donoghue
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Plasma Deposition ◽  
Preclinical Trial ◽  
Minimal Impact ◽  
Plasma Device ◽  
Biological Performance ◽  
The Impact

ABSTRACTBiomolecules have been traditionally immobilised onto surfaces using wet chemical techniques for various medical applications. Recent decades have seen plasma methods being used to prepare these surfaces through various forms of surface modification, but the direct exposure of biomolecules to plasma has been avoided due to fears that the molecules would be denatured by the energetic plasma species. Recent results are now demonstrating that direct plasma deposition of biomolecule coatings can be achieved. This creates the possibility to directly modify the surface of implants without any form of surface pre-treatment and this opens up the possibility to alter the healing processes. Materials such as collagen, chitosan, catalase and heparin can be effectively deposited onto surfaces with minimal impact on biological performance and without any chemical binders, linkers or impurities. The performance of these materials has been characterised using both in vitro and in vivo methodologies. In a further step, the results of a preclinical trial are presented which reveal that direct deposition of biomolecules onto open wounds can also be achieved and the impact of this on wound healing is measured in an immunocompromised animal model. A non-thermal plasma device was used to deliver collagen on to chronic wounds and the treatment was shown to promote wound closure in a rabbit wound healing model.

scholarly journals Characterization and toxicity of citral incorporated with nanostructured lipid carrier

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e3916 ◽  
Author(s):  
Noraini Nordin ◽  
Swee Keong Yeap ◽  
Nur Rizi Zamberi ◽  
Nadiah Abu ◽  
Nurul Elyani Mohamad ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Anticancer Agents ◽  
Drug Loading ◽  
Cancer Drug ◽  
Nanostructured Lipid Carrier ◽  
Tem Analysis

The nanoparticle as a cancer drug delivery vehicle is rapidly under investigation due to its promising applicability as a novel drug delivery system for anticancer agents. This study describes the development, characterization and toxicity studies of a nanostructured lipid carrier (NLC) system for citral. Citral was loaded into the NLC using high pressure homogenization methods. The characterizations of NLC-citral were then determined through various methods. Based on Transmission Electron Microscope (TEM) analysis, NLC-Citral showed a spherical shape with an average diameter size of 54.12 ± 0.30 nm and a polydipersity index of 0.224 ± 0.005. The zeta potential of NLC-Citral was −12.73 ± 0.34 mV with an entrapment efficiency of 98.9 ± 0.124%, and drug loading of 9.84 ± 0.041%. Safety profile of the formulation was examined viain vitroandin vivoroutes to study its effects toward normal cells. NLC-Citral exhibited no toxic effects towards the proliferation of mice splenocytes. Moreover, no mortality and toxic signs were observed in the treated groups after 28 days of treatment. There were also no significant alterations in serum biochemical analysis for all treatments. Increase in immunomodulatory effects of treated NLC-Citral and Citral groups was verified from the increase in CD4/CD3 and CD8/CD3 T cell population in both NLC-citral and citral treated splenocytes. This study suggests that NLC is a promising drug delivery system for citral as it has the potential in sustaining drug release without inducing any toxicity.

scholarly journals Indocyanine Green Loaded Polymeric Nanoparticles: Physicochemical Characterization and Interaction Studies with Caco-2 Cell Line by Light and Transmission Electron Microscopy

Nanomaterials ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 133 ◽  
Author(s):  
Antonella Obinu ◽  
Elisabetta Gavini ◽  
Giovanna Rassu ◽  
Federica Riva ◽  
Alberto Calligaro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Indocyanine Green ◽  
Antitumor Agents ◽  
Polymeric Nanoparticles ◽  
Physicochemical Characterization ◽  
Tem Analysis ◽  
Transmission Electron

Biomedical applications of nanoparticles (NPs) have reached an increasing development in recent years. Recently, we demonstrated that newly synthesized poly (ethyl 2-cyanoacrylate) nanoparticles (PECA-NPs) are possible antitumor agents due to their cytotoxicity for cancer cells. Indocyanine green (ICG), an amphiphilic tricarbocyanine fluorescent dye, is widely used for the detection of tumoral extension in different organs during clinical surgery. Moreover, this fluorescent agent is unstable and it has a rapid clearance in physiological conditions in vivo. In this study, ICG was charged in PECA-NPs to improve its aqueous stability and make easier its use for the identification of tumor cells. Microscopic and ultrastructural aspects concerning the related in vitro interactions between ICG-loaded NPs and tumor cell culture were investigated. Obtained results showed an effective stabilization of ICG; furthermore, color inclusions inside the cells treated with ICG-loaded NPs demonstrated the internalization of NPs with associated ICG. Transmission electron microscopy (TEM) analysis demonstrated the cytoplasmic presence of coated vesicles (Ø ≤ 100 nm), hypothesizing their involvement in the mechanism of endocytosis. Therefore, ICG-loaded NPs could be proposed as agents for tumor diagnosis, hypothesizing also in the future a specific therapeutic treatment.

scholarly journals A novel antibacterial biomaterial mesh coated by chitosan and tigecycline for pelvic floor repair and its biological performance

2020 ◽  
Vol 7 (5) ◽  
pp. 483-490
Author(s):  
Changyan Liang ◽  
You Ling ◽  
Feng Wei ◽  
Lijie Huang ◽  
Xiaomao Li
Keyword(s):  
Infrared Spectroscopy ◽  
Pelvic Floor ◽  
Biological Membrane ◽  
In Vitro Release ◽  
Antimicrobial Effect ◽  
In Vivo Studies ◽  
Pelvic Floor Repair ◽  
Biological Performance

Abstract The biomaterials composed of mammalian extracellular matrix (ECM) have a great potential in pelvic floor tissue repair and functional reconstruction. However, bacterial infection does cause great damage to the repair function of biomaterials which is the major problem in clinical utilization. Therefore, the development of biological materials with antimicrobial effect is of great clinical significance for pelvic floor repair. Chitosan/tigecycline (CS/TGC) antibacterial biofilm was prepared by coating CS/TGC nanoparticles on mammalian-derived ECM. Infrared spectroscopy, scanning electron microscopy, bacteriostasis circle assay and static dialysis methods were used to characterize the membrane. MTS assay kit and DAPI fluorescence staining were used to evaluate cytotoxicity and cell adhesion. The biocompatibility was assessed by subabdominal implantation model in goats. Subcutaneous antimicrobial test in rabbit back was used to evaluate the antimicrobial and repairing effects on the infected wounds in vivo. Infrared spectroscopy showed that the composite coating had been successfully modified. The antibacterial membrane retained the main structure of ECM multilayer fibers. In vitro release of biomaterials showed sustained release and stability. In vivo studies showed that the antibacterial biological membrane had low cytotoxicity, fast degradation, good compatibility, anti-infection and excellent repair ability.

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